Biochemical Modification of Tobacco 810701 - 810731
Date: 07 Aug 1981
Length: 2 pages
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Length: 2 pages
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- Teng, D.M.
- REPT, REPORT, OTHER
- BIBL, BIBLIOGRAPHY
- OUTL, OUTLINE
- CENTRAL FILES/DATABASE
- Named Organization
- Lancaster Leaf Tobacco
- Named Person
- Mooz, E.
- Morgan, R.
- Nixon, G.
- Semp, B.
- Teng, D.M.
- Tenhet, S.
- Walls, K.
- Master ID
- 2022151336-1407 Monthly Progress Reports Period Covered 810701 - 810731
- 2022151339-1340 Analytical Research
- 2022151341 Mechanism for Smoke Formation 810701 - 810731
- 2022151342-1343 Cigarette and Tobacco Measurement Methods 810700
- 2022151344-1345 Improved Semiworks Operations 810700
- 2022151346-1347 Entomological Research 810701 - 810731
- 2022151348-1350 Reconstituted Tobacco Development 810709 - 810806
- 2022151351-1352 Modified Smoking Materials 810701 - 810731
- 2022151353-1355 Smoker Psychology 810701 - 810731
- 2022151356 Filtration Physics 810701 - 810731
- 2022151357-1358 Cigarette Making Technology 810701 - 810731
- 2022151359-1360 Tobacco Physics 810701 - 810731
- 2022151361-1362 Physical and Chemical Properties of Tobacco 870701 - 870731
- 2022151363-1364 Tobacco Microstructure 810701 - 810731
- 2022151365-1367 Tobacco Processing 810701 - 810731
- 2022151368-1369 Expanded Tobacco - Process Improvement 810701 - 810731
- 2022151372-1373 Microbial Technology 810701 - 810731
- 2022151374-1375 New Products 810700
- 2022151376 Filter Development 810700
- 2022151377 Applied Technology 810701 - 810731
- 2022151378 Flavor Development 810701 - 810731
- 2022151379-1380 Flavor Development 810701 - 810731
- 2022151381 Flavor Component Evaluation 810701 - 810731
- 2022151382-1383 Synthesis of Tobacco Additives 810701 - 810731
- 2022151384-1386 Nuclear and Radiochemistry of Smoke 810701 - 810731
- 2022151387-1388 Botanical Investigations 810701 - 810731
- 2022151389-1390 Flavor - Release Chemistry 810701 - 810731
- 2022151391-1393 Synthesis of Flavorants 810700
- 2022151394-1395 Chemistry and Isolation of Tobacco Constituents 810701 - 810731
- 2022151396-1397 Smoke Studies 810700
- 2022151398-1399 Brand Modifications 810700
- 2022151400-1402 Biological Effects of Smoke 810701 - 810731
- 2022151403-1406 Smoke Condensate Studies 810701 - 810731
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CHARGE NUMBER: 1901 PROJECT TITLE: Biochemical Modification of Tobacco PERIOD COVERED: July 1-31, 1!981 WRITTEN' BY: D. M. Teng DATE OF REPORT: August 7, 1981 I. LANCASTER LEAF TOBACCO COMPANY (LLTC) - TYPE FERMENTATION OF . Teng, , emp, S. en et A large scale solid fermentation of Burley strips and'stems was conducted at LLTC during June and July of 1981. Burley strilps and stems were sprayed with either water or sugar solution to desired moisture content, incubated at thermophilic temperature range for up to three weeks and'dried. Samples were analyzed at R&D, and the results indicated that: . Under certain conditions a very significant reduction in nitrate-nitrogen was observed in Burley strips in two weeks. b. The large scale sample took longer for the reduction of nitrate as compared to a small scale sample under the same condition. Different types of containers will be used next time. c. Different batches of tobacco responded differently to the application of sugar to the tobacco. d. The required conditions for this type of fermentation can be achieved at LLTC. Small scale Burley.strip experiments were carried out at R&D to determine the effect of inital pH, sugar concentrations and moisture content to the degree of nitrate removal by solid fermentation. Results will be reported upon the completion of the experiments. (D. Teng and S. Tenhet)2 II. FARM STUDY (D. Teng, G. Nixon, K. Walls)3 Coker 319 Bright tobacco leaves were primed and flue cured at the Southern Piedmont Experiment Station at Blackstone, Virginia. Samples were taken at different stages and freeze drie6to be used for chemical constituents, especially pectin, determination in the future. Coker 319 Bright tobacco plants were also stalk cut and are being air cured. Tobacco leaves subjected to flue curing and air curing willl be used in a study to determine the effect of sugar to filling power and expansion.
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IT , I -2- III. TOBACCO PECTINMETHYLESTERASE (E. Mooz, G. Nixon, K. Walls, D. Teng)4 The optimum condition for isolating pectinmethylesterase from green and flue-cured bright tobacco leaves was established. The enzyme preparation is stable to freezing, but not freeze drying. Coker 319 tobacco samples were taken at different stages of flue curing, and the pectinmethylesterase activity was determined. On dry tobacco weight basis, the enzyme activity showed a steady decline as the curing progressed'. The enzyme profile in tobacco during air curing also willi be determined. IV. TOBACCO EXPANSION A. A small scale expansion process was used to test the effect of stiffening (increase in C.V.) from an increase in expansion (increase in S.V.). Bright filler at various levels of moisture and Burley filler at 40% O.V. were heated for various times by the radiant heat system, and equilibrated in the laboratory. From the S.V. values, it was concluded that the increase in C.V. by radiant heating was due to stiffening than expansion. (~E. Mooz, K. Walls, G. Nixon)5 B. Fourteen samples were submitted to Dr. R. Morgan for Ca determination. They consisted of bright filler and cylinder-treated bright filler which had been expanded without puffing agegt in the 3" tower at temperatures of 675°, 650° and 600°F. (E. Mooz) REFERENCES 1. Di. Teng - PM Notebook #7418. 2. S. Tenhet - PM Notebook #7225. 3. G. Nixon - PM Notebook #7208. 4. K. Walls - PM Notebook #7528. 5. E. Mooz - PM Notebook #7443'. ~ O N N N U1 ~ h+ 4'