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Microbiological Associates DATE: August 10, 1983 TO: D. Dansie, C. Dove, S. Gosnell, WC Hall, C. McKinney, A. Reiter LOCATION: FROM: RD Curren, RM-David, CJ Henry~ LOCATION: SUBJECT: Schedule for Inhalation Studies Dates For Lavage Serum Study Exposures AHH Lavage Fluid Necropsy Chemistry Chemistry Hematology Rat, Acute, 2130.004 Mouse, Acute 2130.003 8/11 , 8/19 8/11 8/19 Rat 6 Week, Lavage, 2130.016 8/24-10/5 3 wk: - 9/14 9/14 9/14 9/14 6 wk: 10/5 10/6 10/6 10/5 10/5 10/6 10/6 Mouse 6 Week , 2130.005 9/1-10/13 3 wk: - - - 9/22 9/22 6 wk: 10/13 - - 10/13 10/13 Comments: a) Pathologist will be needed 9/14, 9/22, 10/5, 10/13. b) Necropsy assistance will be needed as follows: 9/14 - 12 animals 9/22 - 12 animals 10/5 - 12 animals 10/6 - 12 animals 10/13 - 24 animals c) AHH will be performed 6 hours post final exposure: 10/5, 10/13. d) Serum Clinical Chemistry/Hematology samples will be analyzed as folloe: 9/14 - 12 animals ~ 9/22 - 12 animals tp 10/5 - 24 animals ~ 10/6 -- 12 animal s ~ 10/13 - 24 animals ~ OD continued A UNIT OF~hittalker C O  0 0 /1 ••. 0 M

Microbiological Associates DATE: August 10, 1983 TO: D. Dansie, C. Dove, S. Gosnell, WC Hall, C. McKinney, A. Reiter. LOCATION: FROM: RD Curren, RM David, CJ Henry LOCATION: SUgJECT: Schedule for Inhalation Studies Comments (cont.) e) Lavage fluid enzyme analysis will be as follows: 9/14 - 12 samples 10/6 - 12 samples f) Lung weights will not be obtained from lavage animals: lung weights will be from rats for mitotic index. g) Metophane will be used for all sacrifices. h) Colchicine will be given ip for mitotic index on 10/5 and 10/13, (12 animals each). 2 A UNIT OF~li>1ttSltel~ C O A• 0 A••- 0 M