Tobacco Documents Online

Toxicity data for all used additives; includes codes.

Proposes continuation of Project B412. Notes recent findings indicate additives increase nicotine migration and previous work shows malic acid added to cigarette paper increases nicotine in peripheral smoke. States project will optimize additives for increased nicotine delivery and controlling smoke pH will result in "satisfactory taste and impact". Suggests additional nicotine chemistry mechanisms studied may yield results for publication. Notes the project objectives: "to optimize migration of nicotine to the periphery of the cigarette,...improve the delivery to mainstream smoke,...understand the mechanisms of nicotine reactions with selected salts and acids." Submits a time-line and man-hours required for technical analysis.

Examines the carcinogenicity of oxolamine citrate and contrasts with the non-carcinogenicity of PMO. Bladder carcinogenesis of oxolamine in animals is identified as related to the diethylaminoethyl group and not the 3-phenyl-1,2,4-oxadiazoyl moiety. Suggests that diethylamine, a urinary metabolite of oxolamine, may be nitrosated in vivo to the carcinogen N-nitrosodiethylamine. Rejects PMO as the source of carcinogenicity.

Details percentage of free and bound nicotine, and smoke pH for Kent III and experimental low-tar cigarettes.

Reports results of CONAP project to enrich reconstituted leaf [RL] with nicotine and study the migration of nicotine from cut tobacco to RL. Lists experimental conditions including: spraying RL with ammonia which was then mixed with cigarette tobacco, use of phosphoric acid and monoammonia phosphate, evaluation of moisture content on nicotine migration, nicotine migration under storage conditions, and elucidating the chemical properties using phosphoric acid treated filter papers. Presents results of nicotine transfer of Kent Gold Light Blend and nicotine transfer properties using various additives. Lists migration of nicotine during aging, leaf analysis of various tobacco grades including 7-74, HL-74, SMEM-75, VLO-75, MX-70, MIIX-70, MIIX-71 and reports leaf pH, %Nicotine, %Nitrogen, % Total Volatile Bases, % Nicotine migration to filter paper and correlates leaf pH, Nicotine, Nitrogen and Total Volatile Bases to nicotine transfer. Recommends further studies to include: quantitation of nicotine migration, migration of nicotine to RL, "migration of nicotine to Cytrel or similar materials", characterizing compounds that co-migrate with nicotine and the migration of nicotine during storage.

Proposes development of methods which distinguish the major bound and free organic acids in tobacco. Indicates organic acids and leaf nicotine may play a role in tobacco off-taste, strength and character. Suggests increased research in this area may provide a means to improve low grade/off-shore burley tobacco smoke qualities.

Reports results of nicotine migration studies "as induced by the presence of malic or tartaric acid." Indicates "impregnation of cigarette paper with acid can cause migration of nicotine to the periphery" causing elevated nicotine delivery into mainstream smoke." Tests levels of nicotine migration and constituent yields based on tobacco and paper types, and other factors.