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. 40CORPO.RATE WOODS.,SUITE 650 94O11INDIAN CREEK,PARKWAYP.-0. BOX 2S128OVERLAND PARK. KANSAS 66225 (913) .451F6060 TELECOPIER 19131~45I~6O60 1K149 ZAP MAIL /9131 45.1-2795 SHOOK, HARDY & BACON TWENTIETH FLOOR MERCANTILE BANK TOWER. 1101 WALNUT KANSAS CITY, MISSOUR1164106 (816) 474-6550 TELECOPIER .(8116) .421-5547 W.U.T..TELEX 426291 (6HB KSGZAP MAIL 18161.474-6551 May 8, 1986 LAW OFFICES Messrs. Alexander Holtzman Wayne W. Juchatz Josiah S. Murray, III Ernest Pepples Paul A. Randour Arthur J. Stevens Re: A. Bennett Jenson, M.D. Gentlemen: TWELVE WYANDOTTE PLAZA 120W£ST 12th STREET, SUITE 600 KANSAS CITY, MISSOURI 64105 (8761474-6888 TELECOPIER (816) .47.2-7069W.U.T.,TELEX 424025 (SHK HDYBAC UD) ZAP MAIL (816) 474-4064 Dr. A. Bennett Jenson of the Department of Pathology, Georgetown University Schools of Medicine and Dentistry has sub- mlitted a proposal to continue his research on human papillomaviruses and squamous cell lesions of the respiratory tract. He has been supported'by a CTR Special Project for several years. Funding is requested' for a two year period withl $84,957.001 and $88,170.00 for the first and second years, respectively. Enclosed is a copy of the new research proposal. Dr. Jenson's current work in this area is focused onthe identification of antigens of the human papillomaviruses (HPV) using peroxidase-antiperoxidase techniques. He has reported positive reactions or the presence of HPV antigens in lesions from the oral cavity and respiratory tract. However, not all lesions are associated with HPV express viral antigens, according to Dr. Jenson, and he is interested in investigating thlis associ- ation using molecular DNA techniques. N O !-~ Detection of HPV DNA sequences in these lesions is gen- ~ erally accepted as the best virologic evidence of identifying HPV O as a possible etiologic agent. In addition, new techniques have ~ been developed~which will permit detection of viral DNA sequences informalin-fixed tissue whereas older methods were restricted to freshlor fresh-frozen tissue. This technical advance will enable Dr. Jenson to analyze retrospectively archival collections of formalin-fixed tissue. This technique, called in-situ hybridiza- tion, would be applied to specimens of lung and other tissue selected from the files of Baptist Memorial Hospital in Memphis, Tennessee and the Armed Forces Institute of Pathology in Washington, D.C.

\ ' SHO9K, HARDY & BACON Messrs. Alexander Holtzman Wayne W. Juchatz Josiah S. Murray, III Ernest Pepples Paul A. Randour Arthur J. Stevens May 8, 1986 Page 2 Dr. Jenson believes this work is important in his con- tinuing study of the role of special viruses in the development of squamous cell carcinomas. In this regard, Dr. Jenson has been extremely productive, as evidenced by the attached list of publi- cations produced under past CTR support. Dr. Sommers of CTR has reviewed Dr. Jenson's research proposal and recommends funding. We also recommend approval of the proposals as a CTR Special Project. ience. I would appreciate a response at your earliest conven- PMS/ts Enclosures cc: Francis K. Decker, Jr., Esquire (w/encl.) Janet Brown, Esquire (w/encl.) Michael A. Nims, Esquire (w/encl.)