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.i: l prrionnd Ii:Kd on p.7s 7• bhinni-j wth M+s?nrnci,~ vi Invyti~ror. (h- con ::-:,r.... :-.y> s.~d lotlow tlff L.&ms ionwr for e,.:h perscr-I a1RTH0:.TE/:,ta,rr.1 S0'Ua •;: ..r .. , RVSMT:..u tt . TL%.CE OF BIRTM St.:e. Counrry/ Madison, Ylisco'lsir) Professor of Oncology ?~_S_::T N:.TION4aLITY ll1non-U.S eitaen, ind.c&^f iind of via and r+pirstion d.trl U.S.A. E O V G+ i I 2'1 !9-n..;tn Dsrj/wr..rt. Cnining.nd includf poatdcctorjt/ SEX ?lovenber 24, 19. Q t.l339 0 Flm41% INSTITUTION AND LOGIT/ON DEGREE ~'cAR CnVFERREO SGE!:TIF IC FIELD Beloit Coilege, Beloit, Wisconsin B.S. 1939 Chemistry , University of W±pconsin, Madison M.S. 1941 Biochemistry University of Wisconsin, Madison Ph.D. 1944 Biochemistry Biochem. Dept., University of Wisconsin Fellow 1944-1945 Ncr:OIRs Clowes Awa:d, A.~aerican Association for Cancer Research, 1979 ?A:.,0.4 ri:SEAFtCr1 INTEREST Bioc'ie:ais::y of Ca:cinoge^esis R:ScAriCH SIr,'PORT lS++ih rxnanrl See Next ?age ROLE IN PROPOSED PROJECT Co-Investigator R25cA?C-~ TR0=:bSfO'+AL;XP;?I:'.Cc ..;M pi.%s.rntCo=it~on,(%:•••r:n:n2 ands.prrix+c. r.rle+anttoa.r.olp•oi-<c Li:1 a erenytryrrvnrjDrrpu!)tLrhon{ Donota.ee+dloreKhind.rid.aal.l PCS__.~^S ~~ta: - ?:cfessor cf C^ce'-o;_•, ,,..ive:sity of ;7isconsin 1967-prese^_ .;sscc:ate ?:c:essor c° Cn-ology, Uni versity of Wisconsin 1954-67 Ascistant ?:ofessor of Or.co'_ogy, Uni versity of Wisconsin '1949-54 Ir.s::.._to: c; 0-colc5: ,:,'...versi*yo o f Wisconsin 1945-49 P:o°_essicn?1 activities: Ca.tc_ ial Advisorv_ Boa:~, C:i:CS? RES EARCH 1959-64 Associate Editor, CANCER ?ESSARC.'. 1973-80 ~dviso:y C=Mittee on Pat^.ogenesis o C4r.cer Societv f Cancer, Ftimerican 1960-63 4,z!':13ory Corranittee on .ns.__j_icna1 Research Grants, 1967-74 %t'erican Cancer Soc.e:y; nssista.^.t Chai:^.an, 1971; C:-_..^3n, 1972-74 ^ C _ o ^ "' , a G: __? .. _ne L..n :. _er a:3. Fo:- , "3t:o:;a1 Cancer Inst. 1968-73 Cancer Segr:er:.., ':---..na_ Cancer Inst 1971-74 ` ?:..tec..en Cc.•_.,.-_ee, Researc:i Councii .971-74 (continued on next page) r~r ~~{ ttL T Pr! ))~1 = EXHIBIT : ~- I r 3/s1 . c . t.N••.'v !.T i rcr.-r. or.Y• .... r-- CTR f i i i 0.i 0"r 2.L

Co;-,mittee on Non-nutritive of Sciences Sweeteners, National Academy 1972-75 Experimental Therapeutics Study Section, NIH 1974-76 Subcommittee on Arsenic of the Committee on Medical and Biological Effects on Environmental Pollutants, National Research Council 1974-75 Committee for the Review of Data on Carcinogenicity of Cyclamates, NCI 1975-76 CZ:ci..c- ,^~^.,..e~~--° -^'_en:i=ic Advisory Committee, NCI - -_ 1977-80 Council for Research and Clinical Investigative Awards, American Cancer Society 1977-80 Board of Directors, Am. Assoc. Cancer Research 1979-82 Publications, 1974-early 1980 1974 Boutwell, R. K. The Function and Mechanism of Promoters of Carcinogenesis. CRC Crit. Rev. Toxicol., 2: 419-443, 1974. Bowden, G. T., and Boutwell, R. K. Studies on the Role of Stimulated Eoideraal DNA Sy-athesis in the Initiation of Skin Tumors in Mice by N-Methyl-N'-nitro- N-nitrosoguanidine. Cancer Res., 34: 1552-1563, 1974. Bowden, G. T., and Boutwell, R. K. The Replication of Mouse Skin Epidermal DNA to Which is Bound 7,12-Di.-.zthylbenz(a)anthracene. Chem.-Biol. Interact., 9: 15-23, 1974. Bowden, G. T., Shaoas, B. G., and Boutwell, R. K. The Binding of 7,12-Dimethylbenz- [a)anth:acene to Replicat:r.g and Non-replicating DNA in Mouse Skin. Chem.- Bio1. Interact., 8: 379-394, 1974. Bowden, G. T., Slaga, T. J., Shapas, B. G., and Boutwell, R. K. The Role of Aryl Hydrocarbon Hydroxylase in Skin Tu.:or Initiation by 7,12-Dimet3hylbenz[a)anth:acene and 1,2,5,6-Dibenzant::racene Using DNA Binding and Thymidine- H Incorporation into DNA as Criteria. Cancer Res., 34: 2634-2642, 1974. Helrnes, C. T., Hillesund, T., and Boutwell, R. K. The Binding of Tritium-labeled Phorbol Esters to the Macromolecular Constituents of Mouse Epidermis. Cancer Res., 34: 1360-1365, 1974. Slaga, T. J., Bowden, G. T., Shapas, B. G., and Boutwell, R. K. tdacromolecular Syntnesis following a Single Application of Polycyclic Hydrocarbons Used as :nitiators of Mouse Skir. Tumorigenesis. Cancer Res., 34: 771-777, 1974. Slaga, T. J., Bowden, G. T., Scribner, J. D., and Boutwell, R. i:. Dose-Response Studies on the Abilit'; of 7,12-Dir.et,`,y:benz[a)anthracene and Benz(ajantr:rnce^^ to Initiate Skin Tu:*o:s. J. Nat:. Cancer Inst., 53: 1337-1340, 1974. CTR HN 010922

1975 Bowden, G. T., Trosko, J. E., Shapas, B. G., and Boutwell, R. K. Excision of Pyrimidine Diners from Epidermal DNA and Nonsemiconservative Epidermal DNA Synthesis Following Ultraviolet Irradiation of Mouse Skin. Cancer Res., , 35: 3599-3607, 1975. O'Brien, T. G., Simsiman, R. C., and Boutwell, R. K. Induction of Polyamine-bios- ;^•_!:et'__ Z.-.=;M:S in Mc_se Epidermis by Tumor-promoting Agents. Cancer Res., 35: 1662-1670, 1975. O'Brien, T. G., Simsiman, R. C., and Boutwell, R. K. Induction of the Polyamine- bios•ynthetic Enzymes in Mouse Epidermis and Their Specificity for Tumor Promotio: Cancer Res., 35: 2426-2433, 1975. Slaga, T. J., Bowden, G. T., and Boutwell, R. K. Acetic Acid, a Potent Stimulator of Fouse Epidermal v.acromolecular Synthesis and Hyperplasia but with Weak Tumor-promoting Ability. J. Natl. Cancer Inst., 55: 983-987, 1975. Trosko, J. E., Yager, J. D., Bowden, G. T., and Butcher, F. R. The Effects of Several Croton Oil Derivatives on Two Types of DNA Repair and Cyclic Nucleotide Metabolism in *+aanalian Cells In Vitro. Chem.-Biol. Interact. 11: 191-205, 1975. 1976 Bo::t•aell, R. R. The Biochemistry of P:eneoplasia in Mouse Skin. Cancer Res., 35• 253'--2533, 1976. O'Brien, T. G. The Induction of Ornithine Decarboxylase as an Early, Possibly Cbliaatory, Ever,t in Mcuse Skin Carcinogenesis. Cancer Res., 36: 2644-2653, 1976. 0'B: ien, .. G., Si Ms_'-,.a.^., R. C., and Bcut.+ell, R. K. The Effect of Colchicine cn t':e Ind;:c:ioz of O:nit.hine Decarboxylase by 12-0-Tetradecanoyl-Phorbol- 13-Acetate. Cancer Res., 36: 3766-3770, 1976. Poirier, L. A., and Boutwell, R. K. Current Problems in Nutrition and Cancer. Fed. Proc., 35: 1307-1308, 1976. 1977 Boutwell, R. K. The Role of the Induction of Ornithine Decarboxylase in Tumor Promotion. In: H.H. Hiatt, J.D. Watson, and J.A. Winsten (Eds.), Origi.n,s of Human Cancer, pp. 773-783. New York: Cold Soring Harbor Laboratory, 1977. DeYoung, L. M., Mufson, R. A., and Boutwell, R. K. An Apparent Inactivation of Ir.--.ated Cells by the Potent Inhibitor of 'I`.+o-Stage Mouse Skin T:.mor gAnes. , Bis(2-c.^.loroethyl)sulfi-_Je. Cancer Res., 37: 4590-4594, 1977. Nufsor„ o. ,,., Si^sinan, R. C., and Boutwell, R. K. The Effect of the Phorbol .-z.-. .,:r.o: Pro:r,oters on the Basal and Catecholamine-stimulated Levels of Cyclic Ader,osine 3':5'-!'cnoohosphate in Mouse Skin and Epidermis. Cancer Res., 37: 665-669, 1977. CTR t Et'`t 010923

Mufson, R. A., Astrup, E. G., Simsiman, R. C., and Boutwell, R. K. Dissociation of Increases in 3':5'-Ader.osine Monophosphate and 3':5'-Guanosine Mono- phosphate from the Ir.d_cticn of Ornithine Decarboxylase by 12-0-Tetra- decanoyl-phorbol-13-acetate in Mouse Epidermis. Proc. Natl. Acad. Sci., U.S.A., 74: 657-661, 1977. Mufson, R. A., DeYoung, L. M., and Boutwell, R. K. Microwave Irradiation: A New Rapid Technique for Separating Epidermal Tissue from Mouse Skin Preserving RNA, DNA, Protein, and Phorbol Ester-Induced Ornithine Decarboxylase Levels. J. Invest. Derm., 69: 547-550, 1977. Raineri, R., Simsiman, R. C., and Boutwell, R. K. Stimulation of the Synthesis of Mouse Epidermal Histones by Tumor-promoting Agents. Cancer Res., 37: 4584-4589, 1977. Slaga, T. J., and Bout-well, R. K. Inhibition of the Tumor-initiating Ability of the Potent Carcinogen 7,12-Dimethylbenz(a)anthracene by the Weak Tumor Initiator 1,2,3,4-Dibenzanthracene. Cancer Res., 37: 128-133, 1977. Verma, A. K., and Boutwell, R. K. Vitamin A Acid (Retinoic Acid), a Potent Inhibitor of 12-0-Tetradecanoyl-phorbol-13-acetate Induced Ornithine Decarboxylase Activity in Mouse Epidermis. Cancer Res., 37: 2196-2201, 1977. Verma, A. K., Rice, °. M., and Boutwell, R. K. Prostaglandins and Skin Tw.or Promotion: Inhibition of T:~nor Promoter-Induced Ornithine Decarboxylase Activi:y in Lpiaerais by Inhibitors of Prostaglandin Synthesis. Biochim. Biophfs. Res. C_-=rn., 79: 1160-1166, 1977. 1978 Boutwell, R. K. Biochemical `!echanisms of Tumor Promotion. In: T.J. Slaga, A. Sivak, and R.eC. Bo.:taell (Eds.), Carcinogenesis, Vol. 2, Mechanisns of Taror Promotion and C xarcinogenesis, pp. 49-58. New York: Raven Press, 1978. DeYoung, L. M., Richards, W. L., Bonzelet, W., Tsai, L. L., and Boutwell, R. K. Localization and Significance of ~-Glutaaryltranspeptidase in Normal and Neoplastic Mouse Skin. Cancer Res., 38: 3697-3701, 1978. Kensler, T. W., Verma, A. K., Boutwell, R. K., and Mueller, G. C. Effects of Retinoic Acid and Juvenile Hormone on the Induction of Ornithine Decarboxylase Activity by 12-0-Tetradecanoylphorbol-13-acetate. Cancer Res., 38: 2896- 2899, 1978. Lowe, N., Verma, A. K., and 3out:+e11, R. K. Ultraviolet Light Induces ^~1 C:n:.'::.^.e De^a_.,oxyl3se Act.vity. J. Invest. Derm., 7: 417-418, 1978. Mufson, R. A. Effects of ._...,. Promoters on Cyclic Nucleotide Metabol:sm in Mouse Skin and Eaider:is In Vivo. :n: R. J. Slaga, A. Sivak, and R. K. Boutwell (Eds.), Carcinogenesis, Vol. 2, Mechanisms of Tumor Promotion and Cocarci::oaAnesis, oo. 135-151. New York: Raven Press, 1973. CTR IFt l 't 010924

Raineri, R., Simsiman, R. C., and Boutwell, R. K. Stimulation of the Syrthesis of the H1 and H3 Histone Fractions of Mouse Epidermis by 12-0-Tetradecanoyl- phorbol-13-acetate. Cancer Lett., 5: 277-284, 1978. Verna, A. K., Rice, H. M., Shapas, B. G., and Boutwell, R. K. Inhibition of 12-0-Tetradecanoylphorbol-13-acetate-Induced Ornithine Decarboxylase Activity in Mouse Epidermis by Vitamin A Analogs (Retinoids). Cancer Res., 38: 793-801, 1978. 1979 Boutwell, R. K. and Verma, A. K. Effects of Vitamin A and Related Retinoids on the Biochemical Processes Linked to Carcinogenesis. Pure Appl. Cbera. 51: 857-866, 1979. Mufson, R. A., Sirosi^an, R. C., and Boutwell, R. K. Increased Cyclic Adenosine 3':5'-:^onoohosohate Phosphodiesterase Activity in Mouse Skin Papilloaa Epide-siis and Paorbol Ester-Treated Mouse Skin Epidermis. Cancer Rea., 39: 2036-2040, 1979. Verma, A. K., Lowe, N. J., and Boutwell, R. K. Induction of Mouse Epidersal Orn'_t:::ne Decarboxylase Activity and DNA Synthesis by Ultraviolet Light. Cancer Res., 39: 1035-1040, 1979. Verma, A. R., Shapas, B. G., Rice, H. M., and Boutwell, R. K. Correlation of the Inhibition bv Retinoids of Tu_nor Promoter-Induced Mouse Epidermal Ornit:ine Decar!5ox•flase Activity and of Skin Tumor Promotion. Cancer Res., 39: 419-:25, 1979. Kertz, ?. W., Kensler, T. W., Mueller, G. C., Verma, A. K., and Boutwell, R. K. 5,o-Epoxyretinoic Acid Crposes the Effects of 120Tetradecanoylphorbo113 acetate in in Bovine Ly-rp::ocytes. Nature, 277: 227-229, 1979. Mu?sor„ n. A., Fischer, S. M., Verma, A. K., Gleson, G. L., Slaga, T. J., and Bcutwe11, R. K. Effects of 12-0-Tetradecanoylphorbo1-13-acetate and riezerein on Epide:ma1 Ornithine Decarboxylase Activity, Isoproterenol-stimualted Levels of Cyclic Adenosine 3':S'-Monophosohate, and Induction of Mouse Skin Tu.nors In Vivo. Cancer Res., 39: 4791-4795, 1979. Boutwell, R. K., O'Brien, T. G., Verma, A. K., Weeks, R. G., DeYoung, L. li., Ashendel, C. L., and Astrup, E. G. On the Biochemical Mechanism of Tumor Promotion in Mous. Skin. In: E. C. Miller, J. A. Miller, I. Hirono, T. Sugimura, and S. Tikaya-za (Eds.), Naturally Occurring Carcinogens, Mutagens and Modulators of CarciTiogenesis, pp. 287-300. Baltimore: University Park Press, 1979. Bou;•.+e'_l,R. ?C. , O'Br ien, T. G., Verma, A. K., Weeks, R. G., DeYoung, L. !4. , Ashendel, ... :.., _nd Astrup, E. G. The Induction of Ornithine Decarboxylase Activity and Its Control in Mouse Skin Epidermis. Adv. Enz. Regulation 17: 89-112, 1979. Asnendel, C. L., and Boutwell, R. K. Prostaglandin E and F Levels in Mouse Epidermis are Increased by ':u.:,or-Yro^oting Phorbol Esters. Biochem. Biophys. Res. Co :^un., 90: 623-627, 1979. _.r i ir+ t! t-~ 0 1092 S

t Lowe, N., Verma, A. K., and 3cut:+e11, R. K. Ultraviolet Light Induces Epider"al O:nit!:ine Decarboxylase Activity. J. Invest. Dermatol., 71: 417-418, 1979. 1980 Verma, A. K., Ashendel, C. L., and Boutwell, R. K. Inhibition by Prostaglandin Synthesis Inhibitors of t.he Induction of Epidermal Ornithine Decarboxylase Activity, the Accmulation of Prostaglandins, and Tumor Promotion Caused by 12-0-Tetradecanoylphorbol-13-acetate. Cancer Res., 40: 308-315, 1980. DiGiovanni, J., Viaje, A., Fischer, S., Slaga, T. J., and Boutwell, R. K. Biotrans- formation of 7,12-Dimet.':ylbenz(a)anthracene by Mouse Epidermal Cell Culture. Carcinogenesis, 1: 41-49, 1980. Kishore, G. S., and Boutwell, R. K. Enzymatic Oxidation and Reduction of Retinal by Mouse Epidermis. Bioc.^.em. Biophys. Res. Comm., 94: 1044-1051, 1980. Verma, A. K., Slaga, T. J., Wertz, P. W., Mueller, G. C., and Boutwell, R. K. Inhibition of Skin T;..^~or Promotion by Retinoic acid and Its Metabolite, 5,6- Epoxy:etinoic Acid. Cancer Res., 40: 2367-2371, 1980. DiGiovanni, J., Berry, D. L., Gleason, G. L., Kishore, G. S., and Slaga, T. J. Ti-e-aeoender.t Ir.hibiticz by_2,3,7,3-Tetrachlorodibenzo-p-dioxin of Skin Tu-x>:igenes'_s with Polycyclic Hydrocarbons. Cancer Res., 40: 1580-1587, 1980. Ve:ma, A. K., and Boutwell, R. K. Effects of Dose and Duration of Treat.vent with the Tumor ?:oroting Agent, 12-0-Tetradecanoylphorbol-13-acetate on Mouse Skin Ca:cinogenesis. Carcizogenesis, 1: 271-276, 1980. Boutwell, R. K., and Haynes, H. A. Malignant and Benign Neoolasms of the Skin. J. inves;. Derm., 74: 443-451, 1979. DiGiovanni, J., Slaga, T. J., and Boutwell, R. K. Comparison of the Tumor Initiating Activitv of 7,12-Dimet!:v'_benz(a)anthracene and Benzo(a)pyrene in Female SENCA-R and CD-1 Mice. Carcinecenesis, 1: 381-389, 1980. ! .r t R t 1 } 1 0.t 0926