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Council for Tobacco Research

the Role of the Macrophage in the Immune Response the Effect of Tobacco Products on Macrophage Function. [Research Progress Report Number 2]

Date: 19740601/R
Length: 27 pages
HK1713118-HK1713144
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snapshot_ctr HK1713118_3144

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Page 11: mlr1aa00
H. K M.1 , 1. 1) al 1-18 TAP,LL' 5 The P.ffect of :AA1.S on the In Vitro Anamnestic Antibody KesN;~nsc of Rabbit Popliteal Lymph Node Cells to K1,H Serum KLII NRS + CPi9 14 C-Lencine in Specific llntibody on Pav Time of T.x posure to AD1 S 30 min 2 hr 4 hr 1,635 1,238 1,255 17,339 16,751 13,917 1,128 900 814 6,289 3,735 2,372 t
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i N K®.l 71,3129 r PL71"•T. 1 The Cffect o` ?'?7, on thc `k)mholcrrv o` 'l?ase'Peritonczl '13cro~'~1~ ; in CsltuYe " -I Zero Tir2 of Incubatirn !tiiuse periton•eal exudate cells were cultured for 24 hours in supple^iented `E`4 as describa'3 in }•lethods. 7lfter the initial culture period, the plates «s.e washed and the adherent cells (^acrrnhac*es) were incublted for a total of 24 hours in *'~'f containinR either 20% FCS, 20% MS, or 20% of a specific A`LG. The cells sh:nan here o.ere exa.^ti.ned by phase contrast r.ticresconv, 150X ra~ciification, at zero tiine off i.emibation. A. Control cells incubated in FCS B. Control cells incubated in N2S C. }!Iacro:*,ages inculated in P: T, 100 (prea-Ared against ficoll- purifi2d r,iacrophages). L
Page 13: mlr1aa00
I H Ki~1 i1:~ t:~U y . .rt+ ty• 4 ,v a m , x + ~ . e n 0 . •r Y It -a
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NK117 l:3 131 1'LYTT_, 2 The Bffect o{ A'tS on the ~lorn'~lorn~ r,f '•ir~ase Peritoneal '+~cro~'.~~nes in Culture lifter 2 1/2 }]nurs of Thcubation !huse neritonenl macroplLlnes were incubated in 'iv^i cnntaininq either 20% FCS, 20z NpS, or 20g of• two d.-Lfferent and specific NtS for 2 1/2 hours. The cells we.re exTnLned by jplase contrast microscopy, 150X magnification. A. Control cells in FrS B. Control cells in ?,72S C. 1•'kicrophages in A^SS 98 (nrepxiced aaai.nst adherence-purified racropliages) D. t~acrophaaes in A'L5 iC-O (prepare3 a0ainst ficoll-purified macrop:-Iages) L J I
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H K®1'713133 r PLnrr. Tne DffeLt of on th^ 'bznholocny of 17ou.>e Periton:~al 'ticro-A~aes in Culture After 4 Hours of Tncubltion See legend for Plate 2 for details. A. Contz-ol cells in FC.S B. Contxol cells in 1'M C. AL3crop'-viges in P.'Lc 93 D. Macrop?mges in A*tS 100 L J
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t . ~ E ~^9. :; ~~ ---r • 0 {y • • Vti :•c ~ w t '•~ . r Pd ~ • . .j ~!Q _. ¢ { t 0 '. .•,f r ~ '~ { w .'i Y, ~w +. t 41 t - 4L s , • . ::- IIKI -•-•-,.._-.,. ~-~ - • . V• ' ~ •` G v
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H K V 17 13135 riNn: 4 The Eff.ect of TV'+S on the '4nrnho?c-v of "huse peritnneal "lacrnn'i.aces i.n '~ Ctitlture hfter 24 Hoars of Sncub~ttion See legend for Plate 2 for details. A. Control cells in FCS B. Control cells in P]RS C. 'dac:rophages in A*t5 98 D. Atacrop:-,ages in Ari^. 100
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NK®1713137 r I Ftctrn:' I Tho Efi ect of AMS Trr.atnent cn tho In Viro Primary, attd Decy 4 Sacondnry An:ibod; Re!:ponsn cif a.ica Lo O,frg Crudo Aeaociatod }LB m w f 512 128 32 16 8 Control AMS 97 L14S 99 Jl4S 100 I 4t 13 1 ~- 9 3 6 7 9 12 14 15 18 21 25 DAYS POST IMMUNIZATION (DAY 4. 2•) ( L -i I

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