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Ht®0119233 I ~ STUDIES ON THE DEPOSITION AND DISTRIEUTION OF CATECHOL FROM WHOLE CIGARETTE SMOKE IN BC3F1/CUM , MICE, K.K. Hwang, 0. Sonko, D.R. Dansie, R.E. KoLI, and C.J. Henry, Dept. of Experimental : Oncology, Nicrobiological Associates, 5221 River . Road, Bethesda, MD 20816-1493 Tritiated-catechol has been used to follow the pharmacokinetics and metabolic fate of inhaled catechol in cigarette smoke in BC3F1/Cum mice. The aresence of 3N-catechol in the smoke was verified by silica gel chromatography, and gas chromatography/mass spectrometry. Mice were ex- posed to 10% (v/v) 2R1 cigarette smoke on the Walton Horizontal Smoking Machine under standard . conditions of 35 ml puff volume, 2 seq/puff, 10 ~ puffs/cigarette. The depositifln and distribution of inhaled catechol were determined in all inter-' Dr. Kin-Kai Hwang Department of Experimental Oncology Microbiological Associates, Inc. 5221 River Read Bethesda, MD 20816-1493 nal tissues, urine and feces. Data showed that clearance was occurring during the 10 minute ~ (301)654-3400 smoke exposure period. Immediately after this exposcre period, 56X of the radioactivity was found in the blood, with 82 found in the lung, 12% in the respiratory tract, 13% in the liver, and 14% in the kidneys. By 120 minutes after exposure, less than 0.62 of the total dose was ~ X found in the lung and over 94% of the inhaled radioactivity was found in the urine. Less than lOT of these radioactive urinary aetabolites were extractable by chloroform-methanol (CM) or ethyl- acetate (EA) solutions. Upon treatment with 8- glucuronidase and arylsalfatase, at least 952 of ' the radioactive urinary metabolites were extract- ed by CM or EA solutionE. These organic extract- able fractions were purified, ana-lyzed, and three chemicals positively identif ied: catechol, hydroq,uinone and 4-methylcatechol. We conclude that catechol in smoke is rapidly absorbed, redistributed, conjugated, and excreted in urine from mice exposed to whole cigarette smoke. Supported by The Council for Tobacco Research -. USA, Inc... I • . x Inhalation; Industrial; Pulmonary