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PFII~CtPAL INVESTIGATOR/?I~OGRA-.1 DIRECTOR; E]ias Bal binder BIOGRAPHICAL SKETCH G~ve the follov,~ng information for key professional personnel listed on pa§e 2, b~nning with the Principal Investlgator/Pro~ram Director. Photocopy this pa~ for each person. NAME . ITITLE IBIRTHDATE(~o..Da~.Yr., El ias Balbinder I Adjunct Pro+essor I 01/22/2/= ! EDUCATION/Be~n w]th bacca!au~ate or other initial profe=sfona! educatiGn and include postdoctoral troining) INSTITUTION AND LOCATION Jniu. of Michigan~ Ann Arbor, HI Indiana Uniu.~ Bloomington~ IN Cargegie inst., Cold ~Pg. Harb.~ NY ~niv. of Calif.~ San Diego, CA DEGREE highest degree/ E~.S. Ph.D. Postdoc Postdoc YEAR CONFERRED 19~9 1957 1957-~0 19&0-~3 FIELD OF STUDY Zoology Zoology (Genetic ) Bacterial Genetics Biochemistry RESEARCH AND/OR PROFESSIONAL EXPERIENCE: Concluding with present position, list in chronological or(Jet previous employment, experio enceo and honors. Include present membership on any Federal Government Public Advisor~ Committee. List, in chronological order, the titles and complete refecence~ to all publ|cation$ during the past three years and to representative earlier publications pertinent to this application. DO NOT EXCEED TWO PAGES. 1963-67 Assistant Professor of Genetics, Dept. of Bacteriology and Botany~ Syracuse University~ Syracuse~ NY. 1967-71 Associate Professor o÷ Genetics, Oe~t. o÷ BacterioloBy and Botany, Syracuse University, Syracuse, NY. 197i-76 Professor of Genetics~ Dept. of Biology, Syracuse University, Syracuse, NY. 197&-82 Head, Laboratory of Genetics and Carclnogensis~ AMC Cancer Research Center and Hospital~ Lakeuood~ Colorado. 1982- Adjunct Prmfessor~ Dept. o~ BiochemlBiophys/Genetics~ University of Colorado Health Sciences Center~ Oenver~ Colorado ~mards~ Fel Iouships, etc. 19&O-&3 Postdoctoral Research Fello~ American Cancer Society - Laboratory of Or. David M. Bonner~ University of California, LaJolla, California. 2/&3 to Fulbright-Hays Auard to conduct research at the Department of 8/63 Biology, Univ.of Buenos Aires~ Argentina. 2/7D to Special Fello~ship of the National Institute of Health. To con- duct research in the laboratory o÷ Dr. Aizo Matsushiro, Osaka University, Osaka, Japan. 1977-79 Member, National Science Foundation Panel on Genetic Biology. 6/~ to Recipient of Fulbri~ht-Hay5 Auard and Visitin9 Profe~sor~ Univ. of Los Andes, Bogota~ Colombia. To teach course in Molecular Genetics. SPlected Publications 1. LaScolea~ L.J., and Balbinder, E. Restoration of phosphoribosyltrans- ferase activity by partially deletin9 the trpB 9ene of the tryp- tophan operon of ~.typhimurium. J. Boot. 1~___~2= 877-885 (1972). 2. Macrina, F.L., Balbinder: E., and Bassel, Alix, Molecular charact- erization of a stable F~lac plasmid. Biochem. & Biophys. Res. Comm. 54= 737-7~3 (1973). 3. DiCam~lli~ Ralph and Balbinder~ E. The association of tryptophan synthetase subunits from E. coil and S. typhimurium in homolo- gous and heterologous combinations. Genetics Res., Camb. 27: 323-333 (I97&). 40000221

" PR~X~;," ~',~-~TO~,~RC~.R~;~ C~RZ~TO~ El ias Bal binder ~. Callaharl~ R. III, Dooley,M.M., and Balbinder~ E. A mutation to methyltryptophan dependence in the tryptophan (trp) operon of Salnonella typhinurium. If. S±udies of 5-methyltryptophan-depen- dent mutants and their revertants. Holec. Gen. Genet. 5. LaScolea~ L.J.~ Oooley, H.H.~ Tor9et, R., and 8al6inder~ E. A muta- tion to 5-methyltryptophan dependence in the tr~ operon of Salmonella typhJmurium. III. Correlation 6etween phenotype and the proper- ties of the second enzyme ÷or tryptophan biosynthesis in a 5- methyltryptophan dependent mutant and. several 5-methyltrypto- phan-Jndependent revertants. Molec. Gen. Genet. 1~___~5: 1~5-~53 (197~). &. Angelosanto~ F.A., Torget~R., and Balbinder,E. A mutation to a methyltryptophan dependence in the ~ operon o÷ Salmonella muriu~. IV. isolation and c6aracterization o~ t_Z_p- promoter muta- tlons. Holec. Gen. Genet. ~6~: 255-~6& (19~). 7. Dooley~ M. and Balhinder~ E. Di+ferences ~et~een the AntNranilate- 5-pNospNori~osyl ~yropNospate pNospNoribosyltransferases o+ Salmonella typ.Nimurium straihs LT~ and LTT. J.Gen. ~. Balhinder~ E.~ Reich, C.I.~ SNuBarts~D.~ KeoB6~ J.~ Fi~iBer~ Jones~ T.~ and Banks~ A. Relative muta9enlcity o~ some urinary meta~olites o~ the antitumor drub cyclopNospNamide. Cancer search ~: 2967-2972 (1981). ~. Balbin~er~ E.~ ~erry, D. ana ReicK~ C.I. Deletion inauction in ~ac- ~e~ia. ~. TKe ro~e o~ m~tagens ana ~ellu~ar erro~-prone Mut. Res. I12: i~7-168 (1983). iO Ba;bin~er~ E~Stlck~ S.N. and Sharma~ O.K Complex e~ects o~ t~no; on the metabolic activation o~ 2-am~no~luorene. Env. Mul. ~: 665-67~ 1I~ Balbinder~ E. and Kerry D. A new slrain o~ Salmone))a Iyphymurlum reverted by mJiomycin C and N-methyl-N-nitro-N-nitrosoguanidlne; a eossible universal tester ~or mulagenic compounds. Nut. Res. 315-320 12. Balbinder~E. Use o~ pre-designed plasmJds to study ~eletions: strelegies ~or dea~in9 mJt6 a compJex prob~em. CKa~ter ~O.In DNA Rep.~icatlon and Mutagenesls.Eds.R.E.Moses and W.C.Summers (in ~ress~igBS). 13. BalbJnder~E.~MacVean~C. and Wi I I iams,R.E. Deletions on special ~y designe~ ~erivatives o~ plasmi~ pBR325 in E.coli. Mut. Res. (in ~ress~lgB8). ~. B~binder~E. Oelelion enBancemenl in slrains constilutiue tKe SOS response. <in preparation>. 40000222

Estimated duration of different phases of project Ist. Year (a) Studies on effect of asymmetric duplications of terminal repeats (objective 1). (b)Attempt to recover deletion intermediates (objective 4). 2nd. Year (a)Studies untargeted?. (b)Continue studies above as needed. 3d. Year (a)Participation of RecA-mediated production of deletions. (b)Continue studies above as needed. of mutagen-induced deletions:are they recombination in Budqet-EstSmated direct costs for first vear. Personnel Dr.E.Balbinder (P.I.,70%) ....... $43,000 Technician ..................... $20,000 Total salaries ................... $63,000 Supplies add miscellaneou~ Consumables(culture media, disposables, enzymes,chemicals)..$ 8,000 Miscellaneous(publication costs, telephone, mail,copies) .......... $ 2,000 Total supplies &misc ........... $10,000 Total Direct Costs ............... $73,000 40000223