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I I I I I I I I I I I I I I I I I I # 107 AUTHOR: WEHNER, ALFRED P., GERALD E. DAGLE, EDWARD M. MILLIMAN, DANIEL W. PHELPS, DANIEL B. CARR, JOHN R. DECKER, AND RONALD E. FILIPY DATE: 1981 TITLE: INHALATION BIOASSAY OF CIGARETTE SMOKE IN RATS CITATION: TOXICOLOGY AND APPLIED PHARMACOLOGY 61, 1-17 (1981) STUDY DESIGN; Groups of Fischer 344 female rats were exposed to cigarette smoke from three types of cigarettes (high tar - low nicotine; low tar-medium nicotine, and high tar-high nicotine) in Maddo×-ORNL smoking machines, eight cigarettes/day, 7days/week, for up to 24 months. An additional group received sham exposures and a fifth grou served as untreated controls. RNDINGS/RESULTS: The sham-exposed animals had significantly lower body weights than the untreated controls. The smoke-exposed animals had significantly lower weights than the untreated controls; the weights were lower for the low tar-medium nicotine and the high tar-high nicotine animals than for the high tar-low nicotine rats during the second year of exposure. The survival of the high tar-low nicotine animals was similar to that for the sham-exposed and untreated control group; survival times of the low tar-medium nicotine and the high tar-high nicotine dose groups were shorter. Body weight and survival reflected the high and low nicotine dose groups indicated by in vivo dosimetry measurements. Smoke induced histopathologic lesions consisted primarily of pulmonary smoke granulomas; the smoke granulomas were less severe in the low tar-medium nicotine group than in groups exposed to smoke from the other cigarette models. Additional changes included pulmonary alveolar epithelial hyperplasia, and squamous metaplasia and basal cell hyperplasia, and squamous metaplasia and basal cell hyperplasia of laryngeal and tracheal epithelium, One primary epidermoid carcinoma was found in the lung of low-tar-medium nicotine rats. CONCLUSIONS; The rats tolerated the chronic exposures relatively well and certain of the smoke-induced lesions allowed differentiation between the different types of cigarettes. I