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CHARGE NUMBER: 1101 PROGRAM TITLE: ENTOMOLOGICAL RESEARCH PERIOD COVERED: July 1-31, 1981 PROJECT LEADER: M. A. Manzelli DATE OF REPORT: August 5, 1981 I. INSECT GROWTH REGULATOR The Maury Street Warehouse/KABAT(D field test continues to demonstrate low adulit cigarette beetle populations within the treated warehouses as compared to the untreated controls. In addition, adhesive traps in the exterior of the treated warehouses generally show higher counts than the inside traps, indicating that adult beetles are flying into the treated ones.1 II. CIGARETTE BEETLE PHYSIOLOGICAL STUDIES A. Sex Pheromone Studies Laboratory studies with three different batches of synthesized sex pheromone have shown them to be of equal effectiveness at concentrations above 10-4ug. Fie1d'studies have been initiated with the pheromone.2 B. Larva Symbiont Studies By means of the electron microscope, it has been shown that the symbionts isolated from beetle eggs in the laboratory appear to be identical to descriptions previously given in the literature. The symbionts have been cultured in agar and in a nutrient broth.3'4 III. ASSISTANCE TO OTHERS A. Expanded Tobacco--Insect Spotting The CO2 tobacco expansion process appears to reduce the capability of any insect body or part to spot cigarette paper during the manufacturing process.5 B. Louisville Warehouses A survey, followed by recommendations, was made for a heavy beetle infestati'.on in warehouse number 30.6

Charge Number: 1101 2 August 5, 1981: C. Chesapeake Warehouses The effectiveness of manual fogging in open versus closed' warehouses with DDVP against the beetle was studied. Over 99% control was achieved in the closed warehouse, whi!le only 66% was obtained in the open one.7 D. Alleghany Export Warehouses A similar test at Al,leghany gave greater than 99% control in a cliosed warehouse with manually applied DDVP versus only 17.5% control with the automatic DDVP applicator system.8 IV. REFERENCES 1. Minor, M. F. Notebook No. 71,97, p. 107. 2. Long, J. S. Notebook No. 7283, pp. 167-8. 3. Lehman, R. M. Notebook No. 7235, pp. 140-1. 4. Lehman, R. M. Memo from Vicki Baliga. 1981 July 13. 5. Lehman, R. M. Notebook No. 7283, pp. 166-7. 6. Manzelli, M. A. Memo to W. F. Johnson. 1981 July 24. 7. Lehman, R. M. Memo to M. A. Manzelli. 1981 July 27. 8. Long, J. S. Notebook No. 7283, pp. 164-6. ;W&?. W44~T~* nwp