Tobacco Documents Online

Tiih; (iOUNC1L rOR ToBAcco RI:SL•'ARCH-U.S.A., INC. July 14, 1976 MEMORANDUM TO: Drs. Hockett, Lynch, Sommers and Stone FROM: W. U. Gardner NYfin. I `t12 0 0 9 SUBJECT: App lication 1102. Harilyn A. Rasco, Ph.D., Assistant Professor of Biology, The University of Texas System Canoer Center, M.D. Anderson Hospital and Tumor Institute, Houston. "Hydrocarbon Metabolizing Enzymes and Lung Cancer." Although Dr. Resco is listed as principal investigator, Dr. Toshio YamAuchi is also project investigator. The latter writes the procedural outline and was trained in immunology, the former in biochemistry. They both have been working for about two years on the developinent and checking of methods of AHH determination and their studies to date are reviewed in the preprints and manuscripts submitted with the application. They have made three major changes in method and report their results in three parts. The percent of exclusions has decreased of data on individuals with each procedural modification. The patients with lung cancer have generallh had, as a qroup, higher AHEi values than non-cancer or other cancers with the exception of squamous celled cancer of the oropharynx. The distribution of AHH levels tends to be unimodal and probably polygenic. Actually they have no good genetic data. Proposal. They, plan to begin 3H--BaP method of assay. This will determine alll water soluble metabolites of the PAH used for inducer (not just 30H-SaP). This method is now used at Creighton. They will carry eight donors at periodic intervals to serve as standards and set iap an exchange with Creighton and MA that will exchange similar samples that will all be run on one time in the three laboratories and with similar materials. Second, they will continue the readings on lung and oropharyngeal cancers to increase the numbers of observations. Samples from these populations will be "index" numbers for a proposed family study of 30 in each group, controls and the two types of cancer. Age is important. The significant data were on persons utider 40 years. There was no difference between smokers and nonsmokers, stopped, or never smoked. The budget is for one technician and expendable supplies and for the first year includes a CO2 incubator, npproximately $2,000. Dr. Shaw has two other grar.tc totaling about $96,000: one for AHH and colon cancer, and one titled "Microsomal Enzyme-Studies." The budget seems reasonable. W.U.G. WUG:ek