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I I I I I I I i I i i AUTHOR; MATULIONIS, DANIEL H. DATE:1984 .T.LT_~: CHRONIC CIGARETTE SMOKE INHALATION AND AGING IN MICE: 1, MORPHOLOGIC AND FUNCTIONAL LUNG ABNORMALITIES C;ITATION; EXPERIMENTAL LUNG RESERACH 7:237-256 (1984) STUDY DESIGN; _C57BL/c male mice were used in this study. At the onset of the experimetn 48 mice were 2 months old (young animals) and 62 were 8-10 months old (old animals. Smoke exposure of animals occurred in the THRI (Tobacco & Health Research Institute) single-port reverse smoking machine, twice a day to 10 puffs of smoke (diluted such that they were exposed to 20% smoke, from one 2A1 University of Kentucky reference cigarette. Sham treatment consisted of exposing animals to air instead of smoke. Treatment period lasted for 9 months. FIND!NGSIRESULTS: Microscopic and morphometric evaluation of pulmonary tissue revealed peribronchiolar and perivascular accumulations of lymphocytes and macrophages in lungs of both young and old smoke-exposed mice. Such lesions were never observed in sham treated or control animals of either age, These observations indicate that lesions in mice can be induced by long-term smoke inhalation. In addition, three other anomalous manifestations, prominent only in smoke-exposed old animals, were noted; reduction of alveolar space with a concomitant increase in lung cellularity and thickened alveolar septa; intra-alveolar accumulation of surfactantlike material and decreased pulmonary function. GONGLUSIONS: Pulmonary function test substantiated the morphologic and morphometric data, which suggest that abnormal conditions noted manifest as a result of the interaction between smoke inhalation and aging. Additionally, the manifestation of abnormality was noted in the old smoke-exposed animals are restrictive in nature and conform most closely to pulmonary fibrosis.