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TAIt1.E (/F /:Q1NTF:N'I'S PaRe Introduclion . . . . . . . . . . . . . . . . . . 5 Research FindinRs and Studie~ . . . . . . . . . . . . . /t Abstracls uf Reports Sludics at the Cellilar l.evel . . . . . . . . . . Itt Pulmonary Physiolosy and Anatomy . . . . . . . . . 22 Cardiovascular Studies . . . . . . . . . . . . . 23 Psycho physiokgical Studies . . . . . . . . . . . 11 Tobacco Chemistry and Diiuhemislry . . . . . . . . ]b Pharmacology . . . . . . . . . . . . . . . 411 Other SI udies . . . . . . . . . . . . . . . . 41 Review . . . . . . . . . . . . . . . . . . 41 Recipients Of (7rants . . . . . . . . . . . . . . . . 44 m A B

S/:IF:NTIFij(: AnvilSOttY I?U1ARi1/ to the Tobacco Industry Research ('ommittee KfiNNl31 I I MI?RRIf.L LYN('FI, M D., Sc• I) , LL I) ,('hair,nan ( hiwrrR,.r rrnd 1'rn/rr.vrr nl 1'athrdoRy McJlcal ('c,11cPe of South Carolina ( harlcdnn, South Carolina RI(•IIARh ) I1ING, M D. /'n,frctor and ('hairmon. Department of Medicine Wayne State I Iniversity Colkse of Medicine 1)ctrnil, Michigan McK f:FN ('Al-1 FiI.L, Pu F) , M f). !'r(-frtuir F.mrrituv r,( f'harmat-nGrty ('iwncll I /nivcrsiry Medical ('n11cRe New Yrnk, N Y. l.f(1N () lA(•OHSON, M I) 1'r Irrc N nnd ('hairman Department of Medicine ltniversily cd ('hicasn ttirr( n,r, ArRc+nne Cancer Rtesearch ftospilal ( hicaRlr, Illinois PAIII. KOl IN. M 1). ('arcinnRcnesis Studies 1lranch N:rliunal ('ancer Institute FSethcuQa. Maryland ('I.ARf:N('U. ('(N)K l.fi'11.E, Sc 1) , 1 1. 1) , 1.trr I). Sf irnrifir fl~rrrtur, lnhaccu Industry Rcacarch ('rnnmiltre Ihrrrr,r f- rrrrrifut. Ruscoc 11 lacksnn Memprial LalN,ratnry Har Ilartw,r, Maine STANI.FiY P. RFIMANN. M D., Sc•.f). 1)irrct,w Emrrnur, l he Institute for Cancer Research Philadelphia, Pennsylvania WII.I.IAM F. RIFNIIOFF. 1a., M.f). 1'rn/rttrrr Emrritut Ir/ SurRtry Jnhns flnpkins Universily Schoxil of Medicine Itahimore, Maryland EDWIN I) WILSON, Pn 1) . LL I) 1'rnfrru,r Fmrritut r,/ Viral.Sfnriclic.t Ilarvanl Ilniversity ('amhriJge, Massachusetts f(ctltt {( I(' IRx'KI I"1, I'rr t) 1 AI(1RR1CON IIRAIIY, M 1) .1 "n'Lrfr 1 h,r,h,r 4 lli,, I,Ifr .Cf Irnrl/'ir f lfrn ron Introd«ctiori During the past year the Tobacco Industry Research Committec has continued and extended its suppcxt of research. 'f he major direction con- tinues to f.e intn sonre of the many clinical and eaperimcntal factcas that ttill need to be itknlificd, investiRated and evaluatcd in ccrnsideratiun of the origin of lung cancer, cardiuvascular diseases, and othcr cuml.lex constitutiunal ilments. While these research atudies have increased our factual km+wlcJRe, they have at the same time continued to makc clear and tu eml,has'vc the great and critical gaps in that knnwkdse. They have cun/irnrcd tlre sr,und ness of the position hcld and expressed by the T.1 R('. This position is that there dues nol exist tlre esscntial e.f.crimental and clinical knowkdse with which science can even dclinc or idcrntify thc multiple factors or influences that may contribute to thc origin and lrrrrRress of these diseases. This statcmcnt hokls good ls a Reneraliraticm. II also apPlies with even greater siFnificance to jud6mentl concerning the causes cd Ihesc diseaus in any individual human being. 1 here have hecn and will cc,nlimrc to be speculations and opinions on the causes, Dur it it a rrfultrr uf .uirrrti/Ir fact rhar, in our prr.rrnt ttatr of knuNdrdgr, nu nnr An„ws the „ncwrrc R.prrirrrv .Srnd1.. Inconclusive Reported epidenriological findinRs, resultinR (n,m siuril:rr turvcys that use yucslinnnaires and inlerviews, indicate certain ":rcaKi:rti~~nc" tx•Iwccn the incidence of Ihcse dixases antl one or mure cnvironmcnt.rl /actort, includinR tc,hacco uce, especially when that p:rrliculor faclur ic .inyh-J ~,ut fcx study. The mcthoMhdopy crf such auvcys :urd uL,r Iht intr//~rcl,ru~~n nf Ihcir reported findinFs have been caimincd and t'rilit'ircd hy t t -nypctcnl ~leus ticians artd cPidrrninlnRists 5

It is not turprisinR that cvery timc anothcr -mnvcy of this typc ia crm- ducted unJcr cnnditums that are hnridly simil:rr, the aame Rcncr:rl rcwlts are ohtaincd f luwcvcr, the rerrrrnnn of :rn inJircct or uns:rlictactrrry ~cicn- tific pnccdure does not add to the reliability of the data rrllcctcd by previnus enorts of the same type. Reviews and reports cnntinue to appear, bascd mrxtly on sl:rtictically- orientcd survcys llre unquestioning, unreserved enrlrrrscmcnt, as nrncluave, of each such rerHitive report, which contains no ncw ur original d:rta hut amnunts to a statement of opinion, is a disservice lu Irue scientilic rcsearch. l his places an obstacle in the path of accurate recnRnitinn and analysis of the problems that remain before tts. As stated, great gaps exist In knowkdge of the causes of cancer and of cardinvascular diseases. These result in a IarRe degree from Ihe lack of adequate nd coMrolkd nxthoKls of bioa><say of suspected processes cx suhstances, and from the absence of contrrdkd clinical studies fully utitirinR availahle biochemical tests of metabolic and hormonal changes and tkvehrp- men,s under diflerent physicat, physiokrRical, and mcnlal environments, /mproo.m.nt oJ R..enrch SouRAr The T I R('. is continuing its activities in supporting scientific studies nd in c<mductinR confcrences of scientists in order to rklineate mcthrals (d imptovinR research in these directions. As ideas and opportunities for the est:rhlkhrncnl (if ncw rcacirch undertakings are defined, studies will he initiated and crmtinurd for wlrrl- ever pericd Js nccessary to scdve the rnany complex prrrblrrnc that uni- versally are recognized as lying ahead. It is hoped that it may sr>trn f.c (+o•.ihle for var:<rus intcredcd orpnni- zations, now conducting or supfwrrting research indcfiendcnlly crf onc arr other, to coopcrate in organizing prnFrams of mrrre srrphi.tic:rted and potentially more significant studies aimed at filling the gaps in our knowl- edge. This should kad to proRress more intelligently than doxc explrriting exclusively any one over-simplificd hyfx,thesis of specific environmental causation, or of sekctive emphasis nn any one factor statistically asauialcJ with certain causes of death. !Yn Slmpl. "MnRIc RulfrP" E:l.t. The need for a broader, rnure milurc and mme diversifirrl I1rofram of research was evident in results of some invcstiR:rtinns rrf+ratcd durinK 1962. Uuring thal year, scientific work contimrcd trr auplxrrl the view that the quest for a sinRle, simple "magic bullet" to explain the causes or pm- vide the cure for cancer and of heart diseases is unrealistic and probably futile lhe interaction of many constitutional and envirrmrnental factras and influences, some of which still may be undetecled, is becoming incicas- ingly evident. For this reason alone--the fact that research indicates a possible in- volvement for these many factors and inffuences-it is at present scientific- ally unwise and indeed may he harmful to attribute a simple, definitive, causative role to any one of them, or to attempt to assign them relative degrees of imporlance. The smoking of tobacco continues to be one of the subjects requiring study in the lung cancer probkm, as do many other agents and influences in modern living Science does not yet know enough abtwt any auapected factors to judge whether they may operate aione, whether they may of.erate in conjunction with others, or whether they may affect or tre affected by factors of whose existence science is not yet aware. Indeed, it is not known whether these factors actually are "causative" in any real sense. "' 6 7 r~

Ile scarcli I~ indings arid Studies flurinR 1962 the Scientific Advitory Board to T I R C made Rrants for a number or new prnjectt and awarded grant renewals for c(rntinuinR wnrk in the broad areas of laboratory experimentation, clinical invcttiRa- tions, and epidemi(.h.Ry Progress of the research is partly reflcc:cd in pal.crt puhlished by recipients of grants. Since the 1961 Rcpnrt, hll addi- tional repats have been published, bringing the tntal to date t(r 2112. Abstracts of the new papers appear elsewhere in this Report, and may be consulted for delails on pr(>Rress of research. All Rrants awarded by the Board are for studies that prornise Irr ad- vance the Board's objective of stimulating research that will prrwluce help- ful knowledge atarut what is Involved in the problems of lung cancer, coronary discau, and other ailntcnls. Viruaes and Cancer World wide scientific inlerest.in Ihe viral theory of carcinr•Rcnetit is obvious and powirtR Considerable attention was given to this subject at the Vlllth Internatitmal ('ancer ('t)nRrctt held the summer of 1962. Some 50 papers relatcd to Ihe patihihty r+f a viral etiology of cancer were presentcd, cnmparcd with only four p:q+crs on that topic at Ihc ('ancer ('onRrrst held only fnur yeart prcvurutly 'lhit inrrcattd intcrctt in and attrntion trr virus cancer rctcarch it indicative or Ihc r:rl.irl chanRct that can take place in retcarch ctmcrrtt irrnccrninR canccr. Vinrtes have been thuwn to in(luce a varicty of canccrt in several nimal sprcics In 1962 there were rcpatt Ih;rt human adcnrrvnutes known to cause resriral(xy ailmentt snih as coldt, when injeclcd into c.{.cri- ntental animalt, had resuited in Ihc dcvel(lI+mcnt of ('anccrt in thc animalt As with other aRcncics, the *1 1 R('s rctcarch Rrants in Ihis field have been increasing in recent years One T:1 R(' - supprrlcd study is seckinR lu dctcrnrine whcncer injected vital aRentt, either alone ur in cornhinatinn with variout environ- mental factors, will result in meatrnahle clfc(is on the IrachcnF,r(mchial tree and pulrnnnary littuct of lahoaatrrry aninrait 'Ihit wrrtk :rltn will Ix cxtcnrlcd In intlndr titurrt in nryan cullure nnnthrr tludy in prnRrett invnlvet Ihc qurttion of any rnlcrrrlnlrrl rulr thyt inflrrrnra virus rnteetnrnt, r.hnure tu ( IR.llrttr trn.rke nnd r.lhrr /er tort may havr in Ihc rlrvrlnhmcnt nf fwlmnnary anrl hronrdrial Icv.rnt in rnr((• Ihia (•.urrcul.rr .IU.ly it an retrntii.n r.f work that Ir.r% Lrrn tul.I•.arrJ Inr .rvn.rl yr.u~ rnru rlv r.urrl.orrl hiN.,I~,F•y r ylr.l,.ry, uml r yrr.Y hr nrr.lry or thr u.r. h rJ.r.m hr.J Irrr .urrl Irmrt r,l r~l. nnrcnt.rl mmnr.Jt I Also under way is  project involving the use of chemical agents in virus-free animals to see whether it is possible to clucidate Ihc rclahunship, if any, between viral and chemical carcinogenesis Cell Studies Of vital imptrtance to virtually all esperimental scientific endeavnr is work involving the cell. For years the T.I.R C. has been supporting hasic cell research, aiming primarily at the training of more experienced workers and at the development of more reliable and accurate techniyues. While previous interest lended to concentrate upon the morph(doRy of cells and morpholrrical changes, the emphasis has now been shifted In the area of the metaholitm and behavior of «lls in resl+onse to varirnn Ireatmentt Studies now in progress aim al describing basic dillerences between normal and ahnormal, or cancer, cells in virro, rcRardkss of how the cells may be affected by the challenge of some external asent. Of course. In a sense a normal cell in a culture medium is not exactly "normal" because It has been removed from the medium of its natural host. In (wx laboratory scientists found that continuous ultraviolct irradia- lion of the nucleus of a living cell produces damage to the nucleus Smrilar damage was found to occur with the use of certa•Kr chemical aRents. One imporsant implication of this particular work is the possibility or developing and prrfectin6 a more rapid and accurate bi(iassay methad. A series of agents, among them polycyclic hydrocarbons, are now being tested in the hope of developing a semi-quantitative biuassay for relative carcinogenic Ixrlenlial or activity of a multitude of suhstances. On April 21, 1962. the Scientific Advisory floard sponsored a con- ference on cell differentiation at the (nstitute for ('ancer Research, Phila- delphia. Among the topics discussed at the jnfnrmal session were the lac- tors within cells kadin6 to and guiding diRerentiatian, and the inlluences on cells of various environmental factors, ('hemical recrmstitutiun of un- differentiated tn differentiated cells received Altentiun l)itcuttcJ were crllu lar and tissue p+lentialt, influence of chromosomes and Rencs, varirrut frrnns of "mutalion," urprniration of tissues, and other topics. In addition to sta0 members and memtxrs of Ihe SAIf, the /ullrrwinR were present: 11R /()/1N 1' HONNI R. /'rIn(rlon U.dvrr.ity, 1'rin(cton, N I I/R IN)N W FAW('Y.1•1, It.rv.rJ Univrr.ily, ('amhrldRr, M.,t t)R ('l 11-1//R1/ W GtIRNFV, ttnrvrr.lty o/ ('hic.g.r, ('hr,.p,, tlt 1)R At 1 N117 1' MIRCKY, Rr-Ir/rtlrr In.fitutr. New V.vll t)R /(//tN NtINNCIRt)M, Wrunrr (irrn Intrllulr, \t.h~huhn, S..rJrn 1)R. F. (' SIt Wi1R1), ('urnrll Ilnivcrsity, Irh.ca. N Y 9 x

I rnrn Ihc InttUtnte f++r (•anccr (tctcarth A(ARI! Ila 81 RARI/1N(1• I'h I) 11 R()t•(t-, I t R/: FI), Ph 1) l 11()MAS 1 KING. 1 h 1). A I IMA 1)F UAINF, Fil 1)ir , Rctcarch I cllnw MARIIN 1. NI'MhR, Phl). RUI)I:R T P. PF RRY, Ph 1). (:I'ORUI? RUI)KIN, Ph 1). lA('K S('HUf.TZ. Ph f) l IMOf IIY R. TAI PUT, )R , fN f). ('ar(liot+a+(•trlnr 41'erdi(•s Surl.r.rt of cardiovascular retearch conlinucs U+ he an imf+++rt:urt f+art of Ihe Advitury Ilr+ard's recearch prr+gram. T hit it a cuntl+licatcd and carn- pfet fic•hl and cncrrmpatses, of neccccity, a variety of sfa•cific tr+piu:t Work cr+n/inucs tm a T I R(' cupp+rlcd pn+iccl which it ceckin6 to dcterminc if cnvirrmmental factrul have any effect on the dcvctnpmcnt r+l athcrr+sclcn+tit at revealed by autofny atudict uf accidcnt viclirnt traincd interviewers are suckinR to Icirn all they can :rhr+ut Ihc victim's life -hit dicl, hit smoking and t+thcr hahitt ti+ c+nnl+.uc ++r cnnnccl thic inh+rma- liun if I+rntihle wrlh the aut .lay +I it + In Ihe tarnc Rcncril fu Id .+ I I k t t r+nir r h r, it 1-rrr• 1 lindmr Ih:rl durinR tmc+king thcre w:)c a r+.c in •- I.ouN .++r,/. (I I A ) m 17 palicntt with hcalrrl myr+cir+lr.+l rnl.+r+r-n 1r, h.l A Icv.rr n•c w:rc found durinR smr+kinR in I(6 n++n +„r++n:+rv I+.rncpt., :nn1 thc tc.c:+rther suRfesaccl that the Frcatcr FTA rctP+~ncc in rlre cunm:rry P:Nic•nlc rccaltcd fmnr a Rrcatcr rclcicc of catechr+limine after nicutinc slimul.rtir+n I F+it chf- ference in reap+nte, if ct+nfirmcd, may reflect a cunuitulir+nal Jillcrcncc hc- twcen furumt who are prcdiya+ccd lu cc)rcmary diaatc and thu.c whu arc md It it, (+f cr+urtc, nul known what Ihc cRcctt may t.c• rd rerx-titivc ricct in the f.IrHKI Icvclt r,f FFA, whic h actu:rlly are a vital fHtily fuct I'mvt murtcm cr+mparicnnt nf Ihc degree nl athcr~+cclcrntis in the curnn:rry aricrics r+f tnu+kcrc and nr+nvrtukcrs have thuwn no tiFnilic:+nt dillcrcncct Anirn:+l cxf.r•rimcntt tarried rwt under Ihe I I R ( ' f+n+pram have :+Icn thr+wn nr+ clcar rtilTcrcncct in Ihe dcRrce d alth+ r++cclcn+cic in chulcttcr+d Icd animalt ++f tevcr.J tf+ccict at a cnnscyucncc +d chronic nitutinc irrit:rtnm SnrnLlrnR an+f Itt.rnrl Flow Of iinf-rt.ince in the c:+rJir+vaccul:+r ficl+l ic a rcccarch f+n+jcct that a nl.l t tn in+ aij•,'tr Ih(• cllt +t I+f r„I~.++tn tnwkinP rm tkclct:+l muctlc i blood fluw. I hcrc has been conflicting evidence on Ihit suhject in Ihc few ftudiet exittinR in the mcdical Iileralure. Working undcr  T.f R.C. Rranl, two Ilc+ston investiRah+rs uced the disappearance rate of a radioactive iscNope fronm skeletal nroscle as a measure of its nutritive blood flow. (N 17 normal subjecls studicd. 14 showed an increasc in skektal muuk nutritive blood flow as measured by the radioiccllupe disappearance rate during cigarette tmnkinR dccf,ite srnall changes in total calf blood flow and the usual decreases in skin tempera- ture. 1 he mechanitm of such an increase slill remains unknown According Io the scienlitls, lhis wc)rk reveals constancy of the increase and suRResls hat inhalation is not a factor. The investigators reported that evidently an inlact nervous tupply to Ihe limb is unnecessary since three of four sympatheclnmired limhs also +howed an increased disappearance rale during smoking AttuminR that skin blood flow docs not decrease in sympalhectomircd limht during smok- inR. Ihey said, the increased disappearance rate does not represent a simPlc shifl of hkKxl from the vasc)constricted skin to the musck. Nlrotlne ond Cor6on A/onorJde The Scientific Advisory Roard has alNl aulhorired a study to sce whether determination of inelaholites of nicotine or of carbon mum+otide in the blood s(ream will Rive more accurate infrrrmalir.n nn the ealenl to which cigarette users inhale the smoke. Nicotine is ahsorbed in some dc- Rree in the buccal cavity and lo a greater degree folNlwinR dccp inhalalion uf unoke. Carbon monoaitk, on the other hand, is reputcd Iu be absurfxd only on deep inhalation. Perfection of such ntNhods may permit mnre accurate dclermination of true physir+luRkal smoke eRpt+ture front cigar- eltes, pipes and cigars than yucstinnnaires on .mrlkinR hahi/s and Ihe depth of inhalation, and thus may raise correlation sludicc to a new level of reliability. T'he methrxlt dcveh+pcd in this ttudy will be utcd by the invettiRatras lu help intcrt.'rct resulls of anulher project on Ihe relatir+nchip hclwccn steroid levels in hrxly ftuids and various forms nf hr.ul ditcate aml crrl:+in lyres of maliRnant tumors. Thit latter prr+ject is tu he tulqw+rred by a federal agency. An invcstip,atinn hat hccn inaufuratrd with rcFarJ tr+ c.uh+.n rni+n r+tidc The amr+untt r+f carM+m mtim+aiJe that can f.c :rhsr+ncerl cv+•n rtur.nl-h very heavy smr+kino have been shr+wn Ir+ lie well f.ch+w Ihc tr+tic Icvcl S+nrc the Iracct uf carbon munotidc Prcccnt in ciy-arcUC smr+kc arc rcl.+uvcly cnnslanl. Ihit ttutly will seek lu dctcrniinc whclFher hI(NMI Ievl'Ic r+/ c:uh+~tY henu+Rluhin, whith can be dcrcrrninc•J very atcuratcly, will rcnrtt thr 1I

dilfcrt•nccs Ixtwccn Ihe amr+unl of trm,kc cr+nlict with Ihe alvcr,li invulved in scvcral dil)crcnt lrrtntt of Inu,kinR Work also is hcinR sulqxrrtcd wilh the r,hicctive (d learning sonic- thing about Ihe eflrcls r.f nicrrtine tin the cerel.r.rl crrt'ulatirrn A Icchniyue of anRioRraphy has been developed whereby it is possible 1n take what amrnrnts to x ray "movies" of the hkxxl flow in thc cranial vt•sa:+It includ- inR the srt-c:rlled "circle of Willis," the structural didrihutir.n syatcm that mnnitnrs regional cerehral arterial flow. This technique has alrearly shown its value in diagnosis of circulatory obstrvclion. 'I his and other studies are supplcmentary to previuus inrlicatinns that nicrrlinc may have stimulating nr, in some ways, tranyuitirinR ellccls (in the central ncrvr,us systcm. Cnnf.renr• on ('.rrbrnl F: f'.rf. of Nirorlne A conference on the eflccts of nicotine on the central nervous system was held by Ihe Scirntific Advisory floard in New York un Sept 2R, 1962. Wurk under way in Ihis area and sqccifrc topics in need of furlhcr research were discussed by prantces and others investigating this particular fich). In addilirm lo mcnmtx•rs (if Ihe T I R(' tlafl and of the Itoard, the following were present: I)R A K ARMITA6F, KinR's l',dlrRc Iln.pit:rl Medical Schrxil, lon- t)on. FnRl:,nd. I)R lil)WARI) I" I)OMINO, Thc Ilniversity of MithiRan Mrdical Schrxd, Ann Ar)xx. I)R. FSKII. 11ANSSON, Onivcrtily of California Medical ('rnter, Ios AnRrlr. DR FHPI? ('(1RT1S 11Ot F Ar.n T)R. CIIARI IiS H(X'KMAN. Medical ('olleRc of VirRinia, RKhmond DR PAlll. S I ARSON, Medical ('ollef;c of Virginia. Richrnund DR FhW/1Rh W. PFI.IKAN, Iltnton University Schcx,l nf Mrdicinc hR FI C - SARF.t I I, (7ricaRo Medical Schtxtl, ('hicaRo. III 1)R CARI C. SFLTZFR, Ilarvard Ilnivrrsity, (brnhridRe, Mats DR f)tIANF (: WENZII , Univrrvty of Kans.., I awrrn.e Studlr. In rlhe clrrufnrory Arcn New sludies now heinR supl-+rtcd in the Rrntr:tl cirt'uL.tlr+ry area are cnnt'crnerl with vasuular retlwmtcs Irt Ir-haccn tnutkinR in Iratirms with vascul:n th.ra~e, thr relcatr 0 c.1trr hrrlaminrt hrnn Ihe iau)alyd hrart. Ihr rll. , 1 - ,/ ni~ ,,,u,r ,nJ rr t,,- .I .,,1• r,n, r„n .,n,u,r I,'.cl, in Il,r nrrvrmt i A study was undertaken to investigate thc contn•versia yucslion whether I)uerRcr's disease and general peripheral vaa-ular disease are scparate clinical cntities. Research done un+kr a T I R(' Rr:rnt led the atnMas lo cr,nchrde that they are. The study investigated Ihc IlucrRer syndrt+mc in Palicnts, most nf whom were heavy smokcrs, in Ihc Orient, whcrc it was fnund Io occur with relatively high freyuency in Japan and Korca among f,crsuns of low socio-economic class, and in the l)nhed Stairs It was found that those already afflicted with the BucrRer syndrun+e were scnsilive to tubacco, but there was no implication that a similar sensi- Iivily occurs in persons with general peripheral vascular disease Multiple factors of undetermined etiology seem to he involved. Work in this particular field is continuing with a study of IluerRer's disease among various Jewish ethnic groups in Israel Sonic scientists have reported that, for some still unknown reason, the disease pixars to afflict male Jews more than those of any other race. Of interest in coronary research are the several studies that have been previously refxxted on the relationship between the incidence of heart disease and stress One survey covered 1O,(100 physicians, dentisat, and lawyers subdivided 3ccording to the relative stressfulness of their special- ties. Its author, a cardiovascular sixcialist, reported that emotional stress arf+eared to he an important accekratin6 factor in alhcroRenesis when the diet is relatively high in animal fat. Other researchers have relxxled finding an association between coronary disease and emotional stress anti strain. The investigator is alxrut to underlake a similar sludy with stock market workers under a T.I.R.C. grant. SmokinR nnel Prof.in Metnbo/iam It has long been known that young animals esposed lo tohacco smoke or treated chronically with nicotine, gain weight at a lower rate than nnn-e><posed controls. If pnNcin access is adequate, survival nf the animals is not impaired. followinR rclx,rls from F.nRlantl that rats to esfx,sed eRcrNe rmue nitrogen than crtnttrds during the dutation of the cRfxrsurc, an invcslipatinn was heRtm In determine whether an analaRttus cRect call be trhservcd in healthy, younR hrnnan suhjecls Cin.Nr an.f /'.v. An l'Av.iulntlrnl Fnrfnra Of cnntinuinR inlcrctt to Ihe S, icmifit' Atlvisury Ilrrird anrl tn r,thcrs is the rclalivrly unc.ph,rcrl prulhlcros of Ihc varyinR ;tnd Jttlrrin/. t h.rr.r, Irr itlics nf Ix•rqdr `rnrtrr. Irtythn/r,Pical and Irhyti„Irq•.u:rr an,l thr h,.urnK of such ddlcrrntct r,n di.c:r.r Prrrli.lrrtilirm I1

It is now rccnRniretl fnvn a number of studics here and abroad that thcre are mcasurihle or dclectahk dillcrences hr,th among people whr, arnr,kc in v•rrious ways antl betwcen thosc whrr tlo anrl Ihnae whrr do not vnn,ke. auFpevting that smr,kinR practices rney in part rcllert cnnslitu- Iir,nal f:rctr,rs that may influence different disease predileclions One ttudy of male patients at chest clinics reported that thc rersrrn- ality traits of IunR cancer patients diflered significantly front thnse nf cancer-free l+ersnns. A T I R(' -supf+nrled study of a biologically nd culturally homm Rencous group of Italian-American factory wnrkers, almost all born in this cr,untry. found that the kaner individuals smoke significantly mrrre than the str,ut or fat, hut not muscular, nnes. ('ontrary to other previnus rerur,s, the sn+okcrs in this Rsoup were no less maxuline in phytiyue, no more acuve, and cr,nenrned nco more akohrd than nnnsmr,kcra Anothcr cOntinuinR study is seeking to determine ur define lite prc curson of hyfxrtention and coronary artery diseate in a itroup of medical schrw,l students Reports already have indicated that a familial factor is involved A prosrective phase oP this long term prnject is the analysis of figure drawings obtained from the students It is believed that thcce fiRurc drawinRs may provide a simple, eflective, psychrdnRicat screeninR device for Ihe personality appraital r,f normal suhjccts Also continuing is a stu ly of the psych<dr,Rical, cullnral and hcilth ehiracrttictics of aging tmr,kcrs and nr,ncnurkcrs Another invcvtipatinn just beginning will scek lo exansine lite e><tcnt Of and cunaitutinnal haait frx diflcrences in taale untitivitict, and their I,r,ttihlc cnrrcl:rtir,n with any cnn.titutinnal prcdit)xrtitiun to palhrdr,Ric cuntlitiont, with dillcrrnccs in smr,kiriR practices and with frKK1 dillikes Also RettinR under way ic a sludy inur pcycholoRical influenees uitrrn the translation of motives into action with smr+kinR behavior as an inslance. Cls.mJrtry enrl R1orR.mlrrry 1 fre Scientific Advisory Board awarded a grant to scienticls . I Ntath Carolina State College to measure the levels of arsenic ahaurfitiun by tohacco plants in relation tn soil cnnlent of the element and U, tstimate the residual levels of arsenic in the soils used for tohacco RruwinR in varir,us parts nf the state. 71rt study frnrnd that anenic in snils tanPrd /Yr,m I/rr S f,.rtt l+er millir,n (l,l,m ), with an average for all areas rrf the state of 7 X hl,rn 'llris value ii clo+c lu the 4 pf+tn ref,<,rted by 1 1: (;rravca in 1101 1 1„r a vitlvn soil anrl thus +uyPrs/t that there has l.ten n.. nleTlnral,Ie inr rcaae frr,m the I,rrvinu1 u, nf ar.rnitnl ini,c(tuidea in IFhr ar%enic c.mlrnrs r,f auil in wln, h " i. Rrt"n '1hc invcstiRators reported arsenic in the cured Ic.rf uf luh:ntn Kr„wn in these srnfs was from 0.5 to 3.55 ppm, with an avcraFc (r,r all Icaf tamldcs of 1.5 ppm Also under T I R C. support is a study designcd Irr sce whether various annrunts of trace metals, including atsenic and nickel, when added to tobacco, will have any effect on the incidence of pulnron:rry ademmras in Strain A mice that inhale the smnke front such tobacco ('ontrhl .tudics will administer the same metals by other routcs. Another supported project will attempt to identify individual poly- phenolie compwnds in cigarette smoke and to estimate their amrwnts Where feasible, these comfaronds will be prepared for future or concurrent studies on their metabolism and possibk effect on human I,cings. I:PJrIt•mJol..Rltnl Aren A study is now untier way into the influence of certain envimmuental factors in the genesis of neoplastic disease in tuberculosis patients and in children. Tlrc work is being done by four California scientists who received a grant from T.I.R.C. to augment their eurrent project uf testing Ihe hypo- thesis that medical uses of radiation are associated with an increase in the incidence of kukemia or other malignancies. The T.I.R C.-supprsrted project will seek to study the incidence of lung cancer or other cancer in tuberculosis patients with a dcsiRn that would atcertnin the relalionship, if any, between lung cancer in .uch p:rticros and three facUxs-tuberculosis, cmokinR habits, and radiation eagxrture Alur under way in Massachusetts is an epirkmirdugical IunR cancer study designed to check a previous investigation. lhc invcsliRaturs fr,und in earlier woJk that there apf+eared to he a statistical corrclatirm between lung cancer and cigarette smokinF, respiratory illness, heavy crmvuml,tion of akuhol, and uuttlocsr oeeupaliun They said that whilc thc st:uimic:d correlation between lung cancer and cigarette smoking was slnml;cst, lite data !uggeated that a threshold in consumption had to he I+asacd befrue a linear relationship occurred. The dala further shawcd, they rryNnlcd, th:rl the same level of cigarette use with rrddirirrn of pipe or rihnr trnokinR dinrin• ished the disease associatinn, a tesult dillictilt to intcrpret LunR Phy.J.rfnRy Certain phnses rd lite aclual mcchanical wnrk rd lite hum:rn 1unr. renrrin a mystery lo icience (hte .uch rhase is irubn„n:uy clr.u.mce exactly how drKs lite IunR remove inhaled f,articks A stu.ll m,w undrr way in this suhjrcl deal. with the pIragoeytes fuund rn the I,ulmr,nary alveoli. IS 1.1

lhe work heRan with Ihe cnncept (if nrraturinR the ovcrall IunR clear- anct• capacity <d healthy, intact rahhilt RaJrrraclivc srlvt•r nKlidc• I I11, ( whi(h it alco used by mete<nulr+Fitts to secd chrudt for larrlut lirm td rain) was inanKluced into the IunRs of the rabbits by a m n inhal:rlirrn pmcedure lhc rnuvenunt of the radioactive element was then traced with sensitive measuring equipment. It was f<wnd that eventually all of the 1-111 was engulfed by the alveolar phagocytes and carricd Irt Ihc alimcn- tary canal whence it was e><creted. The current phase of the study erKompasses the inhalation nf whole cigarette smr+ke following introduction of the 1- 1)1 in order to rlc,t•rmine the overall eflecl on clearance rates and the relalive rates by alternative routes. Other nimals are to he used in the projrct in adrlitirrn In the rahhil, and druRs that aflect the flow (if mucous iltn arr tn hr invcar~atcJ, as well as the ef/cclt (if other inhakd Rascs and acrnsuk. Afrv..fl Srurlir.t fn Pufrnr.nory F:nrphr.rma Recent ditcnvery that a powerful surfactant tnhstance lint•t the alverdi has gone far toward etplaininR Arc hitherto mysterious physic'aI character- istict of these structures and their cehavira durinR espansiun and contrac- titm Morerwer, the effects t+f Ihn apcnt and the crrnscyuencct of its tlcfic'icncy tupFctl that a hirchcmit•rl appr<tach to the invt•.Ul;:rrirrn (if the etinlupy <d rrnphy%ema may now ht• within rcach A prr-jccl hat t.ccn cf,rmtrncd in which lunp tittu.•t rrhluinr•d at curp.ery nd at lrutr,fxy are heing studred 11 will attcmpt 1<t ruca.ruc the rctative activity of thit tutfactant rccrrvcrcJ frrrrn lunpt allliclcd with carcinr+ma, ernphytcma, and hrnnchiccta%is I Ilrnt will hc ru:rJt• tn dr<r nninc whcthcr tobacco sm<tke inhalalinn :dlccts Itte activity rd this surfactanl, and furthcr study of its chemittry will fx undertaken. A report by a T.1 R('. grantee in 1962 said that bronchial distutb- .nces may pnxlucc an ohstructive airway mechanitm IcarlinR to air trap- ping as well as indirectly inllucncc surlace tentit,n phcnnmen:r in the lunF or impair clearance (if entrapped pollutants In this way. Ihc rc(Kxl sairl, ertxion (if the alveolar nrcnrhranc is initiated I A comparative study of the effects of 7-mcthylchnlanthrcne and ciR- arettc smuke cunJt•n<ate applied to the oral tissues (if Swr.s nucc in wMth liver danrape has hecn previously induced. T he purificaliun and structure tkterminatit,n of the cfdor<rgcnic acid Isomen. Mechanisms of the psychotropic effects of nicotine. Fellrrwrhip ProRrarn In 1962. as in every previous year since 1955, the T.I R C , upon recr.ntrnendation of the SAD, pxovitkd funds (ur the granting of fellow- ships lo the nation's accredited medical and osteopathic scht>,dt Nearly (.(K) young men and women have so far received fellowships. 1)eans uf the schools choose the fellows, and the studenlt, working with their faculty advisors, determine the subjects they wish to study dur- inR sunrnxr or othcr ufl term time. '1'he felltrwship pnrRrans secks tu stimo- lale interest in basic research amtxrR medical schrMd ctudents and any subject of study may be sekcted by fellows, without regard In its relcvanc; to tobacco use or effects. Sunrnrnry 'I his narrative section seeks only Itu indicate hriclly srnne uf the rmorc recent findings and trcnds. More compkte delailt will he frrnntl in previrrut Annual Reports antf in the abstracts they contain Work in ruany <rl thctc ficlds is continuing to receive support. In closins, it seems appropriate to reiterate the hasic belief umlrrlyinR the Advisory 1loard's pcxilion, which was fully e>tpresscd in the floard's report two years ago. Tlrat report ctrocluded w~th lhit paragraph'. "As the tobacco industry continues its suppnrt of the scarch for truth and knowlcdge, it must rcctsRnisc, is is always the case in true scientific research. there can he no promise of a quick antwer. '1 lie inqxtrt:rnt thiuR is to keep on adding to knrrwlcdqe until the accurnuLNivc factt pruvide the hasis fta a srrund cunclutirm " nthrr /'r..)rrfr Antt.nR the other prnjccts, whir h arc unJcr way in institulions tT tn thrnuphtrut thc c<runhy, arc'. A sludy rtf the rlatal In<I ph.uVnl•cal h.rr Irrral Ilnra rrr ~mrrk'rt• rurn m n U. u r tmrrkrrs nnd fnrrnrr snurkcrt I hr .rn. l r-Ui~6~1•nLrr rnr. irr Inincvy lunt• t'inim% :r. iIctcrnunrd (r.mi I-ntmn1h ui nl,.rrv.iW~n~ I7 I 6

Abst ract s of Repurts I ach rcciprcnl of a lr+h.rcco Induslry Rccc:rrch ('ommitlcc Krant in- aid is resl+r,nsrl,lc Mr the initi:rl prescntatirrn rrr publication of thc results of hrc rcacirch in .ctcnlilic nrcclings r,r in apprul,rr.rte scientific juurr,alt. I r,IlnwmR irc ahmracls, approved by the aulhors, rrl rcwarch reports acknr,wlcJgmR cu(.f.rul frurn Ilrc 1 I R.('. Ihat hnve appcarcd in scientific jr,urn.,ls smre thc 1961 Rcptrrl of the Scientific 1)rrcctnr lhcsc ,hwracts have been grouped under the following headings: 1. Studies al the ('cllular l.evel; 11 Pulmonary PhysioluRy and Anatomy; III (:rrJirrv,nculir Studies; IV. Psycho-Physn,h+Rical Shrdict; V. lobacco ('hcmislry and ItnKhcmistry; VI. Pharmacology. VII. OIlxr Studies; VIII. Revicw. I. Sr-rr(lier at flre (:Pllrrlrrr Lxrt'I "INI)ll(-TION (1F ( IInN(7FS IN SURFA('F. /4('fIVI'IY O1 SIRAIN L( I 1 1 SA I GAS MFA/14RANli INII RI A( IiS" Fly Philrp ('tx.per, Ircnc Gr,IdrinR :rml Mrrrrk Klcin, Ikharlmcnts r,t Surpcry. Albert liinslc•in (,dlcl:c ,oI Mcrhi ine ind Ilfnnu Vc,crans Admini%lratiun Ilrnpital, New Ynrk, N Y Sr rrru e, March 20 1962. p:,gcs 725-726. ( 1 I R('. grantee: ('rK,lwr / 1)urinF an invcatiRihr,n r,/ the cttccls rd various Fau„ut aRcnls on the grow,h r,f sUain 1. anJ Ilel.a ccllc rn rcll cultnrc, the itnmcrliate direct cllect of the he«.,Rc if harUCUl.rlc• and non ft:nntuLur g:rsc•a uvcr the curfarc uf thc%c cell%, m a Rrr.e IK•rhrar,n ch.vnl.c•r, w.rt nf..crvcd lhc I:r~cr,u.:ncents irnrluJcd srn„kc Irr,nr Ihc c,mrhu.Unn rd t iC.ucrlca, rd crl;arcllc rr,haccr, rndy, r,f cig.rrctlc hirc•r, anrl nl r,ni„n.kin Lelv t, :uul nrrn .nu,kc F:rsts such :rs eartwm m,mr,tiJc, hydrr,Ren -ml/idc. rllumin,,ting k:rs. r,o~yhcn, aud hcliurn. Imrnedi:rtcly aftcr Ihe injt•clirm,-f all 1•:r.cs Ic0cd tlrcrc ia a very r:q+id incrcavc of the sur/acc area rd Ihc ccll :tv well ;rc.mcrca.c•rl I;ranul.uity Mrrst evidcnt, howcvcr, is "blchhmR" of thc surfacc r+t thc cclla, which is mrrst matkcd near the center r,f the huhhlcs ul gas and is nrN seen nn the surfaces of ncielrtouinR cells. TTre "blebbing" Persists as Iung as buhhles of gas remain in the per- fucir,n chamtxr If the cnntaminaleJ meetium is repl:rced by fresh medium within I O to 15 nrinules :Jter cnnt:rmin:rlion, the cells :uc prr,nrpll,v restored to their oriRinal apl.ear,nce If "hlchhinR" is alh,wed to ct,nttnue fnr more extended peritxls, cellular activity Rradually decreaces and the cclls die. "IllchhinR" docs not r,c'cnr when cclfs in a ch:rmhcr withnul mcdium are cxla,vrd to air or rncdium ahrnc, undcr vrrirruc rlcyrccs of incrcacc'd prcssurc. It il+pears th.rt the "hlchbinl!" resulls hurn a rli.turhantc r,f tL.e .rrl gcl rcl:rlir,n0hrp of Ihc cell .url:rccs and alqatcntly can rKcur at a K:r% nrcmf.ranc inlcrt,rc'e "AI 11•.RAII()NS IN GROW111 ()l SIRnIN 1('H 1 S fXl'( )SI 1) 1O SM()KIi (iASI:C" Hy Philip ('/N,IKf, Morris Klcin and Irene 1'. Grddring, All,crt l rmtcin ('MlePc of Medicine :urd Hrrmx Vctcrarra Arlminisvati,m Ilo, l-rt.r1, Ncw Ynrk, N Y I'rr,rer•,h.rAt rrf rlrr 1,arNV /", !,(rrrrrrrenr,d Ilr •I, ti•r ,rrrd Afrdrr mr, Vr,lnrnt• I Itl, I,npca I I 11, May 1962. 1 he present series of studics was undertaken to clucidate further the direct actir,n of smokc on living cells maintained in a cullure mcdrum lhe lest suhstances included smnkc from the cumhusuon of ciRaretles, ciRarette tobacco, cigarette paper, onionskin paper, and lubacco wrap(*~d ciRarettes Smoke was collccted under sterile cnndilions. The culture O:rsks were in- vertcd and (he prescribed volume of smnke was intnahrced into each Oask. Tlx ftasks were re-inverted, the fluid medium was allowed to wash uver the cells, and the flasks were gently agitated until all smoke was atsrrncord by the medium. Control cultures were treated in e.actly the same manner, e><ccpl that they received similar doses of slerik air. 'I he eRecl uf non-smoke Rases, in- cludinf illuminating 6as and carbon monoxide, was studied under similar conditions. Under the conditions of this study it appears that there is a"loeic" effect of smoke gases on strain 1. cells in eulture This results both in mor- pholupca'. chanRes in the cells and in alterations in growth curves, as indi- caled by the reduction of cell populalions. The smoke (rom the cumhustion of either cigarette paper or onionskin paper appearrrl to Ite more "tonic" Ihan that from the ecxnbu.tion of whole cigarettes or cigarette tobacco alone. Smoke from paper contained mtxe particulale matter than smoke from cig- arette tobacco or the whole ciRarette, suggesting that the particular matlcr present in smoke gases may be of siRnificance. "('t11.TIVA'TION OF NORMAL ANI) MAI-ICiNANI' f IUMAN LUNG l ISSI tF. 1.1 IIE I:STAALISIIMENT OF TF1R EE AI)1'sN( X'A R('INOMA ('lil.l. S1 RAINS." By Mary V. Reed, Ph.D., and George U. (icy, M/), hinney-ltowell Cancer Research l.ahtxalory, Department of Sur~ery, Johns Ilopkms llrrspital, Baltimore. Md. LaAororary fnvexrignrrrrn, Vr,l. 1 I, pa6es 6JR-652, August 1962. (T.I.R.C grantee: Gcy) 1 he ultimate goal of these studies is the eslahlishrnc•n1 uf autolo6ous strains of normal and malignant lung epithelium for subseyuent comparative sludies with each other and with embryonic lung cells in regard to cytologic and biochemical characteristics and responses to viruses. Specimcns from 41 human easea provisionally diagnosed as hrnR car- cinomas and from 1 I human embyronic lungs were cultured in roller lubcs on collaRen, in pbsma clot, and on the Rlass surface with different cuncen- Iralions of uncupplemenled human serum and of senrm fortified with syn- Ihetic media: Nurmal bronchial epilhelium gave early respt,nses with healing cilia surviving 2 to 3 months. Numan carcinnma cells from the lung possess metabolic advantages over their normal prnlolypes as determined by their preferential sutvival and growth in ctfntinuous culture Strnmal dominance may milittale against such tesp.nsrs arrd often accounts /ur failures to ohlain cither nrumal or rndiRnanl cell growth in continuous crdture, The three human tumorous t'I+ithelial eetl slrains dcacrihcd in this paper are in fhe secund, fourth and fifth years rr.pecfivcly 1 wn wcrc ea tahlished frum pt+rty differenlialed ademrarcinumas r,f the IunR, and une was a fnclastatic carc'inuma of the breast trr the IunR liach strain has diq tinctly differenl cytuloRic and Rrowth characterisrics. IH 19

7)thrr Rrnntnr.: Amcrican ('anccr Srrcicty and Natinnal (':utccr In.titulc. "1 11 (i('lS O1 Slil.l ((IVIi III IRAVIt)1 I: 1 IRRAI)In 1It)N uF '1IIli N(I('l.Fl O1 I IVIN(i ('F1.1 S" Ily PO'11 Muntgrtmcry, I Van 1)rdrn, 1. I. Ilumltey, ('. 1. ('hapman and 1 F:. (lxrk, Jhpatlmcnt nf I'a1ht.Jtrgy. lJnivcrsity tr( letas St.uthwestcrn Medical SchrKd, Uallic !'rrarrdinRt n/ thr Srx irt,y low F.Iprrimrnral Rrn6rw and AfrJi(inr, Vrilutnc 111R, pages 172 -175, November IYhI. (7.1 R C. grantce: Montgomery) ('ontinuuus ultraviolet irradiation of Ihe nucleuc (if a living cell pro- duces nuclear darnage. Although this damaee recemhlec nuclea• dantaRe which occors when the enlire cell is irradiated, it is kss sevcre due to the protection of the cytcplasm fronr the effects of irradiatinn ('ontinunus ultra- viokt irradiation of the nuckus (rf a living cell pnxJuces cyltrplasrnic danrage rccemhling that which oecurs whcn the enlire cell is irradiatcd, tmt is less prunounced. "hFFE(TS 01: A('F.lONF. ANI) PNE.NOL ON FSIAIf1.1S111 I) ('1:1 1. I.INFS ('(11 "1IVA 11i1) IN 117 RU." fly IhmaW M Pace and Alice FIIitHI, Instilule for ('ellular Reseatch, Deparlment of PhysirdrtRy, t Inivereily of Nehraska, I.incoln ("anrrr Rrsrarr h, Vrd. 22, pages 1117- 1 I 2, lanuary 1962. (T I R C. grantee: Pace) Acetone and phenul, whichSre common cnncfiluenls of ciRaretle anroke, have been atudird in various crrnccnlrationc with reference Itr nceir shrat- lerrn e/lects rrm mouse fihrnhliet .nrl htmran akin cells cullivated in vitru. ('oncenlratinns rd atcronc. I11 tl rnR'ml and aMrvc, arc trtRrc trr both I~'pcs of cclls lhe akrn crllc arr n~~rrc •cnvlnc tr. atcamnc rllctt~ tlrnn are thc fibrnhlaat c'clla Althr-uRh t rt rny rnl .n r Innr r, nnl tr-trt Ir hhrtrl.I.tUt within a Ill day Lctiral, rt r% n../"„ J.Iv - r.- thc ktn tcll. Phcnul in cnnccntutlnons arl It -` rn' rrrl rnA .rhrovr ro, a rv ttitrt and krlls the cclls within an hour trr Iwtr I,, ,t nrcnu.rtu nc u/ 1/ 1.rn.l 11 It, mK nil srane uf the lihrnhlaal cclls wrrc .trll IrvrnR :rttr r 14) tla~., hw a Jrhnilc inhihiurry rfiect was irrnccalcd r,rrly IS^,;, trf the ccllc aurvivcd for that l.rrio. '(hc ckin cells appeared lo t.c Iccs ccnarlive: 57'.':, trf Ihc cclle aur- viv[(I 10 dayc after exlxrsure to !) 16 mg/ml rrf phenol concentrate. )thrr Rrentnr: The ('txrrer Ftrundalirrn, through the lJnivcreily of Nehraska Frwndation. ""illE? EFFEC'1S OF SULPI1l1R 1)IOXII)F, UPON ESI'AD(.ISIIFh ('li(.I. I.INGS (lJLTIVATF.O IN VITRO." fly )ames R. "Ihnrnpsnn and 1)onald M. Pace, Institute for ('eNular Rescarch, llnivercily rd Nel.raska, I incoln ('unadran lrrurnal of Rrrwhrrnitrry onJ l'hyJirrl.rRv. Vrdume 411, pages 21/7-21 R, February 1962 (iacetrus wlphur ditrttitle has been cilrrl ns rmr td thr nrejrrr cnntami nanta trl the air, yet vcry little is kmrwn ahtrut rl% mtKlc o/ atlur.u u/Har living tiasnr othrr than as a"respiratnry uritant " I hr fracihilrty trl ulrlirrng tisaue tufturr mrthrtilc hrr Irrng tcnn studics (if tlrc r•Ilrttc rrl luw ti,ntcnualirrns rrf ait l•rOlur:1nla ulwrn cclla waa drmrrnalr.rlr 1 F,y Ihr rr,l-aurr Ior Sf /r anrl irt t.rlla ~A rnll~nr. nf Crr.un I nnnr.c trtl, /lrl+r~~hl.t.ta), rn-r.r hvrr trlla, ."n'l 11, 1 .1 ''It, J, tr.cd Ir.mn :rn (I,rlhr•Ir.r! ,.u,tnnnr.r rol Ilrc Irutn:rn tctarx. I lhc Ilcl.a cellc were more sensitive to S(/. cnptrcurc than thc mouu ccllc, wluch ccemc to suplw,rl the general tlKais that Iwm:rns are murc xnsi live tu sulphur diutide than other nramntals. ('ellc cultivated in biohvgical medium grow in cuncenlrations of gaseuue SO_ up tn N/IN) p p m., although somewhat inhibited. ('cllc snh''cc1cJ to a conccntralion af SIKI p.p.m. in this medium are nol greatly aRec,cd and their grnwlh is comparable to those cells in control culturec 1 hc addition uf various salts of SU_ in concen/ralinns from 10 to 2(X) mg % pnKluced reslxmses ranging from complete inhibition of growth (by 2(N) mg 9'o, NaI1SO,) to apparent stimulation of growth by some cuncentrations of Na,S(),. "INFI.I If•.NC'li OF NI('OTINE ON CFUS O1= RAItBI1 S' AORl'A ANh MYOCARf)Il1M IN TISSUE CULTURfS." Dy Tatsuo Kokuhu and C). l. Pollak, 1)over Medical Research (.'enler, 1)c,ver, Ikl. Fl i prrimrntal and Mnlruda. I'tuhrrluRy, Volume 1, pages 293-303. June 1962 (T,I R('. grantee: Prdlak ) llre aaa.ciatitm of cigarette smoking with eardiovascular diceases in several statistical atudies prompted study of the eflect o( cigarette smoke amdenwle and tobacco constituents an cardiovascular cells in vinn. lhis paper concerns the effect of nicotine added to nutrient media be- fore innculalUon or after growth of aortic and myocardial cclls from normo- and hylxrcholesteremic r•abbits. The concentrations of nicatine ueed were arrived at by trial and error. Solutionc weaker than 1:411,(NN/ added to the nutrient before inoculation were inefkctive; solutions nl greater cuncentra- tion than (: I,0O0 proved too strong; other tests were at concentrations of 1: 2,S(11/, 1: S,((K) and 1:10,(KX). Addition of nicotine to the nutrient before inoculation inhibited cell growth, reculling in fewer positive cultures, nd there was severe and often fatal damaPe lo the slruclure of various eells. Inhibition of cell growth te- flecled by the number of positive cultures, deprescitm ul ccll proliferation and multiplicalitm, dama(te to cell slructure, end the speed and degree of rectrvery from damage all mirrored the cuncerHratitm of nictrtine applied Character nnd extent of morPhringic damage as.,well as recovery fnnn st-rne derentkd also un the cell type. I.englh of cell exposure to nicotine was a strong factor in recovery of eells. 'Otl.rr Rrnnrnr: National Ilearl Inclitute. MORPIIUI MIr'AI. ANI) ('YlOILO(71('AL. ST(11)Y UF 111./MAN AMNION (TI.1 S IN TFIE CY)tIRSI: OF RI;('OVfRY I ROM RAINA l1UN IN1tIRY." Ily Maaahim Mizulani, Yuh 11. Nnkanialhi anJ (' M Pomcrat, (Inivcraity trl Te.as Medical Branch, (ialveclun 7rrur Krporrr rn BiohrRy anJ Afrdirinr, Vu(. 19, pages d11-N24, Winter 1961, ( 1 I R(' granlce: Ponrerat) A well ratahhshed human arnrnrur itrain which wac cuhivarrtt m Rtnr thaml.crs w.ra uradratcd with ItNK)r Irum a ctrhall" %ooltr lirr m.rtcrr.rl. Ihuc lUentr•d wcrr slaincd wilh lacuhcun's rnclhrrd daily rtrr fivr auttrt.rvc dayc /rdbtwrn); irraJiatiun 'Ibe changes in cell Iwrpulalu,n an.l nnrtrlic mdrl and Ihr inciJt•nrr rrf granr and mullinuckale crllc were exaunnvd A lrr-/rul.t IirNi u/ thrc .trrur wluth Irad a mutl.tl ehrrnnrnuure numhrr td /S .hnwcJ severe injury altcr irr:rdrauun lhe crll culturca Krnwn Irrrrn thc rrtr.vrry 21 ,n

rrf r:rrli:ucd ccll tultures wcrc ch:tractcri?erl I+y a rnrwlaI chnrmi ,o nnc numhcr tit 71 l Fhc mcr hanisnt rrf giant ccll (nrmatinn ind thc ch:rn/-c•, in the mrKl:tt clhnuurr.rrnrc nurnhc•rs havt hcern rlt~tusscd in rcfcrcncc bt rcfwrrtcJ cvtdcnce. hrr Rrnnlnrr Schtwrl yd Avialirrn Mcdicinc• IISnI • R:rnrLrlph Air frrrt'e 1 c><as II, 1'rtlntrrnnr-y 1'hyaiultrRy urt.! .Irnnt.rnty "PI(()1 t(' I I V I. 1?Fl li('T OF PARASYMPA 1 III I I('(/MIMIi-11(' A(i/ NIS ON ( II.IAl1:1) Ml/('l1S•Sf:('RI.IIN(i 1TIII111 1tIM." Ily 11:Im1. I:tlk, Ph I) , Paul Kotin, M I). and Ilcrta M. I rerner, 1'h h• Ik- partrncnls rd t'ahrdtrRy, l)niversily tit Southern ( alifurnia Schtwd (rf Mcdi- cinc and I rn Angeles (•rrunty (ieneral Ilrnpdhl /r/nrrrnl tit thr Nurinrnol ('nnrrr Intrirurr, Volunx 27, pages 1179-1192. Ikcemfxr 1961. ( 1 .1 R('. Frantcc Falk) Fxprnurc tit ciliated nwcus sccrclinR epithelium of Ihc e%rrphaRus of Ihe IrrrR Irr Lar~symf~albcticnmimelic a6enls prulccls aPainsl 1hc cllccts (if irrit.ml acrr,vd% %n that the dnwinR of mucous slrcam induced by Ihc Iallcr drres nrrl tx'cur 'I hc aRent( studied inctude I ) acet)•Ichrrlinc and cMdine like drnr, 2) chrrlmcstcrasc inhihitors, and 1) comfxwnds rel:tlcrl tit nicr.linic acid f'rirrr af,ldicalir+n r+f such.f.arasyrnpalhelicomimetic aQcnls eacrls a prrrphylactic eller:t aRaind the suh.cqucnt action of irritants. Irril:rnrc fmwnt in the parttc ul:rle phase uf ciRarettc smokc and urban rtllutcd atr inthrce a shrwinR in thc ratc rrt /lrrw in the mucuus slrc:un, proha- y as a rccult of altcralirrns c+f cilt:rry activity artd nuwliticatirrn nf mucrtus secretion !n rrrn this h:ts hern ahrrwn Hr l:urlttalc PrnlnnRcd relcnlirm nf r:trliculate nrattcr tettliny rm the re~l,ir:ttnry cltithcfium of mice. rat., and rabbits Whcn Ihc partrculatcs crrnar.t rrf rtr cr+nt:un known carcinrrRenic apcnts, their pt~sihlc rrdc in Ihc Ir:,Ihrrgcnccis trf I,ulmrrnary cant'cr is supgcslcd Iks)ii1c the dcmonstralir.n tit carcinn}cnic, aRcnts in I+r+lluwJ urhnn air and cigarette smoke, analysis of cpideminlop,ical data revcals Ihrt by f:rr the greater rnaj<rrily rd Ihnsc cxPrned lo this carcinrrgcnic rnilicu drr not dcvchrp puhnnnary cancct. lo us an alrraclive and plausihle explanatiun hrr this cpiJcntiuhrFic:d atlcrn is that Irrcat and systemic host factors are siynifrcanlly concerned with the entry and action of carcinrrgenic agents AOt . Arr Rrnntnr.: National Cancer Instilute and American Cancer S(x'icly. "1'ANI.OIfll1.AR f:MPIIYSIiMA ANA1OMY AND f'AIIfUUYNAM- 1('S " 11y John I'. Wyatt, M 1) , Vernon W Ui%cher• and I lr-ncrrl C Swect, MO , I('('P, I)el,ar/nrents nf I'athrduRy and Mcdicine, SI I ouiv I lnivcr.ity Schnrd of Mcdirinc, St, Louis, Mo 1lrvnrrr nf thr (Yvrr• Vrd 41, h:Ipcs 211) 259. March 1962 (T I R(' pr.mlcc: Wy:rtll 1':rnlrrF.ul.rr cmrhyscrn:r is a mrnlrhrdrrpu dcnnminatnr t rrnrnu,n Irr wr h unlike tr+nditirm :rs (+rimary huml+h.ttk thc.t rlr/ramitics, 1ra:rhtcJ h.urlcn inR rrf Ihr thtln. r-f Ihc Inn1•. or Ltrpc hrnnr hr.J ulwav% dc lrunritrt% .rnd trm. pcnrl.rl "L.h.rr" ~ nrl~lry., m.r 1 r a wrll rlrInnrl r, Ihr .rrrw.r,rd,.trn~ Ir~,n in ~~.~.i ~ t.. ~ . ,. . ~ ~... .., . .r i . . ...... ... . . ~~• ,~,rL,.~~i ~.nr~~ lural hranchial deformity was also found, but thcse w•cre usually assrriatcd wilh Icaner degrees of emphysema. 1)ronchial disturbances may produce not only an ohstnrctive airway mcchani.m leading to air IrappinR, bul indirectly inllncnce swlacc tensiun ncenumcna within the IunR or impair clearance of enltaphcd Ixrllulants and in this way iniliale ernsion of Ihe air sac nrernhranes. IIyPCrlmphy of Ihe right ventricle was a frequent cornPanrr~n tit es- lahlishcd cases tit RcncraliYed panhrbtdar cmphyscma. The air Irr tbIrwrtl ca changinF surf:rcc -- the pulmonary contribuliun to homeuslasis - is pro- rrssrvcly dcslrnycd through alveolar sac dicsrrlulion with Ihe cuncurrenl ~res uf the perfusing lung bed being resfxwnihle fur Ihe ctrmrnrm clinir,d manifcslalirrns of cnr pulmrmak. OIher rklerminanls invulved in the develupment of cor Pulnumalc are pathologic hy-passcs from artery to vein, hrunchnPulmratary shuntinp p,rr- Ircularly in enlPhysema with fihrosis and a dectNnl.Cnsating fwrrlal atygrrs rulrnnnary vein syslcm 1hcsc rnorpholuRical frndinRs rcllccl Iltc Primary rmlwutancc tit the Pulrnonary•cardiac discase in the natur:d hisaay rd this form of cmlrhyscma. 111. Cordinrn•sc(tlnr Stutli(•. " I I11: VIISR(X'ARIN(GRAPIIIC fiXfiR('ISIi 1 f?S'I If( )I( (1)R( )NAR Y INSIIf•F1('IF.NCY." Ny (larence M. Agress. M 1) , FA('(', and Stanley WeRner, C'ardiovascular Research f-ahcxalory, University of ('alihrrnia, 1k- partmcnt uf Medicine, l.os AnRcks, ('alifnrnia. Anrerir rur lraernrJ n/ ('ardin- uLrgy. Vol 9, pages 541-546, April 1962. ('C.I.R,('. Rrantec: AKress) l he data obtained front this study represent a snrall Rruup rd fraticnts and, thcrcfure, can only be considered as a preliminary tcyarrt Since the patients selected were a carcfully-studicd RrtwP, huwevcr, Rrcater accuracy can he expected than from mass data colkcliun. l hc vihrrn'ardirrRraphic exercise test rcrfornred is a qvantitative test dependent nn a numcrical rnca- suremcnt only, and is free of qualilative interpretation. Inilial dcllectinns of the vihrr>tardiogram were mcaiurcrl Ixfrrrc and after a single Iwn step test in RI palicnts, including Mrth mrrru:rls and IKr• ums with ruytcardial ischcmia. 7 he nrrrmal reslamse was a widcninP of Ihe wave inlervals• expressed ns a ralio nf total syslydc• the incrrrse rangrng frran S tu 21M1^J~. In abnormal subjects there was a decrease r:rnRinK frrrm S to 61^h.. Nr+rmal and fUnclionally nrrrrnal patients currcl:ttcd wilh their clinicil diaRnrnes in R7^.(, nf the eases by thc two-slcp test and 't4"S• by Ihe vihru eardinfrahhic interval test In :Ihnnrucd palicnlc the currcl:trinns wcrc 6h":• and RR% rc~reclivcly. An addturrn:tl RS cascs inclu,linF hrrth sinplc anrl JuuLlt• twn .tcl- 11.0% Iravc la•cn arlrlyd Irr Ihc sludy with no cacnUal t hanl!r m ncw .rlrrrvc cont-lusirrns "I I I I.('1 (11 NI( (t11NFUN'111F('ORUNARY I11 ()(11) 11 (11V nNt) RI I.A'I1;.1)( If(! Itl n1O{ZYI'AI(AMI:11 HS t(/1(It11 nllii'I S11i1)Y IN NORMnI INKiS AN11 It(NiS W1111 ( uRONnl(Y INC1/11 1('I F.N('Y "anJ' 1 I I 1( ISrrl I'1 It\,\NIIN I1(n,/• A NI \V ( t)l(()NnItl 2 1

VASUUII A'IOR. ON ('ORONARY III (N11) I 1 c)W ANI) ( ARI)IA(' 1)YNAMI( S IN IIIF Ix)(i." Ify Samucl Itcllcl, A11), )amcc W Wcct, Ph I), Otln V hlnllcr, M U. antl FIF(, (•. Manrt,li, M 1), Ihvi.iun (,f ('anli- tdt,yy, I'hrlatJ.•Irl+ia (iencral fltnPital and I)cl+attn+cnl r+/ I'h:rrm:rtt,InKy and Rt,hmctlc I(,undatit+n• llniversity of Pcnncylvartia Mrtlic:tl Srh+x,l, PhilatJcll+hra ( ir(uLui(,n Rrsrorrh. Val. 11), l,a(;cc 27-14 and \S-44, lan- uary 1962 ( 1 I R(' Rrantec: Nellcl) 7 frc cllcct uf inuavenousl~ adminidcrcts nic,rlinc ( 211 rnicrtr- Rramt, KR min ) t,n cnrunary hltxxl flow and relitc(1 hcnurlynamir Ir,rr.unc- Icrt wat sludictl in thc nttrmal inracl tkrR and in the dt+R wilh intluct•tl chnmic ct+rt,nary inaufricicncy. Nicotine increaced cardiac wtak m:uke(Ity in IxHh RrouPt td dt+Rs hy an increase in the mean artcrial blood Pressurc and carJiac tnwrPut. Mean ctrntrol coronary hkxrd flow was mtxlcratcly tkcrcatcd in t1uRs with cornnary artery ligation and markedly Jc•crcaccd in dt,Kt with narrnw- ins nf thc twit main hranches td the kft corttnary artery Inrrca.c in ct,n,nary~ bltxxl flow Prtxlncetl hy nicntine in nunnal dogs was tnc%crvctl Irr t+c ac hiKh as 1257(• o.r an average over the ct+n(rul vr,lue. 'I he averaKc int: reatc wat 8 2 5% with IrKation of one cort,nary arlery branch and 9 3 1^r. with narrnw- inR r,f the twtr main branches lhc res(,t,nte of the cnrunaryafhrw wat tlircclly rclatcd 1(r the tlcKrce of coronary narrt,winR and/ur tKCfu6nn; the grcalcr the imhairmcnl. the smallcr was the incremcn/ in ctatmary hltxxl flt,w, which Irctlucntly tJr(,PPc•J t.cluw the control value in Ihc fx,cl inlu6nn I,eritxl ('t+nrn.try vaecular rc- sitlance end rnyrx'ardial nXyKert uliriruinn tlcclincd during nrct,linc atlmin- ittratitm. I'crsanlin• a ncw ct,rnnary vattxlilalt+r, I,rtxfut:c(1 :rn avcraftc incrca%e in coronary hltxxl II(tw of 15V^S. t+vcr rtnurr+l vafuct in nt,rnral animalt• of 1hSry., in Jt,Rt wilh ligation of trne ct,r(,rcrry arlcty hranch, and t+f SI^, in dogs with coronary artery narrnwing wilhuul incrcatinp canliac t t+ntractiltly o cardiac wnrk. O V se rh.r Rrnnlnr.: 1 he Fili I.illy ('trmPany, the I i(c Incurancc Mctlic:+l Rc- arch I unJ• and the American ficarl Ascocialian. "FfFI:(-r OF C'I(iARFTrfi SMOKING ON FRFT FA1 IY A( 11rS IN PAlIIiNISWI11111IiA1,Lq MY~ K~ARUTAI•INVAR("IIUN"Ityl+I(rcd Kcrshhaum, M F)., Samuc(11r.ikt: M.l)., FACC. Raymond F. ('aplan. M 1)., and (.cnnard 1. FeinherR, Ph 1).• Philatklphia Gcncral Flutpilal, Pcnncyl- v:mia Arnrrr( nn I(,urnuf of ('urdiuln)rr, Vulurne II), P;tKct 2t)4 2I/A, Atrgual, 1962 lhc effecl (,f srnokinR on scrun/ free fatly acidt (1•FA) was sl.rtlied m 1 7 I,alicntt wi/h lrcaled mytx'artli:J inf:uctitm, aKcd Ih h•/, incluJrnK r,nc (cmalc I here w:,s an average rnanimal ri~c of hS f,''S in I 1•A in :JI .uhl(•c1t in 111 It, 2/) tninulrs aflcr tm+,krng Iwn tiK:ucllct irn a It) tninutr I,(n+,d St,nte elcv:rUt+n utually Per.iclt•d It,r 411 nrnnnet Irt Ir, nt.n tnr+,nary P:dicntc I I A clcv:rti(+n. :+flrr .m++ktng '7 2''% •:+n+l m I1/ n~,rm:rl tul+lrt 1% Ihtr avcr.+Pc ,u.+a+n,.+l rr.,• w.r. 2 1 h": In I I n+m "nt•ku+l• (+•nuult, Ih,'rtw," an .,vrr.+l•r n+.,tnn.+l I+"r n1 I/";. +In++nr• Ih+ Ir.t In,n0 II ic tur.rt•ttcd that the greater FFA recfx,nce in myt,(:uJi.rl in):ucu+,n Paticnl% ic Ihc rt•tult of a Rrc:rtcr calccht,larninc rcIca.c a11rr nn(,linc stintul:,tiun U,14,hbrr Rrnnror: National Ilcart Inclilulc. "P11:IAFlt)I I(', IIISI(/1 (Hil(' ANI) I11S1(K'11FMl('AI. ACI'I-( IS UI llIli 1f(IM(1(iRAI 11:1) IIIiARI•" Ny R. 1. alin(t and (fly invilatitm) C. ( hil':r, A('hrytnhun, S GuJhiarnacttn. and 1'. 1.. Wtdf, Waync St:ue Universily ('t,11cFe of Medicine. Iklrt+it, Mich. 7runrnrtit,nt n/ rltr A tart in- Nrmr,rf .lrnrrnun I'Irt'titrdn.t• Volurne 74. P:r(;t•s 1IR-•112, 14h) (1 I R(' Kranlct' IIinF) Mclalx,lic ttudiec on the htrmngraltcd canine heart dcmt+nclralcd in snnrc Prcparatiunt high respiratnry yutNienls caislinR IuRelhcr with iocrcaced pyrovate• negative mytx•ardial kettwK' catractitms, and dimmithcd 1,11 in coronary vcin hhxal. lhe percentage Rluct+te ursyRcn cztraclitm r.ttiut fre t~nently e.cet•ded I(N)'• 'Ifu•te chanret cuRRetted glyealycic• wilh a hrcak- t1(,wn uf pyruvale throuRh the (at(y acid r.Hfher than Ihe Irirarhuuylic acitl cyclr. '1 he rct',ir:dt+ry t'hain, htrwcvcr, appeared hr Fx• inlact, ti-nt'c dif- ferencet in uxitlalitm reduclitm~t entials between coronary arlerial antl vein hltwxl wcre neKalivc. APparenlly, ht,mnRraf( rejectitm resullcd in incrcaced cell rerrncahrlity with Incc of enryn+es and euemymes fYt,m thc ccll 'Ihe Iwtr hatic patholtrRic prt+cescet Presen/ in the cct•lit,ns uf thc hmmo- (;r:dlcd hc:uls were Rranulnmaltxrs rnycxarJitic aimilar lit Ihc :,rutc (,nddcra- live stage trf rheumalic fever bul lacking fihrinaiJ nccnr.ic an(1 myt+cartlial necrtnit after R Jays of viability. This pallern is most likely due ttr an im- mune lictue reclnmce. Ilictnchcmical sludiec demonctrate active latxluctit+n of RNA protein in (he graft and marked reduction in malic tlchyJrtrKt•nasc in the donor ccart after RraftinR. "1 RANSPI.AN I Al ION OI-' 111E 111iART." Hy Richartl I IIinR• ('hiyn ('hiha, Alhanacitx ( hryurhou• Paul 1.. Wulf and SiFmundur (indhjarnaa,n, Ik•Partmt•nl of Mcdicinc, Wayne State F)niversity Cullerc of Mcdiiine and Iiarlnr 1ltnpilal, 1)clruit, Mich Fditorial, in ('irr'rslurit,n. Vultume 25, PaKes 271-276. I:chrirary 1462. frantplantatitm immunity• rccuhinR in dre rejeclion nf the htrnu,Kraf1, it Ihc tcttdl of antiRcnt frt,m the Rraft• which, trn reaching the rcRit,n:d lymph ntxlcc, climul:rlc prtxluclit,n td lynq,htrid ccllt which infilOralc Ihc Krall and dcdrtty it 'framl,l:rntalitm immunity may hc aclivefy.acyuirctl• antl adaptrvc imtmmity to Ihc h,nnuKr:dt al.o hat hcen tkmnnstratcd. Altht,uph I)rc n:uure of the anligcnc ic m,1 known• it has hccn shown that cells c:rn he nnuphtr logically tlicintc);r:rtctl wilhtnrt JcatrtryinR thcir (x,wcr Itr clich Ir:uttld:utla- tiun imnrunity. :u+tl it hac fx'cn demrm.tralcd Ihat Ihc amiKcntc tuh.tantcs are cslx•cially c'+,nccntr:ttrd in Ihe nuclrar fracritm t,f di.i r:Ued ccllc In thic l.th(,ratury. twtr dic.inrilar palhtdt,ytic /utxca.ct wcrc t+l..crv,•d in hunnt)-rahc(1 t:tninc hrarla; t+nc h•tit,n crrntiNCJ t,f a tldfuac I,.urc:nJitis (Kranul+,maltnr. in/iftrati(,n) Ih:t1 wat alrrkinFly timil:rr lu Ilic (,r+,htcr.tlive tlaf•c rd acul(. nccnn+atic Icver Ihc tatxnd type nf ch:urpc (,F,ttr.t•+I w.rt acutc rnytx':uJi.+l urt r(,.ic wirh inrcr.tiual elkma and I,nlvrnt+rl,h++nu( Ic.,r ncutrt,l+hilrc• intiltn+u+un td Ih( ha/rncnlcJ mytx:u(th.r1 ti..uc It r% md I+krly that Ihnrnt+Kr.rfts uf Iht• hc.ut will I,t' nf :tny t linrc.tl i+nl.+,rl.tnt r rn rhk, /+,rr.rt n futurc II(,wrvcr• it r. I+rul,:+l,ic 111,11 nn+c (un(l.rnrcnl:d knnwtrd/•(• (.+n I•r 2t; )t

Rnincd frrms a t:rte/ul cttrdy t,t Ihi e+,rrt•latit,n hc(wccn thc Inr.)•trccitm r,( hr,nrr,yr:rlt rcattnmn and the rnct:rlx,li.nt ul the (r.rncf,l:rnlcd hr.rrt " 11IS. RI I A I 1( )NSIIIP 151.1 WI I N ('ORONARY ISI (xtl) l tOW MYIH'ARI)IA1. t)XYG1;N ( l)NStIMi'llON ANI) ('ARI)L1( W1/RK AS INI 1 tlI N('I I) 11Y f'( RSANIIN " by Vcrnrm I:. Wcnrh, M I), )rihn 1 Suntlrtrnctcr, ft( 1), 1':mcraa 11 tkn Ilakkcr, M I) and Rrth.rrd I IImK, M 1). I A( ('• l),rI,ath+unl t,( Medicine. Wayne Statr tln+vcr.it) (,dlcFc tt( Mcdtcinc anrl 11arl,cr Ilr+cpilal, tktrni/, Mich Arnrnrnn Jfrnrnol tr/ ( rrr.brr/. ,,t,t. V,dumc'1. r:rl'cs 44V-4S4, March 1962 1 hc cffcct r+f n rytimidta-ryrimiJine tktivalivc. Percctntin. 4m ctut,+nary hlrxx) flrrw, myrrcarAial ntyEcn ct)nsun,ption antl cardiac wr,rk wac chtdicd in nine r:uicnts wl(h/xlt C:Ir11MV]K•Ul.lr llKeace 1'crsanlin cauccd a dccrcasc in crrrnnary v.ncul:rr rccictance 'Ihc dicu+ciatir,n hclwcrn t'irrliac wtak and tayrK'ardial my}:cn ct,ncamplit,n sufhcctc that Petcamin aclc upt,n ct•Ilular rnclaht,hsm, caucing on inctrase in the t++t)gtn dcrn.tndc ami nccrccitalinR an incrt•au in ctnrrn.vy hlrxxl Ilnw '1he nrl1ninislraurm nf 1't•rcanlin rccuhcd in a rlct linc in 11x• ratitr rd kfl vcrnricul•tr wrxk tn rnyrt'artlcA rreyrcn tv,n cunrhtir,n 11 ic crmt lurkrl that the dtrtR, like nitrc,Plyccrin, tlnnini.hcc c:rrth.rc wr,tk in c,nnc p:tt,cnta and h:x a dirccl cflccl un catdiac ntctaMdicm "('All ( I1()1 AMIN(S IN It( IAU)1 (KiUIIS IIVAR'1 (iRnl IS" fly A Wt yrrtann, (' ( hrt,a, A('htycMhnu and R I Iling, t)c1+aHon•nl u( Medi- cinr W:rynt• St.rlc tlnivcr.ity ('rdlc~r r,l Mcdicinc. (kttt,it, Mith 1'nMrrd- ingr n/ rhr S,krrrv /.rr 1. tprrmu•nnd ltr,dnt;v arul Afr•Jrr rnr, Vr,ltnuc IIMI, I,aRcc t.ll t4t, M.rrch I'1(r2 ( r,nrldctc dtrn•rv:tti„n t,f hnl,l,y hrattc wac accr,ml,li.hrtl hy hunt,+- U,Itr~uc Irany,l.,nl.rlhm l.uJr:rr t r,urr nt ;rml inlr:rcrllular th.trrhulrnn n( c:AC- thtrl.rruincc rrrn.rint rl un.rtlt t tctl thr• 11,0 ? 1 lhrnnc Inll„wrnp Ir.rny,l.tnl:r/irm Wttltitt Ihit, Irurc iK•rirxl attclitcurrl telctlrun Jrtt nul iotlucntt• thc..rl:clu,l.t- mutc crnNcnt u( IF,c hc•tr7 rtaft Altvt 1 2 lu,utn n,r tah'ch, (.r+rrnrcc t,ruhl I,c dc/cclcrl in Irancl,l:tnlcd hcarl "SSttl>1F.S ON 111t= IRANSI'I ANII'l) III~.AR'I. IIS Ml.lAl((1l ISM ANI) 111S 1O1 ()( ;Y " fly ('. ( hiha. Ai 1) . 1' 1.. Wrdi, M 1) , S( intll,jarna- snn, I'h I) , A ('hryarrhtnr, M 1) , 11. Ramrs, M 1) , 11. 1'caram, M 1) , and R I 11inR, M I) • 1)cparlmcntc of Medicine antl 1'alhnluFy, W:rync State lrnivcrcity („Iligc t%( Mcdrtinc• and (lurl+cr Iltucpit,l, Urtrr,it, Mith Inrrrnul rl/ l tlrrrirnrnnrt A1rtlrrrnr. Vtdume 115, pages R'C) Hh(,, Al,ril 1962. 1 the mct:trxrlic thangcs in the hr,mnFr:+ftcd cnninc hc:ut wcrc ctnthcd in rtrtler It+ deline the hir,chentical altenttiunc accumpnnyinR hr,mt,rraft re- j<t li„n 1 Ix humr l r:r(ted hcart rt•IcacctS rytntvale anrl I;tct:+tc :,c wcll :,• malic rk hyArrrFcnacc :rnrl :r(drrlace I:><traclirm 4,1 ,lucrKc hy Itre p.ralt u.ually re- m:rincd prnitivc In ccvt•r.r1 rtlx rimcnls, hr,mr,l;r.,ft tcjcttiun w:,c accclc•r:,tctl hy l,riut ccn.itri.rlirm tll thc hw.l :utimal 1lurin); the :rtccicr.ttcd rejc•t tirrn• the rrQ•act u( pytuv.ttc an,) I,tt:rtc w,ts ntnrr I,nsnrnmccd, nntl cvcn ylutr,,c :rl,lx•arcd in imtt:,tc,1,,,ntcatt:tUrmc in crwtrrnaty vcin hht,xl In rn.nry .I,rrurrrnl~ Ihr rr•prr.rt.•ry tlnutir-nt,d rhr uan.1,t.nrtt,t hr:,tt wrll n. u. t•lur„•r r~ttl•rn r,trertrnn ratr„ wrrc rlcv:rtcrl It .,rrnk h/,cly that the ctcv:r(ed recrirah)ry quotients were Ihe rccnI/ of thc canvcrcit,n n( c;trhr,hydr.ucc to fa1. 7he rnclatxllic bltxk or hhxks precrnt in the trans- pl:rntcd hcarl arc likely tu hc the result o( diminutitm tt( inlt:+ccllulat rntyuics I a ctKnryntcc resultins (Rt>,n increaxed cc11u1ar pcrmcahrhty. (h.r Rrnn(nra: U S. Public lleahh Service, Ametican Itearl Accr,ti.uit,n, 1it:hrRan Ilr.ul Asu,cta(itM, I ife Insuratxc Mrttical Rcsratth l und, Iiurnwt~bs Wrtla,me 1vnt1, and )nhn A. 11art(urd Fuundatitxt. "MY(K'ARI)lAI- M1:TAIl()LISM IN A PATICNT WI'I11 IlAC111- MOT(YS 111YROIf)171S ANt) IIYPOTIIYRt)11)1SM " Ny 1' Il tkn Ft:rkkcr. M 1) ,). F. Suntkrmeyer, M 1)., V. E. Wendr, M I) . M S:rlhanry, M h., S(:udhjarnasnn, Ph.l)., and R. 1. DinR, M.1). Arnrriron lmn.nal rr/ Afrditini. Vnhrme 32, pages R22-R26. May 1962. A case of Iiashimottis thytniditia with hylxNhyrnidiern is prccrnled ('ardix (mulPut, axt.nary htiaxl flt)w and myt+cardial uxygcn cnnctnu/airrn wcrt within Ihe nurntal range. Pyruvale and lactate were ettratled hy Ihe fx•:rrl durinR tlrc hasnl sule and rekased by the heart rm exrttise. A tnarkcd inc'rcasc in myt,cardial glucnse.axyRen ertlraclit,n ralit+ was (t,und and the diRcrcnrts in oxiulatit.n-rcduclion Ix.ttntial hctwrcn arlcrial and curtmary vein hltxxl were Ix.citivc at rest nnd euercise. The reculls snl;prct Rlycrrlyeis in the cardiac muct'k of this individual in the presence t,f nrrrrnal rnytr'ardial (D><yFcn ucaFe. "Iltli Rlil)t)X P(YI-1:NTIA1• OF Tllr I.A('rAl'1~.-1'YRIIVA'lli SYS• 1FM IN F11 (N)h AS AN INI)I('A 1011 OF T11F 1-1 tN( I I(1NA1. S I A'I F ( W('FI .l.l /I.AR ( )X II )ATION " Ity SiRrnundur ( iuJl+j:un:r.un and Rirlr,ud 1. ItinR. Rirx lnmit a ct Rierphvtitn .4t ta, Volume 611, p;tKcc 159 162. ltute 1962. •1 he rctlr,R-fxrlentials rrl heart mucck, arterial and crattnary vcin hlr>exl havc lx•cn cakulatcd fram the ralirl of Iaclalc/Pyruvale in Ihc l,recrntt and ahacnce r,f myrx•ardial anunia. In absence af myt)cardi:tl anuni;t, the rcdut- ratcnlial ttf hcatl mucck is mtilre rntitive than that t+( attrrial hl,xxl; lltt retlt,x-p)tcntial t,( cort.nary-vein bhxxf nppmaches that uf the ht•:ul muscle. In mytrc•ardial anoaia the retA/>t-(xNenlial of heart nrust'Ie is rnrrre negative than Ihat uf artcrial hltxxl; that ul cnronary-sinus t+hxxl :y,lar,aihcc thal yd lhe muccle. '/ he resulls n( this sludy suggest that alteralitmc in AI h attass lhe hcart rc(lcti paralkl changes in llx mytKardit1t11. FAI I Y A('II) ('ON('F.NTRATION ANI) ('t1M1'()SVININ IN ARIl?RIAL ltllx)1)." Ily Mattin F. Rtxhtin, ('htictine It Rr,thlin and Vernr,n V . Wendl, Ik•patlmcnt r,( Metlicine, Wayne Statc ttnivrr.rly ('rdtrgc of Mcdicinc, I)ctrr,il, Mich Amrrirun Lnnnrtl rr/ 1'lrvnwl.-A•v, Vnl ?I11, pagcc 111h-11(/, AuFusl 1962. ('1'.I R('. Rranlrt•: IlinK) 1 lie cllccl trf the at)miniclraritrn ttt nrwr(+intyhrine, rlucr,.c end in.ulrn, 1.cnlr,h:ul~qal, and II,iIx•rlcncin un the arlcri:d t'uaccnttatir,n antl t,rn+l„-.i lirxt trf placnta ltcr f•ttty acitlc tFI A/ h.n hccn studictt iii nt.tn and rh,g Wilh a riee td Ihr I I A crmcrnualirm as Irtrxluccd by nurclanrl,hrinr• lhe ct,ntrihuli,m trf nlcic acid trr the IrNal I-Fn int•reaced, whrlc IIr.H rd clr.uii and I,alrnilic aculs Jrcreaccd Ihe rrverce chanRee in Iht• I I A cr,rnp,rcrtrnn wtrc tlhu•tvctl whrn Ihcir arrctial kvcl (rll undcr the in/tucnrr td .,Ihcr aRcntt stutlicd 1 hc 1 1 A ct,minsititm was dclx•ndrnt t,n the / I-it tunrrntt a- I 27

r I lirm in irtrri:d 1,14,441. hut not nn the ciF+crinrcntal crmdrtion rrf thr tnhjcct vr anrrnil it the limc of analysis At hiRh I I A Icvcla, thc I I-A c-ntl.rtrturn af~I+nr.rthcJ rhit of .kp.N fat "I IfF. ROI F. OF 'I II1: III: AR 1 IN SIIO('K " flv Richard 1, IIrnR, M 1) , anJ I lcrminin Ramrrt, hl l). l.rurnaf nf the Anrrrnan AfrJna! .I r.nrratinn, paRcs fl)1 R71, Scptcnncer R, 1962. 'I hcse studics have indicated that oxygen lack leads to raprd dtphns- phnrylativn of hiyhcr energy phosphate compc.unds and 1t. Ibc suhscqucnt forn.aUon uf inorganic phosphcxus. In Ihe heart cnnrpletely deprived of nxy- 6rn, Rlyco Rcn aFso Jisapptars rapidly. Alteraliont in the carbohydrate inter- mediatcs tlirring myncardial annaia are also wcll defined. 1 he manifold and divcrse disturhancet in intcrmeciiary metaholism pnwfuced by anoxia cMrld well lead lu rkpression of myocardial contractility in shnck. It is also quite p.nsihle that the dcterioration (if mycxardial expuisive pr)wer cnntrihutes to the dcvelt+pn+ent of irreversihk circulatory failure. "OXII)A1I(/N R1Dl)(-IION IN FfEART MI/S(l.li ltll:ORlill('At. ANI) ( I.INI('At.(Y)NSII)I;RAI IONS." Ry S Gudhjarnas.m. F'h 1) , R(1. Ilayden, M 1) , V F--. WcnJt, M I) , T. 11 StcxA, M U and R. 1 Hing, M I)., F1<parlmenl r+f Medicine. Waync,Statc liniversily ('olleRe of Mcdicine, F)eUnit, Mich Cirrrduriun, Volume 26, pares 937-945, November 1962. llrc purpose of this rep.ut is tro pnint out that changcs in the r+xy(Ccn- reduction Irrdr.x I p.rtenlial (rf a ccrt:rm rcdt.x systcm in the hl.xxl Ixrluaing the hearl muscle rcllect altcratir+ns wrrhin heart ntuscle cells A review of clinical and expcrimcnlal finthngs by Ihesc authms anJ nthcrs illustrales the limited value rnl cilculali.rns tit the st:Hc trf ccllul:rr r.Ridilitm in hrart mutt'Ie (rom a comparison of the rcdox potemi.rl in artcri:d .nd c.u.m:uy vrrn hhwkl. F_flrrrts should I.e made to acc.+nqplish this R.ral by nurre srccncc :rntl relincd bicxlxrnical nuans Incurf~.rati..n .d lahrkd prccurarrs inltr suhtlratcs pres- ent in coronary vein bicxrtl may prove a useful subjrct for sludy. "CA'Ifi('llOt AMINE ('UNIIiNT OF: VARIOFIS OR(iANS IN F.X- PF:RIMI:NTA1. IIYF'FRl1iNSIUN." Ny A. Wegrnann, K. Kako, and R 1. Hing, Wayne State University ('r.llege of Medicine, 1)etrnit, Mich .Irnrrhan Journal of PhynrrhrRy. Vol. 2(/3, pages 61)7-6t)R, (ktoher 1962 Nephrogenic hypertensk.n in tk.Rs produced by a unilaterally placed Goidhl,lt clamp resullcd in diminished catcchr.lamine content in the thoracic aorta, bcoth kidneys, and in the spleen The catechrlamine content in heart, brain, and the adrenal glands rem:uncd unaffected. Neurogcnic hyfvcrtensitm, induced hy partial scction of butlcr ncrves, had no significanl ellrct on the calechtdamine content o( these organs. "1OFAl.('Al F ANI) MIIS('1T NU-IRITIVFi UI ())F) 1=I OW UIIRIN(i T(11)A(Y'O SMOKIN(i." Ily lay 1) ('trllman, M I) and Slanlcy l. lavetl, M I) , MatsachuseUs Mcrmorial Il-pit:ds, Flnst.rn, Mass 1'.nc rrdmRr .d the New FnRtnml ( nrdiuva.rtrldr SrrN rrrv. VrAuntc 7/1, p:rKrs 17 1Y. 1961 62. ( 1 I R (' rrantrc ( .r(Finan ) 1 hrrr it c ntlu tinP rvirirnre in Ihr few thr lrr. :rv:ulaF,lr in the ntrnccal litrr:.turr c-mrtninp rhe ctlcct rrf tuh:rrco .+n"kiny trn tkrlctal mntclc F.IrxKl 2 P I r1t.w In the use of a plethysmograph alone it is apparent that incrcaus in muscle hhMxd flow may be hidden or exaggerated by changes in skrn hhoxl flow; therefore, the ellect of lobaccu smoking has been investigaled by using the disappcarance rate of a radioactivy isolc+pe from skeletal muscle as . measure of its nutritive blood flow. VL Of a group of 17 normal subjecti, 14 showed an increase in skeletal muscle nutritive hlrxid flow as measured by the radioisotope disappearance rate during ciRarelte smoking despite small changes in total calf bl.Kxi flow and the usual decreases in skin temperature. The mechanism of such an increase is the unanswered question. These studies reveal its constancy and suggcst that inhalation is not a factor. Evidently an intact nervous supply to the limb is not nccessary since 3 of the 4 sympathectomized limbs showed an increased disappearance rale during smoking. Assuming that skin blood flow does not decreasc in sym- pathectam'ved limbs during cigarette amokins, it is not a simple shift uf bkxxl from the vasocanstricled skin to the muscle. "NORMAL ROENTGF:N(X;RAPIIIC ANATOMY OF 1111- IIUMAN C'IR('I.E OI' WILI.IS." By Robert A. Kuhn, M U., Department rif Neuro• surRery, New York Medical C'olkge, New York (ity. ./rnrruan l.nnnaf of R.wnrarnnloRy, Radium Therapy ond Nuclear Medicine, Vol R6, pages 104Q-IU49, tkcembcr 1961. In preparation for a study of the eRects of tobacco nn the cerchral cir- culation, it was important to establish the normal vascular pattern uf the human circle of Willis, the structural disRibutinn system which nsunilurs regional cerebral arterial flow. Fkvelopmenl of a technique of retrograde cercbral angiography to determine the flow patterns was accumpiishcd, in- volving the use of a 50% hypaque sotution. The technique avoids compietely pressure artifacts and arterial injury. ('kar visualization is obtained of components of the circle of Willis hy this nrelhrxl. The ohxrvalions indicate that the human circle of Willis is functionally a circk in name only. 1be independent an(erirtt and(x nterinr segments possess majcx differences in function. Internal camtid f1r~w nr.r- mally supplies the ipsilateral half of the anterior segmenl, hut abunl 50% of individuals possess the capacity to maintain adequate functiunal circu- lat<xy flow to Ivah hemispheres of the brain by way of one internal carr>tid artery. Posterior scgracnt circulation is characterized by bilateral dislrihuti.m of a relatively small volume of slowly moving arteriar hkxxl. I he brain strm and cerehellar areas are, therefure, usually isrdated frnm Ihc rapiJly nu.vin~ high volume stream{+crfusing the anterior segment. In the usual indivi.lual, if the inlernal caunid supply fails bilaterally there would appe:u to t.e little chance that hemispheral pcrfusion could be supported by way uf p.rsterurr segment ftow. "TIIE SPEFI) OF ('f?RFi11RA1. ('IR('l/l-ATlON " fly R.>hrrt A Kuhn, M[)., New Y.nk Mrdical ('rdleRe, I-h.wer and I-ifrh Avenuc (lr.tlrrr.rls, New Yrrrk ('ity Nrw l:nXl"nJ I~~ur+r.d (rf AfrJrrrnr, p:rl•rs .r,R-) r,v5 (ktobcr 4, 1962 2y

I In tirdcr to eslahlish a normal rate of cerebral hI(NMI circnlatinn. Icsfs were c't,ndutlcd in silt adults and one child by tetrngra(k hrachr:ll injectutn hcltlw Ihe riFht axdl>A and by high spced anRitrRraphy. Ihus hrttvttlrnR an n1,11,trlunity Inr stntly of brain circulatinn in a physruhgic sctlinK. 1 he average Ih(lraa-tn-th(xa: circulation lime in the at)nl1 is seven «c(,nds ( crchral Iransil usually is accclmplished within f(rur seconds 'f he vclncity (d hhNxl flow is quite high lhrou6h the antcrit+r segnient of the circlcs of Wdhs anA throuRh the nlerir,r and middk cerebral arlerics Flrain stem anti Iw,sleri(lr cerchtal irri6ation, by eonlrast, is sluggish Reasons f(rr these difiercnces are as yet unclear. Flow rates in children are more rapid than those in the adulf. Total cervical cerebral circuit may be complNed in as little as five or si seconds. Elemexlynamic facttxs are hclieved to he responsihk for Ihese higher veloci- ties 7}te capillary phax in adult and child is equal in duration - apprn><i- mately Iwu seconds. "n(-11ON 017 NI('OTINE: ON ('ORONARY VAS( I11 AR Rf SISI'- ANtT . IN INXiS" fly 1•hrytl F I catkrs and 1. P. I.a1R, Ikl,.rfthncnl of Pharmacnhrry, State l/niversily of Iowa Ctdkgc tit Medicinc, Iowa ('ity• Amrri(on Ir•urnaf rl/ l'hytir.LrRv. Vt,lnmr 2t)1, pages 62t-625, October 1962 (T I R('. grantee: 1~1nR) Nicotine adnrinistered intra brterially in adult mongrel dogs I,rtxluced an inctease in crntrnary va«ulnr r"r%lance as indicated by an rncrcase in cornnary fx•rlueit,n firessure It wa~ trrntluJctl thaf Ihia resulta Irnm synlpa- Ihctic ncrvrrua syvcm actuvily or rclc•.t.c• rrf tat(•thrd:lmincc Irnrll chrnnl:lllin tissuc (ian(ha fu f:rn/•htm hkr trru tnn't nrr .rl,l•.trcntlv invrrt.ttl Ihccnnlri(lrlt-frrrtr,•/lh• nrt- rr.Ir•rrn .r.tr-r•m.rry larln.urn prcccurt', W:" /nuca•t,1 ..rth rntr rrrl r.lnrrnr.tr.ru •n ,rl rur,rtrnc ur nurchincphrinc %mi synrl•.lthr trr nrr. .r..,r• I rrr. n/•,r, n rrl .e., ul.u ft•a.t ance was int'rca.cd by tht'tc .r)•rnt. .nrJ Irr• r Jurr . I lhr% nu I1 lu rnay trr nlay nnl I,c rclatcd ln Ihc rr+.ttrvt• rmrtrr•(a. ttir r ta td mt•rlrn(• lhc ullinlatc cRect tit mrr.l :utrrl.ll .ulnlnlr.trounn ti1 nrtrltrnct ctmlrary pl nunc (+revir,us rcPtals, is an rncrc.r.e in tnrrmary rt'~isl:mcc whith wtruld W exl.cctcJ ttl rc.ult in a dccrca.c in coronary hhroJ fluw. \ f oth.r Rrnnt(.r: (l S. Public Ileahh St•rvice. v11F Ifl)IiR(ifR SYNI)ROMli IN I11f: OR11_Nl." fly Vi.tt,r A Mc- Kusick and Will.nrf S Ilarric, I)cP:lrlrncnl tit McJicinc. Ihc lr,hn. Ihtflkins 1lnivcraity St'hrti,l ul Mcdicine :mrl Ih,.pital Ruffrlin of thr /trhrtt llrqr(ulf llucrunl, Vol 1011. paRes 241 1'/l, Ikcemhcr 1961 ( 1.1 R(' Rrantce: McKuci(k ) I I he IluctFcr syndrnntc oc't Iuaivc pcriphcr:rl va.cular tliwasc with Ihc cllnit:rl I,nturr dcacrthcd hy 11ucrIrt•r --- cxt'urs in rtlativcly hirh (re- qut•ncv in l:rlmn :,nd K(rrca :rnti Ilrr,h.,hty in nthcr f,:ut% rri the Oricnt. It alfctfa ilrnua cRCln.ivcly Ornet ts Itefnrc iS years nf a)•e in a rrlajt,rity td caa. Ihr ll.rntnts arc :dmrnt wuhnul cittcltlntn snntkcrc• u.ually hcavy .rnnkrr. I111• Ir1,1N'f elttt•Ill/tlC4 at wt•II tlt IIlC InW rRtrrrlltt/C\ ltc aflcrlcJ in a vl•nrlu:,n1 lur.l,r,rlrnn r,l t'or.r. Illrnnrhr,l,hkl•rtrt rKCUrc in •.r,mr c:rtrs nu,~ rrit rln•n w•rs Inund n•.rlnl rn rtlrntrlvrnl', tit nf Il.t' [lr:lc ml I• n.•n.rl .rrtr rr a A Irr rlur nt .rlfrrnryrnldnt IinJnl(• irl c:lt(•. wrlh lnwdve- I r I rurul td thc :unls was fK•rsislcnt'c :uld dil:datitru trl fh( nlfrru.,crrus artery wrth trclu.rnm ld Ihc radr:A :rnd or ulnar ar/ctics /Icc:tvtnl,rlly a ptd.c in a LcrhnatirlF hranth td thc inlcrns.cuus artery w:rs p:Jl..thlc :rt the wrist anrt cnrumunir:rur,ns Irttm the intcrn-,scuns arlery tn Ilrc uln:tr aftcry were itlen liftcd aflcritrF,raPhically in sumc In Ihc kFs a crrmparahlc fintling a fx•r- furalinR hranch td thc pcrrrneal atfcry communicalinK with Ihc fcrrnininal pnrlitrn (,f Ihc anlcri(,r tihial artery --- was uhscrveJ '1 hc dccl+ (c-ulural artery was tKclutlcd in Iwn patients. 2 In Krnca and lapan Ihe FloerXcr syndrume ex'cura Prethtminantly in farrncrs ar)d lalxlrcrs, pcrs(rns of luwesl .cK'i(x'ccsn(smic sl:rtn,% 3. (hhcr imPreasive epitkminhtRic fcalnres are expusurc antl tr:.urna, jend a low level tit dictary fat and prutcin consumption. 4 In a scrics tlf Iwenly-tigh1 cases two palicnis had ra-clusive Jiu:ne in Ihe diac artcriea with symplnms slarlinR at Ihe age of t) and 15 years :cnd pr,ssil.ly scttmrlary tit lrauma lu the hlw back and sacrum *1hcsc two taxs nlq+car It/ rcrfcacnt a cnndHitrn th.,tirxl Efom Ihe olhers. S. (n a majtrrity rr! Ihc cnscs :Nhcroscknnis, enchcdrsm unJ uc'cIusivc discase in the atata anti iliacs canntN :rccarnt for the pnrees. M(la t/l the cascc audred :rpl.car lu represenl a dislinct eateg(uy tit vascular drecasc which can legitimalcly he tcrmed ItucrRer's disease. 6. 1listotialhttlnRic study of amputated spccimcns Irrrm nine Knrcan males rcve:rlcJ n(+nspecific orFanizinR and recanalizin~, rK•clusitln 1n eight. In one chanFcs cttnsistcnl with suhacule angiilis were uli.crvcd. 7. Multil,le factors in the scries r/f cascs and perhaps even in imlivitlnal X ca.c may he reslw,nsihle lur fhe initiation and perpetuati(ln tit a pnK'css w ch is, within limifs, prnRressive. (Ab.r Rrnnrnrs: l/ S. Publie Ilcallh Servit'e and the Fi7ai Cttmp:tny, lapan "I llli UIII RG1-.R SYNI)ROMli IN l IIF. UNITIiI) SI A'1 IiS AR I VRII) IiRAI'IIII' (t11SI RVAIIONS, WIIII SPF('IA1- RI: .1+RI:N(T . 'll) INV(II VfMI'NI tlF *II11: III'1'F •R IiX•IRfiM1711?S ANI) 1111:. 1)1F- I I.RIiN I ln I ION I ROM A'1/11 RUti('I.E:RUSIS ANI) F•MIIUI ISM " Ily Vit'/ur MtKusick, Wi11:rrJ S Ilarris, OIc F Ullcccn cutd Rich:uJ M. (:(ntt)rnan, Ikp:ltlmt'nls tit Medicine and Radit,h+gy, 'Iht luhns Ilupkins (/niversily Srhtmd td Mcdreine and Ilospilal, Itallirnnrc. Md llullrrr+r ril r!v /(din.r llnplint ll.rcpunl. Vulume 110. paRes 14S•176, March 1962 Aurtngr•lnls, fcnloral arleri(,Rrams, and hrachial artcrirlrrarus were pcrGnrncd in twclve male patients wilh elinically typical Hn(•r(;cr syntlrt,rac lhc avcr:l)•c :ly.c tit nntct was 27 yt-ars (ranpe IS In 41) All wcrn cravy srnr,kt•rc, and ut mtrsl, remissi(m and relapce wcrr intim:rlely rcLucJ ar tcs satutn and re.unqNinn tit smrtkinR Sula'rfici:d I,hlel•itis had (rccurred in at (east frve (Nu. I, S, (l. 7 and 1//); hr.tt,luttrc matcnal suPprrrtinR the diayn(,.is uf Ilucrycr'. rh.c:r.c w,ri f,re.cnl in at Ir a.l tr,ur 1 Nt,. I, 1, 4:tnt1 G) :rnA will I,c tlra rrF,rrl rt.c•t.hwrr Nu rviJrntr r,r rw r In.rvc tttvca.r nl Itlr anrla anrf ir. I:u/•r I,r.rnt hr. .nl.tdy inlj Illt• rulrrtnrfrt•• w.r% dr.ttrvt'rcd Nr, cviJrntc tit :dhcrtr.tlrrt,.r. r,l nntr wnrthy prt.l>,nurmt w.lc d,.ctwt•rctl ll

I I)Ih rr Rrnnror: Natit+nal In,tilulcs of Ilcallh "(Y)RONARY VASOM(/lOR 1ONI1S Ifl A1111iROS( I FR(YIIC. tX1(iS." Ily ('ct•il F. ('rots and Rnlx•rt W. Ohlath, rkpartrncnl of Medical Rescatch, St. Jn.cph Ilnspilal, Ilurhank, ('alifurnia. .Imrrirun Irrurnal of 1'hvrir.h+RV. Vt+lume 2(12, paRcs h16-61R, April 1962. (1 I R('. Rrantee: 1)r. 1'. F. Salishury, t+f St. Jnul+h Ilnspital) An athcruKcnic reRirnen irnf+r+ud ufwn dt+gs resuttctl in several distinct ahnurrnalilies of Ihe ct+rt+nary circulalitm. 'Ihe stnrclure nf the curt+nary arlcrics was mcxlificd by cx/cnsivc aIltcnnnalous dcpt+t6ts 1)urinR cnntrul states before the adrninistratitm of v,sr+activc drugs, ct+rr+n;try Iluw Lcr unit of nel Prnpuldve force and heart wcirht was unvaricd or int rr;rxd '1 he ahility of Ilrc athrromahrus cortrn:rry Irce tu inctcase of dt'crl•:1sc its vuso- nttNtrr hmus was ntarkcdly tur/ai1cJ Ihc ahnt+rm;Jitics rcturJcJ wcrc pro- )a+rlit+nal with Ihc sevrrity uf the athcrrrFcnic rc'imcn. "I (K'AI.( IR(lI1.AllON IN I11 ARf MtIS('I 1:. Slltl)Ilil) WI111 Na" ('I I ARAN( 1 M1: 111O11" Hy 1'ttcr F. S:+li.hury, ('ccrl 1? ('rr+«. Rr+hcrt W( )hl.,lh. .ir+d I' Amlrr Rithrn, I lt l+:rrtn+t nl .•1 /\1rJrt .+l It,•ar.+rt h, CI 1„a.l,li 11 J J.•I It-r•LrJ 1'h ...•L•vv •',d I . • J„I,.ii, I r 7he disappcarance of radioactivity of Na7'('1, injected inlu the left ventricular walls of dugs, was studied. 'Ihc tate of Na t'Icaran(c /nnn indi- virlual dcpr+sits did not measure coronary flow in abw+lutc tertns; ht+wevcr, whcn, in the cuurse of a single ubservation, the Na clcarance sh+pc thanRed, this reflccted directional variatirxrs of coronary flow Incrcascd central coronary pressure did not always result in more rapid s.xlium clcarance from ischemic regions of heart nmusck. "Vr?N1R1('(JLAR PERFORMAN('E MODIFIED HY fa-AS1I(' 1'R(1P- I:R71[:S OF Otl ff•l-OW SY$I I:M." 8y Peter F. Salisbury, M I) , I'h 1) , Cecil F. C'russ, and P. Andre Riehen, B.S. Circulation Rrsrarr-h, Vulume 11. pages )19-32R, August 1962. Left venlricular and alrial pressures, left venlricular circunrfcrcncc and hroqitudinal meridinnal seRnxnt kngth, coronary nuw, antl cardiac t+syRcn cnnsumptian were measured in dogs under conditiuns which pcnmltrd in- 1enliunal variatitm nf the elasticity of the k/t vcnlricular uu111nw sy.trm, and cnmparisnn ul cardrac f.erfrumance dutinR slcady statcs in which hr;ut rale, tlrnke wtak, and strrrke vulunx were itkntucal hut the uutOt+w rla.lic aurale capacity was varied. When cje.tinR through a rigid syslem, the left venlricle grncratcd a larger fraclit+n (If the conlraetik lensirus under au,cott+nic t•nnthlitrrts, and kfl ventticular systolic pcak pressure was increased Ijecliuns tlutmRh riRiJ lul.cs pruc•ceded f'.om higher diastnlic ventricular prc.sures and largcr cx- tcrnal din.cnsitms. 'Ihe eRrxt t+f hearts whit•h ejected thruugh ril;id uutlh+w eur uits was aca+ciated with a reduced myocardi;J oRygcn crrnsumption. rb.r Rrnntnr: National Ileart Instilule. "CORONARY VASOMOTOR TONl/S IN MOI)f.RA'11: . IIYPO- '1111?RMIA." Ily Cecil E. ('ross, P. Andre Riehen and Peter 1-. Salishury, /(parhncnt of Medical Research, SI Joseph Flaspital, Ihrrhank. ('alifornia. Arnrrit'an luurnal.rl PhysinlnRy. Volume 201. pages 825 N2R, Nuvcrnhrr 1962. ('txona+y hhxxl flow was measured in mongrel daRs in open chest preparalNros with fiaed cardiac output and by-passed riRht heart Arterial oxyRen tensiun (/)Or), pll, and tempcrature were measureJ. 'Ihe slope of felfressnMl lines between mean coronary driving pressure (anrtic pressure minus Icf1 vcntricular pressure) and coronary flow indicated dircctitrnal changes of cnrnnary vasomntor k.nus. 1lurinR ~critx)s of bkxxl cooling in non-failing hcarts, the ctannary vessels dil;tlcd only when pOt was perntitled la rall, hul not when it renr.rincd s/ahle. In failing hearts, mrxkrate decreases of tbhMUl 1cnq+crumc and f+l tr t1iJ not cause hnthcr decrenxnls of coronary va,r+moh+r Innus 1 hc J.rl:+ indicalc that diminishrd pUr and nrN h+w hlrMKl terulr•ralruc wac dre tattta Ih;tl cautcd ctntmary vastklilalitm. I V. 1'a-rch.rl'h v.iuloRiral Srudi.'. "( ()NCIIIItIIUN ANI) SMt/KIN(i IN IIAI IAN AMI Itl( nN I A(' I(/ItY W(IItKI ItS" Ilv AllK- rl 1).rnu+n. Ik•I+.ulnu•m of I It+Jrnuwhq'v, I/:+tv;utl Stlr+,t•I of 1'nhhr 11cal,h, 11,104.11. M:+.a .Inrrrr„nr lorrrrr.d of 11 I hcac anrl nthcr artcrnt+pral+hic fmdings wcre strikinl;ly similar to thnse in Kracan pitrrnla with Iht• ItucrKrr syndrtauc (MtKu.itk cl iI , I(rdl. Irrlrns I1or6rrrr llrr+l+ I//v.2J2, I')(+1 ) It is ct+ntludcd thal the artcrit-Rr.+l+hic fcalures are sulficicnlly charac/rristic to rc)+rrscnl one mnrc argurncnl that IlucrRcr's diac;tse eltis/s as an entity dittincl frran nther hrnns ul artcrial discate Whelhcr thc disordcr is of esscnti;Jly inllanunak+ry narure• that is, a,rt anRiitit• is a prtthlem fot histologic study. `/C)thrr Rrnnror: National Inslitutes of lleallh "RUr?R(;FR'S 1)ISEASE: A bISTINCT ('I.INI('A1. ANI) PA1lIO- I.(XiI(' IiN 111Y " fly Victor A. McKusick, M f).. Willard S Ilartis, M 1) , (1k [i. Ottescn, M f)., Richard M Gtrodman• M.1)., William M Shclley, M f)., and Rnhc•rt 1). Ohwxlwell, M I)., Johns llupkins Flosriital and Schood (if Medicinc, (laltimnre, Md. Inurnal n( drr .Imrriran MrJital .lstrxiarirrn, paRcs 5 t2• July 7, 1962. Artcrir,Rral+hic and hislt+patht+h+Ric sludy of a Rnur `+ nf 22 f+alicnts in 1lahimurc pruvitkd cvukncc Ihat IlucrRcr's dnr.rsc is a rlistint•t rl6tical and Ir:t,huh+rit cntuv /'rectK•it.us athcrtnckrrnis antl sysleruic cr11tN1I15m can be extlurIctl na dLt••nt+.tic fx+%sihililit•s Althtrugh thc chanpes in Ihc vcsaels are infl:unm:utuy in ch;uactrr, it m:ry nul ft+lh+w that Iht•) rer,rrsrnt an anRiilis cr'nil+ar;rhk Itr pcurarrcrrtis 'I~c ctiuh+gic faclnrs and the pathr+Renclic nrcchanivna in Ilucrgcr's Jiaca c are ill undcrstrhal In Ktrrca ind Jal+.ur. whrrc llucrRrr's diarise is rcLuivcly mnrc cnm- mt+n than in the IlnitcJ Statca, cRPoaure tr+tiarnl+ctrhl and Luttihl)• lu Irauma secmcd Ir+Lc inni:rung f:ult+ra in thc yrronR ni:+lc suhjccls slutlrcJ, nearly all nf wht+m Irvt•d in (arrn vrllal•cs +nJ w,qkctl h+ng huurs in ricc l+atldit•s with h;u+ds and fcct in crd,l w:tl,•r In htuh Ihc oriental antl Italtunr+rc cases, ciRarcllr --mr+kmK %ct-rncd tn hc a I++rtcnl cxaccrb:dinR and IxrIK•tualing fac- t rn the Jiteatir

I 1'hc(ir ul .4nrlrrrrlnrlnxv, V(rlumc 20. pifct 67 04. M:rrtIr I'7Q Uct aucc of incuncialcnl an(I infr(vlucnt ref.ratc (d :lccoci.rurm rd trrh:rcc(+ snrrrking with h(Kly f(rrrt+, it was rlcti(ahlc trr tturly :rc hrmrrr)~( ucrwt a gruup hirrh+Rically and cuhurally - as p(+ctihlc, in this cacc 167 a(lutl ru:(Itt, agcrl 2// t'), wr+rkinR in :r cinglt• f:rchrry, all with ra(t•ntc hrrrn wrlhin 75 rnirrt of N:rldcc, Itnly; 151 were h(+rn in the I lnitcd Sl:rlcc ISrnrrnR ncccc 167 rnen, Ihc kaner inr6viJnalc smrrk(-d ti)tnrficantly nnnc than Ihc cl(rut .rr fat ( hul not muscular ) ont•s, I hc hrccrial (rK llicicnls rrf crrrrrl:)trr.n were hiFher when n(m-smnkcrc and lighl s(nnkcrc werc crmr- h:rrc(1 with heivy srn(rkcrs than when non smrrkcrc were crMnparcd with a11 snlr,kcrs. lhe attOc/alMns, thtwrgh siRnif(cant, were nnl p:rrticularly chrce, (rr cl(xc cntargh for individual prcdiclion, with hiarial crwllicicntc front O 25 to O )5. lhe asarciati(m was not due to different diels, since amnking and calrrric intake. total as well as percentages nf protein, carh.rhydr:He, t(rtal fat, saluratt•d fatly acids, and p(dyunsaturatcd fatry aci(Is were n(rt asan-, cialyd As rcf.rrrted by previous aulh(wt, terum chrrlcstcnd tcvclt wcrc aig- nificnntly hiRher in Ihe smolers ('(mlrary In /rrcvinus rclwrrrs, the sm(rken were nit Icse maaculine in phytiquc, nrr m(rre atlive, anJ c(nnumed no mnrre alcrrh(d Ilun nan-smokers. "SIUf)IFS ON FIC7URf: hRA1VINGS: A RfiVII:W OF 7IIE 1.11 FRA- 1 ( IR1: ( 1949 I959)." Dy I c(ma W Juncs, I'h 1) , and (':vnline il 1 h(nnas, M I) Jrrhnt Ilrqskina Ilnivcrcity Sch(wd (+f Mcdicinc, Ilaltnnrrrc, M:uyland. 7 hr l'(y( hrntr,( ()unrrrrlv S(qrrlrrnrnf V(rl 15, p;rKct 212 261 , 1'art 11, 1961 (1 I R (' Rrinlee' '1hrrm:(t) As part (,f a long lerrn study of prccurarat of hyl.crlentirm and coronary arlcry discatc, r(gure drawinRt have Lccn rrhlaincd from 179 Juhns 1lrrpkins nxdical stutknts in twelve cuicective clatccs 'Ihe present paper is a review nf the fiFure drawing literat(rre f(rr the purlxrtc of pruvirlinR a hrnad hack- grtrund f(rr a pnrjecled scrics of elurlict in regard to figure (IrawinRt, their scoring clietification and Iheir use in the apprais:il of personality in a poru- latinn (if healthy mcdical stu(knts on whom a wide variety of (rthcr data are available. In ftrrlhc(+ming arlicles, c(rmraris(rnt will be made fw Iwern the fiRure drawingc of sm(rkcrs and noncm(,kers, of suhjccts with arrd wilh(rut a parental hitl(ay of hypertcnsi(rn and, or c(rronary artery diccasc, with and without hyf.crch(rlcsleremi3, and with diflcrenl types nf f>,rly build l he review of the fiRute drawinR literature from (949 thnouKh 1959 indicates an enrandinR interest in the use of the I)raw-A-f'e(s(rn tecl, with increatinR emphacis on ils role ae a research 1(xrl. As yet there is no uni- versally accepted scoring prrK-edure, allhnuf'h ruany inveuiK:(Irrrs have de- viced their own sc(Hing elactificali(rn At least un( tnaj(a audy has dcm- onttratcrl that reliable scoring can Lc achieved and that st:rliclically signifi- einl t'(rrrt•lati(ms can he marlc ht•Iwcen acrucs fur ArawinKs and (rlhcr tctls scrrret such as Ih(rse of the Rortch:uh test Accrrrd(ngly, Ihe aulhras fKlieve thal Ihe 1)r:lw A 1'ersrrn Iest ntlcra Inrmric(• rrf tk•tr+minR a trnrldc, elfective, ptythrrlr,Ric.rl tcrr(•ninR tkvirc frrr Ihc fcrarm:rlilv al ~r.ri~:d nl nrrrnral suF,jrr tt "( ()41f'AklY)N l11 `M(lKl 1tC ANI/ N(1N %n/t/Kl 14% It 1111 111S I 'IR111111IIIN (11: A11O :(n(I Rh(1)1 Ill(N)I) (:ROtll'S•' Ily Ilcrnrte llir« hhrnn ('rnct-n, Johns Ilripkint lJniversily Sch(arl (d I lypicnc :rnd 1'uF+tic IIt-.(lltr, :mrl (':rr(rl(nt' Itcdcll Ihumac, Johns Ilnl+kinc Ilnivcr.ily tichrwd (uf Mr•rli( ine, nnltinunc, Md Jhdrrrnr.,f thr /(rhnt Nrrpl int 17,•(lr,rul. Vol 1 lo, pagcs 1 7. January 1962. 'Ihc rlittrihuriuns of the A11O and Rh hl(xwl gr(rups amrrng 11'IR hcallhy white and Negro men classified acc(+rding tu smtrkinR h:dr(Is wcre cx:(nrined Nn siRnific:(nt differences wete faund in any of Iltc c(rmparit(rns among the Ncgrn malcs. Am(•ng the while maks. there appeared to be a siFnificant deficiency of group 1; individuals anronR heavy cigarette smnkcrs and an excess of group 11 persrnrs among non-smakers anti urcasitmal smukcrs. Art excess of Rh negative in(livi(Inals was f(wnd in the while rt- easiun:J smoker group. The diRerences, while not markc(1. seem sutlicienl lu rranl furlhcr investigation of the relationchip between trnokinR habits d M(KKl groups, along with other pertinenl variables. Orh.r Rrnnr..r: I1 S Puhlic Ilealth Service. "1`SYC'lh)1 (N;I('A1., ('111:I1/RAt., ANl) IIf:AI:lll ('l1ARA('11•R- IS11('S (H' AGIN(: SMOKI:RS ANI) NUN-SM(1KFRS " Ily Fredc(ic1 Ii Whickin, M 1)., Andrew S bihner, 1'h [)., and Paul l. Rhudick, 1'h 1) , the Age ('enler of New FinRland, Inc.. Boslexr, Mass. 1(nnnuf n/ l:rrr,nadrrxy. Volume 17, pages 69-74. January 1962. ('1' I.R C. Rrantce Whiskin) IkmuRraphic, hcallh, psychological, and cultural charactcrislics of 4112 aged smukers and nnn-snNlkcrs were examined with yuetti(mnaire lech- niyucs.'Ihcre were IS) n,ak smokers and 21 mak non cmukcrs and 126 fc(nale smnkers and 1(12 femak n(m-smokers in the study gruup. None of thc hypotheses pertaining to health auitudcs of psychological factors was suhstantiated. Flowever, such dimensions as inh:(ling and allitude Inward srnnkinR among smokers seemed to be related to ccrtain pcycho- I(rgica) and health attitudes as well as to onset and cessali(m of the hahil. M(rst men started smoking before they were 25 ye:us (rld (R(/')f.), while only J5"• nf the wnmcn had started bv Ihcn; 42^/., tif Ihc n(rn hut (Mtly 29% (rf the women refxuted they inhakd; 52% of the mcn anrl 17^:, of tlre wumcn fclt r»(r(e relaxed while srnukinr; It/'"r of the nrcn and h':• of the wurnen fell sm(rkinR was beneficial, anti 29% of llre men n((tl 22"S• of Ihe wunrcn thought it harmful. Only ahrn(1 1S^h% of each Rrnup said they wanted t(r cut drrwn their snrokrng, and only une-Ihird of the men and wnmen believed Ihcy cunld dcfinilely sh+p snrnking if they so desircd. 'I he dimenei(rna of inhaling vctsus n(ro-inhaling Ien(Icd Irr tcp:rralc 1lhe sm(rkcrs intrr two grouhs which ah(rwed stalistically tiFnitic:ml d(Ilerences with resl•t•cl lu Ihc fulhrwinR chara( Icrislics: inhalcrc arc rnurc cun( erncd ahrrul tfu•ir h(•ulth, Icnrl In f.elieve /h:H tmrrking is h:rrurful 141 tlu•m, arc nrrl rcrt:rin thal thry cnuld atrhi arnnkinK, fccl kss rcLaac(1 whrlc cmrrlrny, cnurkc mnrc nrrn fillrr ciKarrllcc, snaoke nnne of Ihe cigarclle, and tl.utcd anwkinK at :rn canccr cnnyr:ucd 1(r the n(rn iohalcrs. Arurrnp trnrrArr..rnd nrrn cmnkrrc, cultur:O fart(vs w('rc of rmlrn(tanrr, Ir.ulirr(I:rrly rn rr latirrn trr Ir:ucr(r:rl :rtlinrdc Snurkt-rs rrr I-Ih .cact herl fathcr% who trurrkc rrn rc rdlcn 111.111 w:rc 111t' c:rte with nrrn cnrnAr rt Wurnrn r1, "' 1S rn 1 ,T~

I I smtrkcrs morc frequently indicated parental rrstriclions on srnrrking than wr,mcn who did not snrr.ke. An interrtting ohservalion whic'h is diflicult to evaluare it that mnst smtrkcrs diJ not rersrtl being influcnced by the puhlicizcJ f~ndings tff a re- latinnship luween smoking and cancer. lhis atlitode cecrns cuntradictory, hccautc sutne uf the retpontlents who so IcslxintkJ nonetheless admitted cnncern over health. 1 hic flndins may he influcncrJ by the fact that nlJer {xrrpk in general exhibit more health concern but do not nccestarily relate it to their smoking habits. V. Tobacco Cherr><eetry ond 1li.>,c.hpmestry "AFFINITY OF PC)LYCYCI.IC AROMATIC F1YDR(X'ARAONS FOR EI.F('TRONS WITFI TfIERMAI, F.NERGIFS: ITS POSSIRI.F? SIGNIF- I('AN('li IN ('AR('IN(K;FiNESIS." By Prof. 1. E. I oveltxk, National Institute for Medical Researrh, Lrmtlon, EinRland, and Prof A'l.latkis and /'rof. R S Ilecker, Fhpartment of ('hemislry, llniversity u( I luuston, l eaas. Norurr. February 10, 1962, paRes 540-541. (T.I.R.C. grantee: Recker) In pr_wious studies (l.tsvelt.ck, 19(>n-61) it was ftamJ that most classef of hydrocarbon had little or no alTinity for free electrons with thermal enerRics, allhouRh there were several eaceptions to this rule. 't his paper refxxts some preliminary ohscrvaliune on the ekctrtxs allinity of tctracyclic aromatic hydrocarhons, nnd ditcutces tlrc poscihle reLdionthip hetween electron aflinily and carcinogenic aclivi/y with these and other ct+mpounds. The retnlts of the e><prrirucnlt indicate Ih:rt amrma hydmcartwnt the class of prrlycyclic aromatic curuprundt is unusual in fxrtccsting mnny mcm- hcrs Mith a high affinily Atr /rrc clcctrnns. So far, mtrst etpcrimcntal work and theoretical conciderations of the ekctnrn Jictrihulitm in thrte cum- Iwrunds has been enncerned with their unJtruhted ability tu /unclitrn ns elcctron dunurs 'This fact has tentkd to overshadow the estenually amhiva- Icnt character of the polycyclic hydnrcartxms in eleclron transfer reactions. It was suggested in the previous studies that the tur<icity or biological activity of cornPt„mJs with high elcclron affinity was altrihutahlc Irt their ability tn fnrxtitm s ineversihk electron traPs, so that the nnrmal /ran+frr of elcctront during tr><idative phrnphnrylation was inrPaircJ "I he Ltrscihility that carcinrrgcnic activity is a special ctampk of this furm of toRic atiirrn is suPfarrteJ by recenl eRfxrirnenlal wtnk (Aflison, A. C. & 1.ighttrrwn, 1. W., Nrurnr IRV N92, 1961 ) 'Ihe notion that an ahi!ily tn extract elcctrons fnmr Ihcir n:rrm:tl path in a livinr, cell is a necctsary nnrkcul.rr fxrqicrly nf a major clats of chcmic•rl caninrrKent is tlxculative, but is relatively act-cssihte to earx rinrenl:tl veri- fication ur drnial. "I'O11NIIA1 MI?lllOI) I(1R 1111: 1)1.l1ItMINAl1ON O1 I:1I('- 'IFtON AI I INIIII SOF M/>I I( 1111 ti AI'I'l l('AI II/N Itl SOM1 Altltl\1A111 IIY111((H AIttl()Nti" Ily W I N't•nlwrnth .rnJ /t.rl/rh S Itr r Ar r, I h I.nlrncnt rr/ ( hcnu.lry, 1!nrvrttity r,l I lrntttun, I tt.rt /nurnrtl ul the .~rrr~rrr,rn 1!rirrrnnl C,nv/h'. urrl H./, trrt!c%4?h) •126h, Nnv 20.1962 1( I Onc of Ihc principal aims rd this invettiRatinn is Io ett.thlith Ihc fund.t ntental naturr of the clectmn capture by mtrleculea tK•crnrml; in a detrctnr used in ga, chnrmatoRraphy. '1he inlerPretalitrn is devclnlxd in Irrnn rd an eyuihhrium helwrcn the neutral mtrkcules and the electrons the rclatinn- ship between the cleclron capture crnllicit:nte and the ekctrrm allimlret of mrrlrculcc is ffrcecnlcJ. -Ihe teculls for a seriec of arofnatic hyJrocartNms arc ctrml.arcd to Ihc half wave reduction I+tdcntials. II certain atsumptitNts are nradr errnccrninR the ratio t>< the partition functions of the Raseous negative ion of Ihr mukcuk to the neutral Saeeuus mrdecuk. Iltc ckctrun allinily of the mnlccule can he estimated. 'Ihie has bern c:vricJ out fur anlhracene and other arumalie hydrocarl.ont, and the rrsults cumparc lav- orahly with recent Ihroretically calculated values. "STUf)IES ON 'f11E DIOSYNTHF:SIS OF TFIF: PYRII)INF RING Oh NI('()I'INF:." fly fht.mas (iriffilh, Kenneth P. Ilellman. and Richard tl. FFycrrum. Ikparlmenl of Ilinchenmistry, Michigan Stale l/nivetsily, Fast l.anvng HNxhrrniUrv, Vrdulne I, pages J)6-)R). March 1962 ( f I,R 1'. grantee: Hycrrum) Tuhacco planls were fed three ('"-IalxkJ suhstratec to autly their rule in the hitxynlhctis of the pyridine ring of nicotine. (7/yccrul. 2 ("1 %nJ atp:trlic aciJ-1-('t4 both contributed relatively large yuanlilicc uf radi~ractive eatFxm to the pyridine rinR, whereas Prupritsnate-l ('r• did not (7lyccrul- 2('" was innorptratcJ into the Pyridine ring It) about thc tarnr c>tlcnl as Rlycerol-I,1-( t, Partial Jcgradatiun nf the pyriJine rin of nicntine front Plants fcJ atp:utic aeid-l-('rr revealed that acPartic nciwas not ctrnverletl direclly Irr the ring. eince ('" was located in mtxe than a single I+tnilitrn irn the IinR lhe three IahclcJ compoundc all ctsnltihuleJ ('14 141 the pyrroliJine ring ul nic- ttline. 'I he patlcrn of labeling in the pyrroliJine ring suFRc.ICJ Ih:tt Pnqrri nnale was cunverteJ to acetate before utilization for nicotine hirnynthctic. "Sl'Nl1I1SIS UF PYRROI.11)INE RING OF NI('(YI'IN14 FROM SI: .V- FRAI. ('"-I.AIlF-1.1;I1 MEilAI4(1LIlIS OF NI('(11IANA RIIS11('r1." fly 1'ci-IlcinR l.in Wu, lhtsmac (:rillith, and Richard C ltycrtum, Kcrlrie ('hemical I.ahr+raa+ry. Michigan State University. Uast I ancinR /rnr.nol rr/ HirrlrrKirul ('hrnrirlry, Vnlumc 217, pages 8R7-R911, March I'7tr2. Acclate, pnprionale, glycerol, and ntpartalc were ulilifcJ hy Nrrnnnnn rrrrrir u frtr cynlhcsts of the pyrrnlidine ring of nicotine. Wht•n accl:tlc I(", was melrFNrlizcJ by Ihc planlc over a 7 Aay PCrnxl. 95^L• 411 Ihc ('" incurp rrralcJ irHtr nitrdhnc w:n IkrcateJ in Ihe Pyrru1rJure rinK and Ihre was cynally JivideJ betwecn positions 2 and 5. 'I he hrcation uf ('" in the pyrrulidine rinR aflci feeding I:rht led acrtalr, prnf,ri„nate. Rlycernl nr asp:utate for v:vyinR (reritxls u( tirnr w.tt ctm.iaenl with Ihe hypothesis lhat thece meratwditet were cunverlr.l f.y way rff Ply colytic and Ihr IfIcJ1Ixlityl/t- aCnl tytlr /o alPh:r krUrglut:u:rlc. Rhrl,rnt.tlr, anrl n tymrut•trical inrrrnrcrli:ttc, utlim.ucly lu yirld Ihe Iryrrnlrrlinr rmp, "Alll(1X11>All(1N l)1 NI('()fINl: 11 I'ROI)11(-I'S ANI) I'KOI'OS1 I) MI[('11ANISM." Ily V 1'.tul (;avin and RulK•rl II Lrnncll, 11(p:utnrr-nl nf 17

i ( hcnri.lry, Ilmvcrsily rrl Vcrmnnt. IlurlinRlrrn 7nhu,,.r .Grrrr,r, Vrd 6, p:+Rcs 29 11, f chruary 21, 1962 ( I I R('. Frantce I inncll) Nticr-tinc w:rs vacuum dradlcd md uxidncJ hy Or at 40' (C 'llic lit(41110% wcrc tludreJ hy p.rl.cr chnrm:rtnKraphy, infrarcd srrctrrrNcry+y and K:ul I rsher tilraUrrn for watcr. AMwI 2(IrX% of Ihe O, aplx•arerl .+c cnlinine and another 211^;. as n.ynicr+tine, whercas 30^,'n alq.carcd as wa/er An un- sl:rhle hydrrrl+crn.iJc i% pnrpncd lhis could split uu1 waler lu fonn crrtinine, II,Of tu furm an unslahle diradical (which could Ihcn reatl (urthcr tn frrrm hry-hcr mulct'ular wciRht lars and account for the h:d:rnce of the O+ cr.nwmf.- liun) and the 11,0. would thcn reacl with nicotinc Irr furm the rriyniculine. '1 hc rcactirrn is hclicvcd /'n prtrcced by a free tadic:rl rncchani.m "RliSlt)IIAI. ARSUNI(' IN SOIIS AND (Y)N( EiNIRATI(1N IN TO- RA('( () " Ily Ff G Small, Jr. and ('. R Mc('anls, I)CPartmcnt !+r Snil Science, Nnrlh (':vulina ARricultutal 1?.rcrimrnl Sl:rlir,n, NrNtlr ('arnlina Slate ( r+IlrRc, RalriRh I rrhur fn.Cr irnr r, Vrrtumc 6, paRet 14 36. Mar( h 9, 1962 ( I 1 R ( ' gr.ntec: Mc('anls) Ar%cnicals wcre rcmrwed in 1952 frnm the list u/ recrrnuucnded inscc- IiriJcs /rrr crrn(r,d of hornwnrrns nn Irdraccn and crrnsidcrahle rviJrnce inJrcalcs that aincc Ihat tirne there has l-ccn a sharp dccrcaae in Ihe artcnic cnntrnt of ciRaretres The nhjcctrOt•s of this study were to evaluale the ab• sorplurn of arwnic hy trrhacco and tu rrhlain an catim:rte of the residual Icvels of :rrscmc in rcilrescnlative crrilc ucrJ (nr Urhaccrr ptrKluctinn in Nrrrlh (-arulrna 1 hc survey includcd all u( Ilrc mij,a IrrhaccO pnntut'rnR arcas in Ihc slalc Arkrrnt in aul was fuuml lu r:rnl-r rrrrur I tu S hartt per nrilli,rn, wilh au avcrayc Irir all atcm rr/ 2 R I+.rrts L, r nulh,rn I hr% vnlac is ctusc tr+ the 4 I+,uth Ircr nnlhun rclxrrlcJ hy (irc.rvr., I I(/fr,r ( h,•m llr,ll 2 H SI'/ 521, 1911 ) for a virp,in a,il anJ thut suy)T.ts nu measur:rhlc incrcase lnnn the previous use rrf :uscnicalc in lhe arscnic crrntcnt of srrik uscrl I,n Irrh:rccu- 1he arsenic crrncentratiirn Irrund in the eurcdlc:rl in ad,:+rco Rtrrwn on lhcte trrils is lrom O 5 to 3 S Irarts per nrilhrrn '1 he averaRc (rv all leaf samplrs is I S rarls per million. "INI I.111 N('F. Of' ARSI?NI(' APf'I II:I) TO 111E (iROW 11l MI:I)IA ON '1111: . ARSFNIC ('ON11iN1' (/F FE.EIE? ('IIRI•h 1OhA('(-O" Ily 11 (7 Small, )r arrd C 11 Mc('ants Agrnnrnny Irrurnal, Volume 54, pages 129 111. March April 1962. I ielJ and Rrecnhouse e><petiments were cnnduc,ed to study the ah- sorp lir+n of arsenic by flue-cured tuh:rccu 'Ihe field Itealmrnls consicteJ of a chcck and ratrs rrf lead arscnatc 117'1~ At) cquivalcnl Iu 1. h, 12. 24 and 4K f,rrunds of arsrnic per atre alqphrJ lu three drlletcnl toil tyl+es in Ihe spring rrf 1957 I:afa•rimenls were crm,luctcd rrn the sarue sites in 19SR and 1459, hul nr, arldiU,rrt:d As wnt :y.lrlrrd In thc /rrcnhrruac. Irrh:tcru wM Rruwn in sanJ tvltures Irr whirh w.rt :rJrk•d a nulrirnt arrlulinn tordauunR varirrut cr+nccntralirrrrs uf snluldc As aa a awlium aracn.dc 11:r/.r fr,rm rhr Iirld ew1rcrinrcnta .hnw that Ihr rrrnrrnlratinn of artcnic m,hr turcrl t~.hntr„ v.urcd Irrun 2 l+.utt Ircr nullrun whcrc nrrnc w:n applied tn thr tr,il Irr :r m.r.rnranr rd I•1 1 I,.uts Ix•r rntllion :d the 4H L.runvl ratc. 1H l hcre was crrntider:rhlc varialirm in the arsenic content n/ Ihe Iravrs la twecn ycars and Ir.ratiuns In Renrral, the cueccnlratNxt was hiKhru rn thrnc Icavcs Rrr+wn in thc s,nJrcr sr+il and dccreased as tltc clay and iron crrolcnl rrl the sr+il increascd I'h,nphouus ferlilizatirxt also a0cclcd nce level of As in the Icaf. Ihe cr+ntcnt being hiKl,tr where fertilizcr phosphutus was apldird Ihan whcre i/ was nmitted. lhc arscnic content of the aerial porticm of plants grown in the grcen- hr+use was ccrntirkrahly lower than that of the n><rts, suggesting Ihal the Irrw Icvels of As in Icaves from field grown plants may be due in part to lhc I:rck of appreciable Iranslocation. "UI:MEilI1YL.AlION IN TE1E M[-:TAROI-ISM OF (-) NI('(YIINI'." Ily Elcnccrt McKennis, 1r ,(.ennnx R. 'furnbull, St+rcal E.. Schwartz, hino- suke Tamaki and Edward R. Bowman, Ek arltncnl of PharmaculoRy, Med- ical ('rdkRc (rf Virginia. Richntnnd, lrrarnoPirl Rioloxicaf ('hrnrirnv. Vnlume 217, paRes 541 546. February 1962. (T.I.R.C. grantee: McKcnnis) 1 he mctahuli,m o/ (-)-nicoline mcthyl-C" has been sludied in both Ihe ral and the duR Aller adrninis/ralitrn to the former sfxxcics, R/n 11% of 1he raJirr:rctivily of the dose appeared as respiratory cartNm din.ide An examination of the nxtabMdism of (-)-niculine and tho intcrmediale (-)- culinine in hc+lh species kads to the eonclusi+n that the ennversirrn of cotinine Iu Jcmcthykolinine is involved in the fornation of carlx+n dir+><ide-(C', After administralion of nierNine-melhyl-C", calininc and Ramn+a (1- pyridyl)-hcla-nxo-N melhylbulyramide wrre isolaled Irv+ut the ruine of drrRs. All of the radir,activity of the lalter compnund was fr+nnd in lite N mcthyl grrrup. As a crrnu•quence of Ihis and (Nlxr ennsiJeratirms, nrcthylamine arising frnm the hyJrr+lysis of the keto ami<k in rivrr rnay I+c an intcrtnediale in tlrc furtnaliun of Ihc carMm diotide-('" ubserved in the sluJics \/ Of/rer Rrnntnr: Ametican Tr+haeeo Company. "STE/hll'S ON 'lllE ME'fARUI-ISM OF (-) CO11NIN1: IN Tltf 1111MAN " Ry 1?dward R. Elowman and Ilerherl MrKcm+is, Jr , t)cparlrncnt nf PharmacoluKy, Medical ('olkge of Virginia. Richrnrrurl Inurn,rf n/ 1'Ir.nrrrrncnGrgy rnrd Fr/rrrimrnlof 1 hrrnprurn s. Vol. 135. pahrs lllh 111. March 1962. After the inRestiun of (-)-entinine. Ihc male hurnan cxctrlcd O- enlinine, hydrn.ycntinine, and Ramma-(1 pyridyll-heta t+cn N methylhutyr- amide. lhe Iwn rnetaMdiles were isolaled frotn urine s•rrd idcnUlic•J hy cum- patison with aulhcntic comprrunds prevk>usly isolated /rum drrR ruu+c 'Ihe data indicate areas of sirnilarily hetween the duR and Ihc humrn in Ihc mclahrdivn n/ ( crNinine. In conlratt, huwcvcr, nc+ dcsmclhylnNininc was frrund in Ihr unnc of Iwo human subjects afler oral adminitlr:rUrrn rrl () eulinine. I/ydrnRycnrinine has heen r.l.lained for Ihe firal timr rn rrytt.rlhne ftNm "SI11111FS ON 'I111: RI:SI'IRATORY ANI) ('ARhIOVASI Itl AR f:l - /-T("ISOF I ) ('/)IININI?."Ilylnuphl: Ilnrzrlleca,I:dw:ur/I( Il,rwm.rn anJ Ilcrhrrt MtKrnnis, lr , 1)cf+artuncnl of Ph.rrnacrdr,Ry, Mrvht:J ('rrllrPr of Virginia. RichmrmJ luurrrnl nl f'hrmmttrnbrgy nn,l l tprnrnrnrtd 7hrr,r lrcurirt, Vnlun+c 117, p.r);cs 111-11R, Srptcmlxxr, 1962 i 1V

r\n invcatiF:rlir+n td amrc ayicctc nf the I h:rrru:rculrr~ic:rl xtivily of (.) c•olimnc, :r mrl:rhrrlite of O nicotinc, has JcmonclretcJ th:u. m Ihc anccthctivcJ do6, O-cuuninc Iacks the MHCnr preccrrr :Jlllvlly :ItvrK1:11rJ wrlh thc parcnt cr+mlrrrmJ In aJJitirm, it has been shrrwn Ihal 1 I cr,linine h;rc a Jcrtcccnt aclivity which w:rc rnot aMJichcJ by rndin:ny bhtkrng Jrr.cs (+f itrrrprnc ur Jq,hcnh)Jr:rminr 'lhe prescnce nf Jcprcccrrr activity in the Jcccrchrrtc anJ sprnal Jop is concistcnt with the cuyRcctir,n Ih.rt thc cllctl may Ire mcdiatcd thrrwRh the ahility of (-)-cutininc Ut pnKluct, Jircclly or inJirectly, a vaccular muscular rclaxaticxt. lhe yuanliticc rif O crHininc enrph.ycJ to rnKluce Ihe ohserved pharmacrnhrgical elfccls werc in cr<cccs rif amuunls whirh woulJ arise in vivn from the mctah.rlism of Iclhal nr non- klhal Justc of (-)-niculine. "11I1: ('ORRIi(-1f-.U SIRt/('rtlRFi OF A KI?1OAMII)li /\RISIN(i I-ROM l Illi Mlil i\HO1 ISM OI (-) NI(Y)1INF: " Ily Iterl.ett Mt Kennic, lr , I.cnnnrt It Iutnhull, Udward R Ilnwn+an anrl Srucll I Ghw:uv, I)e r:irtrncnl of 1'h:rrrnacrduRy, Mcdical ( rdtcRc• of Vntvnia. Rnhrurrnrl lr~r.rnnl of thr .1 n,rrir an ( hrrnu nI Srn irly, Vol R1 ral,t•c 4\4H 45'N), 1)cc \, 1962 , lhc tYnrcclcJ clructurc of a kclr,amidc icrdalcJ frnnl Ihc orinc u( Jr+Rs anJ lalcr of ratc JurinR Ihe cnurce of atuJres tin Ihc mc/alwdiern of O nicrdine wac c.tahlicheJ as Ihe mcthyl:unidc uf Ramm:r-( 1 pyriJyl)- g.rrnr)ra rrr<nhulyric ae'iJ lhis evit(encc. IrrF•cther with the eynthccrc r,f the kctA.imirlc, prr+viJcc rqvpnWnilicc (rn .rJJitiunal studrec rm Ihr inlcrmcJr:rry t.rlHdram rd ( ) nicounc OfArr Rrnnlurt Anicrit.rn I,d,.utn('r,nrl,.rnv V (. 1'/trrr nrur.nltrK v. "ti11 ( 11/\NI\M OI I 111 1'I 1\t I I\ 1 r I Iltr rNr r t Itt rl'I( It 1\I'r /K%l1 Il ) NICOI INF" Ily I Ir,yJ V I t•.uL r. ur.I I I' 1,nl•. 11r1 .,rIinrn/ r-l I'h.rnn.r cuh,)'y, Ilnivcrcny of Ir-w.r (0 lrl-r r,1 MrJu rm , Irr...r I rly l.•urnrd r/ /'lrurnrnrrdngv nnA I r(rrrrrrrrrurd 111, rulrrunr r, Vufuruc I 1), Ir.ryr'. ?tlh 11 2. AuRust 19(r2 ( i I R(•. Rrantcc: I trnR ) lhc mechanism nf the IMrvtive chrrmrrtrnpic reslxmse Itr nicraine was studicJ, usinR isolalcJ cal alria prcp:natirrns with paracympalhcUc (vaFus) anJ cympalhclic (persletctlate R:rnglinn) ncrves intact. 7Ix et)ectc uf RanKli- emectnruy anJ rcurpini>•atinn un the res)+rnxe tu nicoline were nhacrvcd. Inhibition of the paracympathetic nervrnrs system wilh hemichnlinium ( I I('-3 ) abolished both the penitive and neOative chmnnlnrpic rcclHtnses Irr nicotine Ilnth of Ihece res(+rrnses coutd again be ohscrveJ a/ter rclrcaled wiehirrR nvcr a 1 tn 3 hrrur pcrirKl 11(•-11 JiJ nrd inhibit the rcclamce to sympnlhctic ncrve stimul.Hirrn nrrr tlrJ rI hlrKk Ihe rccl.rmu• 141 nurrunc aJ mrnistralirrn when Ihe vagi were nnl wmntalcJ CanRhoncctrrmy hrllnwcJ by ncrvc rl.•rcnrr:rlwn r,r rr-scrl,inc :rJmm ictcrcJ hn 2 J:rvs nltirlrchcJ Ilhc INrcitive chn,nrrUrqprr rcclM-n.c tn nr.rrtinc I rnlhcr, it w,rs Jcrn,nclralrrl 111.11 Ihcrc was nu cvnl -nrc rd n,-rcpinrlrhnnc u),t:rkc by Ihc .rrn.r .rllcr'.rnMhrrncttnmy nnA nr rvr rl, )•rncr.rUrrn Ilnwcvcr, n"rrl,inrl,hnnr• ul,l.,lr w.rc .rr1•Ft•./rrl in alrr:r /rrun cr,nlrul :rnunalc :rnJ Ilrr,.r I,r, Irrnt, ,1 ..,rlr rr., rlnn, l hc invulvcrntnt uf thc paracympathclic ncrvuus cyctcrn in ladh Ihe ncVative anJ Irucrthvc phasec of the atrial reclwrncc tu nictrlrnc r,hvialcs the nccccaly of IructulalrnK sympalhctic KanRlia in the hcart to c.l+lain thcse atUync of nicutinc lhc ability of the parasynrpathctic ncrvuuc cyucm tu ipRncncc symti:lthctic nervous syclcm activity hac been JcrnnnslratcJ 1110Ihcr Rrnneur: 11 S. Public Ileahh Service. V11. Other Studies "SKIN (iRAh I IN(i IN MUN(iRl?I. (XK'rS " fly 1)avid 13 I'ilchcr, h1 I) , Warren ti. lrrhncon, A.li., Roy Korsun, M I). anJ lulius I I Jacrrharn I1, P 1 I), Ik•partmentc uf SurRery and ralhrdnKy, l/niversi/y of Vcrmunl ( ul G•r,e of McJicine, IturlinKlnn PlOttlf and RrfIMllrnc/Ivr Surl;rry• Vrdurue 2R, pages 670 6R(/. Ikcenther 1961. ('1".I R.C. Rrantce: lacnhsrrn ) Prcpauatrny lu Ihc s1uJy of IunR hrrmntransplanl:Hitnr in Jr,y,., thc rx. ).r•rimcnlal :urimal td wiJecl chuice, il was necessary Nr 40111.1111 41.11.1 rrn •r fcacJ+lc Icthnirluc of skrn RraftinR and reaclions uf skirn aulryy.rllc rnJ humrr(;rafts in Ihccc aninrals as a hascline for (urthcr tr:rnvlrt.ud.uiun inccchKalinnc. Multiple circles 3 em, in diamcler were etciceJ fnrnr the sh:rved back :rnJ chest wall of mrmRrcl JuRs in areas rcL•uivcly inacccc.iblc ar hilinK anJ ct r:rlchmR Ui.cs td skin frtrm tlrrmK aninlals ( homoKraft. ) wcrc hrallcJ intn six Incparcd eilcs, and an autrgraft into a seventh citc• ac a crmlrrrl, anJ frrur Irr six Jiscc of skin were interchanged tm the saarc cnnirn:d lanlu~ra(1.1. On Rrncs exarninatinn Ihe homnRrafts and auhr}raflc were inJi.linR- uichahlc al K days, and by 12 days beginning tkRcncraliun was apparent in the humugr:Jtc Median survival time of the latter w:rs 16 d:ryc All lnrmny.rafls were shn,RhcJ hy 24 Jays In addition ttr a Jcscri/Nirrn of Ihe steps involved in healing or rejectinn• the micrascapic analycis strupht Irr answer three questitms: ( I)('an nne lell an autufrafl frtnn a hrmurOrall') ( 2)(•an trnc tell infection rrtxn hunur6raft rejectarn? (3) ('an une Jie- tinguich viahk fmm nnn-viabk Rrafl? 1 he answer is a ynalificJ yes in atl casre. Viahilrly is easily eklerrnineJ by persistence and rc~cncralirm of cpi- dcrrnis and skin appendaRet. "ITYIN(i-SPO'1 INIf:RUIiR1iN(1? II:I.IiVISION MI('ROS( ()I'l:" ISy P. (YH. Muntgrrmcry and 1.. 1.. Ilundky, Ikparlmcnl of I':nhlrluFy. Ilm- vereity of 'I e lae, Sr)uthweclern Medical School. O:Jlas Nrnr.rr, 1)rc'emher 16. 1961. paKcs I()59-II)6O (! I R(' Rranlce: Munlgurucry ) 'Ihe authras tkvclaped in their laboratory n 311 clct'urrnic Ilyiny clM,l intcrference televrsran microscupe /nr the rapid Jelt•rmrnalirm rJ teIlul.u Jry mass. 'Ihc sy+lrm rkvieec) has several advantaOcs uver prcvuu,.Iy Jrs critreJ tcthniyuc. ( 1)'Ihe data nn dry macs of whulc ccltc ur crl-~mcnls rd cclls are rrhl:unrJ anJ rccr+rrkJ by Iwrr me/hrhls every I1/ ccr "nJ. ( 1) I he Jry nuss imo~cs rd Ilhc af.ccimcn arc crrnlinur~ucly rKclrlaycrl r,n Ihr mrrnilra Iu1M thninR Itx crur.c of the ciperimcnts (1) '11re vieuaI Inracnratrun rr/ the .Iry rnacs iaraPe makcs („nilirrmnK of Ihe hrighl.•nctl :uc.r rr( nc(- r:rc/rr yuile tasy, and this hcrmils ctattinwrus Jctcrmina/iun of thc dry macs nf selecteJ areas of the ccll t 4I n

rhrr Rrnnr..r.; Atomic linergy ('ummissirrn, Srruthwe~tcrn Mrdical /~uund.rtion, arrd 1)inuxt Runyon Memnrial (-und "AN l1t:IRAV1OtF.T MI('RUHF-AM 'IFI.F.VISI/)N SYStU.M" fly W. A. 1)onner• l)niversity of lexas Stwthwestern 41cJical Schrwrl, t)allas, leAas Annals of the New Yurk AraJrmy of .GYrnr'rJ. Vulumc 97• pages 411R-411, June 5, 1962. (1.1.R C. grantee: P. O•R Mrrnlgranery) "SIMlI1.TANI: .Ol1S ULlRAVIOLET ANI) VISIIII E I I.l'ING SM)1' '1FLF.VIS1ON MICROSCOPY." By P. 013. Mrmtgrnnery, Ilni- versity of Tesas Southwestern Medical School. I)allas, lexas Arrnalr of rhr New York Acadrrrry of Sciencet. Volume 97, pages 491-497, June S, 1962. These papers describe a versatife television system in which an lIV emittin scanner tube replaces the source and primary apcrlure (rf conven- tioual fIV microhcam syslems, and mclhrnls of obtaining sirnultaneous illumination of cellular specimens hy different wave lenglhs nf light whiih presents distinct advantages in biological study In order lu vicw :r y.ccimcn rirmtHanernr.l in Iwo wave IenRths of light two scanner tubes are ernl+h,yed, J n~ cmiFlinR ~JV IiRht and the other visibk light. i(Irher Rrnntor.: Atomic F.nerEyf ommissirm, Uarnon Runyon Mrrnorial ~/ hund for ('ancer Rcsearch, anJ the S.wthweslern Medical Foundation. "Pf:RIOt)I(' FFVIiR, AN FN1I1 Y ACUI I 1?('llON OF 5? ('ASIiS" By Ilobart A Reimann, M 1)., 1)cpirtunenl rrf Mcdicine, lfahnemann Med- kal ('olkge and Ilospital, Philadclphin, I'a .Irnrnran /nrunnl.rf the Mrd- irol Srirnrrt, Vrdume 241, paRrs 162 174. Fehruary 1962. Pcrirxlic fcver was prufxrscd as an entity separate frr+ru other periodic disorJcrs in 19411 and furthcr suhdanlratcd hy the dcscrifilirm rrf 52 cases in this essay Periodic fever is char,rtcrircd by rYhclitivc fchrilc epiaxlcs and nutrmnrnic nervous disturhances in predictahle cycles crf days. wccks or months, or irrcRulirly, in otherwise healthy pcrscxn It also may rkcur in palicnts with malignanl or other chronic disease. It is hercditarv, begins at any time (d life, may last for decadcs, and may have long spornlaneous remissinns and may ccase. About 1(t't, rrr vic- tims died, usually in a shrrck like stale. lhcre is no salisfactury treatmcnt and the cause is unknown. Trrhaccn frLryed no role in any of the victinn who used it. The diarrders nccasirrnrlly al+pcar in familics with other neurntic tendencies or migraine. 7 his Irrgclher with evidence of cerebral dysrhythmia and ahnrxmal neurovascular resp„nses suggests the site of origin to be in the brain "IIfRI:I)II'ARY f'F.RIOhI(' FJ)1 MA. '1111: INI FRRFI AIlON OF I AM/1.IA1- 1'URI(IOI)I(' 1)ISOR11I RS," Ily Iluhart A. Itcrrn:rnn. M 1), Prnfessrir of Mcdicine and Prevcnlive Medicirre, Il:rhncm:rnn Mcdical ('rd- Icgc anrl Ilrnliira/, I'hiladclhhia. P:r ,4r.nrruun /nrrrnu! riJ Nv Mrdnal.Sri- rnrrt• V,+lonic 241, Ir.Res 727 711), fnnc /'1(r1 A nrrml- ..f (ralurrs aurl•ro .rn rnlrrrrl.rtwn tlrlwccn Iw rilsili( rvtrrne nn.1 .r. ,r n...n tvn,.Ju .nrdn•.,l in rhr, rr%rrw Im Ir rnmy epiaKks, long duration with lemfxuary or pcrmanent rcmissiun, and simila. histup,uhrrb-Ric anJ clectruencephakrgraphic changes. Whcthcr this gruup of conditions are unified on the hasra rrf a commrm undcrlying prrrvrKalive rhythm ra are regulated by biohrgic ct4x-ks awiits solution by new invrstigalive melhcxls If genetic faclors are resf>trnsihlr, the chances of prevcntinR or curing periodic disorders by means ullrcr Ih.rn thc application (rf eugenic principles do not seem hopeful. "1111: LOW-FAT I)II:T FOR PIiRIO1)1(' PERITUNITIS." Hy Ilubart A. Reimann, M I). Amrrir an Jrn.rnal of (:atrnxnrrrofoay. Volume 39, pages R5-90. July 1962. Fourteen patients were given a 20-Rram fat-diet; episrxles of herirrlic peritonitis were stopped in four, were mil.kr and farther apart in Iwo, ceasrJ tem(xrrarily in Ihree, and were not aRecled in six. None of the victims who smukcd tobacco noted any influence (if it in his disurder. Alcrrhrd reficved the symptnms slightly in aorne. V111, Review "lllF? A('flONS OF NICOIINF:ON CEN'1RAL NFRVOIIS SYSTEM Fl1NC-1lONS." fly II. Silvette, E. C. 11olf, P. S Larson and 11 D. Ilaag (deceased), Departments of Pharmacology and of Neuruhrgical Science. Medical College of Virginia, Richmond. PhnrmccvfoRicnf Rrvirws, Vol. 14, pages 1)7-17). March 1962. (T.I.R.C. grantee: FIaaR) ll.is is a review of 1 R4 scientific papers and testifies In the nnny lacunae in knowledge rin the neuropharmacological effects af niculine adminiaered tu humans and to animals. The pharmaculkly or toaicningy uf tuhacco- smoking and Irharmaculogy and toxicology of nicotinc are nol idcntical, and often are nnl even comparable. Not only drres tohacco sruake contain many nlher inRrrdicnts, knnwn and unknown, with distinct pharmacological actiuns of their own, hut the duse of nicrHinc contained in the srnoke is not really asccrtainahk. though it is often estimated Since this review is concerned with the neurophanna- arlrrgy of nicotine, the results of "smokins' exQerimenls arc not rnentinned, eaccpt in spcci:rl circumstances, and then only if Ihese srrrn to be equivalent tu resultb obtained in crmlrolkd, yoanlitative eaperiments with nicntine. An account of "smokinR" experiments in general was published in Tnhucru, l: rprrirrrrntaf arrd ('linical ,SrurGrs, by I arsun, I IaaF & Sitvrtte (Williams & Wilkins ('o , 1961 ), in which mosl of the dala on the ncrun- pharmacuhrRy of nicotine to about mid-1959 also ap/rears 'lThe Prrsent critica) review is iksigncd usefully to fill the gap. In general, small drnes of nicoline have a stinmlatinR nclirm r•n the crntral nrrvrru+ system whereas largc drnes dePress '1 hi. rcvirw rnvrrs sl nnl:rnrrrus ar tivity, crrndilioncJ rctlexcc, IearnrnF, hiRher crrrhr.J lurnt' ti.rns, mcdutlarv funrtirrns, cerchcllnr hrnctions and spircnl tunrriom p, n, ~i .~, ~nn.. . ., ~,. ~. .~. ... ...... .. ~ . ... .... ... ... . .. .. . _.. , . - . (S) 41

Recipiews of (;ranls I I•IlnwinR is a htt 1if :111 rccipicnls /+f Rrantt afaprlwctl by the Scicntific A(Ivlcr,ry Illr,nd sincc initial Rranlt wcrc maJc in I•Itc IvSJ It sll.ruld hc nt+teJ thit slrn,c nf the pnljc/as have been completed C/IAN76:F. ANI/ INSTITIITI(1N ( I.ARFN('F M/1(:RI SC, M 1), l'6rr/, Cordrnv.nculer 1 ahnrenwv. Vtltrsnt Al/Inlnttlllllnnl Centtr, 1 ns AnRele.; At.nviorr (~hnrret Prr•ftv•.r n/ MtJrr+nr, llnivtcvly or 1 addorni., I os An/eles nNtlll)NV A AIRANICI, Phl) , l/r rrr h•r Nuf.u.,m nnJ A(. fah.rlu Rt >r,r.rlr /lnru,•n, the WrndrrJ Iputle Rel/r( 1nnnJelnM• Ihe Hurlt 1.nmda lion RchAbdhalrun ( rnltr, Wfule Plaim, N Y. t PROJF.1"T TI71 F. (••('•• rdrrr. p.••brt .n.yr.bl •.L• rwdh.wr ••tlle..r 4.1 hrrw r•.r•I,.A.d Ar .Ar r••+•rrl Meaturemenl of the fnnclinnal al..n.a nf the human heart by freqrnent Y al•ra Irum analysn of ils vrhrauon:J tntrRy (C P) the tRtcl (il nitofhnr nn pr.•Irln .nJ amino aclJ mtcaMrhsrn rn hr..n.ma F I ANCI I AKOS. M I). Ph I1. A.r•• ri,rrt rr••lrlr••r r•I Hml„n tlnivrraly Sth(Kd of Me•Lunt, R,r.l„n 1) MURRAY nN(:I VINI', M I), r••• (rrsur n/ /'vrhn6.A•r. llnirtrvly of Nra convn MtJn'..1 S<LMN•I, MnJ/\.M S1IPIIIN M AYRIS, M1), ltnrrr••r of Rr.rartG. !>rprrcrnrN n/ rulv Phrunfn,yy, Saml Mnh.els 11osr11.1, Ntwarl, N I (now 1)irrclcw, ( uJwp1 Intunary 1 shcwahrry, St Vlnctnl't Ilo.pil.l, New Y(a1 (l1y) FRF11t RIl'K W. RARNI.S, 1., M I), Pn 1) • A rn.r i•ur P-/nr.•r n/ Alr Jn rnr und Ph yurdnRa al ( Armiury, Inhns IInP1in/ University Sche.d uf Medi eine, Ra111m<ac. MJ (nnw PrnfH4•f of MtJrc,J Sc.cnce, Hlnwn Ilnivrnrty, Prnviclence, R. I ) RAIPIt S Rt(KFR, Pnl) , Arnrrrnrr rnr/nrnr nf ( htnrnr.y. lhnver.ny of I/nualnn, 1luualun, I t t SnM1/1/ HI111 I. MI). ft. rrn.•n.•/ c 1'tulaJrll.hinlirn tr.l Ih•.p0.l Or tn r1! fJ Rrir,.at nf talrtholaminra /rnm Ihe Lo I../rJ hrarl P.r/hulncn .rn.+nrmic aludy r•f ttl/ular chanltra in hunl.m hrontlu 1( / Mc.raatmcnt ul :.Ivrnlar nrlrrl.rl n•IrnRtn Ras tMr.rnalnRul•hr 11'/ Jrlltrtnte by the rule of hyperrlasia in Iiaur ttaM.nse ta chrun-ic Jam.rze (( 1'/ An invealiRahnn of Ihe apr.lr:,l +nJ chromatutraphic rharatltrlalita uf nnnnah.hyJrrnarhnnt /1'I Iht rRr.t ul nwd.nr un..u•h.•i u.u.•ru.n In the prr.rme r.l rturrmt, rn.l /br r/ It.l u/ nnn/rnr on tra••nuy bL.wl H•.w .•f Jr.~a v.nh .r•r.•narY n..r•rt•.rrnry 11 1') n.•1•1•..I,y.y 1 n..I. r•1 1.. ,•n •.r ••r n n„ l/ln rn,•n.n Y arl.r rC. .•nA ..•,r 1.•. 1rt....... rllrr 1, u/ on n4nr m hnm.rn . rJ .rnr mA I•1.•vn.•l t/Ic.l, rd .r...,r~••n u/ 4 J GRANIRF. ANh INSTITtrTII/N IIYt AN A RI( KFRMAN, M I), Arsn fiotr ('linanl /'r.r/rrv.r of MrJnrnr, and AI VAN I RnRA('ll, M t)., rtr rtrrd (Ytnirnr rrnfrrcn. n/ M.Jirint, lblkr'e of Physici.ns and SurReom, ('olumhia l/niversity; ('olumhi• Re usrch Service, (:c.IJwrter Memdial /ltnpilsl, New Yor\ RI('11AR1) 1. RIN(1, M 1)., Prn/ec...r of Mrdrrne and Charrmnn. hr/rprmrnl n/ AIrJu rnr, V1'syne $Inle Ilniversily (nllrge of MeJ,(,ne, Iklcoi/ FRLI) 0 R(K'K, MS, Prrl)• .trnrnr Cnnr rr Rrvar. b.trirnNtl. Rotwtll Psc1 Memntial Inalilule, RNd1.Rn.) Sla- hc.n, Spmwilk, N. Y. /see Mnrwe) 1•KIIJF.(T T1T1.F• smoking nn ternm choltslerol levels of chronic smotert (P) Non irrilsnl cigerclle fma1in11 r•.ilh crh fervslinnt on the urhon m.a+nuJc eoncenlraitrn of arterial hh..l and .Ireolar Ras after 1rnnlinR wilh inhats lion .nJ ./ler won inhalachon .moking The tf/ecl of fmolinR on the cornnary hlnual Ibw sm/ cerlan ph.aca of myrs earJial me/.hcdiam in patienl% with arlerioaekrolic or hypalensive urdr ovscul.r di.e.se (PI Slwliet iw cellul.r physioloRy of hearl mrnck (P) Mesiwremen/ of cornnsry 1drr>,1 flow by mesrn of r.dinacliv.IrJ nlhurnin 7he effect of 1nwrhinR on tnrlnlary hltM..I flow in palients wilh ar/eric..clero/ie (rarl Jisttast anJ Ihe e11ec1 o/ mt.Nlnt on 11orage of aminea in htarl muscle /1' ) Measurcnsenl of tnrnnary h11wsJ flnw with 11 syllem usinR comciJrnte cunnl ing ; Ihe eflecl n/ nicolint and ch.InRe in hearl rNe tsn crrJrac mrlnF•ulnm nJ related fuh)eclt 1 P ) tn.elliR.lion of the hinlrrRi.al tOrtla nf ciprtlle tmolse ((' P/ /AMI:S 1' H(INNI'R, Pn 1) , r.rr/rnnr n/ Ru.lu~r. ('slriarnia lmlilule of Iech Un>'yms/ic du.lv of rne/hylalrun rru.linns in Planl lissue 1(• 1'1 ndnRr. P.otkna, ('s1 1(.M<1 AfIWFRY,Prrl),rrrwiJrRrr •.Inr /nAnrnrnry. ( lrtrnrlfrr I)r/rrrf nrrnl, Norlh ( srnlrna Slale ('tdlelte, 1I11S •nd endrin residurs in ciRartllt sm..1e 1(' 1') RaleiRh. I(YSI'F RR//71 K, Pn l) , r'rn/rrtnr nnd RioloKic.l thnrat/rnslrts if mtn .InJ rhru l h,Ur.nen, nrl•.onnrn/ ,•/ rr,.1•nluRr, I thiflh 1lniveraly. Hr/hltbem, 1•s .molintt hnhits 1/ 11) /~ M H1I I'I, M 1) r.,•/r.•,•r •f !•.rdr••I /'nlh..InRn nrulnnu. a/u•IY rJ .rllul.r l/n/vrrslry .,/ \•nnhrrn / ,Jdurnrn tlu.nRrs in hum.rn hr.u..hr 1( 1 S.hrrd c.f MtJlunr, 1 Lr,/ I•..rb..!„crrr, t.rs nngtlrs I r•n•,ry 11.•.1•rl„1, ( An prlrs Sru•/y u/ 1r.nr .ntl.J rJ p•rhn..•r nry •n.l h.rr U..ut /•y '/.r /,•sr •rw/ thtnutal mrllu.la 1l1 RI( IIARI) It 11)'1 RMIIM 1'n 1), rrn/rnnr .•/ c7rrnu•r.l. MnfuR.m St•Jt I /nirtrsily, Fast 1 .rnarnR Ihn.yNhrsia rl IT.c pY•rJurr rrnR uf ni,r• Irnc Il1') ' 45

i i i C/11,NIFF: ANn INs'fl'f11TIl/N SISt1R M IM11Y (A11111, Pnl), ( hnrrrnon, ( hrrnrrrry flrp.rrrnrnr, krs.s ('ollesc. WYsarn, Maa Wfl I 1/1M FI ( ARNFS, M 1), rro/rawr n/ Parhol..Ry. Universily of Utah Col- Iree of MrJkine. Sa1t I ale (ily. 1 liOPO1 D CFRF( F(K), Pit I)., rro/rr. aor o/ RiaArmnrry and Nwtriunn, llniversily of Puerlo Rico, School of Pu(/Jt:l-r -rln.r•: ( rxnp.rn.m uf anJlytiul mclhr.Js by Jelcrminrng Ihe percent retuvrry of arsenk aJJcJ at Inown levels to tn- bacto samrIes anJ a Ino.rJ uuvey of the amorml of arsemc present irn Inbacco .amplet from a wnk varrely al sources Pathologic anNUrnic studies of cellular changes in human bronahr 1(' P) A study of early chemical (hanges in the lungs of lumdbearing rals and mice IC--P) r CRANTF.F: AN// INSTIT(yT1()fV A/RFRT UAMON, M/), PuD, Arrir.' mnI PrnJrsvfr ol AfrJno/ AnrArrqtd- nRy. Dr/'~rrmrnr of Indrrhwl IlyRrrne, Harvard School of Public lleallh, Boston. R. F. hAWSON, Pnt). Pr../rn.nr n/ Dntany, Columbia Univeysily, New York. ANI)REW S. DIRNFR. Pn D., E.rra- rirr, rryrAo Rrnea>'rA, lhe Age ('enler of New Fnglaed, Inc., 6oslon, Mass. (see Whislin) PRI/JF/T Tlll.t•: Body form, srnu\in(t, anJ altuhul cnn sumplion among Ilahan Amerrun men (C-P) An in.rNilalion of Ihe rnclaholism u/ prriJinc compounds in the lohacro planl (C P) PsycholrrRkal nJ behavioral charattrr Islict of inhalers among smukers (C P) MeJkine. San luan (formerly Profes FUWARD F. IX)MINO M D, Asarrlnrr Fflreu of tobacco smule anJ nktNine tn+ snr (f Riochemis/ry, Fordham Univcr rrn/rtrnr of PAarn.arnfoty Universily the central nervous s stem (P) sny, New Yo.1) . of Michigan, Ann Arbor. y Wit I InM 0. ( I ARK. Pn D, l hniral I flecl of nKWine and rrlICd suh.lances RAI Pit L. IX)RFMAN, PN 1), Urrrrrnr (ivalualion of tol..cco /un.umpuon un AtuMrarr Pr.r/runr of PAyu.JaRuol on amine levels in the nervrws syslcm o/ foMa.rrnrrr, Worchester Foun.ta- the basis Of nkW/ne nr nrcrNme rnrla (Armi+l.y, t/mversity of ( .Irfrunia IirM for 1'Rperimenlal flWloty, Shrews. Mdiles in br.dy flurJs an.I carl.ue mon Medical Cenlcf, I on Angeles. 0 hury, Mass. o.iJe content of blcwn) HANS r. ('1 ARKF, 1)St', P.nJrnnr n/ NrorAnnurry, lulumbia Universlly ( Cnllcge of Phy.kians nd Cur~teons, New York (rrtneJ, see (itNlschah) Proleolytie activities uf the white blood cclls of rnen ml the effect on whrle hlrroJ cell activilies of cafcrmritens, nunrlrun anJ tither mfluencts ((') 1OI1N W. F('KSTFIN, M 1)., Arrluonr /rn/rstor of InNrnnl Mrdrrinr- ('ollee of Medicine. Stale llniversily of Iowa, lowa ('ily. Responses of the peripheral veins in man to the inlravenuus admrnrstratmm ti/ nicotine IP) Fool bloal Row respun.et tu srnn\ing in )AY 1) ( 01 1 MAN. M 1) , Inrtru~ 1- in Ihe effect of luhactn tmuArng on nubi the presence of hyprrli(amia .md hy ptr lention AfrJu rnr, Massachutetls Mtmori.l llnsprl.ls, Buslon. /rve skekl'l mus[Ic hhMMI Ouw as detcrnuncJ hy the radhuiwlopc Jrsappcaf ance lahuiyue (I'/ NANS 1.. FAI K. Pn 1), Srni.rr Rrvo.rlr Asnrciatr, I)r~a runrnl of I'ath.rlnR., Fs.minalion of ciRarettr paper •nJ trRarel/e smole condensales lur aruur.uic llnirersily o/ Soulhern ('alifornia polycyclic hydrraarl.ons (l ) f) 1)nrr ( ()MR(tF M )111I(IS 11 1. lhe effcct (if srnokinft u{r+n airway resid I r» Angeles. (m+w Sclwtui of Medicine , . . , , ( nrdiovaaru(ar Rrnracrh fnrnndr rnr ance 1( P) . CaccinrrRene- NCaJ ('hemhlry Section A compilation of Rutwescence speclra u( . , of (llrfocnia MeJrcal ( Cn sil llnivc Analysis of case hislexirs on emphysem . , Nalional (ancer sit SlnJies Branch P^lycrtlk aromatic hyJntcarM.ns and y r ler, San I rantisco . In./rlute, Bethesda, MJ I closely related comprronds whit h are u/ Interest in the atudy of air rydlutants 1)FAN M. (-UNNURS, M1), Arr.Kate Durcrnr, Urpuelmrnr of I.Anrnrtrr y Mrd.cinr, SI. Mary's Ilospiul, M.Jison, Wis. P1111 IP ('(X)PP.R, M 1) . Chief. Surpunl Srrrire, Velrrans Administralrnn 11tnpital, Rrons, N. Y; C'6nical Yr.~/rrr~.r o/ .SorRrry. Albert Finslcin MrJiul ( ullrar, kronc A study of the alteratinns in t)te human bronchial wall occurrns with aRing, with parlrcular emphasit on elastk lis- sue chanRes anJ associateJ chanRes in the br.uvchial lumen sire A sludy of Ihe effects of ciRarelle snwr\ing on luvelt of ppstrre acrJ, pepsrn, anJ uropepsin ((P) A slu.ly of t)K effect of UlfacFS Uf Irrl+ar (n IM tulnrres of IUnNN anI n.Mnta1 cells. Animal Iramplants uf turnur bs sue horn IrssrK cullures 1( ' /' / S/uJy u( tnml.lned efletl uf m'r.ICJ nral agrnts an,l rnvinrnmrnl.J (alu.v, in (1uArnI..rnuu.arns rn,l L.hat,...n...1c, on Ihr lraahr.dnnnthul rrre nnJ pul muoary y..nrmhyrna uf u/rrunrnl.J anunals anJ in Us.nes in urg..n tulnuC 4(. 1)ANA 1 FARNSW(IR111, M 1), llrnr. A rlb.vr rr..(rrr.u n/ llyR/rnr nn.t flr rrrrnr n/ f/nnr.urr llr,dtA Sr.rurr, IlarvarJ l/m.rr.rly, ( amhriJge, M.ss (ate llcalh .nJ MtArlhur/ , and eiliarelle amohe in relation lo lung c.ncer rliolt~y (C PI The effects of Inhaceo smole cnnJensafe on Ihe defense mech.nisms of the ut in deloaificalion of p.>tycyctic rornarrc hydroearM+ns and Ihe eumprritive inMhilion between Ihe prdytyi chc' hydro earlKMt in eom)ensrle wr10 Irga1J to eareinogenesis ((1 lht phyueal an.l ehemical prnperties r.f mutus unrlrr mwrna cnnJrlu.ns and following e.prnwe tu .rrus.s unlants ((') PHSOnaldy rnd smulu~e tn trdlrar Rr.d utlrs, . M1llern yr.u /ul/n.. up .IuJy I('/ 47

CRAt'(TF.F. AtrD ItvSTITtrT'IOtM FRANK C FFRCUSON, )s , M D, ('Amrman, !)rporrmrnr of Phurmocololy. Albany (N. Y) Medical Collelre. R(/S.SI.t L S FISIIFR, M I), ('hirf Mrd. r al f ramrnrr. Sr.le of Maryl.nd; Pro- fruor ol lirul Mrdn rnr, (lniversily of Maryland Medical Schoot, Sallimote. D L FRFFDLANDFR, M D, Dirrctor of ('anrrr RrsrarcA, Mount 2ion Nos- pilel and Medical ( enter, San Fr.n- cisco. (deceaseJ, re. French) FRI'nF.RICK A. FRFNCH, A S, DDec- r.,r of ( ancrr ('Armorherapy RnrarcA, Mount lion I/oepital and Medical ( enler, San Francisco. see (Freelander) JACK FRFUNI), M 1). An/rronr Pro)es- tor of PA•,.na.nfoey, Medical ('oUege of Virginia, RichmonJ AP.l11UR FURST, /'nl), )1or.tnr, In inrurr of ('hrmfcal Rw.logy, lJnireruly of Sen Francisco (7t:ORO1! O. (iFY, M 1), I)nrcror, Frnnry-Howrfl ('ancrr Rrrrarrh IaAorad,r' AsuxLaN PrnJrssur of Srrgery, /ohns Hopkins llniversily School of Medicine, Raltins.xe, Md. 111OMAS M (3(K'KI', M1), Arro.rarr Prn/rn..r ol Prr.rnr,ee M.dirrnr and ( ummrniry llralrh. Selon 11d1 ( ol trter of Medreine and 1kn11.Uy, /trsry ( rty, N / IRA (;(IR1', M 1), Pr,.frrn.r nf PrrA„! ,.er, Ilndrwr l/nr.rrdly SaMnd u/ MeJ itinr, I'0rhoL"eur. Masuchusrlls Me mru ial / ln.pilals, 11rn1nn PRO)F.(T TITI.F. FRtcts of tobacco smoke upon the func- tion of the cardiuvascular syslem in animais and man l(') Palhdotic anatomic study of cellular ehanfeet in human bronchi (( ) Fxperiments on the possihle carcinogenic and cocarcinolicnsc actron of tobacco products lC- P) Carcbosenkity, coearcinotcnicity and .ntl carcinogenicily of dietary factors In rclation to PulnKSnar tumurs P sibk interrelatronship ofrtobacco ba.n ses asd dietary faetors (Temrcal studies on pyridine bases includrng nucin analop (C) Correlation of multitechnical procedures performed on the periFlseral circulalN,n of normal individuals in recumbent and erecl~ dinns and after e.ercisc before and.(ICr sharn and actual smokins ((') A study of the eRecls of cigarette smoking on the peripheral cuculatir,n of inJividuats with arteriosclerosis oblilerans •nd other ptripheral vascular Jrseases, uldit.nst multilechnical proceduret (C) A controlled arudy to evatuale the effect of admmntcatsun of trace rsselals (with or wilhouf espwure lu ciltarene smoke) on Ihe incidence of pulmonary adenom.t in Strain A mice Fellowships for studying the culture of human lung tissue and the effects of known and pcnsibk carcinugcnic agents upun such tusue (C P/ ('haracteristks of normal cell gsowth in culture in relation to invasrve crunnma, especially in the lunoel A studr of Ihe nssd anJ pheryn~eal haelerial fkMa of srnukers, nun smokers •nd to snwkcrs Inlrrathon. uf arlrrial •.IA murrrp..ly urcharrdrs 49 i CRANTFF. 1P(IF INCTITl1TH)I'( GI R I RUDF Y. G(/fTS(11At I, Pn D., Alvrranr P,ofrr.lnr of Rr.rhrmurry. Drporlmrrsr of Parh..lnl(y and MnroAu./ogly, Ihe Rockrfeller Inatitute for Medical Researth, New York. (see ( larke ) A('IARK GRIFFIN, Prs1), llrod nf Rr.rArmin.y )Irporrmrnr. M. 1) An- derusn Hospilal and lumor Inatitute, l/niversily of 1 ea.s Medical ('enter, I louston. MORTON 1. OROSSMAN, Pu D., M D., Asrr.rsorr Cfinirar Profrvor of Mcdl- rrne, llnivenity of California Medical ('enter, I.os Angeles. ('ARL C. (1Rt1117tt, Pn D., M D., Asso- cbre in PAysiolr.py and PAarmacoloRy. llnivenNr of Pennsylvania Qr.duale Sthcxrl of Medicme, Philadelphia (nose I«turer in Physiology. University of l/ons Kong) 11 R. HAAt7, M D., Profrsior, oof PAer- nsarnlotry, Medical College of Virginia. Richmond. (deceased, set l.rson) )(/SFP11 11. FIAFKFNSCHIFL, 11,11). I)rceeny, (a.diopu/mr,.w.y F/nir. t.n- kenau Norpital, Phitadelphia. RI('IIARD 1. IIAVFI, MI), Asrirr•nt Profrssor oJ A(rdwrnr. University of California Medical Schod, San Fran cisco. IIFRRFRT R. /(AWl-IIORNF., MJ)., ('hairman, DrPortmrnt n/ SurRrry, l)niversilr of Pennsylvania Oraduale Schtsol ot McJicine, PhilaJelphia. ('I.ARK W. IIFATII, M t)., Prnfrssnr of Alydreinr and Dorrror of IlraltA trrr i.es, Tufts Ilnirersity, Med/o.J, Mass (see /vrnsworth and McArthur) PAI/IINI? 11F/71•R, Pnt), Rrrrnrh Ars.nrdr /n l'tr,4uiry and ('era+Arm nnr, San I-r.n.rsco Inaitule of Mn1I t.l (Sakneet, San Fran.ictu (tre Rith .rJs) PR(/)i:(T TI7l.F. Prottolylic activities of the while hlr.n) cells of man and thc effect on white blood cell activities of carcinuteens, nuIrition and orher influences (C) lAe effect of eepofure 10 c/leNetle smnke on the induction of cancer by chenuul compounds (('- P) The effect of smoking on certain Aaseric fssnctions ((') PA.rrrsacdosk studr of nitotine nd rt lated alkaluids ((.), Preparation for publication of a MMok on Ihe bidofZic aspects of lobacto and smoking (C- P) Measwenxnt of ccwonary b1ooJ Row, cardiac work •nsl cardiac o.yAen and earlw+lsydrale metabolism in mumnlensi.e subjects before and after inhs venous nicotine and e(ler smukin/ standard cipretles ((' P) A study of the effects of sMoking and nkMine adminislralirm nn sympalbo adrenal function .nd fa(ty acid meu holism ((') Attempts to Induce pulmunary neoplaons in esperimenlal .nim.Fs hy raposure of the Iracheohrurschi.l system to In bacco .ow,Ic 1(' / Persnnalily anJ amokinp in cullr`e llrnJ uates; a hlletn ye.r frJlow up stuJy W- P) A ennspardive study of rarly histuloltn a1 •nd (1NA ch.nges in the eprJernus uf Iwo srraini of mite (l 1) Ntsks .rn.) Swln WrMtrtn/ frer Jady a(qdKa Uom of whole ciparrllr .nu.ke r.m drnsale (ah,r.e anJ suurhmr,l with erurrws oil) and d,r ur.rnnRrns la nselhylthulanthrene anJ 1,1 t.rnrp~ rene (C) 49

CRAriTFF. ANO INSTtTIrTION 1 A,WR( N( f L III STFR, 1. , M/), rr.rJrunr and ('Ira-.n, l)rparrnrrnt of OMrrrnu an! (:tnrndrty, Medical (bllege of Suuth ( arol(n., Char!calnn. I Rltl' ( l1RTIS III)FF, PND., Mb., Prn/rsuor and (hurtman, l)rp.rrmr.r of NrarolnRiral Sr irn(r, Medical Cd- legc of Vlrginia, Richmond. R1155F1 I. 1. 1101 MAN, M D, ProJn- tor and Ilrad, l)r(.arrmrnr of Patlrrrl- nPa 1 ouisiana State Uni.ersily School of Me.hcine, New Orkan.. (decc.sed, rte Mc(J111 .nd SI(ong) I RI I)t)Y /1()MNItR(ll'R, M(1, ),trtl drnr, Rrn Rese.rch Inshlule, Inc ,(am hrirt. Ma.r I RORI R I W 111111, Pn I) , 111.a .nrr Prnftq,rr, (Irp.urrnrnr ,•l R,..I,•rr~.J S.rrnrr Nrul/lwnlrrn t/nrar.rlr, I. nUon, 111 (;1 OR(iE ln( ()NC(IN, M I), r...l.r.,.r und )lrad of RoJ... InRr. l/nnrr•il/ n1 Soulhrrn ( ahhwnu tichrwd o1 Mtfi cme, I os An/eler 11 RRY IIARI IA(-ORSON, M 1), lli rrrrrw n/ F.fr(rr.•phyrrnl.rRy. Ntw Ynr1 Fye .nJ Foor Infirmary, New Yrwt )tll 111S II IA( ()RSON 11, M 1), Alrn rwlrr rrnfr.(or n/ SwtRrry nnd Ihrrrlnr n/ .SurRwal Rr(rarrh, ( rdltRt nf MtJi cine, l/niarsily of VernxxH. NurlinR ton M(IKRAY F 1nRVIK, Pn1), Arlr»i,dr rr„/tlnrr r/ rhnrnr.r..L.Rt. nn.rrl 1(nar,n 1 nllrttt uf Mr.)xrnr, Nrrwl., N V nNI/M1\V A KANUItIS(11, 1'ntt, tr.~ll S. vnrnr N.~.~ Lr N 1.. ).r.n Mc m„rr,l 1.Lr,ut..rr. N.r Il.rl.r.r, Me PR/)JE/T Tlil F: lhe rclalion.hip of the u.e n/ Inl.acco prr>,lucla to Ihe oulcome o/ /nRnancy (C) CereMal aulonomic changes pro.luccd by lobacco snx,le, ni(o/inc nr c.nminc, a nscubol/te of nicollne Patholr.tic analnmic sludy of cellular changes in human M(snahi (( 1 The inRuence of /obacco smoking on acute myocardial inCatcli.xr (l ) Slwlks on carcin(>tenesis and the bio. .ssay o( carcinogenic agents (P) (-ownparative stud/es of eneals of rarir.ut tobacco fmote con.knsatc. (in s11ns of mice IC') Systemic elfccts of three tohacco smote cnnJensales ((') Phnlr.lynamic •ctivation of c.ucinolttnK h)Jr..arl..rna(.) clanJardvallnn o( ..rv L, hnutut. nnlvrnR plu/nru.r, r..r.ly.b.,n nl Ihr nu.h..nnma Iht rr.fL.rn.r rd .rrl..rn tn.n.rnmrnlal L.L.r. .n Ihr Rrnr.rs r4 ntnpt.r.IK Jra..t in IIIIKIl111U\r4 palltnla an,t in ah.IJren• A comparison nf tleclrorttmnRraphy as a meam of evalualing the cflrcl of •asrlarMSlrx1(x drugs uMxl celcbral .nd retinal circulalion wilh other IcchniQncs for Ihis dcterminalinn ((-) I ung homolranaptanlatiun /( P) PII../ Uu.ly .d iuhilu.nir,n t., nr.unnr by mr.ma uI rht.ua mr.nlry. It I tn .Lrr.n..nr lo rh..l .Irnl 1..6.,rrr *1a1. rllnnl.• IhC :r.ll.'rr Irf ...r ,..Rrn/c h/.hrti.uf.r.n. rn /ht aYin and nlhtr Ir..ur. I( I T I /:RANTFF. ANI) /n%TITIiTI()W ARNU11) R KAPI AN PI/ IL, !)OKrrM, I aMwarnt) of Mrdrral (:rnrrita, ('kveland Psychialric Inslilute and Ilospilal. . 1 1 11111 KAl7, Pit D, Maarierr Ptn/.crnr of Snciololy. Univcrsily of Chicago AN('Fl. KFYS, P(r 1), Profnso.o/ P11ya- ioluRiraf IIyRrrnr awd f)Mrrror, (ab- rwulory of PAyaiolngiral Ily~ irne, Uni- versity of Minnesola Sc11oo1 of Public Ileallh, Minneapolis. 1OShPll fA KIRSNfR, M1), Prafri.nr of A(rdwinr- l)niversily o/ Chicago Schord of Medicine KFNNFI)1 P. KNlJD7SON, MD, Ar- rurunr ('Air/, I ahnrarory Srrrkr, Velcrans AdminislrNinn Hosp1tal, Sealle, Wash ; Prn/run. n/ Parbrrf.rRy, l)nivc/sily of Washingtun Medical SchrxA, Seattle. ALVIN 1. KOSAK, Pu 1) , Aarnclarr Prn/rTrnr al (-hrrnrrtry, W.ShinRlon Spuare Cullege, New York llnivernity, New Yort. RO111'RT A K1111N, M 1)., All Souls Ilnspital, Morrislown, N 1. MARVIN KltSc'IINFR, M I), Profrt,nt of I•.rrhnloRy, New York Univers(ty ('n1/rRe of Medicine; llirrrrnr u/ Pr. thrrlrrRy, Fkllcvue Ilo.pllal, New Yocl. ( IIARI FS W. I A RLI I F. PN 1), Ar- linnn/ Lo/rrv.r n/ F;nrv.rnmrnml ll rrrrrtr, Urryrrrmrnt a/ Prrrrntivr Afrdirrnr• IrRersnn Medical ('ollegt, Phit.rklphia I II(tMAS ('. 1AIPPI Y, M 1), Pru/nnw o/ Porh..f.rRt. N.wlh.es(ern l/nivcraily MeJnal Schrwd, ( Arurrnnn, I)rp.rrr mrnl o/ Pmh.dnRr, Wesley Memnri.l 11rrPi/al. ( hicasrr 1'nl/l S I AMC()N, Pn1), I'rn/rrrnr nl L.Rr, MtJn.1 ( nllrRe u/ Vu ~rnu, RxhoxmJ I.cr M.Kennr./ PR/IJF.IT TITI.F. F.uminaion nf 1he utenl of, and con alilutir.nal b.sis for, correlations hc taen the following : (1 ) Usle Ihrtsh dds for quinine .nd 11 N prupyhhroan K/l, (j) conslltutxlnal plerl(tplnllnM to p.IM1/oRic v.riahles, ( )/ ind(.alual di/lertnccs in smo\ing practices, and (4) food dislikes Social and psychological conslrainls on the 1r.nslaion of nx+livts inlu acllun. Thc case of ciprclle unobinF ('haraclerislics of men, inclu(Lng smok Intl, in populaions dlf/eNng in Ihe inc{aknce of coronary hearl dnease IC- P) The effecl of tobacco smuling upnn basal pslrk KfrtliaM/ in man I( 1 Palhologic analrxnic s/ndy of cellular changes in human bronchi 1C P/ The isolalion and Idenlifkaion o( cN lain lower hoilinR cnmppments of u@ •relte snxrke (C P) Po.sibk eflcctf of Iob.cco upr.n cerrAral circulation (P) P.IholcH{ic analnmic sludy of (cllular ch.nges in human hron(hl ((/ 1'Rec/ of cigarette srnule on pulmonary clearance PalholoRic analrmric slu.ly uf ctllular changes in human Munahl It I ( Lc.vIK'a(iun, pr.rRnn.r• •aJ rhulnRlr faclrws uf prlrnuly /umnls n/ the hmIt /'rrparathr.h lur pnhlr, wnnn uf :. th,rrt nn the hrnlnRp' ./v, 1. of luh.l.. n.nJ snxr~InR (( ('1 Inrym.Irc /r.n.f.wmr,rr..o. of ow.unr anr/ rrlaerl cumtrn.n.1. 1/' / thr pprs\Ihlr rRral r,f Irrh.,.... .r r..1r •n.l nwuline on a..urhl. .,.r.l mc(.L.dnro ((.) 51

(:RANTF:F. ANt) INtiTITllTl1/N (iUSiAVF A I AtIRFN71, M fl, Arrirt anl M1n/nrnr of MrliNnr. Sclon 11.11 ( olleffe of Medione, lersey ('ily. N. 1. CF.CII If t El1CIfTFNRFRCFR, PN D., Senior f!lnlotfrt .nI C-Arn.lrt, Sias Me- mori.l 1alwwNoeies, tirooks Ilospiul, Srookline, Mns. FS)) N c) 1 INI)SI-:11t, M 1), Arrlnanl f rnfrrrnr of Surtrry, llnivertiry of Minnesata Medical School, and .Stag /A.,irren, Srrtir.t Snricr, Vettrant Administntion I(ospiral, Minntapolis. ROHFRT FI 1INNFI t., Phr 1),Auneiorr lrn/rr,or of ('Armnrry. Unirervty of Vermonl, Burlintton (now t Nal/on.t Science FounJahon, Warhintton, I) ( I II)iRtIF.RT 1. 1OMf1AR1), M 1),C'nnrrr RrrrereA Innirarr. New I:ntland Iks eoness llospital. Soston. I P. lONO, PN t) , Arror4.lr Prn/ruor of PAarmacoloty. St./e llnivenity of Iowa ('olkte of Medkine, lowa City. KENNFIIt MFRRII l. LYNCtI, M D., Sc D, I.L D,(-Aancrffor .n/ lro/r,ror of larAoloty, Medicsd Colkre of South C.rolin., Charleston. DAVID P.. MANN, 1. , PN D, Arrncia/r lro/rrror of lAm.nxnlntf, Temple Uwivenily School of Pharmacy, Phila- delphia. ('t/RISTOPFIFR M. MARTIN, M 1), Arri»ant Irofrrao, of Mrlkinr and nbrcror. nirlriow of fnfrerinrt nb- r.rrr, Setow 11a11 (-olkve of Medicine, )trsey C111, N I. ('l1ARl Ft C Mr ARt/1l/R, Pul), Prr• A..Ly.rl rn rAr (lnl.rr,itr ftr.JrA {Ira... II.L.IJ In/.r,\Itr, 1 .m Ar•ds• M... 1..r IIraA •nJ / .+n. .....1. / PN(/)F.(T TITI.F. Srudies in bronchilis:  correlateJ inve.ti plioa of (a) the eflecl of smokmt on the bacterioh.gy of the re.rualury tract of humanr, and (b) the effecl of cigarette smoke and sulfu, dioude lair pollur.nt/ on the ckarint of bacteria from the respiratocy tracls of small .nimals (mke, rats) (P) A cxrxlatcd histological, cytoloRical and crtochemical study of the Iracheo. bronchial tree of mice eapoxd to cig. .reNte smoke (C-P) The Internlallon between influenla virus inlections, eapo.we to cigarette smoke and other factors in the Je•elopment of pulnmonsry and bronchial iesions in mke Measurement of Ihe drRelential blood /bw In the mammalian lung during the acute period of smoke inhdation, usint Iht open chest animal and the method and principle of famma emitting r.dio- .ctive graded mrcrosphetes the osidation of nkodine by taseous nsyfen mechanism, product% and ki• nrt.cq t(' P) lhe .wosidaliun of nicolint (P) 1'pidemfolotical faetnrs in tunj cancer Cardlorasculfr effecls of nicotine (P) F.nvironmental factors and pulmonary dis- ease. I Asbestos duat ERect of tobacco smoke and tobacco ren- iduts on melhykholanthrene induced skin carcinotenesis in mice ((') Prrnihle Interactions of viruus nA sub- stances in tobacco smoke c(,rukmte (r P) Socl.l and pt r.onal drrtrndn.na nf n.w,kin8 hrh..i,w I(- P1 IIK r.n..t mr.1i.1,IM .+f ..n,.kln~ t.r t CRANTF,F. ANI) IPICTITLI•TIONI ('NARI.FS R. M(CANTS, PN D., Aaro- ria/t lro/rrrnr o/.Sod,. School of Afjrl. culturty North Cardina State Colkfte, Raleigh. IIENRY C. M(C11 t., ). , M D., Arrint I(rad, /)rrort.nrnt of ParAnlotf. 1-oui- si.na St.le University School of Medi- cine New (kte.ns. (see llolman and Strong) KFLLY T. McKFE, M 1) , Asrorlarr lro- /rrro. of Mrd/rinr, Medical C'dkte of South Carolina, ('harleston. I(F.RSERTMrKFNNIS,1.,t .f)., f.o- fr»nr of PA.rmerololy, Medical ( d kffe of Virpnia, Richmond (see Lar- son ) VI(TOR A. Mr Kt)SICK, M D., Profrr- aor of Mrdk/nr, lohns Ifopkins Unl- versily School of Medicine, Saltirno.e, Md. tAMFS C. MIt I.ER, M D., PN D., Iro- (rrror of lrychiatr>- and lrytArdoly an/ Dlrrclo., Mrntal I/r.hA ReurcA Irulltuff, University of Mkhit.n, Ann Arbor. 111/CN MONT(IOMERY, M D, Aranrl- alr lro(rrror of MrJkinr, llaJvenily of Pennsylvania Medical Schod, Phita• delphia. P. O'S MONTGOMERY. )e, M D, rro/r»r.r of latAoloty. University of Tesss, Soulhwestern Medical School. Dallas. GEORGE 1°. MO<)RF, Pw l)., M D, f)1- rreaN, Ro7weI1 Park Menwwi.l Insti tule, RuNalo, N Y. (see Sock) III/RI HY I 1'f Mt)1/ UY, M 1), lr.•fr,- t.v .•/ A(r/u /wr .nd f lur. fnr, ('r/rn. Rr.ruarn•y IoA.•...r.•.y. Itni.erdtr of S.wthern (Cahtorma Sahool of hlcdl dne, t os Antcks. PRO)E(T TITI.E Arstnk conlenl of u+ils and absorption by the tobacco plant W - P) The eRects of environmental facto.s on development of .thero.clerosis as rr .eakd by aulopsy of accident victims Sludy of lung function in rmoktrs and non.mokees (C- P) Firrywnik IransfnrRfal/am7 of nicotine and related compounJs ( P ) A combined ifenelk, physiologic, clinical nnd epidemlololk study of Ruertjer's dise.se (C P) A study of Buerger's disease among the lewish ethnic groups in Israel The behavioral eRecu of smoking under Mreu (C) Influence of tobacco smoking rro the blond flow of skin nJ of muscles of e.Iremilies in sympatheclomued and r.nsttnpuheclomrted sub{ecls I(') InRuence of nkotlne li v) and tobacco arrwling on blood flow in human skin .nd skeletal muscle (C P ) In.esligaion of the role of the nucleolus in the normal .nd the malignant cell /P) An inveslit.tion of Ihe phy.inlotical ef /etls of direct inhalalu+n n( loh.cco smoke hr laboratory animals anJ the study O( the b/oh.Sic al rc.ponK of laboraory animals to eonlimr.w in gesliun of diet tobacco prrrturl mie t.res (C ) A sludy of the eRe.H of smnUnt .+n rul rnnuaty funcl.on tc' rt 53

CRANTF.F. Aivl) t'vtiTITlyTION W1l ! IAM S. MURRAY, Sr 1)., Rr srmrA Arrrrrorf- R...cru 9 )ac\.cxr Me(ncxial I abcx.Inry, har Ilarbor, Me IX)NAI h M PA('F, Prr D, Prn/rnr» of PAysloloRy anl Dufcrn., Inttrrv/t /oe Cf)Ir)ar RrsrarcA, University of Nehr•s1a. I incol•. FDWARD W. PFI IKAN, M 1), ('Aatr- man and Pro/rnnr of Pharmarrdolty and F.Pfr/mrnra) lArrarvrrlcs, @Ioston University School o! Medkine, Boslon. (1TAKAR 1. POI I AK, M D, Prr 1). F..rcunrr llbrrlor, 1)o.er Medical R~ search (enter. Lc, 1)over, Ikl. MORRIS POt.I ARD, PN [)., f)irrrlnr, L.o6and Lasorarory, Universily of Nolre t)ame, Nocrr 1)ame, Ind C. M. POMFRAT, Prrl), ))rrrrrnr o/ Sbintifal Rrtrmrh, 1'ua.kna I ound. (ion for Med.cd Rese•rch, PasaJens, ('•I. It R PRATT-TIIOMAS, M D, 1)ran and Pre/rstor of ParAr,logy. Medical ('ollage of South ('ardina, Ch.rleston. MARTIN S. PRO77L1, 51., 1)1)S, ('Arr/, Drparrmrnr o/ Orar rarhr+inRy. RTarttand Medical ('enler, Newark. N ). 1'It()jF:(T TITI.F. 1 hc production of /enelically controlled •nimals nJ lumors for uu in e-speri. mental research on lohacco in relation to health (C) Studics of the mammary tumor promot- iwil Qualities of a virus hYr principle. Weedet varying hormonal and ./ine co.ditions ((') Fellowship for training In tissue culture lechnipues (( ) Study of the eRects of tobacco smoke constituenls on v.rious stralns of tis- sue cells cultivated in vilro I P) A study of shucture activity relatinnships among drugs wbkh aRect nkminesensitl.e physicdogical n.nhanisms (P) Possible eRect o( tobacco derivatives on arterial and myocardial lissue cultures (C -P) Chemkal .nd viral carcinogrne+is in the •senk ("termfree") nimal 1411ow.hips for sludying the culrure of human lung tissue and the eflrcla of known .nd'rn.ihle carcmolenic agents upcwc such tlssue (C P) P.Ihololie anatomic study of r ellolar ch.nges inPuman Atonchi W P) Application of a new bioai+ay Iechnique in esaminalion of cigarette smole c(in densa(es for possible carciuolens (C--P) C•rcinornesis in the laboratory animal: • comparison of species response to slandardited siles of •pplicalion A comparalive study of the effects of )rnethylcholanthrene and ci(aretle smoke condensate •pplird tu t).e oral lmurs of Swics mMe 11(R) cunJi liueed with •Icohd •nJ/nr urbun IelrachloriJe a, cn WAI 11:R RFI)IS(/1, M D, New Yorl V..col.r rfcpmKS to tuturcn .rnn\in• rJ I/niverut/ Reu.nh Servke,Oo1J.ater tn p.htnlr .dh vsvul.r Jr..ar ~ Mrm<wr.l HmpUal, Welfare I,Lnd, ~ New Yrwb (.rt CutrMrRrr) (I. Vr 11U/IAR I A RI IMANN, M I) P.,,f.r thc po.uMr rflr.t uf tnhs..o 1, n,torurr (T, r..r ..I oIr,h.rnr Il.hnrmann MrAn.I In prrrnJlc d1-rJcra, • nrv+ly (7, (.+Ilrar .nJ Il,.+pnat. Phd.drlVh.. rrtnRnr/nl ,)ndramc 1( Pt } i IatANTEF. Anl) IhSTITl/TH)N VI( IOR RI('11AR1)S, M 1), Pro/nsor o/ Sr.rrrry onJ F..erurivr llrwl, l/r. p.ulnvnr of SurMry. StanftMd l/niversity Schod of MeJlcine, San I~rancixo. (xe Ileiter) . R 11 RIObnN, M D„ Pro/rasor of Pa- rllydoRy and Onrr nn, laAorarnry of E.I,rrlmrnlol Pa/holnp, U•iversily of Tesas Medkal Rranch, O•Iveston. SYI)NEY C. RIT'1 FNB)=.RO, Prr D., Pro- /rrs.w of B,M Irri.Jof1'. llnirenily of Southern ('alifornia, 1 os Angeks. BFNSON R. ROP., M I), Aunclarr Pro• /rlsor of SYrRfry. I)f rflnfnf of SYr- Prrf. University of (~lifornia Schod of Medkine, San Francisco. sEN1AMIN A. RUSIN, PN.D., At.lsr- anr Pro/rtsor of Prlrfk NrabA and Prrrrnrirr Mrdkinr, llaylor Uni.ersilr College of Medkine. Houstrm, les•s. (nnw Mana~cr, eiological Products Ik- velopn:ent, W yelh l aboratoaes, Phil- •Jelphi•) FIFNRY I. Rl1SSFK, M D., Consulrant in ('.nJwvanrldnr L)urwr, U. S. Puh- 1k Flealth Serrke Flospilal, •nd Prrsl- drnr, lhe Ruuel foundation, Ine., Slaten Island, N. Y. WII I lAM O. RUSSI?LL, M.D., Parh.do- rtsr in-cAif/, M.1). Anderson (locpilal and Tumor Institute. University of Tecas Medkal C'enter, /(ouston. Pf:'TI:.R F. SAI ISDURY, M D., llrod, Inrrnjirr Trrarmrnr ('rn/rr, Saint -Joseph Ilospital, Rurbanft, ('al. PAUI. D. SAI7MAN, PuD., Arsismnt l'rn/rlun o/ Runl+.misuy, (/nivetsity of Sornhern ('ahfocni. Schod of Med- kine. I os Angeles AI VIN R. S('/tMll)1, Phr 1), 1)irrnr.n af (',rrnvlrnR, lufts l/mrersiry, MeJ (urJ, Mars PRO)EIT llll.F. A nunpar•tive sludy of the elecls of whrrlt •nJ fractional estractM rd clpr- etle smoke and those of known car cinogens on (1) thc cytology and nucle•r I)NA content of epidermis in varicwn strains of mkc and/or (21 the cylology and Nuclear UNA content ol lung and epithelium of the brtxKhul lree of mice and hamsters (C) FRects of tobacco srrlole condemale on the respir•lory tract and other tissues of the duck (C--P) The b•c/erl•1 degradaliors of nicotine and related compounds. 'Ihe objectlve of the poject is Ihe eluchfatiun of the Inlern.edi•ry snc/•bolism of nicotine otidatiow ((: - P) Study of the •ctkrn of negatively charged ions on Irachtolsronchlal cihary action In Ihe human patient ((') An evlulion of Ihc phenomemu+ of lumor growth enhancement as an •ssay fw carcinogens among the rydycyclk hydroearbons and related compnunds (C) EmWional shess (occupation.l ), tobacco eonsumplion and eoronary hearl dii esse PalholOtK-•n•IomK fllkly of cellular ch•nges In human hrnnchr (l') InRuence of nkaine and c•IechcA.mines upon coron•ry vasumotor lunus in normal and •I.Kro.clflotrc dogs (P) The enzymatic mcchani.m fnr the dark Rsalinn of ('Os hy tobacca I( P) Some •sprcls of mino .1 id n.rlahulism in tobacco leaves 1(' P) Study of •Iriludes Ir.warJ, as well as e lenl, /ype and hisrtny uf. Ira,acco snwlinR In a y.nml tdlear p..pula (iun Snnly or VnIK of Ibe Irl.t/.rn rhip- of annling •nJ nrwrauulin• lu (.mily hishrry, type of .rr.wrdary scMuain/, .c.demic Intrrrar, •t..km ic arhirvrmrnt, anJ srKrd Irl.bun ship. charaUenatics ((') ' 55 SJ

n tv .•..~11. "7^ .~.w (:RANTF.F, AP(I) /PICTITIITI(1N ISAA(' S('HOUR, 1)DS, Pwl), 1)Sr, PrnJr„nr of IfnnAnry nnd Enrhryolnrv and /)ren, llnivcrsity of IlFinois (-dkee of Ixntntry, C'hkap. MAIIR!('E S SF(3A1, M D, Clinkal Prnfn,nr of /lfrd.rinr, lufts llniver- siry School of Medicine, Doslon Mass ; llnrrrnr, 1)rparrnrrnt of lnliab(low Thrraoy. Boslon ('i1y Hospital. CAR1. C. Sf1.T7ER, PMD., Rr,rrcA lrl/or in Phy,rcal AnrAropolnPy, Nn- .ard University. Camlxidge, Masa, .nd l)cp•rlmewl of Nutrition, Flar.ard Scl.cxll of Public lleallh, Bostoe. ('IIARI FS F. SFIF.RW(1OU, M 1), A, ,.,ranr Profruor of Radiology. U vtr. lily of Rocheuer School of Me irse and l)cntislry, Rochesler, N. Y. !)AVI[) I SIMONt M 1), In,rnnrnr /n Afrd/rinr and }rflor in ('ardrora,ru• far RrsrorrA, (lnivenily of Cincinnati ('olkge of Medicine and ('ardiac I ah. oralory, ('incinnati C)<neral Ilospil•1. IOIIIS A SO1 OFF, M.!)., Prnlrssor of Cin4a/ Mrdirrnr and Ch/r/, !)r.uinn o/Crdioloe1, Temple Universily Med- kal ('eeln, Phil•detphia. ERNIiCT SONUIIP.IMFR Pr(D, .(uo rfarr Pro/r,rnr of R/nr~irmirrry, State l)nitcni/) ('ollege of Fortshy at Syra cuse University. Syr.cux, N Y. T M SONNI?BORN, Pwn, ni,tin rri,hrd Srrvirr Pro/runr of 7o..lnre. Indiana llniverslty, Bloomington. SAM S(1RUF, Pu 1)., Rr,rarrh An.aiarr, lhe In.lilute for (•scer Rrseuch •nd I •nltn•u 1lrnpil.l Rese•rth Inslnule, t'hlh•ttlphi. I-RIII1RI( K / S1AR1', Put), Pr.rJn r.v n/ N..rrln.•w I1•r..rd S.1-d uf }•,A.Iq 11r.ltt•, l/n.n•n PROJECT TIT1.E Ftistoln*ic changes in thc ot•t, ph•rynle•1 and n.vl linues of e.perimcnlal •nimals subjected to toh•cco .mo\e (C P) ERecls of ciRarclte smnking on lung func• lion in nocmal suhjecls and patients with certun respirato.y disea•e condi- tions 1(* P) Relalionship 1)f eipretle smokinR to chronic (ohstructive) pulnurn•ry em- Phrsema (C) Morphology and smoking in college tr.duaua: a fifteen year fotlow-up studl (P) Nar.ard Johns I/opkins study of h..dy form as related to smoklng, and the precursors of hyperlensiun and to.- on•ry atlety disease In.estigaliow Into the natural hislory of carcinoma of the lung w0h partkular reftrence to the radiographic appear- ance of sucA proceser, the earliest manifestation of eancer on cheat X- raT photograpt,a and the tabulation of the relationship of smoking habits and occupation with the incidence of lung cancer (('-- P) The eRects of chewing tobacco on the c•rdiovascular system of man ((' P) lhe eNetis of pipe smoking and ciRar smoking on Ihe cardiov•scuta, syslem of m•n(C-P) Study of effect of lob•cro .mnking on surfaetanl and on speufk fatty acids The puri/k•lion and structure dttermina• lion of tht ehlorogenie acid isrrmers Checling and estending the Stephano Puramrcirrn lesl for esrcinotcnicilT 1('/ Chemic•1 •nd physical su dirs of ItK fisa..e prWeins involved in chemrt•1 turinn~rntsn I(- P/ I~.Ptrirnrn/el flodir, of r.n.er rdilirrnll • nrv. Ir.hnatw In .rr rl v~rrnus 'Lr.rrn.•.trd h••m 1o6.,ru nur m ult d•r Ia.rnunon of lurn. runu•n I( / 56 / I GRANTF:F. ANI) 1"(STITIITIOPI C. IIAROI D 51 FFFFE, M D, Dirrctnr of 1 aAormn.4s, Methodist Ifospital, Memphis. Tenn. lA( K P. S1RnNC, M D., Assoriarr Profr».w of Pathology, I ouisiana State University School of Medicine. Ne. Orleans. (.ee Ildmast .wd Mc(:i11) MARION B. SU1 ZBERCER, M D., Pro- Irs,or and Chalrrnan. Drp•rtnrrnr of Drrmarolnry and SyPAifdr.Ry, Ne. Yrw& University Belkvue Medical Cen- ter, New Yorl. (retired• sct Redisch) RIIN^iYl 1'AOII/Rl, PN f)., A,srrbrr Profrs,nr of PsyrAolofl, (:raduarr SrMwl of ewinrs, Adnslnisua(ion, Ilarvard (1.itersilT, boslow. CAR(N 1N!? BEDEIL THnMAS, M.1)., Assrtiarr PrnJrssor of Mrdiclnr, Johns /ltpkins University School of Medi- cine, balti/nore, Md. TISSl1E ('(11 Tl1RE ASSO('IA11ON lAMFS F. P. TOMAN. Pw D, Arn+iarr Professor of Physiolnty and Phurnr..rnlnly, ('hkatso Medical School IANF.TTRAVI:l l, M 1), Arrrwwrrr Pro (r.unr of ('6naal Phrnrxnlr•Ry. ( sw nell Ilnivenlty Medical C'ollege, New YorIt. F. I) WARNFR, M 1), Prnfrrrnr nf Pe- tha/.•[y, Sr•le (lniversily of 1uw• ('dIrge of Medrnrne, Iowa (lly, Slltli/ l)S WARRFN, M I/• llurrrnr nf (nA••rnr•nrrr, (omrr Ilrtrarr6 In•n rur., Nt.r InaLnJ I.e•ru..ra /lrnpil.l, Nuston PIt11Jl:/T TIT1.E Studies of c•,tin(tRcnesis in mrce The effects of environmental faclors on devtlnpmenl of •Iherostlcrrnn as re vealed by aulopsy of accident v.ctime In.esfigalion of the eRecl% of tobacco on the human vascular syslem, hased on the fact Ih.t eer/ain tobacco e/lects are due lo •Ilcrgk susceptil.ifily of specific individuah rather than to ohU ptorilr loak prodrrc/s in tobacco arnnle, and that patients with occlusive vascular diseases respond drRerently than healthy smokcss IP) Critical review of htenlure un psychol oI/• of smuking The significance of different indivalu•I pallerns of circulatory respnese to cie. •pelle amo\ing ((:- P) Studies of genelic diQerences hel.etn smoken and nonsmokers ((• _ P) S/udies of psychologic•1 diflerences he Iwten snwkers nd nunsmokcrs as shown by cr.mparisrrn of figure dra. ings (C P) To assisl in eslahlishmo of summer train ing course in Gs+ne cuUure terhniyucs .( l/niversily of ('olondo MeJnal Schnol, 1)enver, ( n1u (('1 Meehanisms of the psy.holropic tRecls of niluline Effects uf nicoline in the r•hbil vnh tsperimental ctwun•ry athettra.lrnnls 1( r/ Palholngk •nalumic sflr.ly uf tellnlar eh•nltes in human hrunrhr 1C I'I Correl•lion of hrunshr.l tprlhrlr•I chanas wi/h cnmparwhlc csunprs in MAeI tNe.rnt • 1•Ylhr•Ir•(n •nal••rnn s1uJy 1(' /•/ Ihe p.nrrn of n.rre.r,.vs rrl r.nu•u.n• nf the tung in rnun• 14 I 11n1r•P,d,r•lnay u( 1•r.n•.n I..ry r•nsrr 57

4 (:RAtYTF.F. ANf) 1"(CTITIrTI()fY RI('IIARh 1. WF('tlS1 ER, M f), Cfinl• cat PAyrfolnlirr, Montehore IIosl+itUl Inslilute of Rcseuch, Pitlsburgh, Pa. RUSSFI L W. WFI I FR, M I) P . fAdo-l in,. .fAdo- lin, Memo.ial Iloyrital ol Chcsln C'otm/y, Wcst ('heslcr, Pa.: Asurtiafr Pro/t)so, of PorAololy, Hahncnunn Medical ('olktte, 1'hYadelphia. SIMON Ff. WFNhFR, Prt n., Rriea,rA Pro/essne of CAtnsisfry. Uni.errhy of ()1llahorna, No.m... I)(IANF 0 WI N7F1, PN [). P.n/rnor o/ Pharmx(sl.+R., School of Pharmacy, llnivetsily of K.nsa, 1 arrence FRFI)FRt('K 1?. WIIISKIN, M 1). (' M, f)irrclor, [hridnn of //rabA .nd Pe.e sonaluy F.~rdrArfun, lhe Age ('rnter of Ne. Enlland, lnc , 6oston. (see I )ihncr ) R(X)FR / WII l IAMS. Prr D., Prn/ti)oe of CArmhrry and Dbnrn., ( farfon Forndafion SwrAemkal fnllurrr, t he (Iniverslly o( Tesas, Austin. 1. F.1)WIN W(X)1), M D., Prolrnnr of RCrdkMt, Medkal Col/ege of Geagia, Augusl• S(IMNFR W(X)h, la, Mt). Au/hfnnt rco/runr o/ ParA,d.rty, l)rporr.nrnf a/ Pod<doly, Johns Iloqlins Oniversily, hallimore, Md IOIIN P. WYA1 I, M l), l.o/rrr.r of ParArJoRr, t1 I oois Oni.ersily School of Med..ine, St I ouis, Mo PR()JF'(T TITT.F. ERect of cilaretlc smolin/t (m cerehral blood flo., cercbral mctahuham. Mootl pses, h1ooA pl(, arterial prda Pres. surc curves, ekclrocardrolrams, anJ electrnence)+halolrams ll') Pathologic natomic slaly of cellular chanRes in human hrnnchi 1(') A scletled, eslenJeJ anJ tkLileJ study of human bronchial mu.osa A aualitalive and uanlilative study of the individual ~yPhenol conlenl of ciprelte Iobacco and the smole and 'tari resultint from citflrelle smoking and Iso rqudy of the (ale of Ihese com(r)unds in the animal rc.piralo.y sy+lem (C P) The iJentrfkaliow trf lnJiv.Aual prlyphe. nolrc compounds preAcnt in the smoke from cilarelles and Ihe' Preparalion, .herc feasiblc, of these lF.mpounds fcw studies ow thcir metahtdism and Possibk tlfecl on human he3llh The delermination of the chronic eflecls of orally aJm/nistfrtJ nKlMine tMl serum choleslerol anJ pl.csphrJipiJs; the cJrclrrrudiotraphic resry.n.e Irs erRru.nvint; and the v:r.culat PalhntoRy of tfnJc.terul fed rahhi/. Il, P/ A.ludy of central aclu.n% of nicrHine Pilot stnJy of sm.Aing hahits of ARe ( enter memhcrs (C P/ An in.e.liRalion of hiochcmiial factors associmed with susceplrhihty to lung Cancer The eRect of prolonged inhalatinn of to- bacco smole .nd of (~rrdonReJ abslin• encc (rnen Ihe ux ol Irrharcn on the t ipheral rascular re.IwrnM 1u acure halaliun of lobacco .mnlc rn man lC P/ Fvaluatwtn of e1ldoRk /atlors, such as otcupa)i.mal hauarJs and hahits, an ParhoioRic peeulrarila, in the hrnt term surri.al of Pal.rnlr /rdlurinl re KtInM fM brnnlholenN eartrMlma An Invr.liRalitrr inlo the natlue of the PiRmentary ksluns in trnlydobulat empbysema (C) 59 i

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