Tobacco Documents Online

August 25, 1981 Tnr: COUNCIL Toit Tounc•cu Iir!•aLanct+-lJ.S.A., INC. rffrIow,NDUM f M: Drs. Bing, Fcrd, Hockkt/t., Jacobson, Sorrrnrrs and Stone FM1: W. U. Gardner 1ii(~rU:'~~Gr~~ £USTECT: Application No. 1223A Kjsll Ftir:e, Zaroli.nska Institute and Hor.mane Iaboratory, Karolinska Hospital, SWeden "Nioot.ine, Catecholamines, Neuropeptides and Neuroendccrine Functions" Progress-. Rats ovariectoanized for thirty days were given nicmtine lm3/Kg/4x at thirty minute intervals and killed thirty minutes after last injection showed inhi- bited prolactiui and IN but Tsh,GH and FSfI aere not lowered. This was inhibited by mecarrylamine, lmg/kg/2x. Male rats given 0.3 or 1.0mg nicotine/kg have been studied for the the localization of CAs (catecholamines) and pituitary hormone-levels. The observations reaffirm the previous studies with larger doses of nicotine. Intraven- tricular injections of as little as l0ug/kg in rats given a tyrosine hydroxylase in- hibitor showed a dose dependent reduction of DA and NA and increases in their turn- over was associated with lower serwn levels of TSH, prolactin 1.9 and LH atid increased FSH levels. 9noking acutely ieduced LlA levels and NA levels in discrete forebrain and hypothalamic sites, DA and NA nerve terminal systems (Dose dependent and signifi- cant after four cigarjettes.). 2R1 cigarettes were used; each puff eont.a.ins 0.24 mg nicot.;.ne and 1.55 an C0. The results to date show that srroke produces the same neuro- chemical changes as nicotine. Three preprints and cne reprint were sutynitted. Pronosal-. Subchronic exposures to nicotine or snmke will be done, that is exposures up to one and two weeks. Coaribined hist.ochemical, iJmnuiochanical and che- znical methods will be used to determine oantral monamine levels and turnover rates. ChroyLc niootine will be given by minipunps. Ehzytne inhibitors wi.ll be u_sed to sta- balize the turnover experi-nents. Progressively srnaller brain areas will be E.xamined as determined by additional observations. Studies will be done on acute and sub- chronic exposure to nicotine or anoke on enkephaliuis and substanoe P-like innnmorzdio- activity in different parts of the brain neuroendocrine tissues. The effects of nicotine on ;he beta-endorphins will be studied using the immviofluoresoence method. The pro- tocols are very open but he has used nost of the methods and pn,-edures or has eolla- borators who will supply materials or do certain analyses. Budqet-. About 5/7 for salaries. He is requesting $3,000.00 for a dehuni- difier. He mentioned this to SCS last sumner. He me.nticns only one other grant of $8,000.00 from the Swedish Zbbacco Council. Any rtodifications of the budget would be arbitary. Ooanrent-. It is difficult to deternvize just how much has been done to add to or to change basic concepts of nicotine effects on neuroe.ndocrine function. The Qeneral area is expanding rapidly as new rmdiators of function appear. T tkink that this is agood prxxMam and that two additional years is merit:ed. Rate high. W. U. Gp_-'ner k'UG : am