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?3 DAUGHTER CELLS FOREIGN DNA is,splieed into the pSC101 plasmid and introduced scith thr plas mid into the bacterium Esche i hia li Th l id y_ r c co . e p asm ir,cleaved by the endonuclease Eco RI at a single site that does not i intrrfr . ith h la id' f l , re c t e p -m s genes or rep ication or for resistance „z~,,.. .. t . Y o tetrae cline Itop leJt l, The nucleotide sequence recognized by Eco RI is prerent also in other DN a, so that a foreign DNA ex- posed tolthe endonuclease is cleav'ed about once in every 4,000 to ~~vA,: 16,000 nucleotide pairs on a random basis (top right). Fragments of cleaved foreign DNA are annealed to the plasmid DNA by hydro- gen bonding of the complementary base pairs, and the new com• posite molecules are sealed by DNA ligase. The DNA chimeras, each consisting of the entire plasmid and a foreign DNA fragment, are introduced into E. coli by transformation, and the foreign DNA is replicated by virtue of the replication functions of the plasmid.

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