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BIOGRAPHICAL SKETCH Give the following] ~nformat~on for key professional personn~| listed on pa.~ 2, b~jinn~n;; with Principa! Investigator/Pro;Faro Director. Photocop,t this pa;p for each person. NAME ITITLE El ias Ba'lbinde~ AdJunct Pro÷essor EDUCATION ~Beg~n w~th baccalaureate ~ other inlt~a! profe$$f~nal education and include po~tdoctofa/ training/ 81RTHDATE/Mo.. Oai,o Yr.) 13:1/22/25 DEGREE fc#cte YEAR FIELD OF STUDY INSTITUTION AND LOCATION hi~hesr degree) CONFERRED ~niv. of Michigans Ann Arbor, Mr Indiana Univ.s Bloomington, IN Cargegie [nst.~ Cold Spg. Herb., NY Univ. of Calif., San Die~o, CA B.S. Ph.O. Postdoc Postdoc 19&9 1952 1957-60 19&D-&3 Zoology Zoology (Genetic ) Bacterial Genetics Biochemistry RESEARCH AND/OR PROFESSIONAL EXPERIENCE: Concluding with present position, list in chronological order previous employment, experi- ence. and honors. Include 13resent membership on any Federal Government Public Advisory. Committee. List, in chronological order, the titles and complete references to all publications during the past three years and to representative earlier publications pertinent to thisapplication. DO NOT EXCEED TWO PAGES. 1963-62 Assistant Professor of Genetics, Dept. of Bacteriology and Botany, Syracuse University, Syracuse, MY. 1967-71 Associate Professor a÷ Genetics, Dept. o÷ Bacteriology and Botany~ Syracuse University, Syracuse~ MY. 19T~-76 Pro÷essor of Genetics, Oept. o÷ Biology, Syracuse University, Syracuse, MY. 1976-B2 Head, Laboratory o÷ Genetics and Carcinosensis~ AMC Cancer Research Center and Hospital, Lakewsod, Colorado. 1982- Adjunct Pro+essor, Dept. o÷ Biochem/Bioehys/Genetics, University o÷ Colorado Health Sciences Center, Denver, Colorado Awards, Fellowships., 196D-63 Postdoctoral Research Fellou, American Cancer Society - Laboratory of Dr. David M. Bonner~ University of Ca'lifornia~ LaJoila, Call÷ornia. 2/63 to Fulbri~ht-Hays Auard to conduct research at the Oepartment o÷ 8/63 Biology~ Univ.of Buenos Aires, Argentina. 2/70 to Special Fellouship o÷ the National Institute of Health. To con- 8/70 duct research in the laboratory o÷ Or. Aizo ~atsushlro~ Osaka University~ Osaka, Japan. ~77-7~ Member~ National Science Fo~Jndation Panel on Genetic Biology. ~/8~ ±o Recipient o{ Fulbri~ht-Hays Auard and Visitin9 Professor, Univ. Los Andes, Bogota, Colombia. To teach course in Molecular Genetics. Selected Publications 1. LaScolea, L.J., and Balbinder, E. Restoration of phosphoribosyltrans- ÷erase activity by eaFtially dale÷ins the trpB 9one o~ the tryp- tophan operon o÷ ~.~yphimurium. J. Bact. 11___~2= B77-885 2. Macrlna~ F.L., 8albinder, E.~ and Bassel, Alix, Molecular charact- erization o÷ a stable F~lac plasmid. Biochem. & Biophys. Res. Comm. 5_~= 737-7&3 (i973). 3. DiCamelli, Ralph an~ Balbinder, E. The association o÷ tryptophan synthetase subunits from E. col i and S. typhimur.iu~ in homolo- sous and heterologous combinations. Genetics Res.~ Comb. 323-333 (197&). 40000201

PRZ;C~.~AL ~,~;~ATOR~RC.~R~'.~ C:~Z~TOm El ias Bal binder &. £altahan, R. ]iI, Oaoley,M.M., and Balbinder, E. A mutation to methyltryptop~an dependence in the tryptophan (trR) Operon Salmonella typ6imurium. II. Studies of 5-methyltryptophan-~epen- dent mutants and their reuertants. Molec. Gen. Genet. 129-143 (1978). 5. LaScolea, L.3., Domley, M.M., Torse~, H., and Balbinder, E. ~ muta- tion to 5-methyltryptophan dependence in the trp operon o{ Salmonella typhim.uriqm. III. Correlation betmeen phenotype and the proper- ties o+ the second enzyme for tryptophan biosynthesis in a 5- me±hy~tryptophan dependent mutant and. several 5-methyltrypto- phan-independent revertants. Molec. Gen. Genet. t65: I&5-153 (197s> &. Anselosanto, F.A., Torset,R., and Balbinder,E. A mutation to a 5- methyltryptophan dependence in the ~rp operon of Salmonella t~phi- m.urium.' IV. Isolation and character}zation o÷ trp promoter muta- tions. Molec. Gen. Genet. 1~: i55-1&a (1978). 7. Dooley, M. and Baibinder, E. Di+#e~ences between ire An%hran}lale- 5-phosphor~bosyl pwro~hospate phosphoribosyltrans#erases Salmonella typh~murium strains LT2 and LT7. J.Gen. 112: 171-179 (1979). 8. 8alb~nde~, E., Re~ch, C.I., Shusarts,D., Keosh, J., F~biger, R., Jones, T., and Banks, A. Relative mutasen~city of some urinary metaboi}tes o+ the antltumo~ dru9 cyclophos~hamide. Cance~ search &~: 2~67-2972 (1981). 9. 8albinden, E., Kerry, D. and Reich, C.I. Deletion. ~nduction in bac- teria. I. The role of mutasens and cellular error-prone repair. ~ut. Res. Ii~: 147-168 (1983). ID Ba~b{nder~ E,~ St~ck~ S.M and Sharma~ 0 K. Comp{ex ef+ects of re- tinol on t~e metabolic activation of 2-aminofluorene. Enu. Hut. ~: 665-678 (1983) II. Balb]nder, E. and Kerry D. ~ ne~ strain e{ Salmonella reverted by mJtomyc~n C and N-methyl-N-nitro-N-nJtrosowuanJd~ne; possible un~ve.~sa~ tester for mutasen~m compounds. Mut. Res. 315-320 12. 8a~b~nder,E. Use of pre-des~sned p~asm~ds to study de;et~ons: strates~es for ~ea~ns ~th a comp~ex prob~em. Cha~ter 40.1n ONA Rep~cat~sn and Mu~aQenes~s..Eds. R.E.Mmses and W.C.Summers (~n press,1988). ;3. 8a~b~nder,E.,Ma~Vean,C. and W~;;~ams,R.E. Deletions on ~es~sned derivatives of plasm~ pBR325 ~n E.cm~. Mut.Res. (~n press,~g88). ~4. Ba~b~nder,E. Deletion enhancement in strains cnnst~tut}ve for the SOS response. (~n preparation). 40000202

Estimated duration of differentphases of project Ist. Year (a) Studies on effect of asymmetric duplications of terminal repeats (objective i). (b)Attempt to recover deletion intermediates (objective 4). 2nd. Year (a) Studies untargeted?. (b) Continue studies above as needed. 3d. Year (a) Participation of RecA-mediated production of deletions. (b) Continue studies above as needed. of mutagen-induced deletions : are they recombination Budqet-Est~mated direct costs for first year. Personnel Dr.E.Balbinder (P.I.,70%) ....... $43,000 Technician ..................... $20,000 Total salaries ................... $63,000 Supplies and miscellaneous Consumables(culture media, disposables, enzymes,chemicals)..$ 8,000 Miscellaneous(publication costs, telephone, mail,copies) .......... $ 2,000 Total supplies &misc ........... $i0,000 Total Direct Costs ............... $73,000 40000203