Tobacco Documents Online

:.t. c, z_ t,i 7 C.' (< flF i C! TF'3 CCS2PC8I2IC3 07 CF4d.4nE7g SNO2C5 T?.^: LF F3:;,}iTS 77!f<,vl> John L. Fithsl aad S. T. R<sYSas Ohio 7:Cata tPni`{.rait7 {AbstracC~ aigaratts sivks hnra bac¢ reported by a aumbar af diftnrent inrast2gators. lrnng the lmom ° cercatitssents nf ths hvzaous phaae of the grinaipel coks stromi are casban monoxidc. earbon diozidaz hTdregen • anliido aa4 hpdragon cymida. Zn tha nr.eaat rork on attnmpt ess sadr to doterains t3aesa ecnetituents aa aer em•atal} ss posrible wdar conditions sinviatiag thoae of emoktng. T1» anauat ot cerbon nonoziES 2a a2ioca ta cerrss7wnd to Tsttcsen 2.1 aad 3.1 aI CO gar cigarstts. 8fdregen sssifids is present ms D.flD13S by Toisses ot the _ gsssaxs phaas and bydrogen epaaids vv not detocted. ` 1asE72caa was Io=d in ths aigaretts anaka end the pras.aes of otlnr uruatzsaLed hl'drooarbons irsdiaatsL. s A csagarwtiso rassaroh cantributian of the P. irsi2lard Co. a.ad the flg4 Re+ earch Facasdwtions 0

The train canaietsd of the ZolloxinQ partes (1) A bubblar santaining a saturated eoiutioa of in 1t2 sultvrie acid. The silTar auifsts rssaras hpdrochlorio snid aad h7droQ a. . •sulfide troa the {;as. , (2). A Lrfdrsis bulb filled sith `IIrierite' to renan sioisturs, (3) A L+Sdsia 0 the earbo ,(!) A L'idrale ];uib fillad with "IIrierit.e' to prarent satranes o~ naiatcQS irca thu rear. The antire dra:lzgg thrnugh it ths s=oke ot flre aidarettes. At the sed otthis tixie the seir;ht of the ;taeerite bulb sas rs- ~ . Ths train vsa iffiedistel7 roscasabled and fixe aars cifarettas snokad. This procesa ssa rspes4ad iutil good ' check results sen obtained, sa•foilosat _ + I}ateraina. ' Ssi€ht Yab+as CIIg . ' tion ~ C6~_ ._in rt, . Assrags ?T53+ss q.EEOC 140.9E

u The tirst natsriai ariiah cssss through had a ctrhon-h7dregan of atcst of tar7 s7sariy 111. to aa5ap cut the ramainiag nttariaZ. This L't.Cdn . 4.Db92 g. St.II9U 0.0{77 g. iJt.1 ~L,9 lStar tha hoiiixg iaad starad dam sw.rhtt ot-'arr fractiona tare obtained rtctch gare t ecaait.arabiF hifihar ratio of hfdrogen to car8aa u the tecatM Zatatian. Et.3'"4 8m• S cigcraitas thia souid ba . , - 0.O40.S g. 04179 g. 4hasa rasuits appaarad-to issdiettt the praaence ar ~ uniatssrstad h7drocarboas in the anota, xisiZa the ratio firat ~ obtained tugyestad the 7rasoacs af tcet72esa. The relatiral7 ~ ~ • ~ hLgh rctios of &Fdrogaa to car7rm in tirt Last ptrt of the disti2ltta tagid be azp2ainad at being dua to the grssanot ~ a) of ditfarant h}drecahazui ar to trteaa cf~aciatura carried 43,:a94 S. it. ££s.y 1S I Ratia cii£- 114 PinalZfr the trap saa aarmad b7 a ratar bath and nitroGaa 0 ~~..~~

Za rcast or tho Yarks a p7rax tuba 3 ca. ia diaatar and 3€S cu. ioag, packed ta a dapth of 23 ca. with 2fg ioeb, singie turn, i`Jrez glana haiiaes, su piaaed ia the train iffiediatei7 ia back of each eigeratt. boider. 5uch a caiu3a aitactixclr trapa the 1iquld eoastituenta or the emora aad ia sasity cicaned.. The iife af such a cniuen approzinnta 594 cigarettae and the tsr a.T be aztracted troa the catm', b7 suitabie soisont.. Precipitation or tho cciloidai aaake garticlas b7 n silant aisatrical diacharge aas accosplianed by passin; the snoka thronch a. 12.5 iach Liebia Coadanaer jacket carersd ces the outaids with tisLoil and haris~- a ceatrq.71 eleatroda of if9 inch stsiniess stan2 rod b.id in piaee tT rubber stappars. The tinfoil and ceatral electrode nare attached neparntioa ia aFfsctira but ~ hsa a dissdrantag. in that aono aaone is producad rhich fii}5at ozidiae aoas or tha reducing coaposads in the aoake.' restigatioa intolr.d usa or tha Ez=ia1tirn or the IIaaoaus thaaa _ ' a- Deteminatim.x of Caricn DLazSCe Carbon diazida ru dstsrxiaad bjr psssing'tha Cas.ms phasn af the saoka throu?h an adsarptioa tr.in -• keaaribed in Pouikla nates on qn.atit.•a.- anaipais.(2i • 000 ro2t travforaer aperating at 12

i i: ','j '- €'~.. ..; t:. _' i.au,cxnr,ixa 1.# 1 C: O F i i F# IIaing t atsndasd tonttir.ing O.Nt?i35 g. Rg9. 80 ai8+u'*ttas gs» a raic5a of 0.:3D3T5E C. SigS {a+rarcQe of tsa runa). . . • esas atcadard, 40 cic,nrattee rara t TelcS of 4.0`Y.}L75 p,, %.4. 4'hsas Taiuas aarr+apond to ueiELt at 0.012 siliigrs:s p.r aigsrstts or s.oiuca Dnreent of - Q.t7IIi?}'l12g In the [aokl. _1 IIntnre3natien of 7[edraran Cva[sida S1ia prenance vf tracas of h;dragaa tFenida In tignrctts , p¢oke has bacn repartad VT fchalloru')i ;tasor k fi'.ahiiCi¢) - snd da Caaposs'`ff. The cuthore Oare uan~a3eA horesar, to detect this compauad bI ap„Iicatian tt tLs STsualnn 92ua Sast 3r:;Ich is t.`>s sost aanzitiva egzcific aathot for hjdrogm t7snide. It is pnsaihta thonPh t2Fat anp ESCdt preaent aaT hsre been ramarad LT the tar trag siacs 3eha22arfi31 reported as of catto¢ filters affactFralT fiitarad It aut. Cj {77

'r7*.~ C03F'fv2FSQS aA fI'3k7!~i78 ~3F,^..~8 7IF': C~5^079 7Tfi.:.e a The corpositiaa or tobss:o aaaYs is a matter ef ccnsidsr- abia interaat. partieularl7 ia xiax or its paasibls phYtic2ae- icai efSaots. Iareatigatioas af the acnstitusuts or the ~" snoke ha>a been reported principaitT In nedioai and pharas.. cauticai jaurnnls. 7SuaIa or it has beoa qualitntire Is aatura aod only A smal2 part has been ctrricd out sm3ar aoa- dit2ons timilnr (o aorva2 ana$ing. The prasant <ork is aa attsapt to detorrrrrr~~~}iae as acourats27 ss passibLa the nature sad a=o+mts or t#ja substances present in the saaka. uadar canditiona as ii4FFFf those or nornal aa~Ying as petaibie. ~is paper is coa'aeraed grisariij sith the coastituanta at the gasaaus pi:as,~ at the principal snake strnnaF i.e. that i part of the anaYe drasa ttxaugh the cisaratt.. The Rnakinr L:acAina . Zu crdar ta calleot avsosuts or cigaratte saaks auf- ricioat far, aasl7s anchins ia n.cassarT. 22cis dsfia* s3aa¢Ld sinulato. as claaaip aa posaibi., the aatioa at the as.rags sacker. iba a.ci.ine osed in this work it3 eza.pt far air.or chang.a the asas u that aapla7ed by $radford. Iiarlcn and Eazzmsr.ft3 Siace this acehfas Ss dascribed In their pubiication repetition .ou2d be superfiss- ac.. .'.nftice it to stT ttst by s suitabla arrangs.ent af a wtter 6ars:te anII a four rar ta2cs actoa4sd bf a aator.

ftaaz is applied altsraat.l7 ta tato- cif,arattas in a ammaar vor7 lirs the action ot a smaksr. 8ach cig.rstt. 2s p,ffad tor two soconds azu0 each cinuts ia ¢uch a r€1 aa to dras 40 ah. of air t<`zraup~x the eigaratta. The rs- sclting principal scaka atraax ia led thraugh a dariae which segaratas tDa nan gaseaus part (Si4uid ar ealid) af ths aaak* frw the gaseoua. The iattsr ia then conductad tSrms3h a train ot atrsorb.nts such as Is naadod f= the dstsrainatian t»Sng xsds. Under noraal conditiona it Is possibla to aaoka about 2W oigicrattes iis azt 6 honr daf. 4 ftrs cigarattcs used are a, standard brand provided b7 the aaspsat a•hicT4 sponsors the inraati~atica, F€c att=pta Lare baan nad..to condnct corparatiYS tests uz Qitfer.nt brands af Cib;ratta3• dcnnrni Gherr_cteri It b.s bean recorded litarature >_~d ccntirsrod in our laborator7 that th. principa2 sa:ak. strsxx {t•'ut dreva thrfleerQii tha siagr tcid.ia rhi2e the secaaCar7 ancks streas (t`orssd at tbt dig*iag and ot the cir.;ratts, betrran yatZs) is s3kaL'n.. This snggasts that tE.a ascandarT atrsna aaf contain aobstances not fmad in thrv priasrs atraaa. 'iha pr.aaat in<e.tiCsticn, keak. straaa. hossx.r, has beon confined tc axt erasiaati= or ths prineip.I

~ i L t.i ccs.z { .. aka 4•aa cigara appears to cantain a aach grester sa+uit af carbon aEUnazid.. Zhamss(3'i') hoanvers a ot' cigar smasca using a hc4cg2abin taat ahorinp, 7.42 mi. Co par grnn. ". . 4'!xe r.+asona ior such tariations, undoubtsdlT lie in the raanar cf nnsiysiss in the netbcds of rao:-1ng and ig the ears with which IaCarlsriag sub;b+maos are roao-r.d., Proc.durna based cw the redncing power of carboa nonuzidsi such as us~ of iadine peataside, or paitadi~n chioride are au6Jeet to aeupicioa rsniaaa other roduaing anbstaacas are u accr,ietaiy rasored or ahosi to t» absent. i7et.ra:nationa based m use of cuprosss' chloride ara alto suhjact to cur- r.cticn if acatyianic bydrocarbena are present. Saroral diFturant nethods Sarn tried in tEa proaeat rork, and tfm nethod iinaiSf decided cn waz to resare .rerT carban cosPo*md poaaibie azcept c:rbon aaonorf.da and tiaa to ozidize the latter vith heated copper oxide and detornina the r.avltin> carScn diozide. 2he absorption train far this detcrain.tioa was aa e, which ia aqvisaieat to about 33 aEi. per aiea.ratte.. A bubbler tubs containing a.aturat.d solution of aii'sr, .uifats in itl sulfuric acid (to rocon &C1 aad.E$.7}. (E) Driarit# in a kidrais bu2b ta ras.ara aoiaturs. {3} Ascarita in a YSdra1, bulb to raaors carbaa dioxide.

Saparntinrt nt the Cul .,~ ~r.,nrn~~ ~>s Tobacco smoke consists of two distiact phases, a gaseaus phase ahich includes such aoapounds as carbou dioxide, hidragan suifidst carbcci nonazide and other aatarials shich ars gaseoau at ordiaarF taaporatsses, and a'13qvid' phaae shich consists of particiea. of coiloida2 size, which sre or saiids at ordinary teapernturas. In preriaus EnrastS~aEoas a nxab.r of diffaroat methaGs i1ATe bfe6 Steed ta separate the p2[Saes. EraR(tardT iI2r12n andSassaer(lI collected the sacke in a 1arLa flaak and si= la+red it to settle. Qthar iaTesticatars gassed the saoks thraupp solvents auah as e2zho.ona or tlxroc;j~ su2furia t snid. 'fhe sed4aentation afthod appeared to b: too slow and _ ` the uae of solxents introduced naa eonpounda into the snaoous phus. ButiVria scid could reao:e nane at the gaseoun constituents. It raa decided to trf to separata the ZI$nid partiaies by takiag advsntegs of their colloidai properties such as rdaarptioa on t Large surfaas or precipitation by a silent eiectrica2 discharge. In sous preliaisirl experiu.nts the rmks sas dramn thrauf.h tubes paoksd loazelT with filaaa rool. This darias tu canpletelT affcctira but it ras found that the rerj ~ large surfac. ot the glass wool resulted iu such prassnead ,~ adsarption that it sss difficult to sztract tfie tarrl ~ taat.riai. In later sxperiusata saoti gius h?.licea sra C1~ substituted lar ths gi.aas raaL with axcelieat rarutts.

Zf it ia nsa¢aad that c1I1 tha vsrboa diazida is fc.ud bf oiidat£on oC earSoa aoaorSde, nsstecting tt:a h7drogeni thia vou2d regresaat aprirozLxatsl7 0 ai. CO per cigarettr er aboat 3.0: C?i ia the snota. It vouid aaron obvioua, -ho.ax er that eithar hydrogeu a£a¢e, or uwter vapor or aova h7drogen tnntsiniisg organia can;ovznd ras greaent In the •soke, and thasa resuLta tra thorarara not re.F.tabie. Innortioit ar additioaal driarita ba;bs In the trrSa did net altar the a=oimts of nater and cnrbon diozide forAad In tba eosbustioa. air was placod just bafore tha combus train, xs the £Srat nbaorbint. h=drGd ciy,arattes sara saakad and th> aake vaa led thra tha eoapi.te train. hnaiTais a.rs neds ef the gas pasaing thrmzv3t the trap, m the E:si: or ateZ1 S cigarrttos. Altar the 170 aiFarottss had bosa caokad it res obaarrad that a laa12 szosmt of t uhits solid had ao)3act.d In tlsc t.rcp. 4cctifirsnca of L'za+tKcr.ntcd tF9f.racarbana ia C?-rrntta :->~}_a In crdar to ob6ain an sapros!ste an¢I7a£a'of the trcppod aat in the ezparinant doraribad nbo.., the aeehisu was stoppad, aad the liqnid air bath ramosed. +Ts .hit- solid In the trap ias +t17.os.d to asit and tha rasulta.a liquid permitted to boil aYayt a Littlo at a tS~s, throsZja the oaa7vrtioa tube# thua afSerdinC a ro+vz.t i7aetian.tim.