Tobacco Documents Online

! I I I I I I I I I I I I I I I I I ~93 AUTHOR= ROGERS. WALTER R., BRUCE McCULLOUGH AND JOHN E. CATON DATE: 1981 TITLE= CIGARETTE SMOKING BY BABOONS: IN VIVO ASSESSMENT OF PARTICULATE INHALATION USING BRONCHOALVEOLAR LAVAGE TO RECOVER [14C]DOTRIACONTANE. CITATION: TOXICOLOGY, 20 309-321 (1981) STUDY DESIGN= A bronchoalveolar lavage method for the recovery of ['~C]dotriacontane was developed in order to demonstrate quantitatively that cigarette smoking baboons inhale ~rticulate matter into the lung. First, 9 baboons were exposed to a known dose of [ C]dotfiacontane labeled particulate matter delivered in a manner providing extensive deposition of particulates in the lung. The lungs of these passively exposed animals were then lavaged so that the efficiency of recovery of the standardized lavage procedure could be determined. Second, 9 baboons actively smoked labeled cigarettes, and the lungs of these animals were lavaged to recover labeled [14C]dotriacontane. RESULTS/FINDINGS: The total amount of particulate matter present in the lungs,was estimated using the efficiency factor previously determined. The smoking baboons retained an average of 9% of the total cigarette particulate matter. Differences among animals in retention of particulate matter were considerable, and the inter-animal variability was related to differences in number, volume, duration, and pressure of puffs. CONCLUSIONS; The retention of particulate matter by baboons is similar to particulate retention by other animal smoke inhalation models.