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VIRUS, Previous Infection DEPARTMENT OF PATHOLOGY HARVARD MED1Cr*_ SCHOOL s w a...+r....+r e+er.. . ^ Mr. W. T. Hoyt, Executive Director September 6, 1967 -. BOBTON. MASS: 02115 ................................... The Council for Tobacco Research - U.S.A. 633 Third Avenue, New York, N. Y: 10017 .Dear Mr. Hoyt: I am writing pursuant to my telephone conversation and with a high degree of efficiency. .certain of our investigations. I believe a modest increase in ,our budget will make it possible to conduct our studies productively the grant entitled "'Biology and Cytopathic Effects of Respi.ratory and Oncogenic*Viruses in Organ Cultures of Human Respiratory Tract Tissue." As indicated in my report of June 30, 1967, the work supported by this grant has progressed most satisfactorily although costs have been greater than initially anticipated. This is in part due to the success of our initial studies but more because increases in supply costs and'salaries have been substantial during 1967. In addition we have found it necessary to utilize commercially supplied cell cultures in order to conduct with your staff member regarding a revision of the budget for Enclosed please find a revised budget for the 3 years h of grant support. You will note that the budget for the present first year has been increased by $2,572. This will compensate for a wage adjustment for non-professional personnel made by the University on July 1, 1967, increased demands on our part- time histologist, and the use of commercially supplied primary human cell cultures. Proportional increases have been made in the revised budget for the second and third year. These changes anticipate the growing scope of our work and increased costs. I trust the Progress Report'submitted to your office on January 30, 1967 will provide detailed information on our work. Should a more comprehensive up-to-date evaluation of these studies be required, please feel free to contact me. I would be happy to provide specific details on matters of salaries and supply costs. Sincerely yours, „ f 1 0 ~ © GJ ~ ,cfohn E. Craighead, M!!(D ~ssistant Professor t

REVISED BUDGET Biology and cytopathic Effects of Respiratory and Oncogenic Viruses in Organ Cultutes of Humin Respiratory Tract Tissue A Principal Investigator: J. E. Craighead Principal Investigat br. Retirement, Social Security, - etc. 16% Technician (full time Histologist (shared) Secretary, bookkeeper, dishwasher, (shared) 9 1/4% Supplies Media, serum, chemical Commercial-supplied cell cultures Glassware Disposable plastics Histology supplies Paper, secretarial supplies Miscellaneous Equipment Replacement parts and service Year 1 2790 446 5175 1500 1600 765 5800 2000 1800 888 1200 1250 1300 1500 2000 2200 500 500 500 500 550 600 200 200 200 100 100 100 300 300 ~ 300