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RESEARCH AND;DEVELOPrfiENT, NEUCHATEL - QUARTERLY REPORT DIVISION . RESEARCH SUBJECT TITLE . PESTICIDES PERIOD COVERED : JULY - SEPTEMBER 198'8 WRITTEN BY . Amati-D. (DAA) KEYWORDS . culture, moon, saturn, methoprene, tla, statistics, maleic-hydrazide, mhi-30, diithiocarbamalte OBJECTIVES To provide an analytical service for the analysis of pesticides in tobacco and cigarette filler. To develop analytical methods for new pesticid'es and~to improve existing methods. RESULTS Proiect_CULTURE Pesticide routine analysis The new pesticides EDB, DBCP, nitrofen, permethrin and formothion were includediin the routine analysis mid!August [1]. Trials to increase sample throughput in pesticide routine analytical work were performed. Methoprene The method for methoprene in tobacco [2] was revised to obtain a detection limit of 0.5 ppm instead of 10 ppm. The description of the method is in preparation. Tobacco and tobacco dust samples from trials with Kabat and Dianex respectively, were analysed for methoprene. Residues between 1.4 and 78.2 ppm were found [3][4][5]. Pesticide residue analysis A computer program was established to collect all analytical results of the pesticide residue analyses and to be able to performistatistics,

RESEARCHIAND DEVELOPMENT', NEUCHATEL - QUARTERLY REPORT Offer samples At the request of Leaf Department EEC, 12'offer samples were analysed for pesticide residlzes. The concentrations of all pesticides analysed were below the levels set by the German regulation. Three additional tobacco samples from Guatemala were analysed specifically for endrin. No residues were found. Maleic hydirazide Five TLA samples ex PMH and 2'cut filler samples ex PMG fromi special trials were analysed for MH-30. All PMH-TLA samples contained MH-30 residues above the recommended 80 ppm level (Table 1), the cut filler samples ex PMG 45.7'ppm and'63.9 ppm, respectively [6,7]. Proiect_SATURN As an anlytical service for PM-Australia, 23 Bright samples crop 1988 were analysed for organochlorine, organophosphorous, diithiocarbamate (DTC), ridomil and maleic hydrazide (M?-i-30) residues [8]. Maleic hydrazide and dithiocarbamate residues were found'in various amounts in nearly all samples. Residue amounts exceeding the Germanitolerances are listed in Table 2. Project_MOGN PMG-TLS samples and PMG-brands As an anlytical service for PMG, 63 TLA samples and 9 PMG brands were analysed for pesticide residues [9,10,11]. In 22 TLA samples the MH-10 values exceeded the maximum recommended level of 80 ppm (Table 3). In the PMG-brands no pesticide residues above the German tolerances were found!. MH-30 residues in specific brands are showing, however, an increasing tendency. MH-3'0 survev of DB-brand's, June 88 Compared to the last survey of Dec. 87, an~increase in the MH'-30 residues in 7 of the 12 tested brands, including the NILK-DB, was observed (Fig. 1) [12]. This trend is confirmed by the results ol~; the monthly and auarterly check for MH-3'0 of the MLK-DB. O ~ ~ ~ W Q1 ~ ~ N - 15 -

RESEARCH AND DEVELOPMENT, NEUCHATEL - QUARTERLY REPORT REFERENCES ['1] Pestlin-S., Amati-D., Quarterly Report, April-June 1988, p. 14. [2'] Amati-D., Quarterly Report, July-September 11957, p. 12. [3] Memo from Amati-D. to Freymond-J.M., July 19, 198&. [4] Memo from Amati-D. to Freymond-J.M., July 26, 1988'. [5] Memo from Amati-D. to Freymond-JI.M., Auqust 18, 19!88. [6] Memo from Speck-M. to Babey-J., September 2, 1988. [7] Memo from Speck-M. to Babey-J., October 3, 1988. [8] Memo from Speck-M. to Wa:jntraub-J., August 12', 1988. [9] Memo from Speck-M. to Probst-K., July 14, 1988. [1i0] Memo from Speck-M. to Probst-K., August 12, 1~988. [ 11 ] Memo from Amati-D. to Speck-M., August 22, 1988. [ 12 ] Memo fromiSpeck-M. to Fink-W., August 9, 19'88 .

RESEARCH AND DEVELOPMENT, NEUCHATEL - QUARTERLY REPORT Table l: MH-30 residues in PMH-TLA samples LOT' ORIGIN MH-30 ( p. m), 3711 US 90.0 3662 US 99.3' 3653 U6 8'6.8 6032 US 223.6 60:73 US 83.7 Table 2: MH'-30:and DTC residues in PM-Australia samples SAMPLE MH'r30 ( .m) DTC ( pm)'. S. Torrisi, lucs 61.0 E. Bortolussi, leaf 104.7 E. Bortolussi, tips 54.2 T. Infanti, tips 101.9: G.G. Tatti, tips 105.6 R. Latif, tips 91.4

RESEARCH AND DEVELOPMENT, NEUCHATEL - QUARTERLY REPORT Table 3' : MH-3&residues in~ PbiG-TLA samples exceeding the German, tolerance level LOT ORIGIN! . MH-30~ (,ppm)~ 3629 US 92.5 3630 US 89.1 1918 US 298.6 1927 US 320.1i 1826 USS 212.3 1921 US 197.5 1951 US 82.3 1984 US' 197.0 6031 US 183.3 6086 US 81.5 6087 US 88.5 6093' US 325.8 60 97' US 190.7 6295 US 85.7 1822' US 319.0 1827 US 174.3 1909 US 215.1 1926 US 187.9 1965 US 90.2 1978 U S ' 287.2 1979 US 195.8 6093 US 2'59.4' - 18 -

6e-, 3 z I i % % ~ a a a a a a a i 94VsCsezoz La~ ~ MH-30 Residues in DB-Brands, 1987-88 a ~~~~ a Qya~'~ 5'`yJ ~b a,n Ya11 0 DAA101, 31-10-88