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dof i Carcinog.nesis 11 27e2 •erteaaoer 2StOR-ASSOCIdT9 AL)C=LtCTILLY:IItCL9 (A2J1G) Alt'J OS2-M 1.I7ID6 33 .1rE :•kT.23 .-L7I±G I.e.RALIA2:0lI-ITi(iCS'i :-E:,1T!C1.*_Rr3IS, !ober- C. lrovn. .. A. t':s:±v•, y. L. llanict ar.! Alezat:ra Ki:r:re• (asi.. of Sor:a Care:isa, Cbapel ala, J• i",_ .aa lisRe Aasoe. (ai.., Cai 3i3te. Tes. 3TL30. sed tutberford Paspital. Rutbertordtoc, y.C., 24.139 Ie aatiet;atica of locoliss•ty early ersaSea durf.te tae pre- dlietab:e i0-60 3ay ;eriod of post irrsdistios (post-y ) tttyale letaYSOResesis i•e tte CS'fl/t.f s,use, aaala:s of tuior-aawe- Sated aed otr.er :--SS a t~e :r:ysus at var:eus lnterva:e follovlaS •-voea:~~::: rad Soses of i-ra.s vaa oor,area with tboae is sinl:ar-aqe: eontrois taw vas eorre_atrJ vltt :r7ie alstolop. It cot:rast to eose o:ter :a.ors tcstlc leuas,ia eootalned ealy a very saall euaatity of AL:.O (c,T-1,CZ of ttau- trsl lipids coaaar.d to 0.2-0.35 for eeatrols). tloverer. Fn- t-L1-; results iasicated that levels ia )-day post{ tbysuses Mere elail.ar to t... te in ttyCle 1•eYae9;a. :~1e etner-lialea tA- tal pbosp,•u:lFiaa vere statlar in a.1 ;reu;s of post-/ atd coo- trol tb,vluses aa: :r.yslc auaemia. :aolesterol esters (CE) vert itcreasea ::t:,!n:e su.:eaia aaa 3-day post-,F tE7sntses asd tM latter a/so Sno.ed elerat.o ;eca: ea:es:.rol. Fatty scid distrikutios in CI in tGpe:: leusesia aad 3-day post-( tlpsuses were sL-i:ar :e ccr.tre:s. Prelialsary aaalyses seovea a decrease is :=:C, '.c:0• ind :t:l aad at :ncreue in 1ca arii 10:2 fatty aelds ia :riR-Yce::des in :a7s:c le•ueata coapared to controls. h- fa::y aeia : - _se`o::;iAS were slzilar :t tbyaie leuYeaia iac cer.tro:s. ('eseareb su;;c.^.ta by S:S :raac CA12222. U. S. A2C atd Aaericaa Caocer Assoc. araat BC-TCL.) 3203 wnrotoev TBE E`1DOCRI%T P.OLE OF OIETJIRY LI°TOS IN 41tM!'tJtRY U- NOR iOV~J.T:Q::. ;.Y. ~ass, Allaben• lrovn• 5 :u ar. , .e • oucaern :nols 1.c:sle , .ar~o- a.a. 6:7J1 Initial investlt?atit:n. which becan in 196t,shov- ed that dietary linids had an uterotrooie effeet. Further investlqatlon indieated that t.'+e effect oc- curred in rales also, i.e. increased serlnal veslcle weight. Cont:•^._e_ research into _ 3se effects :nda- cated that :-_-: itself was not direct2v resnonslble for this iecrcasc in uterine or ser•:nal vesicle wci- qht, since 1) a:l liolds ~roc:-jeed tie same effect whether saturated or unsaturatee, or of anlral or vegetable or:cln, and 2) castration and/or hypoonv- sectory b:ocre4 t.•.e effect of dletarv llo:ds in :ot1 sexes indleatl:+rf that an intact ottl:tary-qonadal axis was rec:a:et: and that the res-onsc vas in no vav related to the n-itr:tlor.al !ualttles of the diet. An exnerlnent was beqjn in which C3Ft fer.ale aice with the ril% :-:•+S:us .:ere olaced on dlets con- tainisq: no added linid: acded llol_ (butter fati: no added ::rl~ _t:: a~ded st:l:estrol: and a diet containing ad-de= 1:z:d and ae':ed st/li:estrol. The follov:-q :esu:ts :r•dlcate tne n,;-.ber of tumors in each dletarv c:ou=: no adt!ed :::td 1/21: 19.5% added lioid 6/2:: 257 ~oo added stllbestrol S/:S: :9.St added liotd o:_s 250 ne:n if added stllbcstrol 2/16. (Supoorted by ".e rracernal Order of Eaqles.) 2204 parMOt•omv EFF.CCS OF A::ERD DIETARY rAT I\:aKE ON SERLTt ^ROLAC:::1 '_-=.q. Leonard A. Conen• Frank Oldaco•, and Po Cauen C1an• (SPON: D.S. Reddy). Amerlcan Health Foundatlon, Nov Yotk, N.Y. 10021 'he mechanism(s) oy which high fat intake , enhances 0YB.t (7,12-dtmeth7lbenz(a)antr.raeene)- induced mar.aary ::umor deve:oprrsn: Sn rats was studied. Fe.:ale Spracue-Davlcy rats vere fed a semisynthetle diet c•.)ntalilnc 209 or 0.51 fat (lard or corn oil) fre- SOtn day of aqe. Ar:otaer group of rats was fcd Pur:na Lab C:c• tnrouqhout. Serum from the rats at the age of 110 and 200 days was assayed for -rr_lac:an vlt.`h a :12:.`l0 -RA'-PROLJ,CT:::- RIA kit. It :as found that at batn ttnc intervals rats on the hlch fat dict, lard :r corn oll, nad significantly nlqr;er scrun prolact:n :evels, particularly it estrus, t^an rats on tho low fat diets. 'hr se:•::n oralactlrn lct•al zetuoen rats fed the low fat ::ets :nd P•_ Sna Lao Chew were dlfferent -.:t ^ct s:=n:f:eantly. '.`•us, tne quantity of dletaryfat :atake, -.!aardless netner it is lard or corn oll, appctrs to affect prolactln synthesis and/or releaso. wur data suqqest that the elevated scrun prolactln titer is involved in the increased r..a.:rairy tumor Snc.eence in OM9A- treated rats fed a nigh fat dlct. (Supported ty LS ..:- ~;rant :.1 12376-03) par.ot•oor ALTERED INCORPORATfON OF ew-TMvMIDINE IN MA)SSTER TRACMEAL EPI- T1(ElIUM CAUSED !Y RESPIRA'ORY CARCINOGENS. Curtis C. warris. Davt ': K• n, i..reinoaeneses Program. Lung Cancer :rancn, t•ntSOa, Nd. 20014; roll+t G. ;0;.n• _di• r, art0ht•_ USF~College of ued7Cine, U nospltal, Tamoa, * FL 33612. A sing)e /ntratracneal dose of either ben:o(a)pyrene and FesOs (IP+FO),(S s+g each or I eog tach), or FetO, (F0). (S tg or 1 s9), suslxnded in saline ws given intratracneally (i.t.) to Syrian golden hamsters. Animals were sacrificed at l, 2, 3. 4. or I rks after treatment. One nr before sacrifice they were given 300 uCi 'M-thrntdene t,p, DNA synthesis ws ases- sed by qtNntitative autoradiograbhy. When CGnpared to Shae- treated amr ls (air i.t.) namsters given 6P • F0 or F0 alone sftawd an increase in labeling tndes. Ciliated cells were not labeled. Reduced wan grains/cell were seen for a period of at least a vlts after treatment /n animals given !P • F0 as t:o.pared to those given F0 alone or shao treated. Although a•Sttonly found in cOntrols, very heavily labeled cells rere rarely observed in !P-treated an!wals. In anotner series ef esotriatnts, normal hamster tratheas vere incubated in shon-ters organ culture (J. Natl. Cancer Inst. 49:743, 1972) for 1, 3, or S hrs with SO uCi/wtl tM-thy+sidtne and 0.5 uM or 2 tJ1 N-aethyl-N-nitrosourea (NMU). Autoradicgraohy revealed that sa7re than 90% of all types of epithelial cells were labeled, lncreasing with increased tinoe or dose of !afU. These results suggest that the DMA damaged by respiratory carcin- ogens +n vitro can be repaired. The fidelity of thts repair ts unktqrffl=Supported in part by NIN FS-73-206). 2206 pafiOlear HISTOPATHOLOGY OF RESPIRATORY SYSTEM OF 810 15.16 HAMSTERS CHRONICALLY EXPOSED TO C.GARETTE SMOICE. F. Homburter, P. Bernfeld and A. 3. Russfield. Bio-Research Consultants, Camortdge, Mass. J21i1 Secauae of its resistance to nicetine toxicity and as suscep- tibility to caretaogens given by various routes and to smoke- iaduced respiratory lestons, BIO 15,16 inbred Syrian hamsters are especially suitable for chronic smoke inhalation studies. Data are reported on 101 hamsters exposed co smoke from 1Rl Kentucky reference cigarettes in a lValton-Morrusey re- verse smoker iAEC Sympostum 18, 19701 for up to ?0 .veeks, and are compared with observations on 60 sham- smoked and 60 cage-held hamsters. Sixty-eight to Tir, of the control lar- ynaes were normal, whereas onav 4^, o( the smoke-exeosed Larynau bad no pathological lestons. The smoite-esposed lar- ynxes showed microinvaslve carcinoma - 19•., additional pseudoeptthelwmatous hvperoiasia • 21 :, leukoplakia - 4Or., and squamous papiliomas - 15r. The few leaons seen in con- trol animals Wore chronic inilammation. c3r, of the smoae- e:oosed an,mals and from J:o o'', o( the controls sho.ced changes of puunonarv alveolar macropha;es. i', of :he smoke- ecposed antmals had tracneal sou_mous paorllomas. :-o anr- mals arnor•g those exposed to smoKe had nasopharynpeal tu- mors. There was a st;nrfrcantlv increased rnctdence of focal myocardial de;eneratton in smoke-exoosed B(O 15.16 hamsters. 2207 0arw1,0Gr PCIITOREAI `ItSQTNELIO>u. ytlton Kannerste:n• and Jacob Churt )Sount Sinai School of '<edlclne, vev Yora, ::.Y. 1:C29 and Y nert loesercal Mosptcal Center. Pacerton, s.J. 07S1t Elghty-tvo cases dlaRnosed as aallenanc perltoneal aeso- thelloea wte studied. Ftftv-six patients (6!:) vere known to have had asbestos expesurt either br histor• and/or the !•resence of asbestos bodies in lunt sections. Seventy-four perceet were between SO and 69 vears of aee. Only S were te- Yles. The cases were traded accordlnt to certainty of d Lr{- aasis as deflnlte (2cS), probab)e (4t:) or possible (26.). This ves based ee clinical inforsutlon and gro u and atcre- sce0ec serpholoty, ietludlne autopsv findtnes (available le )7 cases). T1e serpnolotec criteria v.rr: alftuse, or sp- parestly spreadtnt. tnvolvesent in the pertconeal cavttr, absence of desonstrable estts-cotloae ort.arv slte, and a cytolotle character describable as eplthelial (75: of cases). .eseechvisal (<:) or alaed (:1')• The eslchtl(sl and slaed types fer.ed distinctive patterns in ): ca.es. In 22 cases the hlstolotr Ms noMtpeciftt, saee shovlne Yr\ed alYplaata asd plee.orpnisa. The deeonstratton e! M•alurontdaae-s.nst- tive acid eucosubstance, dlsrnosttca117 strnlflcent in ept- tttellal or alaed tuwra, was nostetvr in 14 o! :3 cases. No earrelatlen was established between cell tvpe er pattern and bloloitlc behavior. Survtval ttat after onset of sveetoes was brtef, in eosc cases free e months to I vear. However there were aaveral instances of vell-dtffereetttteJ .esotnelloea which were slow Rrevtnt. iSupperted by USIHS Crant 0.ti-0091l1 1,.,X )m ) 1 l)N 0--`7 25d.-' i,.,!