Tobacco Documents Online

Topical Report NCI/S&HP/ORNL #52 FINAL SMOKE AND CONDENSATE DATA FOR THE FOURTH SERIES OF EXPERIMENTAL VARIANTS October 19, 1977 W. H. Griest, R. B. Quincy, Fi. Kubota, A. H. Marshall, P. Mraz, R. Reagan, G. Olerich Tobacco Smoke Research Program Bio/Organic Analysis Section Oak Ridge National Laboratory Oak Ridge, Tennessee 37830 Interagency Agreement (ERDA-NIH/NCI) No. 40-485-74, Part I Internal (ORNL) Contract Charge Number 3390-0225 Intended for i nformal corr,muni cati on wi th project management only. Confidential until published or released by author.

FINAL SMOKE AND CONDENSATE DATA FOR THE FOURTH SERIES OF EXPERIPiEhiTAL VARIANTS W. H. Griest, R. B. Quincy, H. Kubota, A. H. Marshall, P. Mraz, R. Reagan, G. Olerich Introduction: This data tabulation represents the final report for smoke and condensate constituent analyses performed on the Fourth series of NCI experimental cigarettes. A summary format is used to facilitate compari- son of the variants. Experimental: All handling and analytical procedures were NCI routine protocol methods described in previous Topical and Annual Reports. The analytical protocol consisted of two analyses, each in quadruplicate, of freshly generated smokes. Three skin painting batches of smoke condensates were analyzed in duplicate, and one composite batch was analyzed in quad- ruplicate. Special studies were in quadruplicate on only one batch of condensate. Results/Discussion: The data are divided into three sections: Average Values Standard Deviations 95 Percent Confidence Intervals Each section is further subdivided by the following units: Smoke Data: Delivery per Cigarette Delivery per Grarn of Tobacco Burned Delivery per Puff Del i ve.ry per Li ter of Smoke Delivery per Cigarette Relative to TPM Condensate Data: Delivery per Grarn of Dry Condensate The following routine protocol constituents are TPM H20 Nicotine Tar Smoke Data: Acetaldehyde Acrolein Isoprene HCN • reported: TPM Phenol i cs

Condensate Data: Ni coti-ne Indole Skatole Benz(a)anthracene Benzo(a)pyrene pH Weak Acids In addition, one batch of smoke following constituents: Very Weak Aci ds Total Weak Acids Total Free Fatty Oleic + Linoleic Palmitic Acid Stearic Acid Neophytadiene Catechol Acids (C16-C18) + Linolenic Acids condensate was analyzed for the o-Cresol Phenol + Cresols m- + p-Cresols Colorimetric Phenolics Phenol Glycerol It is to be emphasized that in Nicotine Alkaloids these tabulations "0.0" means "no determination made" while "0.00" means "an analytical result of 0, or not significantly different from 0." Comments: This tabulation supercedes all preliminary reports of Series IV data. .

Cigarette Characteristics Code Cigarette Cigarette 4ieiqLt, ma RTD, rnn7 E120 Puff Number 02 1038 44 7.7 03 1208 169 6.9 04 1215 98 10.4 05 1080 63 8.9 06 865 81 8.1 08 1055 92 8.0 09 1048 71 7.9 10 1375 63 11.3 11 1090 89 9.5 12 940 60 7.2 13 1061 86 9.5 14 1183 88 9.9 15 1010 121 7.6 16 1595 >500 7.8 17 1140 71 8.2 18 1073 85 7.7 19 1019 54 8.0 20 1246 66 12.8 21 1055 64 7.3 22 1165 62 8.8 23 1115 75 9.5 24 1110 70 9.7 25 1067 100 7.7 26 1277 57 10.6 27 1205 63 7.9 28 1110 51 8.5 29 1193 93 10.4 30 1122 73 11.5 31 1076 65 9.7 32 1203 83 10.1 33 1110 55 8.2 35 1466 340 7.1 36 1002 61 7.4 37 1130 123 8.0 L8 1166 89 13.5 M6 1055 66 11.3 68 1132 54 9.6 NN 1141 64 9.9 LN 1116 67 9.5 A 1210 ' 92 10.4 C 1235. 43 8.8 D 1250 53 8.0 38 1205 95 11.1 Gb 67 1190 59 10 0 W . ~ G;! ~ Ctt GD W

SUMPIAI2Y OF AVi•RAGE VALiIES - UFLTVERY/CZG.ARF°I`Tk: COB3 TF: 820 dUC T1',H PHENOL IiCFTAI. ACROL ISOPR't F1CN i0R?7A7. .0X CQ C02 G p n i7 i? G tiG i~CG P'3CG 'riCG ~iCG i;CG z~CG I•.CG sL M ;. 02 ~ BV.`O 4 2.f2 0.23 17. 18 56.27 556.13 86.25 1614. 38 E9..r2 50.24 82.00 11.86 21.6? 03 c.31 1.15 0.23 6.89 0.00 303.75 59.63 128.13 139.56 18.26 268.70 12.51 25.91 04 31.9n 3,6 C 1.85 26.39 131.85 °91.50 109.25 537.$8 348.21 L'3.41 5 479.49 17.50 ;3.91 05 3.'..23 4.7C 1.85 26.60 73.45 1156.88 174.13 401.;38 291.56 59.15 78. 17 18.13 241 .77 06 27.54 4.9E 0.21 22.33 62.90 880.75 188.63 '273.?5 239.11 115.38 54.36 21.28 23.89 08 19.47 2.47 0.64 16.37 90.65 800.50 118.38 253.13 204.44 28.47 470.12 14.1? 29.06 09 71.19 3.31 0.98 16.89 51.43 6F+~.7: ii 700.1.3 '274.38 225.27 44.35 226.42 15.03 21.43 10 33.97 3.41 1.73 28.83 120,010 996.75 108.75 392.50 373.94 40.35 573. 84 25. 87 40.72 11 2C.9G 4.13 C.71 24. 12 127.80 989.88 109.75 510.63 371.89 35.05 371.51 14.09 28.1, 12 20.25 3.33 0.66 16.26 60.02 861.75 152.63 268.13 216.79 53.87 157.12 15.77 21.88 13 29.44 3.86 0.16 25.42 144.7{3 775.75 93.75 ;1G.88 281.34 30.52 1111.57 13.65 28.67 14 31.13 3.44 1.91 25.78 142.93 949.C3 109.00 535.63 34 5.89 35.78 432.'74 15.77 32.28 15 11.42 1.83 0.1E 9.41 56.55 546.50 95.38 0.00 146.12 35.42 1112.26 8.89 22.14 16 0.79 0.1?, 0.flC 0.66 0.00 416.00 94.75 50.50 17.85 8.77 99.45 3.46 20.34 17 24.46 3.24 0.96 20.27 48.46 665.88 97.50 374.33 34.3.6 1"34.0G 430.37 19.65 23.80 18 18.60 2.08 0.16 16.36 67.52 741.88 92.13 194.38 360.17 28.63 228.67 19.74 25.37 19 22.77 3.69 0.90 18.1£1 50.30 744.75 105.63 197.v0 313.09 34.55 278.55 16.60 23.36 20 42.40 5.83 2.18 34.39 252.35 1211.25 173.75 540.63 302.35 43.78 59.12 13. 5E 29.81 21 19.82 2.3C 0.44 17.C7 62.17 433.25 51.88 253.75 160.06 26.68 454.97 17.30 22.04 22 26.70 3.21 0.36 23.13 109.37 603.25 93.38 321.25 248.37 57.52 348.62 22.02 31.27 23 34.98 5.26 1.64 28.09 156.55 1015.50 123.38 544.38 - 30.73 479.94 34.27 30.5 9 24 27.15 3.9 E 0.85 22.33 92.52 799.25 111.38 335.63 260.59 31.90 350.•50 15.45 34.07 25 15.53 2.44 0."5 12.64 32.03 679.88 83.63 121.25 340.C0 29.95 316.80 18.09 24.71 26 34.26 4.00 3.25 27.02 205.15 528.50 96.38 428.75 265.46 29.97 1399.37 12,21 34.83 p6S4c46Q

£U 1i9AiiY OF AVL:j.AGL VALtJES - D2LTVERY/CTGARETTE C~") DE Thi'l G ?:2fl fSG NIC (iG fiAR t G FiiENQI, C4CG ACETAL i'fCG ACROT.. TSOPF?tJ MCG MCG , aC2~ MCG TOft1.IlL MCG tdOX MCG CO hs"L C02 N L ?7 17. 87 3.53 1.06 13. 27 51.55 480.25 81 .88 65.38 215. C3 17.58 313.92 12.16 26.63 28 __ 19.99 2.91 1.12 15.96 45.35 773.8f_i ~ 79.38 19F.63 96.22 41.44 •69.03 12.25 25.43 29 30.£t; 71 3. 2.02 25.12 17C~.55 983.88 110.13 536.88 319.01 31.67 ~t41.51 15.25 32.35 30 43.81 5.5e 2.60 35.63 214.07 ^ 1057.63 109.50 575.63 416.17 29.6.3 234.27 16.7 8 34.~32 31 32.13 4.12 0.89 27.12 160.92 678.63 85.25 507.50 352.87 24.63 471.31 16.99 31.79 32 31.44 3.83 1.91 25.69 132.07 1014.00 110.13 521.25 324.90 32.81 449 61 15.77 .33. 18 33 25.12 4.20 1.1"r. 19.75 66.10 847.88 121.00 268.75 2491.39 32.17 216.56 17.34 26.23 35 2.54 0.51 0.09 1.93 34.05 303.75 71.38 76.25 0.00 9.75 174.39 4.37 19.88 36 06.97 4.01 0.84 22.11 70.02 1009.25 163.50 329.38 281.29 -32.9G 108.42 17.68 22.32 37 1£i.25 2.9C 0.53 14.82 21.90 897.25 123.75 226.25 326.51 19.64 901.23 19.67 27.51 38 35.50 4.12 1.73 29.65 152.02 772.88 80.88 488.75 363.81 31.47 526.84 17.46 33.16 %F3 39.11 4.49 2.68 31.95 340.63 8$0.50 111.50 281.25 340.24 40.81 49.05 11.93 31.36 i•1f'i 34.C0 3.27 2.48 28.25 281.23 629.00 92.38 219.38 347.84 36.86 69.32 13.35 34.54 68 27.95 3.41 . 1.56 22.97 98.65 863.50 66.00 375.63 250.24 25.17 276.94 13.65 27.60 i?N 33. 62 4.18 2.64 26.82 164.07 818.63 110.63 524.38 266.97 27.83 522. 64 .'16.09 30.24 Lt; 27.93 3.04 0.74 24.13 140.43 711.50 93.50 448.75 345.25 28.78 513.05 13.08 26.11 A 33.M1 4.30 1.65 27.56 152.8C 767.63 83.25 476.88 345.27 32.27 492.74 18.00 35.39 C 6.90 1.03 0.00 5.87 36.67 205.63 11.00 0.00 0.00 7.20 6.36 4.16 21.80 D 6.32 1.36 0.00 4.94 9.38 182.00 48.38 1.19 0.00 8.34 7.70 4.65 22.68 67 33.59 4.5° 1.84 27.16 165.95 948.50 79.38 461.88 339.42 36.29 537.06 17.51 34.59 41 33.C6 3.2S 1.80 27.97 127.02 1035.75 109.75 522.50 385.97 -37.50 443.95 16.41 32.22 SMUE9

SI3i1i4hltY OF AVERAGE VALUES - Q(IAilTITY PER GRAti OF DRY CO2`iDENSA9'" " CODE "1TCC- '1'S1;F: " G ~•. bI riA- LOIDS i'i G Ii3LOLF (•t CG SKA'.!'CLE MCG E11 A iiCG BAP 2'CCs' i 0- CRESOL i'SG !I hp CRESOL z~iG PHENOL ~'~~ G COLOR P I: aN OL (i x: PE:E'dOL +CRTSCL ~iG 0? 21.45 23.75 193.91 365.45 1.16 0.87 0.68 1.75 5.03 7.46 7.47 03 60.00 53.70 96.93 191.46 1.58 1.28 0.29 1.21 1.61 3.03 3.10 0, 1; 90.20 94.17 491.42 403.92 0.99 0.72 0.66 1.88 3.79 5.65 6.32 05 60.?3 r5 .2 4 z:3.6Z 73.r0 0.99 0.77 0.23 0.69 1.74 4.18 2.66 Ot3 5.14 4.82 6.00 143.50 1.92 1.10 0.26 0.85 1.70 3.26 2.81 08 57,51 63.95 240.46 322.08 0.90 0.76 0.98 2.18 4.10 7.07 7.26 09 78.95 80.21 143.06 277.73 0. 85 0.61 0. 82 1. 82 3.49 5.67 6.12 10 80.53 70.69 426.00 346.31 1.07 0.30 0.41 1.62 3.42 5.27 5.47 11 41.62 37.08 678.92 435.92 0.94 0.82 0.41 1.61 5.79 6.56 7.81 12 53.39 53..05 8.69 200.54 0.79 0.51 0.56 0.96 2.14 4.56 3.G7 13 12.44 13•.87 629.33 '404.31 1.02 0.75 0.40 1.52 4.93 6.42 6.35 14 87.70 79.73 390.54 343.67 1.11 0.79 0.47 1.17 2.98 5.50 4.61 15 29.09 25.88 59.83 22.3.0!) 1.29 1.02 1.05 1.64 2.87 5.75 5.55 16 16.95 0.0. 0.00 9.83 6.14 3.47 0.00 0.00 0,00 0.0 0.00 17 66.35 52.40 187.87 334.69 1.17 0.81 0.57 1.19 - 2. 94 4.62 4.70 1s 15.65 12.42 83.27 371.17 1.00 0.88 0.67 1.23 2.44 5.11 4.38 19 57.77 58.39 10.85 189.0,3 0.69 0.61 0.56 1.43 3.54 5.87 5.53 20 30.13 70.01 16 8 .80 215.00 1.46 1.05 0.38 1.29 2.95 5.70 4.62 21 77.87 68.12 8£,1.50 406.00 0.74 0.67 0.40 1.31 1.72 3.45 3.42 22 18.67 17.88 260.69 438.62 0.8.3 0.69 0.58 1.52 3.39 6.50 5.43 23 74.93 68.24 643.88 402.88 0.91 0.71 0.59 1.72 3.33 6.62 5.68 24 52.82 58.60 128.36 266:30 1.92 1.17 0.40 1.27 2.60 4.03 4.27 25 164.25 44.10 176.54 311.67 1.21 0.89 0.7.3 1.81 2.64 4.57 5.20 .26 175.06 167.19 1459.22 663.83 0.83 0.59 0.59 1.91 4.00 7.06 6.48 96S,4J:f46e

Sliiii';ARX OF AVERAGE VALUES -QIIAINTITX ?ER GRAhi OF D'RX CQNDENSA`1'E NICC- ?`Ii•;E CO'JE ~tG ALKA- LOIDS PiG Z.IDOLE IrC;i 5KA'1'OLN I1CG BAA PICC BAP s`iCG 0~ CRESOL diG M+P CIiESOL IiG FfIEt3QL I:G COLOR. PIi?'IiOL i:G PI'£it©L +CItESOL i~G 27 90.11 87.54 170.31 203.60 1.60 1.11 0.67 1.33 2.62 4.80 4.61 23 75.9t^ 78. 26 360.33 395.03 0.9{ 0.81 0.83 1.57 3.90 6.01 6.28 ,9 80.96 83.83 503.21 356.78 0.96 0.71 0.54 1.60 3.95 7.39 6.09 10 88.20 86.33 433.38 349.56 1.21 0.98 0.47 1.33 4.07 6.07 5.810 31 58.57 58.96 690.22 297.67 0.94 0.77 0.53 1.47 2.89 6.54 4.90 32 97.03 88.80 540.13 356.67 1.55 0.60 0.57 1.68 3.19 5.79 5.43 33 c31 . 64 80.76 141.60 274.62' 0.72 0.58 0. 54 1.4F 3.36 5.93 5.36 35 50.27 44.14 8.15 56.38 3.34 1.78 0.17 0.43 0.46 1.45 1.06 36 60.71 118.39 31.75 157.85 1.17 0.51 0.30 0.89 2.12 4.73 3.31 37 75.40 60.15 595.77 304.00 0.60 0.42 0.26 0.96 1.80 3.21 3.03 38 82.29 77.83 375.15 •293.3'! 1.15 0.87 0.51 1.51 4.25 7.63 6.27 Lu 106.70 103.54 672.15 537.69 1.05 0.83 0.65 1.90 5.33 8.39 7.8£3 :6 122.11 115.68 1014.31 720.54 1.21 0.79 0.73 2.33 6.21 7.85 9.26 68 98.21 87.•18 537.08 352.15 0.91 0.76 0.48 1.31 3.211 5.18 5.04 A 83.73 0.0 508.29 388.71 0.97 0.77 0.86 2.26 ° 4.66 7.65 7.80 C 1.25 0.0 0.00 0.00 4.77 2.32 0.55 0.60 0.42 3.58 1.5~3 0 1.62 0.0 0.00 0.00 3.83 2.13 0.97 1.01 0.62 2.93 2.60 67 74.93 0.0 0.0 0.0 1..31 0.59 0.0 0.0 - 0.0 0.0 0.0 l.~6S4C1.,68

S(1;MARY OF AVERACE VALUES -(Qt1AN:ITY PER GRAM OP DRY CONDENSATE P:i WE iK PiMS VERY TOTAL W-ACIDS `n'-ACIDS i ATTY ACIDS LIii-LIN- PALMITIC OL ACID.`i ACID ST~~ ARIC ACZD *Ii.O.PHY- GT,YCE- TADII:fr'^ 1?UZ CAT'.- Ci': Q L CO*~tf1 E n ~.EQ iiG M`.i i~17 laC l" 1.t1 l ii {j / + WSJ 02 4.57 1.17 0.91 2.08 12.83 6.43 4.01 2.39 5.11 157.10 4.68 03 4' .79 1.30 0.88 2.19 19.33 9.07 5.85 4.42 6.22 121. 3 0 4.2G 04 5.13 1.74 1.02 2.76 22.97 11.73 7.69 3.55 8.92 88.77 5.80 05 4.03 1.85 0.82 2.67 17.99 11.16 3.85 2.97 4.98 91.55 5.89 06 4. 13 1. F;7 1.16 2.63 14.68 6.78 5.114 2.46 6.47 94.36 3.72 08 4.91 1.93 1.19 3.12 16.09 8.09 5.29 2.71 5.39 98.85 4.74 09 4.79 1.90 1.00 2.91 13.35 6.92 3.94 2.50 5.64 131.95 5.16 10 5.011 1.5? 0.91 2.44 18.99 9.78 6.16 3.06 8.45 101.85 5.15 11 4.95 1.32 0.79 2.15 19.00 9.80 6.39 2.80 7.25 87.95 5.95 12 4. Lr3 1.83 1.06 2.89 13.47 ci. 97 3.73 2. 77 4.58 136.67 5.51 13 5.12 1.29 0.81 . 2.09 16.10 8.16 5.77 2.17 7.19 90.25 6.02 14 5. 16 1.65 0.84 2.48 20.35 10.65 6.7-3 2.97 5.97 33.55 5.23 15 =t. €.5 1.77 1.15 2. 92 13.25 6.74 4.311 2.17 0.00 108.80 5.13 1G 5.44 0.93 0.60 1.53 12.09 5.96 3.74 2.38 1.28 240.25 2.10 17 4.73 2.09 1.21 3.30 16.28 8.71 5.03 2.54 5.67 85.20 4.72 1a i¢.30 1.42 1.00 2.45 13.41 7.00 4.11 2.30 3.03 81.24 E.58 19 4.34 1.69 0 .87 2.56 12.97 6.38 4 .79 1.79 6.11 125.20 5.97 20 5.22 1.51 0.89 2.4t? 17.62 9.14 5.81 2.68 5.20 107.45 6.99 21 5.39 1.82 C.69 2.51 15.34 6.19 6.16 2.98 7.62 93.00 3.71 22 4. 66 1. 1+3 0.95 2.3,9 15.91 7.75 5.21 2.95 5.27 77.65 5.42 23 5.59 1.51 0.84 2.34 16.77 8.62 9.73 2.42 6.63 86.80 5.37 24 5. 07 1.53 0.82 2.39 15.51 7.56 5.67 2.28 4.00 66.90 5.91 25 L", . 92 1.63 0.90 ?..53 11.84 6.03 3.67 2.11 3.40 115.22 5.55 26 7.21 1.12 0.73 1.85 9.43 4.12 3.62 1.69 7.48 88.80 2.82 %Si5;14cl+(;8

SEI"1P'AP,Y OF 11VDrAGE VALUES -QUAtdTI.TY PER GRAM OF DRY CQtlDD2fiSATE • ., '~ tiF r, r. VI:^Y TOTAL FATTY LIt.-L7:id- PALNITIC STEARIC NEOPHY- G2,YCE- ChTE - I}ti ACIDS W-rCr!}S fa'°ItCIDS ACIDS OL ACIDS ACID ACID TADIvi1i, ROL CHOL ~CD}? Q iit7 ii ^() a°tG rG i°iG I•IG MG i G G 27 4. 95 1.34 0.65 2.00 12.33 5.32 4.23~ 2.79 2.25 151.40 5. 4 1 28 5.00 1.55 0.89 2.44 12.19 6.12 3.86 2.21 4.09 118.65 5.04 m9 5. 07 1. `t9 0.82 2. 31 17.96 9.27 5.91 2.79 6.75 0.0 4.74 3; 5.22 1.30 0.75 2.05 19.06 8.99 7.23 2.84 8.38 76.35 5.57 :31 4.90 1.36 0.77 2.13 14.18 7.39 4.87 1.92 6.75 93.40 5.41 3? 4 . 90 1.1118 0.82 2.29 15.71 7.95 5.32 2.44 7.19 81.95 5.14 33 4.51 2.08 0.98 3.05 10.77 5.79 3.20 1.77 4.068 115.30 5.34 ;ra 4.79 1.51 0.69 2.20 17.19 7.65 5.67 3.87 3.17 170.00 3.15 3ia 4. 37 11.42 0.84 2. 25 10.97 5.59 3.36 12.01 5. 15 61.00 5.07 37 5.50 1..17 0.59 1.86 7.54 3.40 2.,'-13 1.31 7.34 101.00 2.35 3t 5.14 1:61 0.€32 2.44 18.64 9.89 6.34 2.41 7.03 87.44 5.26 N3 5.05 1.65 0.95 2.60 15.20 7.00 6.47 1.73 8.32 8.44 10.96 ~i6 5.5 6 1.34 0.94 2.29 15.59 6.21 7.08 2.30 9.94 0.00 9.76 68 4.99 1 .50, 0.81 2.31 116. 84 8.50 5.91 2.43 7.30 92.13 5.20 A 5.20 1.24 0.74 1.98 16.99 8.77 5.98 2.24 •.fi.92 0.0 5.28 C 5.75 0.58 0.51 1.09 0.00 0.00 0.00 0.00 0.00 0.0 0.41 D 5.70 0.55 0.57 1.12 0.00 0.00 0.00 0.00 0.00 0.0 0.38 67 5.22 0.93 0.70 1.63 13.30 6.76 4.69 1.86 5.96 0.0 4.38 66s=se