Tobacco Documents Online

HT- 135'6. From the dSk oc w. T. HOYT I TeLruz.ry ?l, 1S66 At the weet:rsE oa Tebrvery 1, 1963 vitb Dr. iyacb, the requt:t vas asde for c rc=or:ad= caveriase sv;,gested are;: a• reserrch. Accord:rbl,y, the :tt;,c9ed ae=:raa3u: Las Deea preprrsd by Dors. Littie aad Roekett. 1'.S.li. (Above also to Nessrs. Yeamaa, Grant, Smith & Ramm.) T!+ C...d ).r :.i..w s.a..eh • oi.A. W 71Vd A.sr. x.. T..k. 1/. T. 10071 i pLA1NTIFF'S HIBIT 20 .u 4r .n ua.. r..w. oo THIS DOCUMENT SUBJECT TO CONFIOENTIALITY AGREEMENT. 204 , CTR HN 026439

'I-FiE C-UI'.VC1L FOR TOB.aCCO RESEARCR-L:.S.A. PROPflSED FIELDS OF RESEARCH The following memorand= is divided into two genercl sections: (1) A brief discussion of the present situation, cnd (2) An outline of main plans for future vork. 1 THIS DOCUMENT SUBJECT TO CONfIDEliTIAUTY AGREEMENT. Z( o CTR HN 028440

. . 1351(~l Present Situation The present situation is a product of: l. Continued development of the grant-in-aid program which is proceeding satisfactorily vith a steady interest in research as sho+rn by the nucber and quality of the applicants. Methods of selection continue to be rapidly enforced, and every effort is made to obtain all the necessary information on each grant before a decision on it is reached. 2. I Development and implementation of the list of researcb projects discussed over a long period with the Ad Hoc Coffiittee and vitb Genera! Counsel. The topics included in this list are in the correspondence and reports which have been excbanged by the staff and.tbe representatives of the industry. In order to save time aad duplication, they vill not be repeated in this memoraadum. Certain general administrative procedures apply to both the grant-in- aid and project programs. These include pilot studies initiated by and made for the staff; informal conterences with groups of different sizes to bring the staff and Scientific Advisory Doard up to date with fields of scientific research of direct interest and importance to then; liaison with the research group of the American Medical Association to avoid unnecessary duplication and to exchange azy vievs or information vbicb may benefit either or both programs; continuation of the Fellovship Program for qualified medical and dental students vho are planning to enter the field of research in these disciplines and vhose interests may affect the ultimate progress of The Council's broad research program. Flans for Tuture Work It should be kept in mind that we are studying a field which involves life processes. 0ur rnovledae of these changes rapidly because of relatively Dev techniques available to research workers. The statf atust, therefore, be continually alert to the existence of such changes and should take the necessary steps to utilize them either by inclusion in its existing program or by evaluation of thea as a possibility for future suidance. Reviev of the program may, for convenience, be divided into the follovin,d general lines of work. A. Development of Mtltivariate Analysis This field of staff activity utilizes at the present time the services of Iurt Snslein, who is a specialist in development and nse of computer methods in this type of analysis. !(r. Lnalein vill be used in a number of capacities. These include advice on the planned and existing work of Srantees to insurs as far as possible the best opportunities for analysis of vhatever statistical aaterial that may obtain in the course of tb ir experimental or clinical work. Also included is a searcb for e:perimcntal population.s vhich can be advantageously used in this type of analysis. This vill involve siti THIS DOCUMENT SUB)ECT TO CONFIDENTIAUTY AGREEMENT. ZI ( , CTR HN 028441

2 i I :. 135 :~ visits and conferences in many cases. A third type of service is thet cf a general consultant in helping the staff to evaluate opportunities for extension or development of new fields of more sophisticated epidesiolo5ictl study. Finally, the staff is interested in Mr. Enslein's own resea.-ch on the improvement aad extension of inethods of analysis of a mathematical nature for vhicb his training and experience fits him. B. Inhalation Studies 1/ost of tbese are naturally included under the beading of animal experimentation for this field of laboratory work vill continue to be of broad interest and Importance, aad it is necessary to do everything in our power to insure proper monitoring of animal material and the control of variables. The latter problem includes genetic control of anime.l material, standardization of methods of applying vbole smoke and of preparing tissues for microscopic study aad pathological interpretation. To some extent buman material may be used in sucb fields of resea:ch as more accurate measurement of degrees of inhalation. In sucb studies, comparison of various species of animals including non-buman primates may vell be involred. Througbout all of this work, two general points of view apply. One of tbese is the contrast of vhole smoke versus artificially-prepared condensates. The second is the reaction of skin versus lung tissue. In both of these points of view, possible effects of any solvents used vill be considered. C. Oral Cavity Research The progrsa of this type of researcb started by the staff vitb the approval of the Scientific ldvisory Board is developing satisfactorily and is giving interesting results. Various factors sucb as accessibility, dietary effects and clearanca x cbanisms are important subjects of researeh in vhicb the oral cavity bas distinct jdvantages. D. Tissue Culture The rapid development in the field of organ culture has added a new dimension to that of single-cell layers, vhicb vas the earlier method of studying tissue reactions in vitro. The staff is interested in contact vith researeh workers in both phases~o tissue culture, and baa plans for a conference on this subject in the nr ar future. It abould be remembered that The Council has really been responsible for critical and valuable support of tissue culture vork alatost since it vas organized. It is clear that this has been a fruitful line of endeavor and tbat it sbould be continued in the future. _. Virus Participation in Cellular Changes The rapidly iacreasing interest in lat4nt viruses as a possible contributory factor or cause of neoylastic disease is clearly recognized througbout the scientific comanmitp. inere is good reason to believe that the lungs of organissu as vtll as otber tissues in organs vbers influences of tobacco have been elaisied by soma are possible sites for the activity of sucb viruses. Studies of tbis tTpe arm focused primarily on animal work at THIS DOCUMENT SUBJECT TO CONFIDENTIALITY AGREEIZfTZ CE9 R E E i"i t.,i 2-8442

f present, but there is reason to believe that such vork vi}ll~lead to ~ a 6 4 have a direct bearing on vork vith bumans. The staff is active in developing this approach. F. Bivaleat Lffects ' of Nicotine These have now been shown, and indicate that effects of a beneficial nature aay be involved. Ruther studies of the effects of nicotine on the central nervous system vill be important but should be, in a11 probability, largely confined to human material and sbould be of an exploratory nature rather than being conducted by The Council in depth. M example of this sort of opportunity . is suggested by the vork of Darbara Bro+rn, vhicb involves the study of different types of brain vaves in relation to the use of tobacco. This vork also provides an example of bow it suy be possible to use ltr. Enslein to discuss the best methods of multivariant analysis in recorded brain vaves of different types and frequencies. In general, two points of view apply to all the vflrk of Tbe Couacil. These are: 1. The cosrplexity of origin of all the diseases statistically associated vith tobacco use, and the inadequacy of the single factor method of approach to this vhole problem. 2. Tbe basic isrportance of the so-called 8ost Faetor. This means the innate or genetic differences betveen human iAdividuals vhich continue to develop aad operate tbrougb their lifetime and vbicb results in distinct and individual differences in susceptibility or uagusceptibility to the various enYitonmenta.l cballenges vhicb their life experience presents. One may safely say that vhile such boat factors may not be simple or easy of analysis, their presence and function in any adequate analysis and explanation of the causation of the various diseases involved cannot be ignored. The problem therefore is to keep tbase tro general factors -- coarplexity of the diseases and importance of the Bost Factor •- continually in mind, aad to conduct the various specific pieces of research v'hich ve svpport in such a vay as to add to the increasing body of experimental evidence vhich justifies this attitude. THIS DOCUMENT SUBJECT TO CONFIDENTIAUTY fGREEMENT. 1 2j 3 CTR ["IN 026443