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A STATISTZC~ ANALYSZS OF JANUS ~ B10 ItEL'01~ 110. P~).1691 I~STILZCTI~ 14.9.1979 AUTHOR: E.B. Wflke$ ISSUED BY: S.I. Evelyn PROC. REF. : 11.O5.O2 --~ = =, Dc, S.J. Green Copy No. 1 Dr. D.C. Fetl~n " " 2, 3 Library " " 4, 5 COM SO.- r~ c~ 0 H ~ =o LTD - MINNESOTA TOBACCO LITIGATION

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Group Research & Development Centre, • ri c£sb-Ame~ican Tobacco Co. Ltd., SOUT~TON. 14ch September 1979 a sta z,s'rxc. Tsis me,.. s!o 0~porc Bo. RD.1691 Restrleted) SUMHAIY The bioloe~c•l obeerv•tlonJ o£ this axper~mmslt have already been rmpo~ted. This report deacrlb•a a 8tatlst£~al analysis o£ some o£ chm ~aca and the main conc~ue£on8 drawn are: 1 The cond~ssts from the control tobacco ot the ¥CL derived from ic are Zess acc£ve than chose from c~s•rette8 conta~ninS BA~14ark Z. 2 The dec• sul~aaC ChaC ~ Mark I ~ry be lees acClve chart the mod£fied version used in this ~cper~aenc. 3. On • per cLKaracce bas£8, chose coucs£n£nS ~kTFLA]~ Ma~k T are lass active than e£ther cha control tobacco or I~L cipreCtes. On the bax£s of these ~txults, and frou the r~ult8 obtained durinS the development ~ eva~u&t£on of ~Kt~k $Z, it £s recounendod chat I~TTIJL~Z Mark Z shouZd ~ot be inco~potata~ £nto riser@tree, Work on ~u~cher development was ten.hated some cona£dmrable time eZo. ~ ~ Zo LTD - MINNESOTA TOBACCO LITIGATION I O r~o O

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-2- 0 BJ ECT 1-~ES The objective of th~s experimmt was to compare the t~oriKenlc a~:¢ivitle8 of the condensate8 from eLK•reties made £rom PCL, and £rom ;:.safeties conta£ni=8 IA~TLAJ2, with that of the condensate from ~:..sarette8 contain/~$ the blend from which the ~ was made. An ,~itpainced acetone control group of animals was also included in Chls ~_~peri~eut to monitor the spontaneous skin turnout rate of the animals ~,:i ng used. DESIGN OF ZX~n .~r The control c£8arettas were made from flue-cured tobacco cuc at ~ cuts per inch for the lamlna and 160-180 cuts per inch for the ~.:e~; the 1ms;ha to stem ratio in the cigarettes was 60/AO. The PCL ~as cuC at 80 cuts per inch. The BATIrLA~ wee blended with the stem/ ~,,~ina and 8o was cut ac 60 cuts per inch. All ciKarette$ were 70 mm L~ length and 25 mm in c£rc,-sFerence, and were ~rapped in )ollor6 015 :,:~se paper. No f~lter tlp8 ere used. Two version of BAT~A~ were used. The first, labelled 'A* L~t th£u experiment, was Hark £ BATFLAKE containing 12Z o~ cellulosic ~,~er. The second, labelled 'BY in this e~peri~ent, ~a8 • modified arsion of Mark I BATTLAI~ s~d contained 2~Z o£ cellulosic binder. version 'Av was used at two inclusion levels, 251 and 5OZ of cigarette ~ei~ht. Version 'B' was used at one /~cluslon level, ~0~ of ciKarette ~iKht. Details of animal husbandry and tonal@users production are given ~ ~1). Three dose levels were used for the ¢ondensatem from the P~. and ~q~F[JU0S c£Karettes, and five dose lmll were used for the ~, ~ 2o LTD - MINNESOTA TOBACCO LITIGATION O

INDEX HAS INDICATED GAP IN BATES RANGE HERE

-3- condensate from the control cigarette. One group of animals was painted ~.ch acetone/water solvent containing no condensate, so that a total of ~,~ jroup8 of animals vtrt used. ~ roups Tdere = Tobacco control BIO/I PCL ex control B10/2 Control + 23% 'A' B10/3 Control + 50% 'B' B10/5 Control + 50Z 'A' B10/6 The numbers of animals in the various ,, m Dose Level 14.1 20.0 28.3 126 m 127 126 127 127 127 126 ~hus 2149 animals were exposed to condensate treatment. 40.0 56.6 126 127 127 126 126 126 126 126 126 127 The acetone/water s(>lveut painted group contained 84 an/~ale, making a total of 2233 animals ~I~ all. The numher of animals in the oriKinal deeiKnof the ezperiment yes ]26 for each o~ the 17 condensate treated Stoups, rather than 127 as ~ shown for 7 of the Stoups £n the above table. These extra 7 animals a~-e called "iZPLA~Rr ~S" and ware t~ken £nto the experiment to ]'eplaca those an/amls dy/JmK dur~ the £1=st tour weeks of t:~stzaent. '~te phrase "~ ~ IHCLUDZD" appearing at the top of ~4bles 1 to 36 shows that these replacement animals were included in ,:he analys£s. ~TS Or sxn!n~n~ The practice at Battelle, Fraukgurt. is to examine each animal weekly. 8k.Ln lesions w~thln the pei~ted area are recorded 8e tumours ! m CO ~ ~ ~o LTD - MINNESOTA TOBACCO LITIGATION