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~ Co7lea gue::17 '". Thts~ssto noti fy you thatthe Division of Lung Diseas sis, su somrg a`tiiork . r ;i ti ph d a Mo l of the lu b h h 12 i930 1 r ~ : ta r -, , s I op Goq Qa r n9 to~ e ~1 ve o ogy e) rc at :M t H rd 19 dy B tbs _~~ *' l h l t h r nrya e assac use ; ~ ,,, ca $c oo " os on ~ r' #s~ ~` `~~ ~ . ~ ~~t~~c ~~~'A~~a ~'+~k ~ i7 uq ~ ~ ~ ~ x ~ . scfthe ivorkshop aretoproyidea;theoretical and praet3cal coa-Orehen-": -_ sive rev~ew~ofbasic~stereoloyic"-concepts and~thetr.application to "~iulrno,iary'-~ ies areh for those currently engaged in incorporating"morp5oinetnc't._chnio ues , nto their basic and clinical investigat:."ions'l; Ilk Y{xi_y As you.r y be awzre_the Division of Lung-Diseases periodical7y sponsors=s7orY shop~,"i:'~zi c~ `participants `can'receive: practical • zs, v:zll as-thqorztica i exposure to a~ eas of l ung cel l bi ol ogy that are i n rapi d, dzvel opiment and reF reseiit' po~ 2r- ". I ful reseerch tools for fUrthering our "understand~:~g of luny d;sees2s Studies on microst'ructure.of the lung have continued toadvance aloneside tecF:= r:ological devel'opments, particularly in the area of .electron miicroscopy.'; ilany approaches, hos•rever,-have been purely qualitative.'and biological:samplCs and technologicali resources`havenot been utii.ized'to their;fiullest`2xtent ~- " ` t 'either basic or~in.clinical investigations,, Becausesuf`iciert Experience _ ' r e o i r a e ' _ s now available to in oul ona y_st eology i pply techniau s on a pra ;natic= - basis"ive feelthat there: is' a need' for a warkshop where investie:ators who are - -planning to,employ such techniqiies can discuss th,zn with a faculiy_of e,perts and receive"hands-on" experlence: - Ariraft of thP .ici-ksho?_agenoais eoclose:.' . ~epp toge•thr with irstrnctions'on he•r to aplyfcr _ a If Yau_.have any tiuest o~s resard~ogtne workshop, please contact D_r. Stripp, 1•:ho_ is,ihe ceo;dinator.` Her telephone rwmber is 301-49b-7171 Cc, ...~ ncere , y yours Claude tenfant, M.D~ _ Director ' Division of Lunguisea:.es '' l

DIVISION OF LUNG DfSEASES WQRKSHOP ON QUANTITATIVE MORPHOLOGY OF THE LUNG importance of unbiased sample mathematical adjustment` of data' to bias introduced by two-dimensiional i 1 • Afternoorn surface density - determination of relative volume and surface area - choice of fixation technique x - inflation pressure in.relatiori to `-physiological parameters - adjustment for shrinkage ' Practical Sessi'ons. . Determination of the size distriibutioni of a population of cells- alveolar (a) Determination of the relative volume of a lung tissue_compartment by poin•t counti.ng on 1'ight micrographs. (b)' Determiination of the relative surface area of a lung boundary membrane by intersection counting on llight micrographs. 3. Determination of mean linear intercept of alveolar surfaces'on liight micrographs, 4. (a) Measurement of lung voliume by water dii~splacernent (b) Measurement of tissue shrinkage after (i) fixation ( i ii) process ing and embedd iing. macrophages obtained by bronchopulmonary.lavage. Stereologic principles: _

Using the data derived in 12, 3, and 4, obtain absolut~e values for lung compartmental volumes and surface areas. March 13 '~ -"Morn~n _ - 1 Appl~cation of 2. Measurement of Conferences electron microscopy in quantitative morphology numbers per unit length - per unit area ~ per unit volume ^ problems of size and shape factors Interpretation of cell biological data _ ~. _ . rregularities'associated with various lung diiseases - Quantitation of autoradiographic images absolute values. by point and intersection counting on electron micrographs. Derivation of `Afternoon'~ . Determination of volume and surface densities of subcellular compartments chyma using the data derived i'n, (b). Derivation of number per lung using the values of lung volume determined'on Day l.- (c1 Determination of the number of alveoli per unit volume of lung paren- __ i (a) Measurement of number of gobl!et cells per unit length of tracheal wall. _ (bZ.-Measurement of the number of alveoli per unit area of lung parenchyma." 3. Autoradiographic measurements on electron micrographs. Determination of morphometric parameters on pulmonary arteries in light and electron micrographs. Qracti.cal: 1Z Morphometric techniques applied to rigidly oriented structures such as airways and'ivasculature - Conference: 2) Technology as an aid in quantirtationi Q

t + Practical: Demons trat iom of currently available morphometric equipment. (a) - automated sanipling stage mircoscope (b) -semi-automatic digitizers (c) simple image analyzers - Workshop will finish in the early afternoon. Faculty Gary Huber, M.D. Chairman Lynne Reid, M.D. Co-chairman Paul Davies,`M.D. = Organizer - Robert Bo7ender, M D James Crapo, M.D. ' Peter Gehr, M.D:. ~ . .. 4' Joan Gil, M.D. Gloria Massaro, M.D. Barbara Meyrick, Ph.D: William Thurlbeck, M.D. Institution Harvard Medical School Harvard Medieal'School .Harvard Medical School; .` University of ldashington. "Duke University Anatomisches „ lnstitut der Universit~at Bern, Switzerland- University, of Pennsylvania University of M'iami Harvard Medical School University of Manitoba w b-+ ftl~

WORKSHOP ON QUANTITATIVE MORPHOLOGY OF_THE LUNG SPONSORED BY THE DIVISION OF LUNG DISEASES INSTRUCTIONS March 12 - 14, 1980 _ - HARVARD MEDICAL SCHOOL Who Should Attend?: This workshop i's designed for 'investiigators in the applicant per program will be selected. ~' researah programs supported by the Division of Lung Diseases. Only one :'Lung Diseases..- First priori'ty.wiill be given to project directors of in utilizing morphometric techniques as part of their ongoing research efforts: Since the course can accommodate a limited', number_of partiei'l- • pants (25), 'seTectiion of participants will be made by the Division of United States'who are conducting studies on the lung and are interested •Cost: ' Cost of transportation (economy round-fare to Boston),,lodgi'ng at the Gardner House, and fees associated with Lhe workshop will be provided by the Division of Lung Diseases through arrangements wi,th Harvard Medical School. ` How to Apply: The attached application must be submitted to Dr. Stripp at the Diivisioni of Lung Diseases no tater thaniFebruary 1, 1380 at the following address: Bitten Strippy Ph.D. Chief, Structure & Function Branch Division of Lung,Diseases ,5333 Westbard Avenue - Room 6A10 Bethesda, Hd. 20205 Participants will be notified of t~heir acceptance to the workshop by 1980. - February 21,

WORKSHOP ON QUANT!ITATIVE MORPHOLOGY OF THE LUNG SPONSORED,BY THE DIVISION OF LUNG DISEASES March 12 - 14, 1980 -.~- _ _ _ . . . .. . . . , ~ ... . _ .- - . . '- APPLICATION . _, ., Name:„ Soc. Sec. $ Pos i t i on: Telephone - Work HDme Employment Address: List Highest Degrees and Postdoctoral Training Institution Degree Year Awarded a 0 List Current Grant Support with the National Heart, Lung, and Blood Instiltute Grant Number Project Period On the reverse side describe your previous experience with morpholiogic techniques, and provide a detailed', explanation onlwhy andihow participation in this workshop will help you better pursue the objectives ofi your current and planned research program.