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, (BIO-RESEARCH CONSULTANTS, ix O C4N.S LLLTA.T-16~-- RESEARCH • DEVELOPMENT F. HOMBURGER, M. D. P. BERNFELD. Ph.D. 9 COMMERCIAL AVENUE CAMBRIDGE II, MASSACHUSETTS UNwsrsiy A -B73S July 24th 1964 U \~ n Dr. H. B. Parmele, Vice President P. Lorillard Company 200 East 42nd Street New York, N. Y. Dear Dr. Parmele: Before leaving on vacation I should like to report to you briefly on the various experiments now under way. 1) Long-term painting experiment C-131R. We are enclosing three graphs which show that a) the percentage of papillomas in mice being painted with tar B2 have now reached 95%, and we should like to suggest that this group be now sacrificed. b) From the curves of B2 and B10 we may conclude that there is an active co-carcinogen in this material, since the effect of increasing the dose was to cause the tumors to appear sooner without a change in the slope of the curve of the cumulative tumor incidence. c) The comparison between the Pall Mall, Kent and Winston tars has reached a point between 50 and 70% of papillomas, and we should like to suggest that this be continued until the middle of September, at which time this experiment should be discussed between us. The same is true of the curves on the K75 and K12 tars. 2) Experiment C-151 (DDD). I should like to report that the experiment is proceeding as planned, and injections have been made July 15th. . . • bcd1- 3) C-140R, a co-carcinogen skin painting study proceeded well. On July 8th an accident due to collapse of a cage rack beyond our control occurred which '•N reduced the population of groups 24, 25 and 26 and their subgroups. In some ^ `~ of these groups this reduction was only 30% but in others the loss was greater. In order to remedy this situation we immediately instituted new groups 24, 25 and 26 and corresponding subgroups, which was possible because we were able to order mice of the same sex and age as those originally used, and O we shall add these groups to the experiment in approximately two weeks. ~ It should be noted that the new groups include new simultaneous positive Ir and negative control groups. ~ t /7 VY

,*1 Dr. H. B. Parmele -2- July 24, 1964 This will mean for some groups which were involved in the accident there will be considerably more animals than originally planned and for others the number will be at least that originally planned. The final results will be delayed by approximately eight weeks, a lag period which we shall carry at our own expense. C-146 Polonium experiment. Following up on Dr. Kelley's memorandum I should like to report that with the smoking terminated, the counting is proceeding satisfactorily and as scheduled. During my absence during the month of August Dr. Bernfeld will be in full charge of all Lorillard experiments and will keep close supervision of all matters concerned. He is aware of the fact that you like to be currently informed on the studies on DDD and will let you know if anything striking will happen. Sincerely yours, FH=,bhn Freddy Hor-Aurger, M. D. encl.

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