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2501-2 Aerosol data has been evaluated for a number of cigarette models from several series. Indications are that the mean particle diameter becomes smaller as the amount of sidestream smoke is reduced. Additional data is being studied and a memo will issue describing tkiese evaluations. C. Plans: Chamber runs will be carried out in support of BCR efforts. Following these smokings, the subjective portion of the sidestceam analytical-subjective study will be initiated. A procedure for the analysis of acrolein will continue to be investigated as time pennits. Procedures for collection of sidestteam TPM during normal chamber operation will continue to be evaluated. II. SIDESTREAM SMOKE A. Ob ective: Conduct studies on sidestream smoke including: development of methods for collection and analysis of sidestr+eam semivolatiles and gas phase; visibility detemiinations; analysis of selected materials relating to sidesd+eam odor and itritation; development of proprietary products. B. Results: The prototype CORESTA smoking machine is being used to geaerate mainstream and sidestream TPM and puff count data on a series of eleven cigarette models. This work is in support of the continuing effort to determine why Japanese cigarettes appear to produce less sidestream smoke. Multiple desorber gchns runs were carried out with the new headspace-gc-ms system to determine nm-to-nm variability. The amount of toluene was compared with a single run where the toluene value was arbitrarily set at 100. For 61 runs, the standard deviation at 2 sigma was 40%, the mean was 97.6 and the median was 95.9. Although this is a large variability, most values clustered between 80 and 110. Possible reasons for the variability include changes in the gc split ratio due to changes in the flow through the splitter controller, variations between adsorber tubes and potential problems from the use of a chimney ratber than a known, uniform sample as will be collected from the chamber. All of tbtse potential variables are being addressed. Another experiment was conducted to determine the effect of breakthrough using longer collection times as will be necessary in the chamber. The results showed that at 100 ml/minute for 30 minutes, the breakthrough volume of the desorption tubes is exceeded and that the equilibrium between the Cambridge pad and tube is a variable that will have to be taken into account. However, these observations do not preclude the use of this method to compaie relative amounts of gas phase components for different cigarette models in the chamber. Tubes of different adsorbents in series may be necessary to obtain a true picture of gas phase compositions. C. Plans: The CORESTA smoking of the cigarettes to determine why Japanese cigarettes appear to produce less sidestneam smoke will be completed. An additional series of cigarettes are to be smoked to generate mainstream and sidestream TPM for Aromatek 150 distribution studies. Optimization of the gcJms system will continue and initial chamber collections will begin shortly. 2

4 2501-3 III. MISCELLANEOUS 1. Neutron radiography - The smoking machine at UVa has been modified so that it can take a single lighting'puff and then not puff again on the cigarette. This allows for the running of iandard smolder experiments. The ability to measure the amount of tobacco burned in a cigarette based directly on calibrations on tobacco now exists. Mass burn rates for smoldering IlM13 cigarettes agree to within a maximum of 15% eiror with mass burn rates determined with a balance technique used here at R&D. Linar bum raoas detennined from the radiographic images and from CI agree exactly. The source of the 15% ernor seems to be a number of secondary effects including cannera gain changes during warm-up, density inaccuracies in the calibration procxduiz and the data collection method. All of these items are being addressed and the measurement of mass bum rates accurately from first principles for both static and puffing regimes should be possible. 2. Off-line pycolyses at 300°C and 600°C were carried out on a dithiocazbamate and direct probe mass spectral analyses were conducted on the collected sample and residue remaining in the boat after pyrolysis. Pyrolysis gc/ms analysis of a carboxy- methyl guar gum sample was carried out at 300°C and 6()0°C. Pyrolysis product identifications are in progress. 3. Assistance to personnel of the BCR division continued in their attempts to enzymatically produce N-methylputrescine. Separation and gc/nns analyses of the reaction mixture containing putraxne (starting material) and reaction products failed to identify the desired product. Additional support will continue as requested. 3