Anne Landman's Collection
THE Physiological and Biochemical Responses to Cigarette Smoke and Cigarette Smoke Components Ii. the Insignificance of Cell Death in the Depressed Growth of Yeast Cells Exposed to Whole Smoke.
Abstract
Reports on a scientific test done on yeast to explore the "cell death response to smoke." Notes that cell death "is not an important factor." Notes that cells go into respiratory arrest when exposed to cigarette smoke.
Fields
- Author
- *Ikeda, R. M. (use Ikeda, Robert M., Dr.)Associate Principal
- Neal, W.K. Ii
- Walsh, D.T.
- Neal, W.K. Ii
- Recipient
- Dawson, R.F.
- Daylor, F.L.
- DeBardeleben, J F.
Research Professional- Eichorn, P.A.
- Fagan, R.
- Hind, J. D. (PM)
Principals- Hoelzel, C.B.
- *Ikeda, R. M. (use Ikeda, Robert M., Dr.)
Associate Principal- Jenkins, R.W.
- Kosakowski, B.J.
- Laszlo, T. S.
Principals- Lowitz, D. A.
Manager- Merritt, H.B.
- Meyer, L.F.
- Mutter, W.
- Neal, W.K. Ii
- Osdene, T.S.
- Osmalov, Jerome "Jerry" S. (Joined PM in Dec. 1964 to work on filter development)
- Rainer, N.B.
- Resnik, F.E.
- *Teng, D. M. (use Teng, Daniel M., Ph.D.)
Research Professional- Thomson, R.N.
- Wakeham, H.
- Walsh, D.T.
- Weissbecker, L.
- Will, F. III
- Carpenter, R.D.
- Daylor, F.L.
- Named Person
- Neal, W.K. Ii
- Named Organization
- Fleischman
- Litigation
- Stmn/Produced
- Stmn/Selected
- Type
- Bibliography
- Chart/Graph
- Scrt, Scientific Report
- Chart/Graph
- Subject
- biological function
- biologically active substance
- research activity
- research studies
- biologically active substance
Document Images
TABLE II
(5393/38)
V~IABILITY~ COUNT OF YEAST CULTURE BEFORE SM0K~INJG (CELL COUNT X 10~s)
C
LIVE CONTROL EXP,
DEAD % DEAD LIVE DEAD
% DEAD
118' 0 _0.00 118 0 0.00
112 0~ 01.00~ 1~101 0~ 01.00
74 1 1.35 108'~ 0l ~ 0~.00~
B, V' I AB I L ITY COU NT OF YEAST CU LTUiRE AFTER 20 f 111H.
114
105 1 0. 818 112 0
0 0.00 122 1 0. 010
0.83
132 0 0.00 114 2 1.75
C, VIAB'ILITY' COUNT OF YEAST CUL'TURE APPROX. 2 N'RS, AFTER SNIOKI NG1
179 0 0.00' 94 0 0.00
164
2'04 0
1 0. 00,
0.015 102
92 0
0 0. 00
0.00'
N
O
O
O
W
C1T
O
~ C.0
c
TABLE DII
VIABILITYCOUNT OF 2% ETSS YEAST EXPOSED TOA DOUBLE DOSE
OF WHOLE Sh1QKE FOR20 MIN, (CELL COUNT X 105) (5393/41)
l! IVE CONTROL
DEAD
% DEAD
LIVE EXP.
DEAD
% DEAD
143 3 2.10 12'6 2 1.59
115 0 0.00 140 6 4.29
137 0 0.00 146 2 1.37
\ TABLE IV
V IAB I LI I TY COUNTS OF 10% SS YEAST EXPOSED TO WHO LE SMOKE
(CELL COUNT X 10s )
EXP'ERIMENT CONTROL EXPERIM'ENTAL
53:9s/43 LIVE DEAD: % DEAD LIVE DEAD~ % DEAD
166 8 4.82 114 3 2.63
i55 6 1.87 99 4 4.04'
144 10 7.95~ 112 1 0.89
5393/45~ 91 5 5.49 72 0 0.00
97 6 6.69 91 5 5.50
96 13 13.55 101 2 1.98'
C
~
N
O
O
O
Co
Ct1
O
N
O
Figure 1. Staining procedure in preparation for counting
live vs. dead yeast ceils.*
treat-allow 20 minute
effect period, remove
5-m1 sample aseptically
spin down (clinical
centrifuge) for 3 minu~tes
1 -
Supernatant Pellet
(Discard~)
wash with~ distilled H~z0
spin doti,n for 3 minutes
Supernatant Pellet
(Discard) I
add 5 ml sta'in
0.02'.~ methylene blue for
5 minutes
spin down for 3 minutes
Supernatant P'ellet
(Discard) I
5 ml distilled HZD
Count on
Heamacytometer
* IModified from Townsend and Lindeg,ren (4) .
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