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CURRICL'LUMI VITAE OF GORDON SATO Title: Professor of Biology Address: Dept. of Biology, Q-058 303 Warren Campus University of California, San Diego La Jolla, California 92093 (714) 452-2545 Educational Background: B.A. degree conferred in 1951 in Biochemistry at University of,Southern California Ph.D. degree conferred in 1955 in Biophysics at Cali,=:,rnia Institute of Technology 'Chronoloyy of professional appcin:.^ents; 3/75-present: Professor, Dept. of Biology, University of California , San Diego, La Jolla 6/74-3/75: Sabbatical Leave, Visiting Professor, Basel Instit;:te of Irrmunology, Basel, Switzerland 1959-1974: Professor, Dept. of Biology, University of California, San 1968-1969: Diego, La Jolla- Professor, Graduate Dept. of Biochemistry, Brandeis l;niv., 1963-1968: Waltham, faass. Assoc. Professor, Graduate Dept. of Biochemistry, 6rande:s 1958-1963: Univ., b1a1 tham, Asst. Professor, Mass. Graduate Dept. of Biochemistry, Era.r.~~eis Univ., Waltha.^i, Mass. 1956-1958: Instructor, Molecular Genetics, Univ. of Colorado I'ec.icai 1956: School with T. T. Puck Jr. Research Virologist, Univ. of California with Cun:ner 1953-1955: Stent Teaching Assistant, Microbiology, California Institute o= Technology with Max Delbruck Membership in Professional Societies: Sigma Xi, 1955 Endocrine Society American Societies of Irrarunologists Tissue Culture Association Insternational Cell Research Organization American Society of Biological Chemists American Association for Cancer Research American Academy of Arts and Sciences = FXHIBIT € = z Servi ce or. Advi sory Corr.ni ttees : Study Se:.tien Panel t•tember (t•,olecular Biology ) NIH 1968-1973 Breast C.an~:er Task Force NIH 1970-1975 !'SF ccr.soltant Californie Cancer Researcr. Ccmnittee Cancer S,ecial Program Aovisory Cocrmittee NIfI 1980 C `t°R ~N 0 11162

2 Gordon H. Sato Research Interests: Establishment of human tumors as transplantable lines in nJ mice Establishment of uman tumor lines in cell culture Definition of the hormonal reouirements of these lines in the absence of serum Purification of peptide orowtn factors (hormones) from blood cpeai anc ttissu~ extracts Treatment of human cancers with hormone analogues in culture and in r;;ce Alice EJ_ec ~260 CTR HN 0 11183

GORDON SATO PUBL I CAT I ONS 1. Sato, G.H. Activation of cofactor requiring bacteriphaae by urea. Science, 123: 821 (1956) 2. Fisher, N., Puck, T.T., and Sato, G.H. Molecular crcwth reauire^~ents c.` single martnalian cells. Proc. Nat. Acad. Sci., 42: 900 (1956) 3. Sato, G.H., Fisher, N., and Puck, T.T. Molecular growth require^-ents of single manrnalian cells, Science 126: 961 (1957) 4. Stent, G.H., Sato, G.H. and Jerne, N.J. Dispersal of parental nucleic acid of bacteriophage T4 among its progeny. J. Mol. Biol. 1: 134 (lc":l 5. Fisher, H., Puck, T.T. and Sato, G.H. Molecular 5rowth requiremen_-S c= single man.malian cells. J. Exp. Med. 109: 649 (1959) 6. Sato, G., Zaroff, L. ar,c Mills, S.E. Tissue culture populations and relation to the tissue of origin. Proc. Nat. Acad. Sci. 46: 96-1 (i9-E;;; 7. Zaroff, L., Sato, G. and Mills, S.E. Single cell platings frc:-. =res~1;• isolated ma -~malian tissue. Exp. Cell Res. 23: 565 (1961) 8. Buonassisi, V., Sato, G. and Cohen, A.I. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Nat. Acad. Sci. 48: lla= (1~~2) 9. Sato, G. and Buonassisi, V. Hormone-secreting cultures of endocrine t::-^r origin. Nat. Cancer insti. ~donograph No. 13, p. 81 (1964) v 10. Stollar, V., Buonassisi, V. and Sato, G. Studies on hormone secre:ir.c adrenocortical tumor in tissue culture. Exp. Cell Res. 35: 608 (195~` 11. Maloof, F., Sato, G. anc Soodak, M. Dissociation of iodination frc-: :~rc.:in synthesis in the rat tnyreid. Medicine 43: 375 (1964) 12. Sato, G. and Buonassisi, V. Hormone synthesis in dispersed cell cultures. Retention of functional differentiation in cultured cells. Wistar ir.s:i- tute h:onograph No. 1, Philadelphia, p. 27 (1964) 13. Sato, G. Use of dispersed animal cell cultures in the study of specialized physiologic processes, in "Methods in Medical Research", Vol. 10, H.N. Eisen, Editor-in-Chief, Chicago Year Book Medical Publishers, Inc. p. 323 (1964) 14. Humphreys, S.R., Sato, G and Goldin, A.I. Transplantation characteristics and response to chemotherapy of a nurine adrenal tumor. Europ. J. Cancer 1: 125 (1965) 15. Sato, G.H., P.ossman, T., Edelstein, L., Holmes, S. and Buonassisi, V. Phenotypic alterations in aarenal tumor cultures. Science 145: 3673, p. 1733 (1965) CTR HN 011164

Gordon Sato Publications (continued) 16. Yasumura, Y., Buonassisi, V., and Sato, G. Clonal analysis of differen- tiated function in animal cell cultures, I. Possible correlate:' rein- tenance of differentiated function and the diploid karyotype. Cancer z:-_s. 26: 529 (1966). 17. Sato, G. H. and Yasumura, Y. Retention of differentiated functicn in dispersed cell culture. Transactions of the New York Academy of Scierces, Ser. 11 28: 8, p. 1063 (1966). 18. Yasumura, Y., Tashjian, A. H., Jr., and Sato, G. H. Establishr:ent cf `c;;r functional, clonal strains of animal cells in culture. Science l5<: p. 1186 (1966). 19. t,chi', B. and Sato, G. !'. Irproved in vi tro survival of no+-Tal spleen cells. Science 157: 3787, p 449 (1967). 20. Shin, S., Yasurura, Y. and Sato, G. H. Studies on interstitial cells ir tissue culture, II. Stercid biosynthesis by a clonal line of rat ;estic::~ar interstitial cells. Endocrinology 82: 614 (1968). 21. Cuprak, L. J. and Sato, G. H. -Nutritional requirements of mouse adrenal 22. cortex tumor cells in culture. Cell Res. 52: 632 (1968). Schir:-:er, B. P., Ueda, K. and Sato, G. H. Site of action of adrenoccrt'- cotroohic hormone (ACTH) in acrenal cell cultures. Biocnem, oiea r::s. =.~_. Comm. 32: 5 (1968). 23. Benda, P. , Lightbody, J. , Sato, G. , Levine, L. and S,.-~eet, t•,. Di f-:ed rat glial cell strain in .issue culture. Science 161: 3c3°, 24. Tashjian, A. H., Jr., YasuTura, Y., Levine, L., Sato, G. H. and car?:er, ,'. L Establishr-ent of clonal strains of rat pituitary tumor cells tnat s_cre:= growth hormone. Endocrincio7y 82: 342 (1958). 25. Augusti-Tocco, G. and Satc, G. Establishment of functional clcnai lines " neurons from mouse neuroDlastoma. Froc. t+at. Acad. Sci. 64: 311 (i96:). 26. Rosenthal, 11. D., Wi shnow, R. and Sato, G. H. The in vi tro crcwth and d' •- ferentiation of clonal pe::ulations of rultipotential r.-,ouse cells derive= from a transplantable testicular teratocarcinoma. J. Nat. Cancer Inst. 44: 1001 (1970). 27. Pfeiffer, S. E., Herschmar,, H. R., Lightbody, J. and Sato, G. Synthesis '-y clonal line of rat glial cells of a protein unique to the nervous syste-. J. Cell. Physiol. 75: (1:7r). 28. Posner, M., Gartland, U. .:., Clar:, J. L. , Sato, G. and Hirscn, C. A. Development of hormone resocnsive ceil strains in vitro. Svrcosium cf :'° Society for Developmen :.ai 5icica., Suc;. 4, p. 114 1970). 29. Sa*.o, G. , A;;ousti-Tocco, G. ,"rosner, t':. Por'rone-secretine anc her-cnG-- ponsive cell cultures. Recent Prearess in hormone Res. 26: 53a C.. ! R E [ E `4 t.,f 111855

Gordon Sato Publications (continued) 30. Auousti-Tocco, G., Sato, G. H., Claude, P. and Potter, D. D. Clonal cel' lines ). of neurons. International Society for Cell Biology Symposia 9: 1C;9 ;1_7 D 31. Sato, G., Clark, J., Posner, M., Leffert, H., Paul, D., Morgan, M. anc CciC. Enrichment culture techniques for selection of cell culture strains ::... desirable physiological and genetic properties. In: Karolins,:a S"-^c-~_ ~ Research Ftzthods in P.eorccuctive Endocrinoloov, 3rd Sv^:ocs~uM: : -_ Methods in Reoroauctive Cell Bioloev, Geneva 1971. (E. Dicz.aiusy, ec.; Periodica Publisners, Cc~er.naoen. P. 126 1971 . 32. Pfeiffer, S. E., Herschcan, H. R., Llghtbody, J. E., Sato, G. and Modification of cell sur'ace antioenicity as a function of c;:lt::r: .•.~. ,. J. Cell. Physiol., 78: 1415 (1971). 33. Posner, M., Nove, J. and Sato, G. Selection procedures for ra-:;aiia^ ce:is in monolayer culture. In Vitro 6: 253 (1971). 34. Shin, Seung-Il and Satc, G. Inhibition by actino-•ycin D. cyclcnexi-ic_ ar.t puromycin of steroid synthesis induced by cyclic A,'.,P in interstitial cells. Biochem. and Biophys. Kes. Co.~. 45: 501 (1971). 35. Clark, J. L., Jones, K. L., Gospodarowicz, D. and Sato, G. H. Gro-.:.h resconse to hormones by a new ra: ovary cell line. Nature New Biol. 236: ic3 1972). 36. Schrccder, F., Sato, G. and Gittes, R. Human prcstatic adenocarc•..c-•e. Gr.,.:th in monolayer tissue cul.jre. J. of Urology 106: 734 (1971). 37. Sato, G. Hor^~one deoenc_n: animal cell strains ?^ cul*_ure. In: ~^1;; Gnnnti a-, nnVa10: ~'C1CCV. The Society 0' Ge,n,ora Symposiu-,:, nocos roie, '•'.assacnusetts. (r•'. Sussman, ed.) P. 475 (13 7" 38. Paul, D., Leffert, H., Sato, G. and Holley, P,. W. Stimulation cf CNS ard protein synthesis in fetal liver cells by serum from partially heca.ec.c~iz°_' rats. Proc. Natl. Acad. Sic. 69: 374 (1972). 39. Barley, F. W., Sato, G. H. and ABeles, R. H. An effect of vi-za~in 5,: ceficiency in tissue culture. J. Biol. Chem. 247: 4270 (1972). 40. Gardner, D. A., Sato, G. H. and Kaplan, N. 0. Pyridine nucleotides in normal and nicotinamide depleted ad,•enal tumor cell cultures. Developmental Eioicgy 28: 84 (1972). 41. Gospodaro',iicz, D., Jones, K. L. and Sato, G. Purification of an ovarian cro-:;th factor (CGF) present .. :ne pituitary gland. Endocrine Society: 55th Meetings: Abs. =347, p. A-222 (1973). 42. Yonis, A. A., Schultz, . and Sato, G. H. The secretion of flbrin3l_v;•-. cultured rat ovarian t.:-7or cells. Biochem. Biopnys. Res. Ce-~r^. -- 43. P.uddle, R. H., Nelson, . G. and Satc, G. "Introduction" N'1 renber~ Sato, G. ".:euroolastc-a Cell Karyotypes'. In: Contric icns c` : Svste:,is to t+eurooioloc:'. NR? Gulltein, Vol. 11, No. :, (1-:-, 73). 4C. Sato. G., :ditor. Tissue Culture ef the t:Frvous System. Unl. 1. Pler:, CTR HN 0 11186

45. Armelin, H. A. and Sato, G. Cell cultures as model systems for tre s:.;d" of growth control. World Symposium on Carcinogenesis, Jonns Hopl:ins University Press (1973). _ 46. Sato, G. The establishment of hormone-dependent cell lines. In: Me:ncts cf Enzvr:oloov. (Kaplan and Coldwick, eds.) Vol. 32, Part B: 557 (197ti). ' 47. Armelin, H. A., Nishikawa, K. and Sato, G. H. Control of ma,--alian c_l1 cro-;:th in culture: An action of protein and steroid hormones as effec:or su:~s:ances. Iq: Control of Proliferation and Animal Cells. (Clarkson and Baserga, ed.) Cold Spring Harbor Puolisners (1974). .48. Gospodarowicz, D., Jones, K. and Sato, G. Purification of an ovar:an factor for ovarian cells from bovine pituitary cells. Prcc. Na:i. ;'c:.z. Sci. 71: 2295 (1974-75). 49. Nishikawa, K., Armelin, H. A. and Sato, G. Control of ovarian cell crc.::~ in culture by serum and pituitary factors. Proc. tJatl. Acad. Sci. USA 7.: 483 (1975). - 50. Sato, G., Desmond, W. and Kelly, F. Human prostatic tumors in ccnditicred ar.ir,als and culture. Cancer Che-othe-rapy Reports 59: 47 (1975). 51. Sato, Gordon H. The role of serum in cell culture. In: Bioche-ica'. ~n of Hormones, Vol. III. P. 391. Academic Press, Inc., Ne.: York, San =,-;,n;-'sco London 1y75). 52. Stiles, C. 0., Desmond, i:., Sato, G. and Saier, Jr., M. H. Failure of 'j-an cells transformed by Simian Virus 40 to form tumors in atnyTic nuee Proc. Natl. Acad. Sci. USA 72: 497 (1975). 53. Hayashi, I. and Sato, G. Replacement of serum by hormones permi.s .__, tt, of cells in a defined mediu:^. Nature 259• 132 (1976). 54. Desmond, k'., Wolbers, S. and Sato, G. Cloned cell lines frc- mouse --z--'r_: tumor requiring andreger. ~cr growth. Cell 8: 79 (1976). 55. Stiles, C. D., Desmond, W., Chuman, L. M., Sato, G. and Saier, Jr., M. H. Growth control of heterologous tissue culture cells in congenitally a:^;r-:ic nude mice. Cancer Research 36: 1353 (1976). 56. Stiles, C. D., Desmond, ~., Chuman, L. M., Sato, G. and Saier, Jr., M. H. Relationship of cell groaath behavior ir, vitre to tumorigenicity in atnv^ic nude mice. Cancer Research 36: 3300 (1976). 57. Havashi, I. and Sato, G. Feolacement of serum by hormones in cell 58. media. Arch. Biol. Med. 10: 120 (1976). Sato, G. Growth factors and cellular proliferation I & II. Third Tissue ~ Culture Assoc., Inc. Decernial Review Conference: Cell, Tissue anc Gr__^ ;-, Culture, Lake Placid, t;.Y., Sept. 13-17, 1976. Mcno;; r&ph. In: National Cancer ~ 59. Stiles, C. C., Roberts, P. _., Saier, M. H. and Sato, G. Growth rec;', _'cr and suppression of metastasis in the congenitally athymic nude mouse. n. 1•'.nAnrn i rorr'.c i n CTR MN 01118-

Sato, 3;rdcn H. 401-LC-.73uJ Gordon H. Sato publications (continued) 60. Sato, G. Growth of HeLa cells in serum-free hormone suoplemented In: Advances in Patholoov =6. Cancer Bioloav I;'. DifferPntiati:n e-- Carcinooenesis. (Gorex, renoglio, r,ind, ed.) Stracton intercont;ne•::=i Medical Book Corp., ;iew York. p.227 (1977). • 61. Reid, L., Holland, J., Jones, C., Wolf, B., Niwayama, G., Villia,:s, Kaplan, N. and Sato, G. Some of the variables affecting the suzcess c- ' transplantation of human tumors into the athymic nude rouse. :n: of the Syroosium on the Use of Athvmic P1ude) Mice in Canccr Resear-~~. Houchens and A. Uverjera, eas. Gustav Fischer I;ew York, inc. ii:7E;. 62. Reid, L., Colbern, P., Sato, G. and Kaplan, N. Approaches to Cne7otn_-.:.2v usina the nude mouse. In: Proceedincs cf the S-r:oosiu:7 or the ~'s_ - (Nuae) tiic°_ in Cancer Pesearcn. (li. Houcnens arc ~. ilver~cra Fiscner (;e;i York, inc. (1772). 63. Rizzino, A. and Sato, G. Growth of embryonal carcinoma cells irn ser::--'ree medium. Proc. Natl. Acad. Sci. USA 75: 1844 (1978). 64. Mather, J. and Sato, G. Hormones and growth factors in cel l cul .:;re : PrcI-le-:s and perspective. In: Cell Tissue and Ornan Cultures in .i~urc~ic'.c^:, (Federoff, ed.) Academic Press. pp. 619 (1978). 65. Hayashi, I., Larner, J. and Sato, G. Horrr,onal gro-:rth control of ce'.?s in - culture. In Vitro 14:23 (1978). 66. Hutchinas, S. E. and Sato, G.H. Grot•rth and maintenance of He'_a cells serum-free medium sucole.i.ented with hormones. Proc. Natl . :,cad . __i. 75: 901 (1978). 67. hu, R. and Sato, ''.'.. Pe-la.r.eT.ent of serum in cell cul Jre ty r,c., .,,.__. *. study of hormonal reculation of cell grc.:th and speci'c cOnO Ex_-.__.,-_,. Jrnl. of Toxicology d:nviror,m2ntal Health 4: 427 (1973). 68. Sato, G. and Reid, L. The reolaceT,ent of serum in cell culture bv ._ .snes. In: Bicche:-istrv ar.d ^de of Action of Nor:-ones i' Interna;icr, l of Biochemistry, Voi. 23 (::..'. ricKenoerg, ea.) University Park Press, Baltimore, ttaryland pp. 219-251 (1978). 69. Sato, G., Hayashi, I., Hutchings, S. and Mather, J. Replacement of serum by hormones and its implication for primary cell cultures. Tne international Congress Series No. 408, Pancreatic Beta Cell Culture. Proc. of tne Fiftn Workshops Conf. Hoechest, Kitzbunel. 5-9 Oct. 1976 (eds. E von tJasielewski and ~ W.X. Chuck) Excerpta Nec'ica, A,msterdam-Cxford (1978). 70. Gottenstein, J.E. and Satc, G.H. Grovlth of a rat neuro:iastc-.a cell 'ne seru7,-free supplementec r:ediur.;. Prcc. <<atl. Acad. Sci. (i;SA) __1- ;l?7=;. ~ 71. Bottenstein, J., Havashi, :., r:utchings, S., Ptasui, "., ';atner, J . , U.E. , Ohasa, S. , 2izzinc, n. , Sato, G. , Serrero, G. , ':ol:`e, R. an~ The gro.vth of cells in serum-i`ree ncrnone supaie77cntea me_;at in. _ i re (A Volu,~.e of t-letnods ir :nzv-olnov) Vol. 58. po. 9»-1G9. ('.. . Ja.. __ ar.c I.H. Past3r,, eas.) ,cacie.:,ic rress, Neu York. (1:i:) . 72. Barnes, D. l•l. and Sato, G. H. Gro%•:th of a human breast cancer cell ,.-? l' 7! CT 1 "Y \ 1N 0 11166

Gordon H. Sato publications (continued) 73. Mather, J. and Sato, G. Grc:rth of mouse melanoma cells in hormone-s:c-;e-e~tcd serum-free medium. Exp. Cell Res. 120: 191 (1979). 74. Orly, J. and Sato, G. Fibronectin mediates cytokinesis and gro::th of rat-foliicular cells in serum-free medium. Cell 17: 295-305 (1979) 75. Taub, t•f. , Chuman, L. , Saier, Jr. , M. H. and Sato, G. The gro::th of epithelial cell line (PtOCK) in hormone-supplemented, serum-free medium. ?rcc. , Natl. Acad. Sci: (USA) 76: 3338-3342 (1979). . 76. Bottenstein, J. E., Sato, G. H. and Mather, J. P. Gro,•rth of neuroepi:^e'ial- derived cell lines in seru^•.-free, hormone-supplemented media. I,+: Hcr-cr_s and Cell Culture, Book B. Cold Spring Harbor Conferences on Cell Prolifera:.0r, Vol. 6 Co d Spring Haroor Laboratory, 1979. 77. Serrero, G. R., McClure, D. B. and Sato, G. H. Gro,-ith of mouse 3T3 fi5rctiasts in serum-free, hormone-su;,;lemented media. IN: Hormones and Cell Book B. Cold Spring Harbor Conferences on Cell Proliferation, vol. c. C:.:: Spring Harbor Laboratory, 1979. 78. Ross, R. and Sato, G. Cell culture and endocrinology: An overvie:r. .•.. 79. Hormones and Cell Culture, Book B. Cold Spring Harbor Conferences on Ceil Proliferation, '1o1. 6. Cold Spring Harbor Laboratory, 1979. Barnes, D. and Sato, G. Growth of a human mamnary tumour cell line in a serum-free medium. Nature 281: 388-389 (1979). 30: t,ather, J. P. and Sato, G. H. The use of hor:rone-supple:^ented serum-free -ec'i a in primary cultures. Exp. Cell Res. 124: 215-221 (1979). 81. Rizzino, A., Rizzino, H. ar.d Sato, G. Defined media and the deter:7-ina:icn of nutritional and hormonal recuire^:ents of mamnalian cells in culture. P.ev i eY:s, Vol . 37: 359-37_0 (1979). 82. Tauh, M. and Sato, G. Gra,•rth of kidney spithelial cells in horTene-suc~ _-en_es' serum-free medium. Jrnl. of Sua raTolecular Structure 11: 207-216 83. Sato, G. H. Towards an endecrine physiology of human cancer. First :n.ernational Congress on Hon;.ones and Cancer and E1,60 Worksnop on Specific Grc.rtn - Rome. October, 1979 84. Sato, G. H. Cell culture, hor^;ones and gr(Y.ith factors. First International Congress on Hormones and Cancer and Eh:GO Workshop on Specific Growth F act:rs, Rome. October, 1979. 85. Bottenstein, J. E., Skaper, S. D., Varon, S. S. and Sato, G. H. Selective survival of neurons from chick embryo sensory ganglionic dissociates utili'-ing serum-free supplemented medium. Exp. Cell Res. 125: 183-190 (1980). 86. Barnes, D. and Sato, G. Pte:neds for gro•:rth of cultured cells in seru,--fr_= medium. Anal. Biochem. 102: 2-;-;-270 (19S0). ` J7. cn Mather, J., Wu, R. and Sato, G. Role of hor^:on2s in the cros•rth anc re_Qu:__- of cells in serum-free.medium. ;N: Gro::th Recuire!7ents of !'ertebrate '' in vitro. Ca,nbridge University Press (in pr:ss). 8EE . Ohasa, S., Sato, G. and ttasui, H. Developr•ent of a serum-free r-ediu^ su-.:',erentec with hor„ones for the growth of a normal rat ovarian cell. (Suomitted for cuc.) CTR MIN 0 11169

. G~rdon H. Sato publications (continued) 89. Bottenstein, J. E. and Sato, G. H. Fibronectin and polylysine requiremer.t for proliferation of neurcblastama cells in defined medium. (Submmitted, C=i'. 1979). 90. Rockwell, G. A., Sato, G. H. and McClure, D. B. The growth requirements e` S:'40 virus transformed Balb/c-3T3 cells in serum-free monolayer culture. (Su--.-itted). 91; Darr:on,* t•1., Bottenstein, J. and Sato, G. Differentiation of a teratccarcir.c.;,a cell line in a defined medium. (Submitted, Cell). 92. Taub, M. and Sato, G. H. Growth of functional primary cultures of kicr..ey epithelial cells in defined medium. (Submitted J. Cell Phys, 197-0). 93. Dray, F., Bottenstein, J. and Sato, G. H. Prostaglandin D2 is a potent inhibitor of growth of rat neuroblastorna cells (Submitted Nature 1979). 94. Orly, J., Erickson, G. F. and Sato, G. H. Serum suppresses the exoressi`- cr hormonally-induced funr ions in cultured granulosa cells. (Submi:.ed C_.. 1ScD). 95. IlurakaTi, H., Masui, H. and Sato, G. H. Gro-ath of mouse myel oma cel 1: serum-free medium: A systematic procedure for replacement of seru-n :.it-. _efir,ed graath factors. (Submitted to Anal. Biochem, 1920). 96. Wolfe, R. A., Sato, G. H. and McClure, D. B. Requirements for th~- contir.ucus serum-free culture of rat C6 glioma. (Submitted J. Cell piol., 19c0). 97. Sato, G. H., Hayashi, I. and Nakamura, T. On replacing serum with hc^-.Or=s in - cell culture media. (In preparation.) 98. McClure, D., Ohasa, S. and Sato, G. H. Discovery of a nev, grc.rt^ ;ac.cr in rat submaxillary gland. (In preparation.) 99. Wolfe, R., au, R. and G. H. EGF-induced down regulation does ,.... .,_...:r in serum-free medium. (In ;.re_~aration. ) 100. Orly, J., Erickson, G. F. and Sato. G. H. Growth and diflferentiatic•n c~ :~rimary rat granulosa cells in seru--free medium. (In preparation) 101. Darron, P1., Bottenstein, 'L and Sato, G. Neural differentia~ior, culture of embryonal carcinoma cel is in a serum-free defined mediumi. (Sucmittec Press). 102. Murakami, H., Masui, H. and Sato, G. Growth of hvbridoma cells in ser,:-free medium: Identification of ethanolamine as a novel growth factor. (In prep.) 103. Barnes, D. and Sato, G. Factors which stimulate proliferation of breast cancer cells in vitro in serum-free medium. IN: Systematics of Marrrnar4 Cell Transfcr- mation_TEd. McGrath, C.) Academic Press Inc., N.Y. (in press). k19c0) 104. Barnes, D. ,!~cClure, D., Wolfe, R. and Sato, G. A seru-, factcr influencin; spreading of ce'is in serumn-free medium- J. Supra^oi. Str;,_..,.: (in pre:s) (1980). 5. Serrero, G., Darnon, ~•.., Darnes, D., Rizzino, A. erd Sato, G. E*fect ., seruT factor on attachir,ent and nro~.atn of emcryona: carcir,,:r:a cells in culturc. (Sucmitted to In Vitro) (14c0). CTR MN 011190