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PRIVILEGED AND CONFIDENTUL iiggett & Myers Tobacco Company • Memorandum Date: July 13, 1977 To: J. B. Ross From: J. D. Mold Subj.ct: _ Proposal to Include a Skin Painting Test at ADL to Evaluate the Level of Tumor Promoters in Smoke from Cigarettes Treated with the Catalyst Mixture At our last meeting in Boston with Dr. xensler, it was suggested that we should include a skin painting test to determino what,Rer we have affected a reduction in the tumor promoting activity of cigarette smoke by incorporating palladit.m catalyst and nitrate into the tobacco blend. Previous tests by A. D. Little and others have demonstrated that smoke from normal cigarettes exhibits in addition to a relatively weak tumor initiating activity, rather strong tL.nor pzomoting action. In view of the effectiveness of the palladiun nitrate additive in decreasing the total tumorigenicity of the smoke and its relatively lesser effect in lowering the PCAH content (believed responsible for tumor initiating activity) it seems reasonable to assume that the additives may also-be effecting a decrease in promoter activity, as well. If this could be demonstrated, it would be of some additional value in explainin~ the na:;:re of the catalytic effect and might also add to tre positive attributes of our product. To conduct a test for the purpose of demonstrating pronote: activity would require five groups of mice and approximately 12-15 ronths for completion. Treatment by skin painting would ba-as follows: Grou one initiating dose, 3 x 100 ug D(a)P weekly paint:ng with 3 x 13 mg condensate Cigarette ty?e 1 Yes No None 2 Yes Yes Contiol 3 Yes Yes Catalyst,c:g. 4 No Yes Control 5 No Yes Catalyst ctg. 2 ~L.AMfT~f ~ ~Sn1ri S1 /..7~ S i M F.C_ 61*~14101 ~ COPY ~ CTR !tN 0G'6303

J. s. Ross July 13, 1977 Page 2 The results from this test would not be available until about Sept.-Nov. of 1978 and could not, therefore, be used in the Spring 1978 introduction. It might, however, be useful as the basis for a second wave of publicity. In order to carry out the tests described abov' without requiring additional funds it is proposed that we eliminate one of the two production runs of the low nitrate sample (with uniform distribution of catalyst and nitrate) from the tests due to start August 1, 1977. We would still be testing this cigarette modification with one production run and 100 mice. It is proposed that we use only 50-mouse groups for the promoter test. Since the B(a)P initiator test group (tl) reSuires no smoke preparation or painting, this would be relatively inexpensive. The use of low doses of smoke, 3 x per week for the other test groups and a shorter duration, would actually lighten the smoking load over that presently planned and may result in a small cost saving. The principal cost increment would be histopathology on 250 vs. 100 animals. This is estimated to add approx:mately $1000-52000. However, this charge would not occur until 1978. ~ ! /jf cc: J. H. Greer (2) 9 r- 60 141(:,: COPY GO% CTR MN 026304