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b HKOSgS015 41870 2 ENHANCED CELLULAR BINDING AND PENETRATION OF NUCLEIC ACIDS CAUSED BY CHEMICAL AND PHYSICAL CARCINOGERS E~D MUTAGENS PROC AM ASSoc.CANcER RES 17(0): 8; 1976 KUBINSKI, H/ MORIN, NM, ZELDIN, PE/ WEIIIHOUSE, S, FOTI, M MEETING ABSTRACT: THE INITIAL OBSERVATION THAT A CARCINOGENIC CHEMICAL, 1,3-PROPANE SULTONE~ INCREASES BINDING AND PENETRATION OF DNA AND RNA TO EHRLICH ASCITES TUMOR CELLS IN VITRO PROMPTED A COMPARISON OF THE EFFECTS OF OTHER CARCINOGENS AND MUTAGENS IN'THE PRESENT INVESTIGATION, OVER 30 CHEMICALS WERE TESTED. FINDINGS: NUCLEIC ACID ATTACHMENT TO AND SUBSEQUENT PENETRATION INTO BOTH EUKARYOTIC AND PROKARYOTIC CELLS WAS SIGNIFICANTLY ENHANCED BY ALL ULTIMATE CARCINOGENS. FORMATION OF ARTIFICIAL NUCLEOPROTEINS BY ADDITION OF: A BASIC PROTEIN TO THE REACTION MIXTURE FURTHER INCREASED THE BINDING. !IONULTIMATE DERIVATIVES AND NONCARCINOGENIC AND NONMUTAGENIC CCMF'OUNDS HAD NO EFFECT, BUT PRECARCINOGENS AND INTERMEDIATE CARCINOGENIC FORMS WERE ACTIVATED BY RAT DR MOUSE LIVER EXTRACTS. DNA IRRADIATED WITH ULTRAVIOLET LIGHT REACTED WITH THE CELLS IN A MANNER SIMILAR TO THAT OF UNIRRADIATED CONTROL. IRRADIATION IN THE PRESENCE OF LYSOZYME, HOWEVER~ ENHANCED CELLULAR BINDING MORE THAN 20°FOLD. C~l-R 9B I]OHG 01057

HKOSgS016 41870 DISCUSSION: SUCH INCREASED ATTACHMENT AND PENETRATION OF NUCLEIC ACIDS CAUSED BY CHEMICAL AND PHYSICAL CARCINOGENS CAN PLAY ,'. ROLE DURING THE PROCESS OF TUMOR INDUCTION. OBSERVATIONS REPORTED HERE MAY FORM A 3ASI$ FOR DEVELOPING A SIMPLE AND REPRODUCIBLE TEST SYSTEM FOR SCREENING OF CARCINOGENIC AND MUTAGENIC COMPOUNDS. /VOS/ (PRESENTED AT THE 67TH ANNUAL ~ETIN6 OF THE ~RICAN ASSOCIATION FOR CANCER RESEARCH AND THE ~TH ANNUAL MEETING OF THE AMERICAN SOCIETY OF CLINICAL 0NCOLOGY, TORONTO, CANAJ~A, MAY 4-8, 1976,) U WISC, M~ISOR, WIS/ U WISC, MADISOR, WIS MEETING ABSTRACT, BIOCHEMICAL STUDY A, GENETIC STUDY A, IN VITRO STUDY A, R J~DIATION STUDY A/ CYTOCHEMICAL STUDY A, MICE, RATS, CARCINOOENICITY TESTING A, CARCINOGENESIS MECHANISMS A, CARCINOGENS BIOASSAY A, CHEMICAL CARCINOGENESIS, PHYSICAL CARCINOGENS, SCREENING TEST A, NUCLEIC ACID BINDING As D~IA BINDING, ~IA BINDING, PROTEIN BINDING, CHEMICAL BINDING, LIVER CELLS A, CARCINOGENS ACTIVATION A, CARCINOGENS ACTION MECHANISMS A, CARCINOGENS BIOLOGICAL ACTIVITY, CARCINOGENS METABOLIC EFFECTS A, NUCLEOPROTEIN A, CARCINOGENS POTENCY A, CARCINOGENS PROMOTERS A, CARCINOGENS CYTOTOXIClTY A, CHEMICAL MUTAGENS, CELL CHANGES A/ EHRLICH ASCITES TUMOR CELLS, CARCINOGENS CHEMICAL PROPERTIES, CARCINOGENS PHYSICAL PROPERTIES, NUCLEIC ACID METABOLISM DISORDERS, NUCLEIC ACID ~J3NORMALITIESj LIVER CELL CHANGES A, LIVER EXTRACTS A, CELL IRRADIATION A, CIR 9E~ CDHG 0"1050

HK0595~t7 41870 TUMOR INDUCTION HI, LYSOZYME A, CELL DNA CHANGES A, CELL R~A CHANGES A, CELL NUCLEIC ACID CHANGES A, CELL PROTEIN CHANGES, CARCINOGENS BINDI:rG A// ENGLISH LANGUAGE, MADISON WI£ RESIDENCE, USA RESXDENCE~ GRANTOR USPHS, CONFERENCE, TORONTO CANADA, AMER ASSN CANCER RE$, AMER SOC CLINICAL ONCOLOGY, TEMPLE UNIV C~TF~ 9B 130lql~ 01059