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J. Cell, Biochem, (1990) Supplemeni ]4B F 606 HOnMGKD iNDUC=D NYDRG_YSIS = L~_=~. C:`-"-rCSc L_•~_- -`-- oY PHCSPHDLIPASE : AND .. ,,. 270k GF SECOrlD YESSENCSBS, Ny'es A'_ton Jo,nes Cell Science :_rter, _ake P~~acid, NY ~ of _e'.lula- p:nesp-c'..ese D(?LD) -ave raisec Studies on hormone-irdu::ed activa.-c- several interestinc q~est.ions conce- `ne :re ro' es o.` non3'~esunr:incs`tide-derived phcs- phatidate (PA), ap.roorted modifier o= ce'.' -es~onse, ar-- cle-.ycly=erol (DG). In orcer to address these o.,es.ions, exam'.na:'.cc c :re aoss~ie tion of Pnospholipid degradative pathways and tneir teTN7'a1 re'a:'on5n1^ m,.st _E :SCer:a',ned. In KEF52 rat fibroblasts, prelabeled with ['Hlaracr'denic a_id, vascpres_ n(VP) treatment inducec phosphatidylinositol 4,5-bisphosDnate (DIP, ) rydroly<is by =_= (reas.:^ed by ['4]DG forma- tion) at 5-15 sec. A: 15 sec tne ine.ction oi P_', - acc'vity, ,e,:s_rec by the generation of PC-derived PA, ensuec. With ethanol i, the z.,':jre medium, 'dF e'icited the formation of PC-derived phospnat'eylet.hanol, (ca:a,q~ee 5y -LD transnhes,na:icylat!on), which occurred with a time frame t-at parallelec .re `o-rtaticn of PA in tr~ aosence of ethanol. PA generation from P' eas maximal a•_ 2-2.5 ,-.. , a: whicn time t•E ..--mation of PC-derived DG was initiated. Hormcne induced PLD activity was also demen<_trated in vascular smooth muscle cells and endothelial cells. -nis worVk illustrates -.~r complex orchestration of lipid events _:^:at accompany the DiP; sig^al t-ansducticr uath~,ay, and underscores the importance of future investigations tc define the role of ~I i ~rd nonphosphoinositide- derived second messengers.