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---,.--~--- --~ ToBACico IxnvsTax HE3vA7`oCII Coht=Trx TIRC Grant #155R1. Duane G. Wenzel, Ph.D. University of Kansas I. "The Effect of Nicotine on the Therdnal. Circulation tndex, Blood Pressurer Blood Coegulation Time and Cardiac and Peripheral Pathology of Cholesterol- Fed Rabbits." II. "The Effect of Nicotine on the Blood Pressure, Blood Coagulation Time and Vascular (Heart) Brain and Periphery) of Atherosclerotic Rats." Report No. 3 March 10, 1959 I. Four groups of tvrelve female rabbits per grotap have beeai established as follows: Group 1: Control Group 2: 1 mg./Kg./day nicotine in drinking water Group 3: 1% cholesterol and 5% cottonseed oil in feed Group 4: Combined regimens of groups 2 and 3 All animals were approximately six weeks old at the initiation of the experiment. At this time and at four week intervals thereafter the thezma]l circulation index was detezmir,ed for each animal. The for- mula used was. T.C.I. Rectal temperature-skin temperature - --Skin temperature - room temperature Skin and rectal temperatures are obtained oy use of thermistor the=momete.ra having an accuracy of ± 0.01°C. Peripheral skin tempera- ture is d•qtermined on the shaved dorsal surfgee of the foot of the hindleg. The mean thermal circulation indices obtained to date along with their st€tndard errors are presented in Table I. The systolic blood pressures are measursd by means of a Grant ear capsule. The mean systolic pressures ± the standard errors are given in Table II. Of the original 48 animals six have already died. The deaths oc- curred in control 1, cholesterol 2, and nicotine-cholesterol 3. II. The second study to deteimine the effect of nicotine upon the athero- sclerotic rat has just been started. The groups in this study are es- sentially the same as with the rabbits except that male Sprague-Dawley strain rats are employed and the atherogenic stimulus consists of the following dietary additives: cholesterol 4%, cholic acid 1%, and thiouracil 0.5%. The rats are being started on this treatment at ap- proximately 100 Gms. wt. Determinations to be conducted include: blood pressure (tail), coagulation time, and the pathology of heart, brain, and periphery (hind legs). This Dacument Subject to 2 N K 13 7<3()73 Confidentiality Agreement

° _ -- A, '?P a.p o--~73Fa. .;Ay . . . ~,'sxn: y -2_ Table I Time in Week's Group 0 4 8 12 Control 4•35t•79 4•?S±•23 4•331'•25 4•37t•25 Nicotine 5•25T•41 4.0.31 4.10+.23 3•74t•16 Cholesterol 3.86±.43 4.99±.35 4.48t.26 4.46±.12 Nicotine-Cholesterol 5.81±.64 5.42t.37 4.15±.19 4.42±.35 Table II Control 72.00t.92 72.1617.48 74.83+.7ti 75•33±- 89 Nicotine 77.17±1.06 78.00±1.29 78.i7+1•3a 81.00t1.69 Cholesterol 78.00±.88 77.33tl .31 78.10'f1.06 79•27t•73 Nicotine-Cholesterol 76.17±1.22 76.17}l •22 78.20t1.o9 86.00t1.46 EXPENDrI'URE REPORT TO MARCH 1, 1959 eAIARIFS $1,158.00 rERtnNnaT EquIEIMENr 465 . 00 EXPENDIBLE EQUIPMENT 195.00 OVERJ= 1,005.00 TOTAL $2,823.00 This Document Subject to Confidentiality Agreement N K 1~:3 r')(174