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i.,tu ~ulrrllriltau.u t aut q:t wu ~gcaa • . , - b Official Abstract Forln H T~ 0119 2 3 2 •wa I l .\1I e~01111 S AND SURCIUSSIF'R ATIONS (FW In ® one eateaory and on. eub elaairk.tlan onN 1 02 03 IM 05 Or, 071 ® Ie 11 12 u la Is THE EFFECT OF LIFETIME EXPOSURE TO WHOLE CIGARETTE SMOKE IN BC3F1/CUM MICE. C.J. Henry, L.B. Billups, W.C. Hall, M.D. Avery, D.R. Dansie, H.D. Mullinax, and R.E. Kouri, Microbiological Associates, Bethesda, PID 20816. Using the SEM IIB cigarette smoke exposure system, "nose- only" exposure of BC3F1/Cum mice to 2R1 cigarette smoke (high tar, high nicotine) resulted in deposition of 100-200 ug smoke particulates/mouse/day with '%,40z of the deposition in the respiratory tract. These exposures resulted in increased: lung microsomal monooxygenases and ornithine decarboxylase, lung DNA synthesis, lung protein, lung/body weight ratios, and numbers of sister chromatid exchanges in bone marrow. BC3F1/ Cum mice were free of 11 adventitious agents and were shown to be a sensitive model for the chemical induction of lung car- cinomas (Cancer Rcs. 41, in press, 1981). Mice were exposed to smoke or slmm 5 days%week for 27 months. Over 1500 mice were evaluated. The only smoke induced lesion was an accumulation of pigmented alveolar macrophages. Incidence of spontaneous lung cancer (i.e., alveolar adenocarcinomas) was '-5%. Ex- posure to srnoke failed to alter this tumor incidence. Sur- vivors post 27 months (ti500) were allowed to live out their natural lifespan. No differences in probability of death from lung cancer were observed in smoke or sham exposed mice up to 36 months of age. Expojure to smoke in this model system re- sulted in significant changes in short-term endpoints, but no carcinogenic effects were noted. Whether these results are re- lated to the absence of particular viral pathogens or M co- ( nt Fr ul N D) Qe ns 06 PI~ lp asma pulmonis remains to be determined. Supported by The CouncLl for Tobacco Research-USA, Inc. PRESENTAr10N PREFERENfA (i'gl ia Mone box only): [] Shde ®Porter C]SdentifieFO.n a&iend6e Exh3it Due to Ihe aire and eomplealty of the proyram. lirul deddona reprding elide or postez.ariona 1YDL BE MADE AT r11E DISCRETION OF THE NATIONAL PROGRAM COMMITI'EE. AUTHOR IP7FORMATiON: Paper to be prescnted by Dr. Carol J. 11enrY .~~ S who ia the Phet author on Udr abstract OR Owho is e.ee of the auUwa of th4abatraet,but not the Iint suthor. Pleise tist the full name. address, and tetephone nu+nber of Ihe authorpreec nUng ehe papa, aad alro of the author to whom all conerpondenx relating to rhe abatrad should Ee wrt PreaentingAuthor: Dr. Carol J. Henry - Microbiological Associates OatrespondingAuthor: Dr. Carol J. Henry ® ir0 DM t'lfa~ slgnature of con,6tpooding Aulg Ahrtraelr not witab{e fot dircer photoreproduelbn aal be retyped at the nombul eost to rhe 3nnpond• I iag Author of S 10.001U.5. DOLLARS). 5 . ~ .,.~.