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(/l/~ A Division of Loews Theatres, Inc., Research Center, 420 English Street, P.O. Box 21688, Greensboro, North Carolina 27420 May 16, 1984 Dr. Carol J. Henry Microbiological Associates 5221 River Road Bethesda, MD 20816 Dear Dr. Henry: We have the following comments and'questions concerning MA Experiment No. 1-2130.005, Six Week Repeated Smoke Inhalation in Mice to Compare Histopathology and Short-Term Endpoints Using Test (D13 and D17) and Reference Cigarettes, Draft Final Report: 1. On page 9, paragraph 3, line 4: either the word "approxi- mately" or the symbol "' preceding "130" should be deleted'. 2. On page 11, under Blood Collection: a. line 3 - the text should read "0.3 mL of blood." b. line 5 - the second'"K-EDTA" is improperly spaced. 3. On page 13, under Evaluation of Test Results, paragraph 3, line 9: the text should read "statistics were used'." 4. On page 15, paragraph 2: a. line 13 - the text should read "but was within." b. line 20 - the text should read "Final Report (1-1729.003)), provided." 5. On page 15, paragraph 3, line 5: the text should read "remaining 9 animals." 6. On page 16, second complete paragraph, line 10: the text should read "Figure 1). Mice exposed." 7. On page 16, last line: lungs should be plural. 8. On page 18, paragraph l: there is no reference to Tables CCHB-CCH30, or to CCH38-CCH60. 9. On page 18, paragraph 2, line 8': "sham-exposed" should be hyphenated.

Dr. Carol J. Henry Pa e 2 g May 16, 1984 10. On page 18, paragraph 3: a. line 3 - observed is misspelled. b. lines (RBC) 3-4 - the text should read "Erythrocyte counts were significantly decreased." 11. On page 19: a. paragraph 4, line 3 - observed is misspelled. b. paragraph 5, line 2 - the text should read "slight but significant decrease." 12. On page 19, hyphens should be added in the following places: 3. a. paragraph 1, line 3-"smoke-exposed!." b. paragraph 3, line 6-"smoke-exposed'." c. last paragraph, lines 5 and 12 - "smoked-exposed," and in line 1 - "smoke-related." In Figure 1, at the bottom: please add to the footnote "Significantly Different than Sham-Exposed Controls." 14. In Table GT1: a. Should the first animal number be "1609"? b. Should the "Group" be specified next to "CIG," i.e., REF, D13, D17, SHAM? c. Should the superscript "a" be on all of the "Missing" notations? d. A footnote should be added for animal number 1642, stating that it died'on Day 35. 15. In Table GT4, footnote "a" at the bottom: please add' "Significantly Different Than Sham-Exposed Controls." 16. In Table GT11, the superscript "a" should appear next to "SD" in the Table instead of "Weeks." 17. In Table GT12, "(pmol/min/mg protein)" should be placed under "Tissue AHH Values ± SD." 18. In the following Tables, a superscript "a°' should either be placed in the table as needed, or removed from the footnote at the bottom: Table CCH1, CCH2, CCH3', CCH4, CCH6, and CCH36.

Dr. Carol J. Henry Page 3 May 16, 1984 19. In Table CCH54, footnote "a" at the bottom: please add "Significantly Different Than Sham-Exposed Controls." 20. In Table 2, last page: the word'microscopic in the title is misspelled. If you have any questions, please feel free to call. Since~ely, - ~~ ~ Connie J:'Stone, Ph.D., D.A.B.T. /tb:1