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Budget J~'fification (contim~) 1) SDS protein gel elecu~hore~ appara~ i~ needed for fractionation- Bio Rad Inc. 2) Ball bearing cell lmmeSeaiz~ is ~ designed for h/gh efficiency and high reproducib'dity of cell homo~nization for tisme culture cel~ that minimizes organelle dama~ Ham W. h~e~, H& Y Enterprtu~.

Andrea O. Sant, Principal Investigator P.LNam~ Andrea J. 5an~ 10% 5,800 Te~lmicai S~pBort Jennifer Laiprasert All Fringe Benefits 80% 18,000 5,831 A. Sdm~s Sutneud 29,631 B. Co~lesal~|ies~ym~orc=tegory) i) Tissue culture media, media, FCS, supplements 5,000 2) Tissue culture plasticware 5,500 3) Selection drugs for transfection 2,500 4) Protein biochemistry supply 3,500 5) Radioactive isotopes, immunofluorescence supplies 4,500 B. ConsaraablesSub~tal. 21,O00 Oth~Expeases(i~mize) ~) Radiation protection and waste disposal 3,500 7) Mice purchase-75 mice/year @ $15/mouse + I0% set-up fee 1,238 Mice-care 10 cages @ $.75/cage/day 2,737 8) Travel-1 mtg, per year 1,000 c. Oth~F~peoses Subto~d .... 10~475 9) Publication costs/photo 2,000 A+B+C Sub~tal 6!+!06 D. 1NDI'RJ~CT COSTS ( 15% of A + B + C). D. Iodi~e~Co~-~ 9~166 SDS protein gel electrophoresis apparatus 4 sets of extra plates Ball bearing cell homogenizer (6) stainless steel ball bearings - i ,095 2OO I, 260 180 E. Penn~ntEq~i._pra~Subtot~t .. 2.~73.5 . I~cate hem a~d on Fage I. F .................................................................................................... ......... TOTAL REQUEST 73,007 64,161 73,785 Year3 67,~ - O* - lO, lOS :]~ ,474

T~le of Prok~ Cell Lineage Dependent Anti- gen Presentation Nolecular Control of MltC Clas.' l! Restricted Antigen Presen- tation Regulation of HHC Class [I [ntrace] I ul ar Transport cost 1 year extension I~u~y aad de~c~be 27 o-P~t~p of this NIH R03 RR08896-1 Arthri ti s FoundaLior NIH RO1 AI-34359 To~ Va~e o~ (dU~ co~) $24,623 $135,000 $475,848 $24,623 $45,000" $100,873 None I~ of 11/30/94 6/30/94* 01131/98 ~udicate the ~ot~t mm~al funds available to you th~s year for all ~e~.ar~ ch projecm • ~der your sup~vision. 170,496 PENDING OR PLANNED MHC ClassII Restricted Presentation of Endogenous Antigens TuBor Antigen P~esentation by Class II Holecules NIH R01 EM51629-01 $751,456 $286,149 (give gram of G~ Amoum a~t~.~) (direct costs) Av~lable to You $t38,423 ACS $93,810 To~ Du~-ation -- 07/01/94- 06/30199 07101/94 - 06/30196