Tobacco Documents Online

Prospective Study of Exposure to Environmental Tobacco Smoke and Dysmenorrhea Page 8 of 8 12. Wang X, Tager IB, Van Vunakis H. SpeizerFE, Hanrahan JP. Maternal smoking during pregnancy, urine cotinine concentrations, and birth outcomes. A prospective cohort study. Int J Epidemiol 26:978-988 (1997). 13. Zeger SL, Liang KY. Longitudinal data analysis for discrete and continuous outcomes. Biometrics 42:121-130 (1986). 14. Brown S, Vessey M. Stratton 1. The influence of method of contraception and cigarette smoking on menstrual patterns. Br J Obstet Gynaccol 95:905-910 (1988). 15. Xu X, Li B. Wang L. Gender difference in smoking effects on adult pulmonary function. Eur Respir J 7:477-483 (1994). 16. Baron JA, La Vecchia C, Levi F. The anuestrogenic effect of cigarette smoking in women. Am J Obstet Gynecol 162:502-514 (1990). 17. Khaw KT, Tazuke S. Batrett-Connor E. Cigarette smoking and levels of adrenal androgens in posnoenopausal women. N Engt J Med 318:1705-1709 (1988). ( Articles Online First ) Last Updated: October 5, 2000 http://ehpnet I.niehs.nih.gov/members/20(R)/ 108p I 019-1022chen/chen-full. html 15.1 l.2000

Prospective Study of Exposure to Environmental Tobacco Smoke and Dysmenorrhea Page 4 of 8 you were exposed?" For each menstrual cycle, we calculated an average number of cigarettes smoked per day at home over the entire cycle. This summary variable of ETS exposure at home was used to predict the occurrence of dysmenorrhea during the following menstrual period. It was evaluated as both a continuous and a categorical variable in the subsequent analysis. Exposure to ETS at the workplace was recorded as a binary variable (yes or no). Data analysis. The major question of interest is whether ETS exposure at home independently affects the incidence of dysmenorrhea among women who did not have a history of dysmenorrhea, and whether the relation is modified by the population characteristics and various occupational factors. All data were double entered with Epi-Info (version 6.0; Centers for Disease Control and Prevention, Atlanta, GA, USA). The standard computer program SAS (version 6.12; Cary, NC, USA) was used for data analysis. We first compared the population characteristics by ETS exposure status. We then computed the incidence of dysmenorrhea among prospectively followed menstrual cycles by four ETS exposure subgroups: no exposure and low, medium, and high tertiles of exposure. We also used local regression smoothing scatter plots (S-PLUS, version 3.4, MathSoft Inc., Cambridge, MA, USA) to examine the dose-response relationship between ETS exposure and dysmenorrhea and logistic regression to estimate odds ratios (ORs) and 95% confidence intervals (CIs) of dysmenorrhea associated with "low," "middle," and "high" ETS exposure, adjusting for age, body mass index, education, season, area of residence, occupation, shift work, perceived stress, passive smoking at work, and occupational exposure to chemical hazards, dust, and noise. Generalized estimating equations (GEEs) were then used to account for autocorrelations as a result of multiple cycles per subject (!3). Finally, because the number of observed menstrual cycles varies by subject, we repeated the above analysis with restriction to the first available cycle for each subject. Results This report is based on 165 women, who contributed 625 prospectively followed menstrual cycles with complete baseline and diary infonnation. ETS exposure was reported in 77% of cycles, and the average daily exposures throughout the cycle ranged from 0.02 to 10.3 cigarettes. The incidence of dysmenorrhea was 9.7% and 13.3% among nonexposed and exposed cycles, respectively. Table 1 shows the characteristics of the women by ETS exposure status at the first cycle. The ETS exposed and nonexposed women were similar in terms of the various covariates examined. In this population, the most common method of contraception before enrollment was the use of a condom. At the time of starting the diary collection, 39% of all women reported having used a condom. Only 1.2% of women reported use of oral contraceptives and 7.190 of women ever used an intrauterine or intravaginal device. h ttp://ehpnet t. n iehs.nih. gov/mem be rs/200O/10Sp 1(119- I 022chen/chen-fulI. html 15.1 1.2000

Prospective Study of Exposure to Environmental Tobacco Smoke and Dysmenorrhea Page 1 of 8 Articles . . ........ ............. Environmental Health Perspectives Volume 108, Number 11, November 2000 Prospective Study of Exposure to Environmental Tobacco Smoke and Dysmenorrhea Changzhong Chen,t Sung-I1 Cho,t Andrew 1. Damokosh,t Dafang Chen,t'z Guang Li,3 Xiaobin Wang,4 and Xiping Xut tDepartment of Environmental Health, Harvard School of Public Health, Boston, Massachusetts, USA ZCenter for Ecogenetics, Beijing Medical University, Beijing, China 3Liaoning Antiepidemic Station, Liaoning, China 4Department of Pediatrics, Boston University School of Medicine, Boston, Massachusetts, USA • tc:rndu:•tion . ................... • blethod~ • ~~'4CtfYSton Abstract Dysmenorrhea is a common gynecologic disorder in women of reproductive age. Previous studies have found an association between current cigarette smoking and prevalence of dysmenorrhea. This study investigated the association between exposure to environmental tobacco smoke (ETS) and the occurrence of dysmenorrhea among women without a history of this disorder. The study population consisted of 165 newly wed, nonsmoking Chinese women (in Shenyang, China), who intended to get pregnant and who had no past history of dysmenonhea at the time of enrollment. These women completed a baseline questionnaire interview upon enrollment and were prospectively followed by daily diary. Dysmenorrhea was defined as a diary recording of abdominal pain or low back pain for at least 2 days during a menstrual period. A subject's ETS exposure was defined as the mean number of cigarettes smoked per day at home by household members over an entire menstrual cycle before the menstrual period. A logistic regression model was used to assess the effect of ETS on the risk of dysmenorrhea, with adjustment for age, body mass index, education, season, area of residence, occupation, shift work, perceived stress, passive smoking at work, and occupational exposure to chemical hazards, dust, and noise. Generalized estimating equations were used to account for autocorrelations as a result of multiple cycles per subject_ This report is based on 625 prospectively followed menstrual cycles with complete baseline and diary data. ETS exposure was reported in 77% of cycles, within which average daily exposures throughout the cycle ranged from 0.02 to 10.3 cigarettes. The incidence of dysmenorrhea was 9.7% and 13.3%o among nonexposed and exposed cycles, respectively. Among ETS-exposed cycles, there was a positive dose-response relationship between the numbers of cigarettes smoked and the relative risk of dysmenorrhea. The adjusted odds ratios of dysmenorrhea associated with "low," "middle," and "high" tertiles of ETS exposure versus no exposure were I.1 [95% confidence interval (CI), 0.5-2.6], 2.5 (Cl, 0.9-6.7), and 3.1 (CI, 1.2-8.3), respectively. The findings were consistent with those of analyses limited to the first follow-up menstrual cycle from each woman. These data suggest a significant dose-responsc relationship between exposure to ETS and an increased incidence of dysmenorncea in this cohort of young wnmenKey words: daily diary, dose-response tclationship, dysmenorrhea, environmental tobacco smoke, prospective study. Environ Health Perspect lOR:I(119-1(722 (2000). [Online 5 October2(7t)U] http://ehpnet I.niehs.nih.gov/members/2U00/ lOBp 1019-1(122chen/chen-full.html 15.1 L200t1

Prospective Study of Exposure to Environmental Tobacco Smoke and Dysmenorrhea Page 7 of 8 The biological mechanisms by which cigarette smoke may affect dysmenorrhea are not well understood. Some researchers have suggested that because nicotine is a vasoconstrictor, it can result in reduced endometrial blood flow, which is common in women with dysmenorrhea5o. It is suggested that cigarette smoke may have an antiestrogenic effect (16,17). When the results of this study are interpreted, several methodological limitations should be taken into account. We did not have biochemical measurements of ECS exposure. Our sample size was relatively small and did not allow further analysis of subgroups with recurrent/severe dysmenorrhea or of the timing of ETS exposure within a specific menstrual cycle in relation to the risk of dysmenorrhea. We did not have detailed information on exposure to ETS at work and could not fully assess the joint effect of ETS exposure at home and at the workplace. Our measurement of potential confounders was based on self-report rather than objective measurements, which may have limited our ability to control some confounders. Finally, although we excluded women with a history of dysmenorrhea, we did not have clinical information of unrecognized gynecological disorders that may produce new dysmenorrhea symptoms- Although our findings from observational study are biologically plausible and highly consistent, they are only suggestive, not conclusive. Further studies are needed to corroborate our findings. From a public health perspective, dysmenorrhea is a highly prevalent gynecological morbidity accounting for significant medical cost, absenteeism, lost working time, and reduced quality of life. Identification of modifiable risk factors of dysmenorrhea has important implications for women's reproductive health. Our study findings reiterate that preventing both active and passive smoking among women of reproductive age is likely to have a significant beneficial impact on their reproductive health. References and Notes 1. Ylikorkala 0, Dawood MY. New concepts in dysmenorrhea. Am J Obstet Gynecol 130:833-847 (1978). 2. Andersch B, Milsotn 1. An epidemiologic study of young women with dysmenorshea Am I Obstet Gynecol 144:655-660 (1982). 3. Klein JR, Liu ff'. Epidemiology of adolescent dysmenorrhea Pediatrics 68:661-664 (1981). 4. Harlow SD, Park M. A longitudinal study of risk factors for the occurrence, duration and severity of menstrual cramps in a cohort of college women. Br J Obsmt Gynaecol 103:1 134-1142 (1996). 5. Dawood MY. Dysmenorrhea. I Reprod Med 30:154-167 (1985). 6. Friederich MA. Dysmenorrhea. Women Health 8:91-106 (1983). 7. Kennedy S. Primary dysmenorrhoea. Lancet 349:1116 (1997). 8. Hornsby PP, Wilcox AJ, Weinberg CR. Cigarette smoking and disturbance of inensuual funetion. Epidemiology 9:193-198 (1998). 9. Parazzini F, Toui L, Merzopane R, Luchini L, Marchini M, Fedele L. Cigarette smoking, alcohol consumption, and risk of primary dysmenorrhea Epidemiology 5:469-472 (1994). 10. Sundell G. Milson 1, Andersch B. Factors influencing the prevalence and severity of dysmenorthea in young women. Br J Obstet Gynecol 97:588-594 (1990). 11. Sloss EM, Frerichs RR. Smoking and menstrual disorders. Int J Epidemiol 12~ 107-109 (1983). http J/chpnet 1. n iehs.nih. gov/members/2 W0/ l O8p 10 19- I(122che n/chen-ful l. html 15.1 1.2OOU

Prospective Study of Exposure to Environmental Tobacco Smoke and Dysmenorrhea Page 3 of 8 pain (8-l0). Nulliparous women were reported to have a higher prevalence of dysmenorrhea than multiparous women. In our study, all the subjects were newly wed, nulliparous, nonsmokers who intended to conceive (thus, they used no contraceptives during the follow-up menstrual cycles). These characteristics minimize the confounding effects of contraceptive use, parity, and active smoking. This study also adjusted for a number of occupational exposures that may be associated with dysmenorrhea. Methods Study population. Beginning in July 1996, we established a cohort of newly wed couples in two districts (Tiexi and Dadong) in Shenyang, China. These couples were recruited to participate in a comprehensive prospective study designed to investigate the effects of various environmental and occupational exposures on reproductive outcomes. Newly wed couples were enrolled when they had obtained permission to become pregnant. Using daily diaries, these couples were followed until occurrence of clinical pregnancy or for up to 1 year. Women who had a prior marriage or a medically diagnosed gynecologic or endocrine disease were ineligible. Eligible subjects were recruited upon their consent and participated at a rate of > 90%. In total, 472 women have enrolled, and among these; 274 reported having history of dysmenorrhea (58.1%). For the current analysis, we defined the ETS exposure for each complete menstrual cycle according to the diary, which was used to link with the occurrence of menstrual pain in the next followed up menstrual period. Therefore we only included 388 (82.2%) women who had been followed for at least one complete menstrual cycle. Of the 388 women, 223 reported having history of dysmenorrhea (57.5%) and were excluded from the analysis for the purpose of examinining the effect of ETS exposure on the incidence of dysmenorrhea. The final analysis was based on 165 women who did not report a history of dysmenorrhea at baseline interview and who had at least one complete menstrual cycle. This study was approved by Institutional Review Boards at Harvard School of Public Health and Beijing Medical University. Baseline survey. We used the Chinese marriage registration system to identify newly wed couples and those planning a first pregnancy. Upon enrollment, physical examination was performed, and height and weight were measured according to a standard protocol. A structured baseline questionnaire was administered by a trained interviewer to all the women and their husbands at enrollment to collect information concerning occupational exposures, personal habits such as cigarette smoking and alcohol consumption, living environment, exposure to passive smoking, dietary intake, menstrual and reproductive history, and contraceptive use. Daily diary. The current analysis is a part of longitudinal study to examine reproductive outcomes including fecundity. Each woman was followed monthly by the field staff. When a woman decided to stop contraception in order to become pregnant, she began recording a daily diary on menstrual bleeding and associated symptoms and exposure to tobacco smoke and other occupational exposures. Daily diary collection was terminated if a woman became clinically pregnant, dropped out of the study, or did not become pregnant within I year of cessation of contraception. Assessment of dysmenorrhea A menstrual cycle was defined from the first day of menstrual bleeding to the day immediately before the next menstrual bleeding. Menstrual pain was defined as abdominal pain or low back pain during menstrual bleeding. In this report, dysmenotrhea was defined as 2 or more days of menstrual pain during menstrual bleeding. From the baseline questionnaire, a positive history of dysmenorrhea was defined as any menstrual pain during the previous 12 months. Assessment of environmental tobacco smoke Each woman recorded in her daily diary the number of cigarettes smok0d by the regular household members. The specific question in the diary t'orm was, "What is the number of cigarettes someone smoked indoors at home yesterday while http://chpnet t .niehs.nih.gov/members/200O/108p 1019-I022chen/chen-full.html 15.11.2000

Page 1 of I Table 1. Characterlstics of 155 women by pesslva smokln0 status at Vte first follow-up menstrual cycle, Sheiqaiq. China. Pauivesmnldn athane Cheracteristlcs No n• asin= 1321 p-Value• Physisal Apebeers) 25.912.3 26.112.6 0686 Heiple(m) 162,1001 1 1.601016 0.046 WaqN p.y) 54.116.6 54.0 17.4 0.943 00 mass Index (kp/rrP) 20.6s 2.8 21.0125 8465 Arje at Mmnylo Nears) 14.1413 141111 1.4 p.627 Eorcatlon Mildleschoolorlrnvei 212 235 0.911 H4hahwal 42.4 43.9 Collape ar abrr2 38.4 32.6 Oaupetlou Tatrdckn 51.5 47.0 0.092 Adminhpafne saff 242 273 factaywarker 249 257 llccupatbrtal ezposufe Toth 3.7 8.5 8400 Ndso 14.8 184 0.120 Oid 00 6.5 0.449 Shiflwork 27.3 280 8882 Perceipetl stress 9.1 13.7 0.725 Pastbosmdcingalwork 3'i3 45.4 0.250 Soasan tollowup sfa r1ed SptlnO 394 288 0.8;ip Summar 12.1 189 rdl 30.3 32B Winter 182 19.7 No. ot pcies lolowed 1 24.3 23.5 0.961 24 515 47.7 5-7 12.1 13.6 >0 12.1 152 Bcropt m romQ el vaWat ara pxcart ot tiel aubJactx otrarvaWar are maen t SB p-Vduas forphylicrl charactwislin aro dcrind byf•frst;ae ethuraero dqirad bydrl-tqurra ba[ 69Z189S05Z http://ehpnetl.niehs.nih.gov/members/2000/ 108p 1019-1022chen/chentab 1 B.GIF 15.11.2000

Prospective Study of Exposure to Environmental Tobacco Smoke and Dysmenorrhea eGr.C.a o .. a.w P.Wr e. rr sl... r• nl Lir ln.non. iM1.,.rq~ ~M1sw Y. rl~.r. Crr.cv.wc[ reF-iR ~~ 1rN-li} 4V.Yw I1.,aN +tpYr.) m.n 21,1.21 lol 4VI1N 151,11" 110,11% lrG W.ylnyl Gll.Gr Sal.]a 9113 h,l•1i 2114,211 ALIS rsc ~y.n~..rh.... 111.13 uo.1a om .r. YY/Yn/./rYmsr Al ]1f Glll ~sdwl Ra 111 C.r1u.W 314 221 yAr Tr~ Glc ue Iw1 wr;rr.~w,. xs As G.eryrb Mf Xl wyf,nl.n.,n x : 37 al rm r.. lu ua ua b r./ II ~rl srl..n aI A/ I Ire ..w,.r. u ui otc xnYff, rvM A] Ka IiGI .,n 41.rq nr./ J'iq 1i1 ]Ir rl]I d.m- 111 111 rM a1 ul Mrm lz I] ael clzrfirwr ]u 239 im 2-l nS l1] 6..} 12.1 11e •1 111 IG] ~arl.rnua~~orrm o:w.r....~aa vwnr www.r.,rn..rrr rwrar.rnr .w i..rY. Page 5 of 8 The overall incidence of dysmenorrhea was 12% among T.61e 2. Crude incidence rate of tlysmenorrhea In all ros ectivel observed menstrual c cles. However, as the 625 fohow-up menstrual cl cles by pasefve P P Y Y smoldnp ststus. shown in Table 2, the incidence differed significantly by the four ETS exposure categories, with the lowest rate (9.7%) in the nonexposed group and the highest rate (16.9%) in the high ETS exposure group. Among ETS- exposed cycles, there was a positive dose-response gradient between the number of cigarettes smoked and the incidence as well as the relative risk of dysmenorrhea. Table 3 presents the crude and adjusted ORs of dysmenorrhea in relation to ETS exposure. Compared with cycles without ETS exposure, the adjusted ORs of dysmenorrhea associated with low, middle, and high PaseivesmaldnU Total Drstnenorrhea (ciparettestaay) cycles No. Percent All qdes Nole 145 14 9.7 tow(<6e) 160 15 9.a Mitltlle (O.B-2.5) 160 zt 13.8 H iy h[> 2.15) 166 27 169 Fist tycls alq None 33 2 6.1 mw (< 0.8) 44 5 11.4 Mldtlle (0 &.2 7) 44 7 15.9 Hlgh (>2.0) 44 17 29.6 tertiles of ETS exposure were 1.1 (Cl; 0.5-2.6), 2.5 (CI, 0.9-6.7), and 3.1 (Cl, 1.2-8.3), respectively. The findings were again consistent when the analysis was limited to the first menstrual cycle from each woman. Three variables as a group--namely age, body mass index, and occupational category--explained most of the difference between the crude and adjusted results. When these three variables were excluded from the full model, the ORs for the exposure categories were 1.08, 2.20, and 2.46, respectively, leading to results similar to the unadjusted effect estimates. However, exclusion of any single variable from the model did not lead to such a change from the full model. When ETS exposure was treated as a continuous variable, the estimated OR of dysmenorrhea was 1.3 (Cl, L0-1.8) for exposure to two cigarettes per tiay; that is, the risk of dysmenorrhea increased by 30% for each two cigarettes smoked at home. Finally, we performed several subset analyses to exclude the possibility of potential confounding. We tirst excluded the women who had used either oral contraceptives or an intrauterine device before the study. The ORs of dysmenorrhea for women with low, middle, and high exposure to ETS were 1.I (Cl, 0.5-2.6), 2.6 (CI, 0.9-7 1), and 3.1 (Cl, 1.2-8.2), respectively, which were consistent with those from the total sample. http Jleh pnet 1. niehs.nih. gov/members/2000/ 108p I(119-1022che nlchen-f ull- h(ml 15.1 1.2000

Prospective Study of Exposure to Environmental Tobacco Smoke and Dysmenorrhea Page 6 of 8 Table 3. Crude and adjusted odds ratios af dysmenorrhea associntotl with passive smoking from 625 fdlaw-up cyGas. Passivesmotlng Unadlusletl Iidjusted• (dgarettssdayry N ~%CI p-Value OR 96%CI )tYaue Moda I ntone 1.0 - - 1.0 - - (nw(<0.8) . 1.1 0.5-2.5 0.041 1.1 OS-2.6 0.801 Middkl.0.B-25) 2.1 0."6 0.150 2.5 0.9-8.7 0.079 Hiph(>1.8) 2.4 -0.8-6.1 0.072 3.1 1.2-8.3 0-024 Mode1 11 Tsw riparettra/dar 1.3 1.0-1.6 0.088 1.3 1.0-1.8 0.030 'rha IogNgc nnroasioo modal adju.iad far dizarict (Timd va Oadnegj, ega tartNa (21.5-21.8, tt7-7B.5, 28S31.1t hody maf. indox tat4ht fti4-19.0.198-7 t.9, 23A-91 it eW cation fmiddle achml or Iowvr, high schaol, rollago ur ehwej, eroa of roeidann, occupation (tacMkien, admlNatratka etaf( hu:tmy workeu), apiftwodc, parcgkad ma.; occupauatd afcpesua tn cluniral hamFda, imw and dutt pafafw emokfng at warQ and tlho aanaon twkdaraM eprYg ee, sumwr and [sp) of Lu stem.g c9cle. Ganarallad aaimetingaplatlanswera uaadta accuumfurmulipb qckatparwoman. The number of available cycles varied by each individual woman. Moreover, both dysmenorrhea and ETS have a high intraclass correlation coefficient within cycles (0.44 and 0.67, respectively), and therefore women with fewer cycles will have smaller weight in the evaluation of passive smoking effect. We addressed this potential bias by only including each woman's first menstrual cycle in the analysis. As shown in Table 2, the results were similar to those of the analysis that included all available cycles. Exposure to ETS at work was another potential confounder. Because we did not have detailed information on workplace exposure, we could only include it as a binary variable (yes, no) in the regression analysis. ETS at work may also be considered as a component of exposure. To address this concern, we first restricted our analysis to women who did not report exposure to ETS at work at the baseline interview. The results remained consistent; the ORs of dysmenorrhea for low, middle, and high passive smoking were 1.1 (CI, 0.4-3.5), 1.9 (CI, 0.6-6.3), and 2.0 (CI, 0.6-6.7), respectively. Second, we combined ETS at home (no, low, middle, high) with ETS at work (no, yes), creating eight categories of exposure. Compared to the group with neither exposure, the ORs for the groups were 1.3 (low, no), 0.9 (low, yes), 2.5 (middle, no), 2.4 (middle, yes), 3.0 (high, no), and 3.1 (high, yes). Discussion Previous studies demonstrated the adverse effects of current smoking on menstrual cycle (S,8- 10,14). Few studies are available on ETS exposure in relation to the risk of dysmenorrhea. In this report, we found a significant dose-response relation between ETS exposure and the risk of dysmenorrhea, even after adjustment for important covariates. It is noted that our study reported the incidence rate of dysmenorrhea among those who did not have a history of dysmenorrhea at enrollment Most previous studies reported prevalence. According to the baseline questionnaire, the prevalence of dysmenorrhea in our population was 58.1%, which is comparable to the prevalence reported in the previous studies (47-72%). The effect of passive smoking on dysmenorrhea observed in this study appears stronger than that in a longitudinal study by Hornsby et al. (8), in which the authors observed a 30% increase in the mean number of days with dysmenorrhea. Some features of our study may contribute to a stronger effect compared to the previous studies. Our study population was younger than those included by Hornsby et al., who included women 37-39 years of age. We measured the amount of ETS by estimating the number of cigarettes smoked in the women's presence, whereas Hornsby et al. defined ETS as living or sharing a workplace with a smoker. In addition, our study focused on new occurrence of dysmenorrhea, excluding women who reported a history of dysmenorrhea. All the women in our study were nonsmokers and thus current smoking was not a confounder here. httpl/ehpnetl.niehs.nih.gov/membersl2(7(X)/108p1019-1022chen/chen-full.html (5.11.20(X)

Prospective Study of Exposure to Environmental Tobacco Smoke and Dysmenorrhea Page 2 of 8 Address correspondence to X. Xa Department of Environmental Health, Harvard School of Public Health. 665 Huntington .4venue, Boston, MA 02115 USA. Telephone: (617) 432-2959. Fax: (617) 432-2956. E-mail: zu@bsph harvard edu This study was supported in part by grants 1R01 ES08337 from the National Institute of Environmental Health Sciences and IROI HD32505 from the National Institute of Child Health and Human Development. 1). Chen was supported in part by a Fogarty International Center training grant (TW00828). Received 24 Apri12000; accepted 13 June 2000. Introduction Dysmenorrhea, or painful menses, is a common gynecologic disorder in women of reproductive age. Reported prevalence ranges from 47 to 72% for various age groups (1-3). This condition accounts for significant school absenteeism, lost working time, and reduced quality of life¢6). In the United States it is estimated that 600 million working hours are lost annually as a result of primary dysmenorrhea (5), and it is now recognized as an important women's health issue Q). A growing body of evidence demonstrates an association between environmental and occupational exposures and adverse reproductive outcomes. Although limited data are available for dysmenorrhea, epidemiological studies have shown a link between dysmenorrhea and several environmental risk factors, including current cigarette smoking #-//). An even greater proportion of women are exposed passively to environmental tobacco smoke (ETS). The question of whether exposure to ETS also increases the risk of dysmenorrhea has been studied only to a limited extent. In a study by Hornsby et al. (8), women exposed to ETS reported more days with dysmenorrhea on average (2.6 days) compared to unexposed women (2.0 days), adjusting for duration of menses and other confounders. There are a number of methodologic challenges in studying the health effects of ETS exposure. First, quantification of ETS exposure has been problematic, in part because such exposure takes place in multiple locations. The contribution of each environment to total personal exposure varies with the amount of time spent and the concentration of ETS. Most studies are retrospective or cross-sectional in nature, so that the validity of these data depends on the accuracy of a subject's recall. Furthermore, a temporal relation cannot be established, and thus causal inference cannot be made. As estimated by a previous study (2) using urine cotirtine as a biomarker of cigarette smoke exposure, current smoking may result in urine cotinine levels 30- to 100-fold higher than ETS. It is likely that the magnitude of effect from ETS is small and more difficult to detect than that of current smoking. Among smoking women who are also exposed to ETS, it is difficult to sort out the independent contribution of current and passive smoking. The purpose of this report is to examine the association of ETS exposure with dysmenorrhea among a cohort of newly wed, nonsmoking women who had no history of dysmenorrhea in Shenyang, China. Our study has several unique features. Dysmenorrhea and exposure to ETS were defined by the use of a daily diary. This prospective study design eliminates potential recall bias, a common drawback of retrospective or cross-sectional studies. In China, few women smoke cigarettes, but exposure to ETS is very high because of the high prevalence of smoking among men (I5). This setting provides a good opportunity to study the effect of ETS exposure on dysmenorrhea. Oral contraceptives were sometimes used clinically to alleviate menstrual pain, thus introducing a potential confounder (/0). Parity was also suggested to be associated with menstrual http://chpnet I. nich s n ih _ g ov/inembc rs/2000/ l ORp 1 O I 9-1022che n/chen-full. h tm l 15.1 1.20(X)