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I I I I I I I I I I I I I I I I A STUDY OF THE RELATIONSHIP BETWEEN P53 MUTATION AND SMOHING IN HUMAN NON-SMALL CELL LUNG CANCER Li Jin-han, Bi X.J. and Peng Z.H. Department of Oncology, Nan Fang Hospital, First Military Medical University, Guangzhou, China A study was undertaken during 1992 to 1993 to explore the relationship between p53 mutation and cigarette smoking in human non-small cell lung cancer (NSCLC). The group of 43 males and 9 females, with age ranging from 30 to 71 years (median, 55), consisted of 30 squamous cell carcinomas and 22 adenocarcinomas. The detection of p53 mutations of all primary resected NSCLC specimens was performed using inununohistochemistry. One patient with adenocarcinoma had a mutated allele of p53. PCR-SSCP analysis showed the p53 mutation to be in the region between exons 5, 6, 7 and 8. Sequence determination revealed that codon-280 had been changed from AGA to ACA. Thirty-seven of the 52 lung cancer patients were self-reported smokers, suggesting that the presence of lung cancer was probably associated with smoking. Among the 25 patients with a p53 mutation, the smokers accounted for (74%) 17 cases (68%), which was not significantly different from the 20 smokers in the group of 27 showing no mutation in the p53 gene. These results implied that the p53 mutation may not be closely related with smoking cigarettes. O ~ ~ V ' 00 W W 00 ~ I