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of Pulmpnary Adenomatosis in D~BAf2 Mice by the Oral Administration of 'Comnents on Abstract by K. C. Snell and H. L. Stewart entitled., "Induction ,:. ,:. . . . :r+3- ..n::,;x .. . . . , ... . _.., . . Dibenz(a,h)anthraeene," published in Authors' Abstracts, Eighth Inter- ~: national Cancer Congress,.Mascow, U.S.S.R., July 22-28, 1962: ,•~ s T t`Y` ~~: The attached abstract shows that for a relatively sma]l group of . .,:.~rn,,,;, , ~ ... ~.~::. ~.•,. r::;: ' 5 27 mice who were fed an aqueous olive oil emulsion of enz bi~ anthracene, r~tr an unstated number of animsls developed alveologenic carcinoma of the {~~La' L! . 4~~' ~.Y.,~ a r:~.,. _ i fi4, ~. ~ ~+~ :y.'y.., -'f~ - . , . •• •+ '~ i lung which Dr. Steward .~.. also called pulmonary a denoma . ~ •^ tosis. In contrast 41! to the abstract by Fylev, this lesion is not one which would be con- `'~~~y p2 ,N ~ f~~PfiSr. sidered to be strictly comparable with the human carcinomaof the lung Mf7~s~;~ ~ f • said to be associated with smoking. • /rR Attachment: copy of abstract ::-..'.~ .,, ~~. - • .,. . - ~,.. - . . ~.... :..'... ,.,... ~ .. ~..~ .. :~;:~~ u .:.'~.•~ V5 -'i.ntls: <.~ wM1~ ! f•, :-i rK.

p, ..: •.. . . . .4.::..... •': i.;°.' r?•:'= '= ;-e~-•e... ~ ~~Y. ~ +~- ~ ~ es Ir~! ,~. . I NDUCTION OF LUNG CANCER IN RATS BY INTRATRACHENAL INSUFLATION OF CANCEROGENIC HYDROCARBONS .„_. ... L. N. PYLEV USSR, MOSCOW ; 9,10-dimethyl-1,2-benzanthracene (DMBA) was inted to rats intratracheally a:- by intubation; Wistar and noninbred rats were used. In some groups one intubation i; pf 2.5 mg DMBA was performed, in other groups DMBA was introduced three or "five times (2mg per intubation; the total dose amounting respectively to 6 and 10 mg), •"'~ In other experiments 3,4-benzpyrene was introduced in.the doses 5 mg five and seven z•~ n. times (that is, in the total doses 25 and 35 mg). The interval between injections was •' ;"about a'month. Cancerogen was added to the colloid protein solution containing small amounts of Indian ink. Lung cancer appeared approximately in one-third of the animals which have survived more than 5 months from the beginning of the experiment. Most of the lung tumours were histologically squamous carcinomas; but there were several cases of adenocarcinoma. Both types of neoplasms gave methastases in peribronchial, para- thracheal and paraverthebral lymph nodes. Tumour development was preceded by changes, which one may characterize as L ~ precancerous: focal metaplasia of bronchial epitheliums, papillomas and polyps of bronchial epitheliums, adenomas and papillomas. - Thus, the possibility of the systematic induction of experimental lung cancer in rats was shown. 13 '? -.n. . . .. - _ ~..,. , ... .. .. - . ... .- . . . ',,J,'~~6~. - i. . .~:~ :.- . K. C.- S NE L L and H. L. S T E W A R T USA, BETHESDA , 1 NDUCTION OF PULMONARY ADENOMATOSIS IN DBA/2 MICE BY THE ORAL ADMINISTRATION OF DIBENZ(A,H)- ---•-•--_ . ..-._.~ . All of 14 male and 13 female DBA/2 mice that were given, in place of drinking water, an aqueous olive oil emulsion of dibenz (a,h) anthracene for 200 days or longer, developed pulmonary adenomatosis. The lesion was found also in one control! mouse given the olive oil emulsion without dibenz (a,h) anthracene. Mice that drank the emulsion containing dibenz (a,h) anthracene developed alveologenic carcinoma, tumours of the mammary gland, precancerous lesions of the small intestine and haemangio- endotheliomas involving the pancreas, mesentery, and abdominal lymphnodes. These and other lesions induced by the treatment, as well as spontaneous lesions to which DBA/2 mice are subjects, will be described and discussed. INFLUENCE OF SOME NUTRITIONAL FACTORS ON URE- THANE-INDUCED PULMONARY ADENOMAS IN MICE F. A. FRENCH, . ., A. •H O S K I N G and J. F R E N C H USA. SAN FRANCISCO Previously we have reported that high but well tolerated doses of nicotinamide, given orally, significantly reduce the number of pulmonary adenomas in urethane treated strain A mice. These effects were observed with mice at different age levels, from different sources, on diverse basic diets and with a variety of dosage schedules. Deprivation of exogenous niacin erratically increases the incidence of pulmonary adenomas. Subsequently It has been found- that high levels of nicotinic acid have no effect on the tumour incidence. In experiments where nicotinamide was given before overlapping and after urethane treatment the only significant result was that the antitumour effect is roughly proportional to the length of treatment after the initiation of carcinogenesis. Choline does not negate the antitumour effect of nicotinamide and hence the observed effects are not due to methyl group deprivation. Also we have found, in independent experiments, that choline in the form of its citrate and tartrate salts significantly decreases the number of tumours. Choline bitar- trate gave 7.60 ± 0.57 and controls 10.05 ± 0.59 tumours per mouse (groups of 40 mice), p - 0.0039. Choline dihydrogen citrate gave 3.60 t 0.32 and controls 5.86 t t 0.42, p 0.0001. Preliminary experiments indicate that Inositol is also an active . , . 12-373 IT7 ` ,a ,e..i ~ ~ , `~' i ~ , F .•i~ ~~.y ,r '~ ~ ~ i ~'.;. -~ ~'~ C ,y ~ p• t :i,~ .:'~`j.~j.Y -•~Yl'~C Y. ..~{ r .s~,~ ?~~3, ' (f ~~~-•~~ .r. ~_-~,•_ `~'~`• E.? ~! 4.-°:: : ~„~:• +; ;V' `_. ~,. + ,-_ • c..,.,