Tobacco Documents Online

HT®012U1.i9 10. Henry, C.J., Caton, J.E., Stokely, J.R., Guerin, M.R., Lopez. A., Avery, M.D.. Dansie, D.R., Henderson G.M., Gayle, T. Whitmire, C.E., Kouri, R.E. Deposition and Distribution of the Total Particulate Matter of Cigarette Smoke in Mice using a Large Capacity Smoke Exposure System. Toxicol. Appl.Pharmacol. (in press) (1981). 11. Henry, C.J., Lopez, A., Dansie, D.R., Avery, M.D.. Whitmire, C.E., Caton, J.E., Stokely, J.R. Guerin, M•R., Curren, R.D., Kouri, R.E. Distribution and Clearance of Three Cigarette Smoke Constituents, Dotriacontane (DTC), Nicotine (NIC), and Benzo(a)oyrene (BP), after Exposure of Mice to Whole Cigarette Smoke. The Toxicologist. 1(1981) 139. 12. Henry, C.J., Whitmire, C.E., Lopez, A., Dansie, D.R., Avery, M.D., Caton, J.E., Stokely, J.R. Holmberg, R.W. Guerin, M.R., Kouri, R.E. The Dosimetry and Distribution of Whole Cigarette Smoke Particulates in Inbred Strains of Mice: Comparison of a Large Smoke-Exposure Machine (SEM) with a Small-Capacity Smoke-Exposure Machine (Walton). In: Pulmonary Toxicology of Respirable Particles, C.L. Sanders, F.T. Cross, G.E. Dagle and J.A. Mahafley, Eds. Technical Information Center, U.S. Department of Energy, NTIS, 1980, 177-192. 13. Higgins, C.E., Gayle, T.M., Stokely, J.R. Sensor for Detection of Tobacco Smoke Particulates in Inhalation Expo- sure System. Beitr. Tabakforsch. 9(1.978) 185. 14. Holmberg, R.W. Determination of Particle Size in Tobacco Smoke Inhalation Devices Using ::=thylcyanoacrylate Fixation and Scanning Microscopy. In: Tobacco Smoke Inhalation Bio- assay Chemistry. M.R. Guerin, J.R. Stokely, and C.E. Higgins, Eds., DOE Report ORN'L-5422, Oak Ridge Natic•nal Laboratory. NTIS, 1979. 15. Hwang, K.K., Bhooshan, B., Kouri, R.E., Henry, C.J. Synthesis of Tritium Labelled Catechol. J. of Labelled Compd. and Radiopharmaceuticals. (in press) 1981. 16. Bwang, K.K., Sonko, 0., Dansie D.R., Kouri,. R.E.. Henry , C.J. Studies in the Deposition and Distribution of Catechol from Whole Cigarette Smoke in SC3F1/Cum Mice After Exposure to Smoke. In preparation. 1981. 17. Kay, K.R. The Collision vebuliz=- Description, Perfor- mance, and Application. J. Aerosol Sci. 4 (1973) 235. 18. Kouri, R E. Genetic Differences in Chemical Carcinogene•sis. CRC Press, Boca Raton, Fla. 1980. 10 ~'f ' .`IiCrOltemlOgiCa1 .~' l"~ .W~°~

.._~.~r ~ . ...._.-._.. ~u}..r^.~ _Y 111 h01 2GG20 C 19. Kouri, R.E., Nebert. D W. Genetic Regulation of Suscepti- bility to Polycycl.ic-hydrocarbon-induceC Tumors in the Mouse. in: Origins of Human Cancer Book A, Hiatt, H H.. Watson, J.D., and Winsten, J.A., Eds., Cold Spring aarbor Laboratory, New York, 1977, 811. 20. Kouri, R.E.. Rude, T.H., Curren, R. D., Brandt, K. R. , Sosnowski, R.G., Schechtman, L.M., Benedict, W.F., Henry, C.J. Biologic:ai Activity of Tobacco Smoke and Tobacco Smoke- Related Chemicals. Environm. Blth. Perspect. 29 (1979) 63. 21. Kouri, R.E., Schechtman. L.M., Nebert, D.W. Genetic Control of Carcinogen Metabolism. In: Genetic Differences in Chemical Carcinogenesis. R.E. Kouri, Ed., CRC Press, Boca Raton, Fla., 1980,21-66. 22. Moneyhun, J.H., Stokely, J.R., Florant, L. Process and Instruments Corporation Automatic Smoke Exposure Machine -- SEM II. In: Tobacco Smoke Inhalation Bioassay Chemistry . M.R. Guerin, J.R. Stokely, and C.E. Higgins, Eds., DOE Report ORNL-5422, Oak Ridge National Laboratory, NTIS, 1979. 23. Parker, J.C. The Possibilities and Limitations of Virus Control in Laboratory Animals. In; 7th ICLAS Symposium, Utrecht 1979, Gustav Fischer Verlag, Stuttgart, .Tew York. 1980. 162-172. 24. Rasmussen, R.E., Boyd C.H., Dansie, D.R., Kouri, R.E. Henry, C.J. DNA Replication and Unscheduled DNA Synthesis in Lungs of Mice Exposed to Cigarette Smoke. Cancer Res. (In press) (1981). 25. Verma, A.K.. Shapasl B.F., Rice H.M , Boutwell R.K Correlation of the inhibition by Retino:,ds of Tumor Promoter-Induced Mouse Epidermal Ornithine Decarboxylase Activity and of Skin Tumor Promotion. Can. Res. 39 (1979) 419. 11 )C__- .1tiCiobiO:AsSOCib.

1-• i-. Figure 1. STAGES IN CARCINOGENESIS . ('.fM .AfiCPK~GENE9iS ~- INITIATION somommmo norw~r~a~ son HYnn.wry R.E. Kouri, C.J. Henry, , ., >+--BIOCB-IEMICAL PROMOTION-- A-% CELLULAR PROMOTION FACTaRS N TllMOii GF'iOVVrH .a•aaE staos pens4a Ym...CaWlancw inLe.wpp..won ~ FACTOM N CELL PROI.RRA170N E~ooaer.ous ew W.moo newwoa p`9 LxOCfnOUS M•41M fAsmL W

HT®0120022 \ \ Figure 2. SEM II animal containment system. One side of the unit is shown, with ~he capacity for exposing 240 mice per side (480 mice total) Rubber stoppers are used in the exposure holes when less than the full capacity of mice are to be exposed. ~a .*aadaww~C~ ~

( .( Figuse 2. H TQ01 20023 ~

4 Figure 3a,b. Animal restraint system, showing "stock-like H"T®0120024 r holder.

Figure 3a. ° ~~aausrmetirsLoar Fi;ure 3b.

HT®0120026 Figure 4. The SEM II with front and side panels open to show electronic componets. Cigarettes (C) are loaded from a hopper (EO) into a rotating drum holder (DH). The lighter (L) automatically ignites the cigarette and puff air is forced through each cigarette in turn. Flow is caused by a constantly held differential pressure in the dome (DO) between the ignited end and the btitt end of the cigarettes. Dilution air (DA) is introduced at the butt end of the cigarettes. Side stream smoke is removed through the vent (V). Ciga- ettes are removed by the automatic ejector (E) and discarded in the butt chute (S).

( Fig. 5(a) Wsiton Hoeisontal Smoideg %IaehEae. Mice• ue shoaa teeQaiaed in the cyiindttcai eube& ~

t Fig. 5(b) Waltaa expoetue eh-uaber. One sde plate haa been temo.ed to show the inside bt espwuse chamber tCH) with the canft¢Uyhaped exposuse holes (Zi) visible. Dunag an eaposute the dgaeette- dome (p) moves foraraed and eutomaticallq igmtes the dgetens IC). and 35 ml of puff air in fereed through the ciguette into the- chamber. A contiouous+Iq rotating [an (F) is t*qnired to enmssea rapid and unifotse mixiug of the smoke. At thb end of an eslosuae iatetval, smoke is vented through the eshamt port (E). H 11 I ...'r11 •r ~U~~r L.9