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S6 12 PROPYLENE(W)GLYCOL^ 6/7/1 0107218 IDENTIFYING NO.: iR01CA38131-01 AGENCY CODE: HHSNI Glyceryl monooleate and pancreatic carcinogenesis (rats) PRINCIPAL INVESTIGATOR: LONGNECFtER, DANIEL S DARTMOUTH MEDICAL SCHOOL DEPT OF PATHOLOGY HANOVER, N H 03756 PERFORMING ORG.: DARTMOUTH COLLEGE, HANOVER, NEW HAMPSHIRE SPONSORING ORG.:NATIONAL CANCER INSTITUTE FY : 84 Glyceryl monooleate and pancreatic carcinogeriesis (rats) 6/7/9 0086677 IDENTIFYING NO.: 0045547; 6435-20523-008D AGENCY CODE: AGR DETERMINATION OF RELATIONSHIP OF STEREOSPECIFIC TRIGLYCERIDES OF PEANUT OIL TO ATHEROGENICITY PRINCIPAL INVESTIGATOR: WHITE F L SR ASSOCIATE INVESTIGATORS: PARRISH F W; BAILEY A V PERFORMING ORG.: SOUTHERN REGIONAL RES CENTER, SOUTHERN REGIONAL RES CENTER, NEW ORLEANS, LOUISIANA 70179 SPONSORING ORG.:U. S. DEPARTMENT OF AGRICULTURE,AGRICULTURAL RESEARCH SERVICE 790B13 TO 840813 OBJECTIVE: Synthesize certain stereospecific triglycerides of dual-labeled fatty acids and relate glyceride structure to the high atherogenicity of peanut oil. APPROACH: Develop procedures for synthesizing sizable amounts of stereospecific triglycerides identical with both those believed to cause the relatively marked atherogenicity of peanut oil and those believed to be innocuous. Develop procedures suitable for the preparation of sizable quantities of oleic, linoleic, and behenic acids, each labeled at two levels With a stable iostope (carbon-13 or deuterium) and prepare each of the labeled fatty acids in pure form. Synthesize selected stereospeific triglycerides of the dual-labeled fatty acids, use these in short-term feeding tests with animals, and establish the relative proportions of thelabeled fatty acids among the lipid components of the circulatory system.. PROGRESS: Work has continued on the preparation of the many intermediate compounds necessary for the synthesis of peanut oil-type stereospecific triglycerides containing dual-labeled oleic, linoleic, and behenic acids, and exploratory runs have been carried out on the procedures to be used in preparing the triglycerides. Batch quantities of the diketo acids of 'oleic and erucic acid were prepared, deuterated, and reduced to the dihydroxy acids for use in preparing oleic acid-8,8,11,11-d4, behenic acid-12,12,15,15-d4, and behenic acid-12,12,1„ 14,15,15-d6. Behenic acid-13,14-d(2) was prepared by deuteration of erucic acid, and used in thepreparation of 3--behenoyl-13,14-d(2)-sn-glycerol, a starting material for some of the triglyceride syntheses. Propylene glycol monobenzoate was prepared and reacted with triphenylphosphine in EFi~e-presence of HBr togive the triphenyl phosphonium halide salt, which is an intermediate inthe preparation of labeled linoleic acid via Wittig coupling with deuterated hexanal and azaaldehyde. (78698242 10