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MONDAY MORNING - SEPTEMBER 26,1994 Joint Session 8:30 WELCOME AND OPENING REMARKS 8:45 TRIBUTE TO RAY SEVERSON: D. Michael Jackson, USDA-ARS, Crups Research Laboratory, Oxford, NC 27565, USA 8:55 RECENT ADVANCES IN TOBACCO SCIENCE SYMPOSIUM: "POSITIVE ASPECTS OF SMOKING" INTRODUCTION: John H. Robinson,Symposium Chairman, R. J. Reynolds Tobacco Company, Research & Development, Winston-Salem, NC 27102, USA 9:00 1. NICOTINE UPTAKE AND METABOLISM IN SMOKERS. G. D. Byrd, J. H. Robinson, W. S. Caldwell, and J. D. deBethizy, R. J. Reynolds Tobacco Co., Research & Development, Winston- Salem, NC 27102. USA 9:25 2. CHARACTERIZATION OF NICOTINIC CHOLINERGIC RECEPTORS IN THE CENTRAL NERVOUS SYSTEM. Patrick M. Lippiello, Merouane Bencherif, Elisa Lovette and Kathy Fowler, R. J. Reynolds 'Ibbacco Co., Winston-Salem, NC 27102, USA 9:50 3. NICOTINE EFFECTS ON WORKING MEMORY PERFORMANCE IN THE RADIAL-ARM MAZE. E. D. Levin, Neurobehavioral Research Lab., Psychiatry Dept., Duke University Medical Center, Durham, NC 27710, USA 10:15 BREAK 10:45 4. BRAIN-WAVE EFFECTS OF CIGARETTE SMOKING IN HUMANS. Walter S. Pritchard and John H. Robinson, R. J. Reynolds Tobacco Co., Winston-Salem, NC 27102, USA .; 11:10 5. COGNITIVE AND PSYCHOMOTOR EFFECTS OF NICOTINE AND CIGARETTE SMOKING. Neil Sherwood, Human Psychopharmacology Research Unit, University of Surrey, Milfoni Hospital, Godalming GU7 IUF, United Kingdom 11:35 6. WHY PEOPLE SMOKE: STRESS-REDUCTION, COPING ENHANCEMENT AND NICOTINE. David G. Gilbert, Smoking Research Laboratory, Department of Psychology, Southern Illinois University, Carbondale, IL 62901, USA 12:00 SUMMARY AND CLOSING REMARKS: John H. Robinson 12:10 LUNCH

t I MONDAY AFTERNOON - SEPTEMBER 26, 1994 SSi414lLA Session ChaJr.• Maurice E. Snook SSZti>ZJLB Session Chair: Cheryl E. Johnson 1:40 7. PREPARATIVE CIIROMATOGRAPHIC TECHNIQUES FOR THE ISOLATION OF TOBACCO GLUCOSIDES. Susan Tafur, Margaret Core, Robert 1:40 SEMI-VOLATILE REMOVAL EFFICIENCIES OF P VARIOUS CARBON FILTERS. P. F. Clarke, M. Dunn, R. J. K. Shepherd and M.1. Taylor, Filtrona International Hale, Ronald Bassfield and Noel Einolf, Philip Morris USA, Richmond, VA 23261, USA Limited, Hertfordshire AL5 4S1, United Kingdom 2:00 8. A FAST TECIINIQUE FOR ANALYZING ORGANIC ACIDS IN TOBACCO. M. X. Wang, S. C. Moldovesnu 2:00 16. METHODOLOGY FOR TIIE EVALUATION OF CHARCOAL ADSORPTION BY VAPOR PHASE and J. H. Lauterbach, Brown & Williamson Tobacco Corp., Louisville, KY 40232 and W. W. Weeks, North Carolina Sfate University, Raleigh, NC 27695, USA ANALYSIS OF CIGARETTE SMOKE. H. Yamazaki, Y. Sakagawa and K. Iseda, Japan Tobacco Inc., Tobacco Technology Div., 1-17-7 Yokokawa, Sumida-ku, Tokyo 130, Japan 2:20 9. THE ANALYSIS OF 3-METHYLVALERIC ACID IN TOBACCO BY HPLC. Tina E. Allen and Andrew J. jv7anso, British-American Tobacco Co. Ltd., 2:20 17. MICROPLEATED MELTBLOWN FILTERS-A NEW POLYPROPYLENE PRODUCT FOR USE IN CIGARETTE FILTttA7ION. Wiliiam R. Martin. 1800D Southampton SOI 5 BTL, United Kingdom Riverwood Ln., Roswell, GA 30075, W. John G. McCulloch, The University of Tennessee, Textiles and Nonwovens Development Center (TANDEC), Knoxville, TN 37996 A J rat d M Allen d M L b ies rti . an , an a o or , a n Inc., Dawsonville, GA 30534, USA 2:40 10. CHEMICAL AND SENSORY COMPARISON OF SOME SELECTED U. S. AND INDIAN FLUE-CURED TOBACCO GRADES. K. N. Suresh, ITC Ltd., Calcutta, 2:40 C8. ADSORPTION PROPERTIES OF SURFACE- MODIFIED ACTIVATED CARBON. Chune-Ryyl Kim Korea Ginseng & Tobacco Research Institute, Dae}eon India and Willard W. Weeks, Crop Science Department, 305-345, Korea NCSU, Raleigh, NC 27695-7620, USA 3:00 BREAK 3:00 BREAK 3:30 11. CLASSIFICATION OF TOBACCO LEAVES WITH THEIR DICHLOROMETHANE EXTRACTS. Y. Shinozaki, K. Tobita. S. Suhura and Y. Tonoshige, Japan 3:30 19. DETERMINATION OF THE SELECTIVE REMOVAL OF PHENOLIC COMPOUNDS BY CIGARETTE FILTERS USING HIGH-PERFORMANCE LIQUID Tobacco Inc., Tobacco Technology Planning Dept., 1- CHROMATOGRAPHY. Steven A. Wilson, Eastman 17-7 Yokokawa, Sumida-ku, Tokyo 130, Japan Chemical Company, Kingsport, TN 37662, USA 3:50 ff. TOBACCO BLEND ANALYSIS USING DIFFUSE REFLECTANCE FOURIER TRANSFORM INFRARED (FTIR) SPECTROSCOPY. Charles Harward and Don 3:50 20. METHOD FOR DESIGNING VENTILATED FILTRATION SYSTEMS. Larry W. Renfro, Lyd1tL Saver and Daniel C. Cobb, Eastman Chemical Co., Leyden, Philip Morris USA, Richmond, VA 23261, USA Kingsport, TN 37662-5150, USA 4:I0 13. EVALUATION OF AN ELECTROCHEMICAL PHOSPHINE DETECTOR AS A UNIT OF AN 4:10 I. NICOTINE MIGRATION IN COMPONENTS OF BLENDED CIGARE'iTES. R M. Stepwgwski; Lorillard IMPROVED WAREHOUSE FUMIGATION MONITORING SYSTEM. Richard F. Dufresne, Tobacco Company, Greensboro, NC 27405, USA Lawrence H. Gains, Kenneth W. Ingold and Robert D. Stevens, Lorillard Tobacco Company, Greensboro, NC 27420-1688, USA 4:30 14. PHOSPHINE RELEASE DURING A STANDARD FUMIGATION IN TWO TOBACCO STORAGES. $,,L BENEZET Consulting, Winston-Salem Beneze NC 4:30 22. THE EFFECTS OF MOISTURE CONTENT AND TEMPERATURE ON MECHANICAL PROPERTIES OF TOBACCO LEAF LAMINA. SundrN. tSudl , , 27106, C. W. Helms, R. J. Reynolds Tobacco Co., Winston-Salem, NC 27102 and A. Hamm Jr., Flue-Cured Tobacco Stabilization Corp., Raleigh, NC 27608, USA Ganeriwala, Arup K. Basak,loseph L. Banyasz and Zane Gibbs, Philip Morris USA, Richmond, VA 23261-6583, USA 4:50 ADJOURN 4:50 ADJOURN

TUESDAY MORNING - SEPTEMBER 27, 1994 SeliIplLA Session Chair: Raymond C. Long SessiQp @ Session Chair: S. Tom Jones 8:30 23. OVEREXPRESSION OF FOREIGN GENES RELATED TO ALKALOID AND POLYAMINE METABOLISM IN TOBACCO. Sam Boaeess, Urmi Das Gupta, Art I lunt, Neat Denton and Glenn Collins, Department of Agronomy, University of Kentucky, Lexington, KY 8:30 32. TLC-MS AND TLC-MS-MS ANALYSIS OF TERPENES WITH POTENTiAL APPLICATION TO SMOKE STUDIES. N. l. lensen, T. Sumpter, S. Hassam and R. Izac, Phiiip Morris USA, Richmond, VA 23261, USA 40546, USA 8:50 24. PLANT GROWTII REGULATING ACTIVITY OF TOBACCO FLOWER PIiENOLIC ACID AMIDES 8:50 33. MEASUREMENT OF IONS IN WHOLE SMOKE. Florian R. Perini and Jimmy Ii. Bell, Lorillard Research AND RELATED COMPOUNDS. Maurice E. Snook, Center, Greensboro, NC 27405, USA Horace G. Cutler and Orestes T. Chortyk, USDA-ARS, Russell Research Center, Athens, GA 30613 and Russell L. Malmberg, Dept. of Botany, University of Georgia, Athens, GA 30602, USA 9:10 25. USE OF NICOT1Ah:I KAWAKAMII AS A TRAP CROP FOR PROTECTION AGAINST TOBACCO BUDWORM FEEDING DAMAGE ON FLUE-CURED TOBACCO. D. Michael Jackson and V. A. Sisson, 9:10 34. THE FATE OF RADIOLABELED TARTARIC ACID IN BURNING CIGARETTES. L. H. Gains and g,8, L811g, Lorillard Tobacco Company, 420 English Street, Greensboro, NC 27405, USA USDA-ARS, Crops Research Laboratory, Oxford. NC { 9:30 :50 27565 and R. F. Severson, USDA-ARS, Phytochemical Research Unit, Athens, GA 30613, USA 26. THE EFFECTS OF SUPPLEMENTAL LIGHT SOURCES ON THE PHYSICAL AND CHEMICAL CHARACTERISTICS OF GREENHOUSE GROWN BRIGIfT (COKER 319) TOBACCO. Carolyn Keene, Roger Bass, Susan Tafur and George West, Philip Morris USA, Richmond, VA 23261, USA 27. TIIE ROLE OF RADIATION AND TEMPERATURE ON TOBACCO CAROTENOIDS. Marcos P. A. Camoos and Josb R. Pereira da Silva, Souza Cruz SA, Rio de Janeiro RI 21059-900, Brazil 9:30 :50 35. SIMULTANEOUS GAS CHROMATOGRAPHIC NICOTINE/WATER ANALYSIS IN SMOKE VIA SPLIT INJECTION ON DUAL CAPILLARY COLUMNS. Lance I. Deutsch and Anna L. leffords, Hoechst Celanese Corporation, Charlotte, NC 29232, USA 36. QUANTITATION OF "TAR" COLOR WITH SPECIFIC REFERENCE TO ESTIMATING YIELDS, QUANTIFYING ETS AND THE PRODUCTION OF COLOR SCALES. W. S. (Bill)Rjckert. lack C. Robinson, Labstat Incorporated, Kitchener, Ontario N2C I L3 and Murray 1. Kaiserman, Health and Welfare Canada. Ottawa, Ontario K I A OL2, Canada 10:10 BREAK 10:10 BREAK 10:40 28. TI IE POTENTIAL FOR CURING OPTIMIZATION TI IROUGH FEED-BACK CONTROL. Subhas C. 10:40 37. MICROWAVE MOISTURE MEASUREMENT. D,L Camn, Filtrona, Milton Keynes MK14 6LY, United Mohapatra. John S. Singleton and Charles W. Suggs, Dept. of Biol. & Agric. Eng., NCSU, Raleigh, NC Kingdom and Hans-Rainer Hernnann, Tews Elektronik, Hamburg 22459, Germany 27695-7625. USA 11:00 29. CHEMICAL AND MICROBIOLOGICAL CHANGES DURING FLUE-CURING OF NK-149 TOBACCO. Jackie M. Greene and William S. Caldwell, R. J. 11:00 38. USE OF A RANDOM ACCESS ANALYZER FOR THE ANALYSES OF MAJOR TOBACCO CONSTITUENTS. V. L. Geiss, P. S. Lewis, A. B. Reynolds Tobacco Co., Winston-Salem, NC 27102, USA Canon and J. H. Lauterbach_ Brown & Williamson Tobacco Corp., Louisville, KY 40232, USA 11:20 30. POST-HARVEST TREATMENT AFFECTS ON TSNA ACCUMULATION. Lowell Bush, Harold Burton, Naewanna Dye and Nicole Nicholas, Department of Agronomy, University of Kentucky, Lexington, KY 11:20 39. CHARACTERIZATION OF TOBACCO PRODUCTS: THE ACCQ.TAG AMINO ACID ANALYSIS METHOD FOR DIFFERENTIATING BETWEEN CIGAR AND CIGARETTE TOBACCOS. Suresh RalaeatL ATF 40546-0091. USA National Laboratory Center, Rockville, MD 20850 and Christina M. Burek and James A. Saunders, USDA- BARC, Beltsville, MD 20705, USA 11:40 31. DISTRIBUTION OF TOBACCO CONSTITUENTS WITHIN THE LEAF TISSUE. 2. OXIDIZED NICOTINE DERIVATIVES. Harold R. Burton and 11:40 40. SIMPLIFIED METHOD FOR CONTROLLING HUMIDITY IN BRIGHT LEAF CURING BARN. Charles W. Suggs and Subhas C. Mohaoatra, North Xiaochen Wei, Department of Agronomy, University of Kentucky, Lexington, KY 40546-0091, USA Carolina State University, Raleigh, NC 27695-7625, USA 12:00 LUN('lI 12:00 LUNCtt

TUESDAY AFTERNOON - SEPTEMBER 27, 1994 Session A Session Chair: William S. CaldnrH 1:40 41. ABERRANT ALKALOID BIOSYNTHESIS IN TOBACCO FROM NICOTINIC ACID ANALOGS. Franklin F. Fannin, Xiaochen Wei, Lowell P. Bush and Harold R. Burton, University of Kentucky, Department of Agronomy, Lexington, KY 40546-0091, USA 2:00 42. ISOLATION AND IDENTIFICATION OF SOME SUBSTITUTED NICOTINES OBTAINED FROM NICOTIANA TADACUM BY ABERRANT BIOSYNTHESIS. Xiaochen Wei, Harold R. Burton. Lowell P. Bush and Franklin F. Fannin, Department of Agronomy, University of Kentucky, Lexington, KY 40546-0091, USA r '. 2:20 ~' THE REACTION OF NICOTINE IMINIUM IONS WITH MODEL NUCLEOPHILES. Grayland P. Dobson and William S. Caldwell, R. J. Reynolds Tobacco Co., W inston-Saiem, NC 27102, USA 2:40 44. NICOTINE AND COTININE INHIBIT THE MUTAGENICITY OF N-NITROSAMINES PRESENT IN TOBACCO SMOKE. C. Lee, C. Fulp, E. Bombick and D. Doolittle. R. J. Reynolds Tobacco Company, Research & Development, W inston-Salem, NC 27102. USA 3:00 BREAK 3:30 43. TIIE APPLICATION OF SUPERCRITICAL FLUID EXTRACTION (SFE) FOR THE DETERMINATION OF TOBACCO-SPECIFIC NITROSAMINES IN VARIOUS TOBACCO SAMPLES Ig. OPTIMIZATION OF THE METHODOLOGY. Bogdan Prokonczvk, Ming Wu, Jonathan E. Cox, Shantu Amin, Dhimant Desai and Dietrich Hoffmann, American Health Foundation, Valhalla, NY 10595, USA 3:50 4)y. EVALUATION OF PURGE AND TRAP\TIIERMAL DESORP'fION GAS Cl IROMATOGRAPHY FOR TIIE DETERMINATION OF NICOTINE AND COTININE IN AQUEOUS SOLUTIONS. Cecil E. Hi¢eins and Amy B. Dindal, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA 4:10 47. FRESH WHOLE SMOKE MUTAGENICI'fY ASSAY WITiI YG SALMONELLA STRAINS. R. S. Lake, C. L. Gaworski, E. W. Crouse and J. D. I leck. Lorillard Tobacco Co., Greensboro, NC 27420, USA 4:30 (t~. HEMOGLOBIN ANALYSIS BY ELECTROSPRAY IONIZATION MS: QUADRUPOLE vs. MAGNETIC SECTOR. Earl L. White, Mary S. Uhrig and Betsy A. Reed-Bombick, R. 1. Reynolds Tobacco Co., Winston- Salem, NC 27102, USA 4:50 ADJOURN 5:00 BUSINESS MEETING 6:30 RECEPTION 7:30 BANQUET Session B Session Chair: Claude 1. Lewls 1:40 42. CHLORINATED PESTICIDE RESIDUES IN TOBACCO PRODUCTS: ANALYSIS BASED ON SUPERCRITICAL FLUID EXTRACTION. Mifjana V. pJgQjgyjc, Jingrun Fan and Dietrich Hoffmann, American Health Foundation, Valhalla, NY 10595, USA 2:00 50. HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF NINE N-METIIYLCARBAMATES IN TOBACCO. SlGyS• Gelder, Research & Development, Rothmans International Services Ltd, Basildon, Essex, England 2:20 51. INFRARED ANALYSIS OF (t)-SERRICORNIN. Jjm L. LYons-H and R_•bert A. Fenner, Philip Morris USA, Richmand, VA 23161, USA 2:40 52. ANALYTICAL PROBLEM SOLVING IN THE TOBACCO INDUSTRY BY FOURIER TRANSFORM INFRARED SPECTROSCOPY AND ENERGY DISPERSIVE SPECTROSCOPY. R. D. Stpvens, B.1. Craven and R. M. Stepnowski, Lorillard Tobacco Company, Greensboro, NC 27405, USA 3:00 BREAK 3:30 53. THE APPLICATION OF INFRARED IMAGING TECHNIQUES TO THE STUDY OF THE IGNITION PROPENSITY OF A SMOLDERING CIGARETTE. F_Chung Wu, Lorillard Tobacco Company, Greensboro, NC 27405, USA 3:50 14. ANALYSIS OF SELECTED MAINSTREAM SMOKE COMPONENTS OF LOW IGNITION PROPENSITY CIGARETTES. K. D. Brunnemann and D. Hoii'mann, American Health Foundation, Valhalla, NY 10595 and C. 0. Gairola, University of Kentucky, Lexington, KY 40546 and B. C. Lee, U.S. Consumer Product Safety Commission, Washington, DC 20207, USA 4:10 55. TI IE DEMONSTRATION OF AIR-FLOW AS AN IMPORTANT FACTOR IN THE IGNITION PROPENSITY OF A SMOLDERING CIGARETTE. E:Chuna Wu, Lorillard Tobacco Company, Greensboro, NC 27405, USA 4:30 56. COMPUTER-ASSISTED CIGARETTE CALORIMETRY. D. Scott Passer and Claude I. Lewis, Lorillard Tobacco Company, Greensboro, NC 27405, USA 4:50 ADJOURN

WEDNESDAY MORNING - SEPTEMBER 28, 1994 Joint Session Session Chair: Charles R. Green 8:30 PHILIP MORRIS AWARDEE ADDRESS 9:10 57. SCIENTIFIC ASSESSMENT OF THE EPA REPORT ON PASSIVE SMOKING. Christopher R. E. Coggins, R. J. Reynolds Tobacco Co., Research & Development, Winston-Salem, NC 27102, USA 9:30 58. DETERMINATION OF PERSONAL EXPOSURE OF NON-SMOKERS TO ENVIRONMENTAL TOBACCO SMOKE. Roger A. Jenkins, Andi Palausky, Richard W. Counts, Michael R. Guerin, Amy B. Dindal and Charles K. Bayne, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6120, l1SA 9:50 59. NATIONAL INCIDENCE OF SMOKING AND MISCLASSIFICATION AMONG THE U.S. MARRIED FEMALE POPULATION. Michael W. Ogden, David L. Heavner, Riley A. Davis, Mitchell F. Stiles, Robert B. Hege and Walter T. Morgan, R. J. Reynolds Tobacco Co., Research & Development, Winston-Salem, NC 27102, USA 10:10 60. "CIGARETTE EQUIVALENT" SECONDHAND SMOKE EXPOSURE: ESTIMATION BASED ON VAPOR AND PARTICUI,ATE TRACERS. Patricia Martin, Michael W. Ogden. Sheila L. Cash, Katherine C. Maiolo and Walter T. Morgan, R. J. Reynolds Tobacco Co., Winston-Salem, NC 27102, USA 10:30 BREAK 10:50 61. SECONDHAND SMOKE (SHS): A MARKET CIGARETTE STUDY. Patricia Martin, David L. Iieavner, Katherine C. Maiolo, Walter T. Morgan, Paul R. Nelson, Michael W. Ogden, Charles H. Risner and Paula S. Simmons, R. J. Reynolds Tobacco Co., Winston-Salem, NC 27102, USA 11:10 62. THE EFFECT OF SMOKERS ON THE GENERATION OF ENVIRONMENTAL TOBACCO SMOKE. Paul R. Nelson, Susan P. Kelly and Fred W. Conrad, R. J. Reynolds Tobacco Company, Winston-Salem, NC 27102-1487, USA 1 1:30 63. DEPOSITION OF ENVIRONMENTAL TOBACCO SMOKE COMPONENTS IN VOLUNTEERS. John McAuEhev, John Strong and Alec Black, AEA Technology, 551 Iiarwell, Oxfordshire OXI I ORA, United Kingdom 11:50 64. VARIANCE OF ETS NICOTINE LEVELS IN A SEMI-CONTROLLED OFFICE ENVIRONMENT. HQv Bohanon, R. J. Reynolds Tobacco Co., Environmental Affairs, Winston- Salem, NC 27102, USA 12:10 65. CONSTRUCTION OF A NEW TRAIN CAR PROVIDING SATISFACTORY AIR TO BOTH SMOKERS AND NON-SMOKERS. 1. lshii, T. Asai, M. Kawasaki, T. Sakurai, T. Nishina, M. Matsukura, Y. Ishizu, Japan Tobacco Inc., 6-2, Umegaoka, Midori-ku, Yokohama, Kanagawa 227, S. Hashizume, T. Igarashi, East Japan Railway Company, 1-6-5, Marunouchi, Chiyoda-ku, Tokyo 100 and Y. Hayabe, Kinki Sharyo Co., Ltd., 3-9-60, Inada-shinmachi, Higashi-osaka 577, Japan 12:30 ADJOURN

PosHZip Code Address: Telephone: ( 48TH TCRC REGISTRATION FORM Please type or print Name: Title (DrJMrJMrsJMiss/Ms.) First Middle City State P.O. Box No. PosdZip Code prior to August 31 $150.00 after August 31 $165.00 Guest Banquet ticket(s): @ $45.00 each REGISTR.ATION FEE (one banquet ticket included in the Registration Fee) Total: Name of Guest: City Please check appropriate box(es) bebw: State Pre-Conference Reception Sunday, September 25 (6:30 - 8:30 p 0 1 will attend Guest's Address: Street U My guest will also attend Q I will not attend GUEST will attend the following complimentary events: Monday, September 26 ~ Continental Breakfast ~ Morning Tour: Replacements, Ltd. & Mother Murphy's Flavor Lab ~ Afternoon Tour: Outlet Center & other shops Tuesday, September 27 ~ Continental Breakfast ~ Tour: Doll/Minature Museum; Furniture Museum; Lunch; Fumiture Showrooms in High Point Firm/Institution: Street . last Country Country this registration fprrl~a.0 ~pft~ vyPti't to address shown below, ~nefvse a check:. ~awn on a US bank in US dollai4; or an m44n~i~af}vnal money order in US dollars, payable to:: 1No~7 to: Registration Ctimrfi 4gth TCRC' PO Box 21682 Greensboro, NC 27420y 1682 USA FAX: (910) 373-6640

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