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Z'HI: COUNCIL F(DI2 ToBAcco RESLAKCII-U.$.A., INC. February 8, 1977 MEMbRANDUM TO: Drs. Bing, Hockett, Jacobson, Sammers and Stone FROM: W.U. Gardner NKO??;?73~ri SUBJECT: Application 1138. Walter B. Essman, Ph.D., M.D., Queens College, Flushing, New York New Application No; 1138 "Correlates of Stress - Tobacco Smoke Interactions" The progress report relates the pl.asma eorticosterone changes in mice stressed by positioning in the cones and in the head holders with or without exposure to smoke in Walton. In the cone holders exposure to the smoke or air for 5 and 7 days (1 cigarette per day) lead to more than a doubling of the plasma levels of corticosterone. Daily exposure for 14 days resulted in a return to,basal levels. Mice exposed to smoke begin- ning at 20 weeks of age showed -io response to cone restraint or filtered smoke and much less response to whole smoke. Mice exposed to air or smoke in cone restraint showed a marked decrease in myocardial norepinephrine uptake in vitro. This, like the plasma corticosterone increase, tended to return to normal after 2 weeks. Two strains of mice showed a,significant increase in lung 5-hydroxytrypt- amine after exposure to smoke, the C57 mice did not. The significance of this observation is unknown. Skin 5-HT also increased upon exposure of mice to smoke. Uptake of 5-RT in vitro was also greater in smoked mice. Thase observations are being reported, a preprint was attached. A copy of a reprint of a paper published in 1974 was also attached and also a preprint of a section of a monograph. The research proposal is in 2 parts. One related to a continuation of the work on stress with increased interest on metabolic stressing experi- ences. The second relates to work on the monitoring of smoke-generated stress and adaptation. The mice chosen for the chronic expocure, BC-3F1, are to have adrenal weights taken, adrenal corticosterone levels determined, adrenal corticosterone sy nthesis done in vitro, and myocardial norepinephrine uptake determined. These determinations will be made in acute exposures and following prolonged exposures in the SEM machine. The metabolic siress studies will be done with CF-1 mice and Sprague-Dawley rats that will be raised in The Queen's vivarium. The laboratories are equipped for most of the work to be done except for a fluorescence spectrometer. Salaries, including summer salary for the applicant, make up a major item. Travel may be unduly large. WUG/mb I