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THE. Covlqar. FoR Tasecco RESEwRCH-U.S.A.., Ixc. N N11 1 59039 March 2, 1983 TO: Drs. Potatwell, Felc'bnan, Sato, Jacobson, So:mier's, HockqEt, Stone FbY+d, and Mr. Hoyt & Mr. Hobbs Pxxs W.U. Gardner Rs I. Bernard Weinstein, M.D., Oollege of Physicians & Surgeons, New York, NY First Aenewal Applicatian No. 1483R1 "Devrolopnent of Mornclonal Antibodies to Carciaogen-DNA Adducts" Progress - Most of the a9duats arose from binding at the 10 position of the BPDE-I and the rnanber 2 position an the deoxyguenosine. 11he modified LtvA was c.romplexed with methylated BSA and emulsified with fteunds' adjuvant and injected into tnice (Balb/cCr). 7he spleens were retlolrsd and fused with mouse myelana cells in polye- thylene glyool. Antibody activity wars tested by ELISA. Twu cells had the desired activity and these clones are now being used to get large quantities in agar for further studies. Other fusions are row being done to get other namclonal lines with different specificities. Projection - Studies will oontfnue on the dQA adducts. If they fail to have adequate sensitivity and specificity alternative approaches will be attenipt-ai3 as menti:ared in the original application. Zhey plan to clone 50 to 100 colonies of the subclones in agar and eheck for their stability, affinity and specificity. Budget - The PI might be asked to explain the need of the Apple II caqputer; Grunberger and Weinstein have 3 saae4hat similar NCI grants and a Hoffman IaAoche grant for vwrk on interferon. 7i,o applications are pendino. 7he budget might be examined by Staff. Ztao of the PI's co-investigators have turned over during the year. Oamrp.nt -7he group seems to be ooapetent to undertake the experiments. WUG/ff