Tobacco Documents Online

OAH/CF/I20 PROJECT SHIP PECTZN - HZCOTZNE Z/FI'EIACTZOLq bT D.A. Ksckln ep Work c~trrtmd out st 3town & glll£amson Tobacco Company mnd £n CI~DC has shorn cbac pmcc£n (oecurlns naturally ta tobscco) can eff~c~encly scaven8e ucLcotlne. In s recent empez%menc 401 of atcottne in lamlua •as C~8nsferred Co mhmmt by th£s p~cmss. Zc has been malWemtod b7 I. Lltz£nSec ~t~C in the Fhll~p ~o~£s process Jppll~tion of dlaumon~zm phosphate cJ~OltJ ~t~qMJdJt of pectin f:em the ce~LuLer structure of :obscco. Zt is ~houKht ChAc dfJaoontum phosphate te decomposed £nco ammonia and monoam~o~umphosphace. The J.scer is quite 8c~Ldlc sad clesves the pectin l~uicaBes Co ceIluLs~ matert~l. Pectin conceits po17811AcC~tontc sctd (psrtts~ structure shorn he~o~), CO.~ L-.( and ls hence an scldlcmate~lml contzi~Lus ~rboxyllc acid St•ups. One possible mechanism of capture of n~coclne by peccLu ~s ~he forsmc~on of • ulcoc£nm-pmcc~n s~lc as ~lcot£n8 £s h~$h17 bss~c (scheme) P. gheuedcotlam ~e combined vLch pectin the release temperature of ~Leotlm~ may be ~lteredo Yh£s wm~ld of course effect the aff~clanc7 of • nlcot~n8 tracafo:. Prev~ou~ ~ork In GR~OC (1) has 8hog: chat the • mmperatute of n~cotlnm relies ~i~ dependent on the nature of the ulcot£ne. Nicotine free base Is released at compsr•bly lo~ c~mpmracures (max. 138°) ud~LZe nlcot~e Is released at • h£sher temperature (um~. 2~80) from the BAT Co LTD - MINNESOTA TOBACCO LITIGATION

-2- / o~a~nte. Other 88Zts such 8s the acetate, c£trate mad hydrochlor£de release nicot£ne at £nta~med48te temperatures. To check whether pect£n ¢ouId influence the temperature of ~Lcot£ne release, 8 1:1 utixture aud a 1:10 ~Lxture of n~t~u end pectin• z~spect~ve~y were prepared~. 7~i of Chase macadams ve~t injected into a IOwa secedes of paper f£1ter rod end pyrolysed st a hsstiu$ nee of of 1.6 ~-1 £u arson, usl~K the standard p3~olys~s/mass speoCromater ~eehn£que (2). The u~coc~ne pyrolysis pro~£1es are L3.Lascrated £n the attached £1~uree. For ~Lcoc4n8 b88a (coutroZ) the mazLumm r&te of transfer o¢¢ured at 165", ~en 8 1:1 -4~ra of Ucot£zut lad poct£n vu azam~nad • ma~4mum was found eKs~n at 105°, but 8dd~t£ou~y chars yes ~urcher rt~aaee o~ nicotine at h£shet teazpe~scuz~e C30O° mad 36A0)o On exau~L~at~ou of ~Lcotine w~Lth au excess of pect4n (1:10) release of n~cot:Lne oc~u~d ouly at ".he I~'Lsher tenperature (max. $67") and ao~e at all at 165°. Th~e e-~ur17 d~mouetraces thaC pecr~Ln £ncremsas the te~spe~mt~re at u~Lch ~L~oc~ne t~ft~ ers • The full Lmp~cac£ons of Chase prtl~z~ury studies are not understood and £C is recommended chac fuccbar work £n Ch£s area £8 cerr£ed out. A work proKrsm £s attached that is £utended to cover such york £or the nest 6 - 8 ~aeks. & commmrc~ly ~vs£~b~e fruit poctLn marketed under the trade name WCaz~ow . J~ m~thmm~c raaczl~ £msodt~t~Ly asumad on -4~nS tlsa pact£n and ~4 cot~t. BAT Co LTD - MINNESOTA TOBACCO LITIGATION ,,.,.,j O~,

- 4 - 1. Prepere ~Lcot~lt sables (.cetate, citrate etc.) and mix v:Lth pect£n. To test uhecher nlcof~Lne tms to be Ln the free base £ora. 2. Extend work to other larKe mole©ular wetSht compounds, such as 2arty colds and study cba£n lenstb ~l~£ects. 3." Add ~Lcotine-pect£u u=Lztures to ciptettes to ascet~a£n vhetlher mdLcot~m dal:Lvtr:lLes &re lncr~seod Ln the ma£utremm smoke. Meawart e£destt~um ~e~do. &. Rep/4~t of pect~Ln IrJLth • eult~b:le ~trbo~11c 8old besod tlmvo~r add~t~ aS, coum8~c er.~d, r.:Lm~l~¢ 8c~d etc. Th:Ls ~.~ by-pass 8x:Lst4n8 pettnts ou pectin and provide products w~Lth enltm~.ed flewour and 4nc:eaeed ~lLcot£ne y~elds. C~Lrculat ~Lon Dr. C.1. ~res Dr. I.L ]Laker Dr. IL.~. CreXlln Flie t20 t~ c~ -.J BAT Co LTD - MINNF_.~OTA TOBACCO LITIC, ATION