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-2- INTRODUCTION Planet ~_iKaretnes, produced by Courcaulds and incorpora~inK chair smoking material, were nest marketed in the Midlands area of the U.K. for three weeks lace in 1973. A previous report (i) gave detailed results obtained by them/tel, physical and microbiological examination of the smoking material and analysis of the smoke. Samples of the cigarettes were also sent ~o B-&.T. HamburE so than the smoke ¢ondensace could be exam/ned in the RMFI =asc and che sebaceous gland Case. The results of ~heae Casts (2) w~re received from H~mburK lace in 1974 and are given in this report. RESULTS AND DISCUSSION The JANUS sample B8-2 was included in the examination so that: the resu2cs of Planet cigarette could be compared with chose of a tobacco sheet maCer~al (X-PCL-5) incorporated, at the 60Z level, into a flue-cured blend. In addition co this sample, samples of Krone and R6 were included in the nests. The results obtained by B-A.T. Hamburg are sac ouC in Table i. TABLE I SEBACEOUS ~ AND NMFZ RESULTS Sample Planet R6 Krone B8-2 Condansane (ms/c£g) 9.3* 12.5 17.5 21.4 Sebaceous GLand We£ghC of CoudenmsCe for 5OZ Suppresa£on 39.5 24.3 21.6 25.7 Number of CiEaretces for 50! Suppression 4.24 1.94 1.23 1.20 BMFZ 5.65 4.2 5.2 4.6 * Some condensate was lost due to a sliKhc defect of the smoking machine. P BAT Co LTD - MINNESOTA TOBACCO LITIGATION

INDEX HAS INDICATED GAP IN BATES RANGE HERE

-3- The values given in Table i indicate that the weight of condensate to produce an equal biological response (i.e. 50% suppression of sebaceous glands) is much higher for condensate from Planet cigarettes than it is for the other samples: therefore, on the basis of this test, the biological activity of Planet condensate is appreciably lower than that of the other cigarettes. The low activity of Planet is also ~ndicated by the results on a 'per cigarette' basis slnce, for example, the condensate from 1.2 of the B8-2 clgareuces would produce the same sebaceous gland response as that from 4.2 Planet cigaret=es. ~n the lonE-term test at Battelle the tumorigenicity of condensate from sample B8-2 was found to be considerably lower than that from the control flue-cured blend which included 50Z CES; provisional JANUS results ind£ca~s that the low activity of B8-2 £s highly slgnificant statistically. Since the short-term sebaceous gland test correlates well wlch lonE-term experiments, the comparative values for Planet and B8-2 in this test predict that condensate from Plane= ~uld have a very low tumorlgeniclty if it were to be examined in a font-term test. In contrast Co the sebaceous @land results, the NMT~ test i~dlcates Chat the activity of the condensate from Planet is hLsher Chart chat of sample B8-2 and is also higher than condensaces from the ocher clgarecces examined ~n fihls series. Since NMFI values generally correlate well vith the results of lonE-term skin painting experiments and also with those from the sebaceous @land test, it is pertinent to consider possible ¢~planations for the contradictory results. There are ~wo possible reasons wh£ch should be considered. F£rsfily, results obCa£ned in Southampton have shown that BAT Co LTD - MINNESOTA TOBACCO LITIGATION m N

INDEX HAS INDICATED GAP IN BATES RANGE HERE

-4- condensaces from flue-cured cisarettes with elevated levels of ammonia have hiEhNMFI values. AlthouEh these values are consistent with the lonE-term tumoriEeuicity results for fermented tobaccos it is possible that an~onia, or nitroEenous deEradation products, could have an appreciable effect on the binding reaction with albumin which is an essential scaEe in the NMFI test. If this is the case it is probable that pTTolysls products from the urea present in the Courceulds smoklnE~arial would also affect the binding reaction. Secondly, it has recently been noted that particularly when there are differences in the ra=io of =he init£atlng and promotinE activity of condensetes the NMFI result must be treated wlth caution. For these reasons it is considered that the overall conclusion from these tests should be based on the in vivo sebaceous sland results. RE~RENCES I. B-A.T. Report No. ED.1099-R, 3.4.74. 2. Letter from B-A.T. NamburE, 11.11.74. BAT Co LTD - MINNESOTA TOBACCO LITIGATION