Tobacco Documents Online

Office on Smoking and Health a 1982 DIRECTORY On-Going Research in Smoking and Health e aE~Ea~~~~ 1 j WORLD HEALTH ORGANIZATION' COLLABORATING CENTRE FOR REFERENCE ON SMOKING AND HEALTH F ~

l 0337. HUDDINGE UNIVERSIIY HOSPITAL, S-141 t6 H.ddiqe, Sw.dea. Prof aet Tltle: Case-Refereat Study of Pancreas Cancet. Prlscipal L.e.tlptor. Norell, S. E. Objective: To study the possible relationship between pancreatic cancer and previous chemical exposure and factors such as smoking and a! ,hol consumption. Metboaa or APpro.ehc A case-referent study with two referent groups haa beea designed to assess the association between chemical exposure and pancreatic cancer. Historiea of chemical exposure related to work, housing, diet, smoking, and other factors will be obtaiaed from 100 incident cases of pancreatic cancer, 100 clinical controls frnm the same hospitals, and noocliaical controls from the area population. Results to Date: The study is in a pielmunary phase. Project Datec 1980-1984. 0338. KAROLINSKA INSTIT4TfE. S-104 01 Stockholm, Swedea. SWEDISH TOBACCO COMPANY, P.O. Box 17007, S-104 62 Stoeklolas, Swedea. Project TiRle: Effects of Tobacco Smoke Constituents on Sister Chromatid Exchanges in Cultured Human Lymphocytes. Principal L.estlsatoex Zech, L.; Jansson, T.; ChrvaIl. M.;, Eazdl, C. R. Objective: To study the relative mutagenicity of selected tobacco smoke constituents using sister chr+omatid' eachanges (SCE) and the Ames test. Methods or ApPro.cl: The induction of SCE in cultured human lymphocytes by individual smoke compounds is studied using conventional banding and fluorescence techniques. Results to Dateo Three compounds in a series of substituted methylindoles (2,3- dimethylindole, 2,3,1-trimetbylindok, and' 2,3,b-tsimethylin- dok) which have previously been shown to be mutagenic by the Ames test have been found to eauae SCE. Project D.t,ea: 1979-continuing. Fl.a.d.l S.Nort: Karolinaka lnstitute; Swedish Tobacco Cempany. 0.'139. >YAROLIIVStA IIVS'1TRTM Deprde.e .[ Hi.tol- op, Box 60100, S-104 01 StodtLol., Sw.dea. Project Tltle: Nicotine, Catecholaminn and Nettropeptides and Neuroen- docrine Functions. Pri.dpa1 la.estisatoe: Fuxe, K. Objective: To (1) analyze the actions of acvte and chronic nicotine treatment and exposure to cigarette smoke on the various hypothalamic catecholamine nerve terminal Bystems of rats in various endocrine states; k2) relate the actions on these systems to effects on the secretion of luteiniring hormone (LH), follicle- stimulating hormone (FSH), prolactin, adnaooortimtropic hormone (ACTH), corticosterone, growth hormone, thyroid- stimulati.n8 hormone (TSH), and vaaopresaia;,and (3) study, by means of quantitative immunocytocDemistry, the possible direct effects of chronic niootine and exposure to cigarette smoke on LFIItJi, somatoatatia, and' TRH immunoreactive nerve terminals of the median eminence to discover possible signs of aetions on peptidergic cells. Metloda or Appeoaeh: Biochemically regional catecholamine and SHT stores are analyzed by amine fluorescence histochemistry, quantitative immunocytachemistry, aod hi8'b pressure liquid chnomatogn- phy with electrochemical detection. and radioimmuao.asays of hypophyaeaW hormones are made. Ranlts to D.ek The results show that acute exposure to cigarette smoke or niootine injections produce widespread activations of hypotha- lamic dopamiae and noradrenaline n.erve terminal systems which probably are produced' via activation of nicounic cholinergic reoepton controlling the synthesis in the release of catecholamina in the various parts of the hypothalamus. Furthermore, nicotine in repeated doses prod'ucea lowering of LH, FSH, and prolactin secretion. effects which seem to be at least in part mediatad via activation of the tubervinfundibular dopamine neuronss. Nicotine can produce reductions of discrete hypothalamic noradrmalinc levels within a few minutes and can also markedly eahanee the secretion of ACTH and vaaopra.rin, actions which also may involve effects on hypo- thaLmic noradrenergic mechanisms in discrete areas. Fndve Pfa.a: Studies on chronic exposure to cigarette smoke are planned so that the effects of smoking ia a chronic situation can be compared with chronic exposure to niootine. Project D.t.r January 1982-1983. Pta.eei.l S.„ort: CTR /0. , LAROLINSICA INSi1TCITE, Dep.rtaeat of T.asor Biolop, S-104 01 Stoekiolr, Swadea. Project Tft1c Devebpmeat of an In Vitro Screening Test for Tumor Promoters in Tobacco Smoke. Pfincipal loventlestors: Klein. O.;1Callin, B. Objective: To design a good 'm vitro screening procedure for detection of tumor prvmoten. Metlob or ANru.el A test systeatm, baaed on the ability of tumor promoters to induce viral antigen syntbesis in Epatein Barr Virus carrying Burkitt lympboma cells when combined with n-butyrate, is being uaed The presence of a synergistic eNect between n- butyrste and tumor-promoting phorbol eatas, resulting in an early antigen induction, has been shown to correlate extremely well with the tumor-promoting activity of the rsspective phorbol eater in miee. Fractions of tob.ooo smoke are currently being tested for tumor promoters. Results to IDate: The study is in a preliminary phase. Project Dates January 1982-1984: F1..aeial S.gyort Swedish Tobacco Company (8125} Swedish Work Environ- ment Fund. 109 zV