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1982 AACR ABSTRACT FORM f1EnDLINE FOR RECEIPT OF ABSTRACT IS DECEMBER 1, 1981 4''I'CR annual rmeeting is April 28-May 1, 1982 in SI Louis. Mrssourl• Thr unKmnl rypnl i npy ut Ihn AIIVT•IIAIT PIrKM tfnr phnron•prnJm hnn in IhrPn.t+vhu)s; nm.l Ik suhmdled alnnp with It phuuKnpIrs. Ihr ar.knnwl• rdgmr.nl rvrd. and 2 srlf•addrrssrrl. slamprd rnvr• Inprs (;ueslmns trylatrlrng prr•paratrun nf Ihis FIIRM .huuld hr dirrelyd Irr the Cancer Keseurch Feldorml ( If'ice. lrlrphonr: 21 S•?21•4720 " 1. ~,-i Ii 1- r,, Iq.l r rta' MI'ti'1' 11F, r\\ti\v1.RI:U m H Ikl. 1111 An% I KAr' 1 ( AV IIE la r.titilUER1,U 111' TI IF. I'KUI:RAM t.UMMI TI'F.F. tir.cmn In whu h p.qx•r mi4h1 In• .o h..lulwl p hi•- k nnc Iwe anlyi U nhmhmmwtrv 17 KndnKy and l:ennh.i; X l'arn miqp•nr•cis 17 l:h nu a I I nvncl i ealrnnc O Enducrmnluuy O EaprrimaMal Oremolherapy O lmmumlingy I 1 Vrrnlnpv I I'rescnlauwn pn'Ir.n•nn• mrr hnx uniyl. Irr• eouse ui the sae and crnmplrahy ul the Pnrylr.un. finrl decisions must be made s1 the discrelion of the Program Commrllec. ID Poslrv O Shdn OPTION: Olt the ahsttacl is not accepted lor presenralmn. the SPONSOR wishes to withdraw il from pubhealron inlhr.Proeeedings. DO NOT FOLD Typr abstract in blue epace below. See sample abslrecl on revene side. CICAkI:TTL• SMOKE INCREASE•S DNA REPLICATION IN LUNG AND SISTER CIIROMATID EXCHANGE (SCE) IN BONE MARROW OF MICE. K.K. Kanagalingam, A. Banerjee*, S.M. Recd, D.R. Dansie, W.F. Lenedict*, R.E. Kouri, and C.J. Henry. Microbiological Associates, Bethesda, MD 20816, and *Children's llospital of LA and USC School of Medicine, Los Angeles, CA 90027. BC3FI/Cum female mice werc exposed daily by "nose-only" inhalation to whole smoke from 2 reference cigarettes: 2R1 and 3A1, of varying tar/nicotine content. Under standard smoking conditions using the SEM IIB smoking machine, '6300 Ug total particulate matter was deposited/lung/day. We per- formed ].abelling Ind^x estimations :'n lung as an indicator of localized smoke-induceli effects, and studied SCE's in hone marrow as an indicator of systemic smoke-induced eifects. When mice were pulsed briefly with 31l-tllymidine immediately after the last smoke exposure, and the lung analyzed autoradiographically, DNA synthesis was found to have increased compared to controls after 9 weeks' exposure and Qrc increase persistcd for 52 weeks. Maximum response was found at 13 weeks, when thc percent labelled cclls were 'L2CX (2R1) and til4X (3A1) greater than controls. Labelling was confined to alveolar cells; none were found in hror.chi'. The majority (ti67X) of labelled cells contained less than 30 grains/nucleus suggesting that in addition to replicative D14A synthesis, repair synthesis occurred as well. Analysis of the bone marrow of mice revealed that 1, 4 or 46 week- exposure to smoke from either cigarette type resulted in N8 SCE's/cell compared to 'L4 for the unexposed or control sham- exposed mice. When the mice wF.re withdrawn from smoke ex- posure after L week or 46 weeks a significant increase in SCE's persisted for at least 1 week. Our data suggest that exposure to cigarette smoke causes elevations both In tey- midine incorporation (including unscheduled DNA synthesis) in lung cells and in SCE's in bone marrow cells. • Supported in part by contracts and grants from The Council for Tobacco Research-USA, Inc. 1-k•asr lul lull names and membership tnatus ol all aul hurlel mcludinp rhe SPONSOR and prerr•nter These should be in eaacny the aame onJer as rhry appur m the henkng of tlr •dqrrecl. • Nome Mrmlx rship Sratus Namr MrmbrrsMp stalut Ken K. Kanagalingam Member Carol J. Henry Member ^ ~ Ashutosh B®nerjee Non-member _5aI ~1M. Reed _Non-mgmb.e.>_ David R. Dansie __N_pn-llteID]t.gL ~ldilliam_ F. Benedict _Member Richard E, Kouri Member _ _ Ahdrau lo lia pn'sr•ml,l hy Ken-K. Kanagalingam _Mlcroblolopical Assoeiates ru pvl m~•.~t PRk'.L Iln.lihurml . 5221 River Rr+ad. Bethesda. P~aryTand 208NI1'F:NI fr+1493 (30]) 65y-~y~0 laddrra) (nry and elnlr, np ~ d.•. ~. r.mn 1 lalea anle• nMphunr numMr) \I1.1rar.1 SPUNSU'RED by Ken K. Kanagalingam Microbiological Associat_es _ 5221 River Road, Bethesda, 1&Fy~and`TbW61493 lin 1~.r. ) 654-3400 loddressl lcuv and slner rrp c.dr..unnlrvl lare, eah. lrkphune numb.rl Id who it an authnr of this obslr.Vt r,(T O,whn is "ml.udueing'• Ihie abstract the authurship of which is enkrcly nnnmunbcr. ti.Fuaturr or SPONSOR (1!1•0?AW 1'Jl.aso be certain that you ha e a red 4 all ICR abstract submission miles. Infraclions ot authorship regulations will result in tejectfon nnd return of the abstract to Ihe SPONSOR without consideration by Ihe Program Committee. Infraclioas of formal will result In a pennlly of 530 which will be billed to the SPONSOR.