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Memorandum to Dr. J. D. Mold Page -2- July 28, 1977 guaranteed those results in advance. In emphasis on current progress on this project, one should not lose sight of the fact that it is in very large part dependent on a far sighted canage;-ent that has supported continuous research in this field for more tha,n tventy yeazs, at varying levels of manpower. Much has been written about the decision of the British government to allow the marketing of cigarettes containing about 25 percent of either of two synthctic tobacr os in the blend for the purpose of reducing the biological activity of sroke. Such level of replacea+ent of natural tobacco with synthetic products results in or.:• a marginal reduction in biological activity. In this case as well, biological activity refers to skin papillomas produced on painting smoke on the back of a mouse. Adauttedly, higher level of reduction in biological activity of smoke cay be realizable with synthetic tobaccos at higher levels of substitution. In no case how ver, ha.s the British tobacco industry demonstrated reduction in biological activity of the order achieved through ouz development. RESEAFtCH CENTER Dr. A. G. lCallianos hGK:dfh COPY 1.r ie T`+ i i t"i 026306

14qrri S 14. l.lggett & Myers Tobacco Company Memorandum Date: July 28, 1977 To: Dr. J. D. Mold Fro m: A. G. Kallianos Subject: Statement on what We Think that We have Accomplished on Project XA-SCC: The.following is a suaamry statement of our accomplishments in reducing t.`.e biological activity of cigarette smoke. Briefly, as a result of 20 years effort in cooperation with ADL, we have deverloQed a cigarette system which produces sa+oke of reduced biological activity. The activity referred to in this context relates pri®arily to tumorigenicity of smoke on the skin of the mouse. The animal test procedures used conform to accepted practices by investigators across t.he world in a number of research laboratories and institutes and are in accordance with protocols established by the National Cancer Institute. These entail collection of ssooke by cold-trap condensation and applica- tion of whole smoke condensate on the back of the mouse at 50 mg per application, five days per veek for 80 weeks. In mouse tests of this kind, it is customary to count as papillomas, or "tti:nors" certain types of changes in skin app.arance, whether malignant or not, on the grounds that these measure biological activity. This year, for the second time, we have seen one of our treated cigarettes give 80% fewer animals with tumors than the control tested alongside. The number of animals developing carcinomas is also dramatically reduced, as cosspared to the control, although these numbers are less satisfactory as a criterion because they are so small. Our experimental cigarette consists of a blend of cosm+ercial tobaccos treated with small amounts of two additives. One of these is a normal cocponent of tobacco, especially burley tobacco, and the other is used extensively to purify automobile exhaust. Our results indicat.o that when these two additives are used in combination the overall effect is greater tlun can be achieved with either one independently. Research has b..a in progress toward developmant of an optimal filter cigarette involving the saae additives. None of our filter version treated cigarettes have been tested oa the 80-veek mouse test. In fact, w would not expect 80-wek mouse tests to show an additional effect for filter, because those tests do not depend on milligzasu smoke yield per cigarette. However, evidence has b.en generated in our laborat.ory showing that our filters can reduce levels of some health-related smoke components oth.r than those prinarily associated with tusroriganicity. T'he nechaniss leading to reduction in biological activity is not well under- stood. The additives w re deliberately chosen, and on the basis of tests we can do short of animal tests gave results that w re regarded as highly promising. We were not surprised at the favorable outcocrs on long term, but we could not have COPY ..r 1 ['r+ 1 = ~ "4 ~.+~ . ~ ..~ 4~ ...~