Tobacco Documents Online

Cigarette smoke was passed through the separating appa- ratus and then along the absorption train. In Steps 1 through 3, everything but carbon monoxide should have been removed. At Step 4, the carbon monoxide would be oxidized to carbon dioxide. If any gaseous hydrocarbons were present, they would also be never fully satisfied that the amount of carbon monoxide accurately determined. ide and water. Significant quantities of water and carbon dioxide were found in the respective bulbs in Steps 5 and 6. This indicated that some gaseous compound con- taining hydrogen was coming over withh the carbon monoxide. Not all of the carbon dioxide found in Bulb 6 could be attributed to carbon monoxide. The presence of gaseous hydrocarbons was sus- pected. Another trap, immersed in liquid air, was inserted in the absorption train between Steps 3 and 4 above.l4 This cold trap removed an unidentified white solid and resulted in lower weights of carbon dioxide and water. The continued presence of water indicated there were still impurities. Dr. Haskins was oxidized to carbon Subsequently, the white solid captured in the cold trap was analyzed. Upon gradual warming, the white solid vaporized and was passed through the remaining absorption train. The re- sults of this analysis indicated that acetylene was present. it was also noted that fractions of this material had the charac- teristic odor of unsaturated hydrocarbons, such as cyclohexidene 89`J495,24 - 5 -

Components a8t7 Wynder & Hoffman Carbon Dioxide 7.77% 8-10 volume % Carbon Monoxide 0.48-0.96% 2.7-6.0 volume % Acetylene 5 mg_ i 10ft Cig. 3.0 mg.11€t4 Cig. Hydrogen Sulfide 1.I8 mg./10tS Cig. 2.7 mg.f100 Cig. Hydrogen Cyanide (Cig. = cigarettes) None detected 3.2-11.5 mg./100 Cig. E. Summary. The research conducted at Ohio State University from 1946 to 1948 was primarily a chemical analysis of cigarette smoke. Chemical analysis of complex mixtures during this time period was a difficult, time-consuming task. Dr_ Parmele of Lorillard seemed satisfied with the work of Mr. Fishel. He was dissatisfied with the supervision supplied by Dr. Haskins. Mr. Fishel decided to leave Ohio State University on April 1, 194$.21 Dr. Parmele did not recommend the continuation of the project with another graduate student under the supervision of Dr. Haskins. Lorillard's association with Ohio State University thus ended. PP/cb -1fl-

REFERENCES 04359619/9638, Progress Report 7 from SBF & JFiA to Lorillard, 03/03/47, pp. 15-16. 04359543, Progress Report from ,7BF & JFH to HBP, f77J16J46. 3. 04359346, Letter to HBP frorc JFFI, 08/07/46, re Reader's scope article, Roffo & Grace. 4. Grace, E.J. (1944). The toxicity of combustion gases withh special reference to tobacco smoke as a carcinogenic factor. Medical Times (New York) 72. 332-334. (Harris Report). 5. Grace, E.J. (1943). Tobacco smoking and cancer of the lung. American Journal of Surgery 6O: 361-364. (From the refer- ences on p. 68 of the Jeffrey Harris report) 5. Roffo, A.H. (some 17 references are given for Roffo from 1930 to 1943 in the Jeffrey Harris report. Most are in spanish or german. The 1942 reference is for the article Experimental carc_nomaa of the lung by tobacco "tar." (Bulle- tin of the Institute of Experimental Medicine for the study and treatment of Cancer 20: 103-122 (spanish).) 7. Roffo, A.H. (1939). Carcinogenic 1:2-benzophyrene extracted from the "tar" of tobacco. Boletin del Instituto de Medi- cina &xperimental para el estudio y tratamiento del cancer 16: 1-38. - - Bradford, J.A., Harlan, W.R., fianmer, H.R. (1936). Nature of cigarette smoke. Technic of experimental smoking. Indus- trial and Enqineering Chemistry. 28: 836-839. (Cited in Jeffrey Harris report. Affiliation with American Tobacco Co. inferred from the documents [i}4359544 and 84366767j.) 9. 04360766/0781, John B. Fishel and J.F. Haskins, Ohio State University, Manuscript, The Composition of Cigarette Smoke The Gaseous Phase, no date ( 19s7 interred), pp. 2-3. 10. Ibid., pp. 4-5. 11. Ibid., pp. 8-9. 12. Ibid., pp. =d. (See also 04360741/0745, Report 8, 435/t71J'47. 89749530